Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,281,898	T→C	intergenic (+273/‑78)	agaA → / → agaS	putative truncated N‑acetylgalactosamine‑6‑phosphate deacetylase/putative galactosamine‑6‑phosphate deaminase/isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,281,898	0	T	C	100.0%	287.1 / NA	78	intergenic (+273/‑78)	agaA/agaS	putative truncated N‑acetylgalactosamine‑6‑phosphate deacetylase/putative galactosamine‑6‑phosphate deaminase/isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/44);  total (34/44)

AAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGAC  >  NC_000913/3281762‑3282022                                                                                                                                         |                                                                                                                             aaGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGAcaccac                                                                                                                           <  2:820991/140‑1 (MQ=255)    cGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCaa                                                                                                                        >  1:1041871/1‑140 (MQ=255)       ttGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGtg                                                                                                                     >  2:1094006/1‑140 (MQ=255)       ttGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGtg                                                                                                                     >  2:1451006/1‑140 (MQ=255)       ttGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGtg                                                                                                                     >  2:1639523/1‑140 (MQ=255)        tGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGtga                                                                                                                    >  1:476337/1‑140 (MQ=255)         gcgcCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGtgaa                                                                                                                   <  1:1005954/140‑1 (MQ=255)              gcATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGtgaattgaa                                                                                                              >  1:1030421/1‑140 (MQ=255)               taTCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGtgaaatgaaa                                                                                                             <  1:198540/139‑1 (MQ=255)               cATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGtgaaatgaaa                                                                                                             <  1:1613018/140‑1 (MQ=255)                 tCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACg                                                                                                           <  1:1257497/140‑1 (MQ=255)                 tCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACg                                                                                                           <  1:909647/140‑1 (MQ=255)                  ccAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGa                                                                                                          <  2:1638544/140‑1 (MQ=255)                  ccAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGa                                                                                                          <  2:1559027/140‑1 (MQ=255)                  ccAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTCAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGa                                                                                                          <  2:1159036/140‑1 (MQ=255)                      cAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAgg                                                                                                      <  1:1451006/140‑1 (MQ=255)                         tCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCaa                                                                                                   >  1:977553/1‑140 (MQ=255)                           ccTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGt                                                                                                 <  2:1229160/140‑1 (MQ=255)                           ccTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGt                                                                                                 >  2:248630/1‑140 (MQ=255)                                     ccTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACa                                                                                       >  1:1356929/1‑140 (MQ=255)                                     ccTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACa                                                                                       >  2:666658/1‑140 (MQ=255)                                       tttATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAAc                                                                                     >  1:491125/1‑140 (MQ=255)                                         tATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGc                                                                                   <  2:756847/140‑1 (MQ=255)                                          aTCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGcc                                                                                  >  2:1520447/1‑140 (MQ=255)                                          aTCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTCCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGcc                                                                                  <  1:1237750/140‑1 (MQ=255)                                           tcttttctttCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGccc                                                                                 <  1:6415/140‑1 (MQ=255)                                           tcttttctttCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGccc                                                                                 <  2:1659857/140‑1 (MQ=255)                                            cttttctttCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCg                                                                                <  1:806453/140‑1 (MQ=255)                                               ttctttcAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACg                                                                             <  1:1069355/140‑1 (MQ=255)                                               ttctttcAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACg                                                                             >  1:18875/1‑140 (MQ=255)                                                 ctttcAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTc                                                                           <  2:1217858/140‑1 (MQ=255)                                                  tttcAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCa                                                                          >  2:696493/1‑140 (MQ=255)                                                   ttcAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCaa                                                                         <  1:763567/140‑1 (MQ=255)                                                   ttcAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCaa                                                                         <  2:127988/140‑1 (MQ=255)                                                    tcAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAg                                                                        <  2:491125/140‑1 (MQ=255)                                                    tcAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAg                                                                        <  1:765563/140‑1 (MQ=255)                                                         gATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTtcat                                                                   <  2:274398/140‑1 (MQ=255)                                                           tCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTtca                                                                    >  1:323532/1‑137 (MQ=255)                                                           tCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTtca                                                                    <  2:323532/137‑1 (MQ=255)                                                               aaaTTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACt                                                             <  2:1030421/140‑1 (MQ=255)                                                                    tCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACGAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGa                                                        >  2:1474095/1‑140 (MQ=255)                                                                    tCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGa                                                        <  1:1639523/140‑1 (MQ=255)                                                                     cGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGAt                                                       >  1:1245588/1‑140 (MQ=255)                                                                       ttttATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGAt                                                       >  2:633033/1‑138 (MQ=255)                                                                       ttttATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGAt                                                       <  1:633033/138‑1 (MQ=255)                                                                                  ttttCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAg                                          <  2:1245588/140‑1 (MQ=255)                                                                                   tttCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGa                                         <  2:18875/140‑1 (MQ=255)                                                                                    ttCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGaa                                        <  1:1474095/140‑1 (MQ=255)                                                                                       tcCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAAt                                     <  1:248630/140‑1 (MQ=255)                                                                                                   tCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCctgct                         >  1:1418173/1‑140 (MQ=255)                                                                                                   tCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCctgct                         >  2:699527/1‑140 (MQ=255)                                                                                                   tCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCctgct                         >  1:1156638/1‑140 (MQ=255)                                                                                                      gTTTCTTTTCTATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTgcc                      >  2:102903/1‑140 (MQ=255)                                                                                                            tttctATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCa                >  2:825681/1‑140 (MQ=255)                                                                                                             ttctATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCaa               <  1:1520447/140‑1 (MQ=255)                                                                                                             ttctATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCaa               >  2:397415/1‑140 (MQ=255)                                                                                                              tctATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAAc              <  2:723671/140‑1 (MQ=255)                                                                                                               ctATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCGAcc             <  2:1156638/140‑1 (MQ=255)                                                                                                               ctATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAAcc             <  2:1422222/140‑1 (MQ=255)                                                                                                               ctATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAAcc             <  1:666658/140‑1 (MQ=255)                                                                                                               ctATAGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAAcc             <  1:699527/140‑1 (MQ=255)                                                                                                                 ataGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCgg           >  1:991803/1‑140 (MQ=255)                                                                                                                  taGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGt          >  1:582325/1‑140 (MQ=255)                                                                                                                  taGATTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGt          <  1:825681/140‑1 (MQ=255)                                                                                                                     aTTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACa       >  2:73651/1‑140 (MQ=255)                                                                                                                     aTTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACa       >  2:1145411/1‑140 (MQ=255)                                                                                                                     aTTTTAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACa       >  2:878144/1‑140 (MQ=255)                                                                                                                       tttAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATg     <  1:878144/140‑1 (MQ=255)                                                                                                                       tttAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATg     <  1:397415/140‑1 (MQ=255)                                                                                                                       tttAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATg     >  1:18825/1‑140 (MQ=255)                                                                                                                       tttAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATg     <  2:808034/140‑1 (MQ=255)                                                                                                                         tAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAAcc             <  2:1547517/130‑1 (MQ=255)                                                                                                                         tAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAAcc             >  1:1547517/1‑130 (MQ=255)                                                                                                                         tAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGa   <  2:189052/140‑1 (MQ=255)                                                                                                                         tAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGa   <  2:18825/140‑1 (MQ=255)                                                                                                                         tAATCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGa   >  2:913278/1‑140 (MQ=255)                                                                                                                          aaTCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGAc  >  1:913988/1‑140 (MQ=255)                                                                                                                          aaTCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGAc  >  2:818900/1‑140 (MQ=255)                                                                                                                          aaTCAACGAAAGACACCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGAc  <  1:1145411/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                             AAGCGTTTGCGCCGCATCCAGCAATCCCTTTTGCTTCCTTTATCTTTTCTTTCAACGATCACAAATTTCGTTTTATTTCTTTTTTCTCCATTGAACTTTCAGTTTCTTTTCTATAGATTTTAATCAACGAAAGACATCACCAAGTGAAATGAAACGAAAGGCAAGTGAAAGCGACAACGCCCGACGTCAAGTTCATCAGACTAAGGATTGAGTTATGCCAGAAAATTACACCCCTGCTGCCGCCGCAACCGGTACATGGAC  >  NC_000913/3281762‑3282022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑