Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,196,006	G→A	P250S (CCA→TCA)	rfaE ←	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,196,006	0	G	A	100.0%	295.1 / NA	83	P250S (CCA→TCA)	rfaE	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/39);  total (44/39)

ACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACAT  >  NC_000913/3195876‑3196139                                                                                                                                   |                                                                                                                                      acGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAg                                                                                                                              <  2:820295/140‑1 (MQ=255)   gCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAgcg                                                                                                                            <  1:810048/140‑1 (MQ=255)      aGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAgcggcg                                                                                                                         <  2:501354/140‑1 (MQ=255)       gccgccGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGgcgc                                                                                                                        >  2:340786/1‑140 (MQ=255)       gccgccGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGgcgc                                                                                                                        >  1:831304/1‑140 (MQ=255)        ccgccgCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCt                                                                                                                       >  1:560959/1‑140 (MQ=255)            cgcATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTAc                                                                                                                   <  1:7118/140‑1 (MQ=255)                      aagaagCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTAc                                                                                                                   <  2:546626/130‑1 (MQ=255)                      aagaagCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTAc                                                                                                                   >  1:546626/1‑130 (MQ=255)                      aagaagCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTgcag                                                                                                         <  2:261956/140‑1 (MQ=255)                        gaagCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTgcagca                                                                                                       <  2:831304/140‑1 (MQ=255)                        gaagCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTgcagca                                                                                                       <  2:508247/140‑1 (MQ=255)                         aagCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGcagcag                                                                                                      >  1:1067600/1‑140 (MQ=255)                           gCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCg                                                                                                    >  2:879728/1‑140 (MQ=255)                             aGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGAc                                                                                                  <  1:651117/140‑1 (MQ=255)                                 ttcttcCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATAc                                                                                              >  2:1219243/1‑140 (MQ=255)                                   cttcCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTgcag                                                                                                         >  2:616026/1‑127 (MQ=255)                                   cttcCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTgcag                                                                                                         <  1:616026/127‑1 (MQ=255)                                   cttcCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATAccc                                                                                            >  1:1108125/1‑140 (MQ=255)                                       cAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGtt                                                                                        <  2:1067600/140‑1 (MQ=255)                                         gCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCg                                                                                      >  1:1683465/1‑140 (MQ=255)                                         gCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCg                                                                                      >  1:819439/1‑140 (MQ=255)                                         gCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCg                                                                                      >  2:417605/1‑140 (MQ=255)                                           gAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTGACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGa                                                                                    >  2:1305002/1‑140 (MQ=255)                                             aTTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAAc                                                                                  <  2:1094530/140‑1 (MQ=255)                                               tACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACgg                                                                                <  1:1219243/140‑1 (MQ=255)                                               tACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACgg                                                                                <  1:417605/140‑1 (MQ=255)                                                     cTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAAc                                                                                  <  1:638570/132‑1 (MQ=255)                                                     cTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAAc                                                                                  >  2:638570/1‑132 (MQ=255)                                                      tGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTa                                                                         >  2:923416/1‑140 (MQ=255)                                                         cAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACa                                                                      <  1:923416/140‑1 (MQ=255)                                                         cAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACa                                                                      <  2:85896/140‑1 (MQ=255)                                                            cGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACag                                                                     >  2:136884/1‑138 (MQ=255)                                                            cGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACag                                                                     <  1:136884/138‑1 (MQ=255)                                                                gccgccAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTa                                                                         >  1:467091/1‑130 (MQ=255)                                                                gccgccAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTa                                                                         <  2:467091/130‑1 (MQ=255)                                                                gccgccAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACa                                                                      <  2:1655489/133‑1 (MQ=255)                                                                gccgccAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACa                                                                      >  1:1655489/1‑133 (MQ=255)                                                                gccgccAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCga                                                               >  1:764414/1‑140 (MQ=255)                                                                gccgccAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCga                                                               >  1:668549/1‑140 (MQ=255)                                                                 ccgccAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCgag                                                              <  2:1683465/140‑1 (MQ=255)                                                                 ccgccAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCgag                                                              <  2:764414/140‑1 (MQ=255)                                                                  cgccAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCgaga                                                             >  1:856893/1‑140 (MQ=255)                                                                                  aCCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAAt                                             <  1:182702/140‑1 (MQ=255)                                                                                   ccGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATc                                            >  2:516140/1‑140 (MQ=255)                                                                                      tgtCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGt                                         >  1:1696564/1‑140 (MQ=255)                                                                                         cGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTc                                      >  2:1225133/1‑140 (MQ=255)                                                                                         cGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTc                                      >  1:1631318/1‑140 (MQ=255)                                                                                           cccGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCAt                                    >  2:430408/1‑140 (MQ=255)                                                                                           cccGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCAt                                    >  1:972160/1‑140 (MQ=255)                                                                                           cccGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCAt                                    >  2:1203731/1‑140 (MQ=255)                                                                                            ccGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATg                                   <  1:340786/140‑1 (MQ=255)                                                                                            ccGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATg                                   <  1:1203731/140‑1 (MQ=255)                                                                                            ccGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATg                                   <  2:183559/140‑1 (MQ=255)                                                                                             cGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGc                                  >  1:1461610/1‑140 (MQ=255)                                                                                                   ggTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCt                            <  2:1108125/140‑1 (MQ=255)                                                                                                    gTAACGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTc                           <  1:1093196/140‑1 (MQ=255)                                                                                                        cGTCATACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTcaaca                       <  2:856893/140‑1 (MQ=255)                                                                                                            aTACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAAtct                   >  2:840653/1‑140 (MQ=255)                                                                                                            aTACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAAtct                   <  2:819439/140‑1 (MQ=255)                                                                                                            aTACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAAtct                   >  1:702298/1‑140 (MQ=255)                                                                                                            aTACACTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAAtct                   >  2:696556/1‑140 (MQ=255)                                                                                                              acacTTCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATctct                 >  2:894268/1‑140 (MQ=255)                                                                                                                  ttCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTcttctt             >  1:1333586/1‑140 (MQ=255)                                                                                                                  ttCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTcttctt             <  1:516140/140‑1 (MQ=255)                                                                                                                  ttCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTcttctt             <  1:430408/140‑1 (MQ=255)                                                                                                                  ttCCTGCGCTCGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTcttctt             <  1:696556/140‑1 (MQ=255)                                                                                                                   tCCTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCttcttc            >  1:185586/1‑140 (MQ=255)                                                                                                                    ccTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTcaaca                       <  1:1023964/128‑1 (MQ=255)                                                                                                                    ccTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTcaaca                       >  2:1023964/1‑128 (MQ=255)                                                                                                                    ccTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTca                          >  1:1561001/1‑125 (MQ=255)                                                                                                                    ccTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTca                          <  2:1561001/125‑1 (MQ=255)                                                                                                                    ccTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCg           >  1:488347/1‑140 (MQ=255)                                                                                                                    ccTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCg           >  1:1587847/1‑140 (MQ=255)                                                                                                                     cTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCg           >  1:868410/1‑139 (MQ=255)                                                                                                                     cTGCGCTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCg           <  2:868410/139‑1 (MQ=255)                                                                                                                       gcgcTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTc        >  1:261682/1‑140 (MQ=255)                                                                                                                          cTTGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTa     >  1:662250/1‑140 (MQ=255)                                                                                                                            tGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACa   >  2:1038332/1‑140 (MQ=255)                                                                                                                            tGGGTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACa   >  1:841075/1‑140 (MQ=255)                                                                                                                             gggTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACAt  <  2:1631318/140‑1 (MQ=255)                                                                                                                             gggTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACAt  <  1:3902/140‑1 (MQ=255)                                                                                                                             gggTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACAt  <  1:1305002/140‑1 (MQ=255)                                                                                                                             gggTTGACATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACAt  <  2:972160/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                      ACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACAT  >  NC_000913/3195876‑3196139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑