BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,071,884	T→C	T247A (ACC→GCC)	pgk ←	phosphoglycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,071,884	0	T	C	99.1%	420.5 / NA	116	T247A (ACC→GCC)	pgk	phosphoglycerate kinase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (58/57); total (58/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

AGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGT  >  NC_000913/3071750‑3072018

aGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCcagca                                                                                                                                   <  2:499659/140‑1 (MQ=255)

     aaGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGt                                                                                                                              >  1:334195/1‑140 (MQ=255)

       acATGACCGATATCCAAGATCTGCTCGTCAGCTTTCACATCGGTAACAGATTTCAGGGCAGCAGGTGCAGTTTCAGAGATCTCGTTTTCTTCGCGAACATCAGACGGAAACGGGGTGATGCAGTTTGCCAGCAGACtttt                                                                                                                            >  2:1251755/2‑140 (MQ=255)

          tCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGc                                                                                                                         <  2:1131277/140‑1 (MQ=255)

            aCCGATATACAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCtt                                                                                                                       <  2:890743/140‑1 (MQ=255)

               gATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAg                                                                                                                          <  1:1655761/134‑1 (MQ=255)

               gATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAg                                                                                                                          >  2:1655761/1‑134 (MQ=255)

                 tatCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCa                                                                                                                  <  1:794673/140‑1 (MQ=255)

                   tCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAAc                                                                                                                <  2:1135759/140‑1 (MQ=255)

                   tCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAAc                                                                                                                <  2:579048/140‑1 (MQ=255)

                   tCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAAc                                                                                                                <  1:824184/140‑1 (MQ=255)

                           cTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAg                                                                                                        >  1:972750/1‑140 (MQ=255)

                           cTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAg                                                                                                        >  1:936692/1‑140 (MQ=255)

                            tGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGc                                                                                                       <  2:1402532/140‑1 (MQ=255)

                             gCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGt                                                                                                           <  1:1693326/135‑1 (MQ=255)

                             gCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGt                                                                                                           >  2:1693326/1‑135 (MQ=255)

                             gCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCt                                                                                                      >  2:1551703/1‑140 (MQ=255)

                             gCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCt                                                                                                      >  2:1090977/1‑140 (MQ=255)

                             gCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCt                                                                                                      >  1:520217/1‑140 (MQ=255)

                             gCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCt                                                                                                      >  2:114057/1‑140 (MQ=255)

                             gCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCt                                                                                                      >  2:1029814/1‑140 (MQ=255)

                             gCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCt                                                                                                      >  1:1029039/1‑140 (MQ=255)

                              cTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCtt                                                                                                     >  1:1654096/1‑140 (MQ=255)

                              cTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGCGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCtt                                                                                                     >  2:914988/1‑140 (MQ=255)

                                cGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGACCATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCt                                                                                                      >  1:1467474/1‑137 (MQ=255)

                                cGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCt                                                                                                      <  2:1467474/137‑1 (MQ=255)

                                cGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCg                                                                                                   >  1:827749/1‑140 (MQ=255)

                                   cAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTAc                                                                                                >  2:1113609/1‑140 (MQ=255)

                                      cTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAgg                                                                                             <  2:936692/140‑1 (MQ=255)

                                               cGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGAGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTAccc                                                                                    <  2:1243543/140‑1 (MQ=255)

                                                gTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACa                                                                                               <  1:1166928/128‑1 (MQ=255)

                                                gTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCca                                                                                   <  1:1113609/140‑1 (MQ=255)

                                                  tAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCcaca                                                                                 <  2:37442/140‑1 (MQ=255)

                                                   aaCAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACAt                                                                                >  1:947510/1‑140 (MQ=255)

                                                   aaCAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACAt                                                                                >  1:12198/1‑140 (MQ=255)

                                                     cAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCg                                                                              <  1:417164/140‑1 (MQ=255)

                                                       gATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTg                                                                            >  2:1683293/1‑140 (MQ=255)

                                                        aTTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTgg                                                                           <  2:12198/140‑1 (MQ=255)

                                                         tttCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCca                                                                                   >  1:37766/1‑131 (MQ=255)

                                                         tttCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCca                                                                                   <  2:37766/131‑1 (MQ=255)

                                                         tttCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGc                                                                          >  1:783721/1‑140 (MQ=255)

                                                         tttCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGc                                                                          >  1:1116899/1‑140 (MQ=255)

                                                          ttCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGcc                                                                         <  2:1654096/140‑1 (MQ=255)

                                                                gTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGc                                                                   <  2:683728/140‑1 (MQ=255)

                                                                 tAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGcc                                                                  <  2:783721/140‑1 (MQ=255)

                                                                  aGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcg                                                                 <  2:1029039/140‑1 (MQ=255)

                                                                  aGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcg                                                                 <  2:947510/140‑1 (MQ=255)

                                                                   gCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcgc                                                                >  1:179245/1‑140 (MQ=255)

                                                                   gCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcgc                                                                >  2:1293010/1‑140 (MQ=255)

                                                                    ccGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCg                                                                              >  2:1405082/1‑125 (MQ=255)

                                                                    ccGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCg                                                                              <  1:1405082/125‑1 (MQ=255)

                                                                    ccGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcgcg                                                               >  2:834322/1‑140 (MQ=255)

                                                                    ccGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcgcg                                                               >  1:736870/1‑140 (MQ=255)

                                                                       gTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATa                                                            <  1:318755/140‑1 (MQ=255)

                                                                       gTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATa                                                            <  2:829285/140‑1 (MQ=255)

                                                                         gCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGAt                                                             >  1:1044016/1‑137 (MQ=255)

                                                                         gCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGAt                                                             <  2:1044016/137‑1 (MQ=255)

                                                                         gCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATaaa                                                          >  2:770665/1‑140 (MQ=255)

                                                                             tttCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGgtg                                                      >  2:703523/1‑140 (MQ=255)

                                                                             tttCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGgtg                                                      >  1:744379/1‑140 (MQ=255)

                                                                                  gagaACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGc                                                 <  1:1251755/140‑1 (MQ=255)

                                                                                  gagaACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGc                                                 >  2:372107/1‑140 (MQ=255)

                                                                                      aCTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATa                                             <  1:372107/140‑1 (MQ=255)

                                                                                       cTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATac                                            >  2:816293/1‑140 (MQ=255)

                                                                                       cTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATac                                            >  1:382941/1‑140 (MQ=255)

                                                                                       cTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTCAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATaa                                                           <  2:1236655/125‑1 (MQ=255)

                                                                                        tCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATacc                                           <  2:736870/140‑1 (MQ=255)

                                                                                          ggTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCg                                                >  1:1528413/1‑133 (MQ=255)

                                                                                          ggTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATaccac                                         >  2:445417/1‑140 (MQ=255)

                                                                                          ggTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATaccac                                         <  1:447849/140‑1 (MQ=255)

                                                                                             tGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATaccaccac                                      <  2:179245/140‑1 (MQ=255)

                                                                                              gCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATaccaccacc                                     <  2:165746/140‑1 (MQ=255)

                                                                                              gCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATaccaccacc                                     <  2:382941/140‑1 (MQ=255)

                                                                                               cTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATAccaccacca                                    >  1:816954/1‑140 (MQ=255)

                                                                                               cTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATAccaccacca                                    >  2:759436/1‑140 (MQ=255)

                                                                                                  cgcgAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACcaaca                                 <  2:364924/140‑1 (MQ=255)

                                                                                                  cgcgAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACcaaca                                 <  1:69610/140‑1 (MQ=255)

                                                                                                       aCATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAg                            <  1:445417/140‑1 (MQ=255)

                                                                                                              aCGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCa                     >  1:1164959/1‑140 (MQ=255)

                                                                                                               cGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAGCCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAg                    <  1:1017427/140‑1 (MQ=255)

                                                                                                               cGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAg                    <  2:1696222/140‑1 (MQ=255)

                                                                                                               cGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAg                    <  1:816293/140‑1 (MQ=255)

                                                                                                               cGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAg                    <  2:1164959/140‑1 (MQ=255)

                                                                                                               cGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAg                    <  1:1683293/140‑1 (MQ=255)

                                                                                                                   aCCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAt                >  1:326750/1‑140 (MQ=255)

                                                                                                                   aCCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAt                >  1:414312/1‑140 (MQ=255)

                                                                                                                     cGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAttt              >  1:67093/1‑140 (MQ=255)

                                                                                                                     cGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGAttt              >  1:600991/1‑140 (MQ=255)

                                                                                                                        gATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAg           <  2:804481/140‑1 (MQ=255)

                                                                                                                            ttGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAg       >  2:497950/1‑140 (MQ=255)

                                                                                                                            ttGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAg       >  1:47477/1‑140 (MQ=255)

                                                                                                                            ttGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAg       <  1:709913/140‑1 (MQ=255)

                                                                                                                             tGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAgg      <  2:47477/140‑1 (MQ=255)

                                                                                                                             tGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAgg      <  1:114057/140‑1 (MQ=255)

                                                                                                                               cAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGa    >  2:675919/1‑140 (MQ=255)

                                                                                                                                 ggtggcCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGt  <  2:816954/140‑1 (MQ=255)

                                                                                                                                 ggtggcCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGt  >  1:471077/1‑140 (MQ=255)

                                                                                                                                  gtggcCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCg                  <  2:1345105/123‑1 (MQ=255)

                                                                                                                                  gtggcCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCg                  >  1:1345105/1‑123 (MQ=255)

                                                                                                                                  gtggcCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCg                  >  1:84543/1‑123 (MQ=255)

AGCGGAAGCATCACCGATATCCAGGATCTGCTCGTCAGCTTTCACATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGATACCACCACCAACAATCAGCTGGTCAGCGATTTTAGACAGGGAGT  >  NC_000913/3071750‑3072018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`