Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,474,861 (G)5→6 coding (641/861 nt) pdxI → pyridoxine 4‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,474,8561.G99.0% 717.8 / ‑3.1 201L212L (CTC→CTG) pdxIpyridoxine 4‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (98/101);  minor base A (1/1);  total (99/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 225 total aligned reads displayed.

GACGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑GGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGA  >  NC_000913/1474718‑1474983
                                                                                                                                           |                                                                                                                               
 aCGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑gg                                                                                                                               >  1:1526684/1‑140 (MQ=255)
  cGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCTCGCTC‑ggg                                                                                                                              <  1:1801954/140‑1 (MQ=255)
  cGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              <  1:2867948/140‑1 (MQ=255)
  cGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              <  2:3225591/140‑1 (MQ=255)
  cGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              <  1:1832201/140‑1 (MQ=255)
  cGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              <  2:1764604/140‑1 (MQ=255)
  cGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              <  1:3262533/140‑1 (MQ=255)
  cGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              <  2:2628261/140‑1 (MQ=255)
  cGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              <  2:816251/140‑1 (MQ=255)
  cGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              <  2:1367547/140‑1 (MQ=255)
  cGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              <  2:1191764/140‑1 (MQ=255)
    cAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggggg                                                                                                                            <  2:2900425/140‑1 (MQ=255)
          gCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTa                                                                                                                       <  2:1548295/140‑1 (MQ=255)
                gcgcgcAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGc                                                                                                                 >  1:203629/1‑140 (MQ=255)
                 cgcgcAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTacac                                                                                                                    <  2:1039883/136‑1 (MQ=255)
                 cgcgcAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCt                                                                                                                >  1:377640/1‑140 (MQ=255)
                 cgcgcAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              <  2:2750117/125‑1 (MQ=255)
                 cgcgcAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              <  2:1778114/125‑1 (MQ=255)
                 cgcgcAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              >  1:1778114/1‑125 (MQ=255)
                  gcgcAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTg                                                                                                               <  1:1624720/140‑1 (MQ=255)
                   cgcAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGc                                                                                                              >  1:2303415/1‑140 (MQ=255)
                   cgcAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑ggg                                                                                                                              >  2:737672/1‑123 (MQ=255)
                    gcAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCa                                                                                                             <  1:615406/140‑1 (MQ=255)
                                  aTTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACttt                                                                                               >  2:2489627/1‑140 (MQ=255)
                                   ttGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTc                                                                                              >  2:514792/1‑140 (MQ=255)
                                          tgtgCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGtt                                                                                       >  1:1114797/1‑140 (MQ=255)
                                            tgCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTtgc                                                                                     >  1:104887/1‑140 (MQ=255)
                                            tgCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTtgc                                                                                     >  2:872645/1‑140 (MQ=255)
                                                aaaCGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCg                                                                                 >  2:1938103/1‑140 (MQ=255)
                                                   cGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGc                                                                              <  2:377640/140‑1 (MQ=255)
                                                    gAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGcc                                                                             >  1:772284/1‑140 (MQ=255)
                                                     aaTACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCt                                                                            <  2:1513740/140‑1 (MQ=255)
                                                      aTACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTg                                                                           <  2:2965755/140‑1 (MQ=255)
                                                         caacaTCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGt                                                                        >  1:612795/1‑140 (MQ=255)
                                                          aacaTCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTg                                                                       <  1:3382859/140‑1 (MQ=255)
                                                           acaTCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGc                                                                      <  1:2583479/140‑1 (MQ=255)
                                                           acaTCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGc                                                                      <  1:1748949/140‑1 (MQ=255)
                                                           acaTCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGc                                                                      <  1:366057/140‑1 (MQ=255)
                                                              tCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAac                                                                   <  1:2213610/140‑1 (MQ=255)
                                                               cgcgCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCg                                                                                 >  1:1364672/1‑125 (MQ=255)
                                                               cgcgCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAaca                                                                  <  1:453664/140‑1 (MQ=255)
                                                               cgcgCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAaca                                                                  >  1:1238941/1‑140 (MQ=255)
                                                                gcgcACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAacac                                                                 >  2:1879491/1‑140 (MQ=255)
                                                                 cgcACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACAcc                                                                >  1:27580/1‑140 (MQ=255)
                                                                    aCCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAAt                                                             <  2:1238941/140‑1 (MQ=255)
                                                                    aCCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAAt                                                             <  1:2817279/140‑1 (MQ=255)
                                                                     ccGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATg                                                            <  1:2086689/140‑1 (MQ=255)
                                                                      cGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGc                                                           >  2:2925113/1‑140 (MQ=255)
                                                                        tGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTCCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAg                                                         >  1:160576/1‑140 (MQ=255)
                                                                             atgatgCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGgc                                                    <  2:1114797/140‑1 (MQ=255)
                                                                                  gCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTgg                                               >  2:938801/1‑140 (MQ=255)
                                                                                   cAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGc                                              <  1:969234/140‑1 (MQ=255)
                                                                                   cAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGc                                              <  2:1607809/140‑1 (MQ=255)
                                                                                    aaTGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCg                                             >  1:301514/1‑140 (MQ=255)
                                                                                    aaTGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCg                                             >  2:2332448/1‑140 (MQ=255)
                                                                                     aTGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGt                                            <  1:2489627/140‑1 (MQ=255)
                                                                                      tgattgatGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTg                                           <  2:2303415/140‑1 (MQ=255)
                                                                                       gattgatGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTgg                                          <  1:1916027/140‑1 (MQ=255)
                                                                                           gatGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGgcg                                                   <  1:658691/127‑1 (MQ=255)
                                                                                           gatGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGgcg                                                   >  2:658691/1‑127 (MQ=255)
                                                                                           gatGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGt                                      >  1:332558/1‑140 (MQ=255)
                                                                                            atGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGATGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGtt                                     >  2:156670/1‑140 (MQ=255)
                                                                                              gCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTAc                                   >  2:3405111/1‑140 (MQ=255)
                                                                                                  tGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCg                               >  2:472403/1‑140 (MQ=255)
                                                                                                  tGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCg                               >  1:2665409/1‑140 (MQ=255)
                                                                                                  tGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCg                               >  1:2683655/1‑140 (MQ=255)
                                                                                                  tGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCg                               >  2:2154515/1‑140 (MQ=255)
                                                                                                  tGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCg                               >  2:2076803/1‑140 (MQ=255)
                                                                                                  tGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCg                               <  2:1677740/140‑1 (MQ=255)
                                                                                                   ggCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGATGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGt                              <  1:156670/140‑1 (MQ=255)
                                                                                                    gCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGt                                            >  1:3412524/1‑125 (MQ=255)
                                                                                                    gCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGtt                             >  1:720723/1‑140 (MQ=255)
                                                                                                     cccACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTc                            <  2:3146032/140‑1 (MQ=255)
                                                                                                      ccACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCa                           >  2:1061022/1‑140 (MQ=255)
                                                                                                        aCGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCAcc                         <  2:3430709/140‑1 (MQ=255)
                                                                                                         cGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCg                        >  2:1849696/1‑140 (MQ=255)
                                                                                                               cATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATAtt                  <  1:3307695/140‑1 (MQ=255)
                                                                                                                   gCCTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGc              <  1:792878/140‑1 (MQ=255)
                                                                                                                    ccTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             <  2:2665409/140‑1 (MQ=255)
                                                                                                                    ccTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             <  2:612795/140‑1 (MQ=255)
                                                                                                                    ccTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             <  2:301514/140‑1 (MQ=255)
                                                                                                                    ccTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             <  1:1026623/140‑1 (MQ=255)
                                                                                                                    ccTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             <  1:2591939/140‑1 (MQ=255)
                                                                                                                    ccTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             <  2:197568/140‑1 (MQ=255)
                                                                                                                    ccTACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             >  2:1363140/1‑140 (MQ=255)
                                                                                                                      tACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             <  1:2369096/138‑1 (MQ=255)
                                                                                                                      tACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGa           >  1:1400674/1‑140 (MQ=255)
                                                                                                                      tACGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGa           <  2:2164251/140‑1 (MQ=255)
                                                                                                                        cGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             <  2:2393519/136‑1 (MQ=255)
                                                                                                                        cGTGCCGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGAGTTCACCGAATATTTTGCTGATc         >  2:415034/1‑140 (MQ=255)
                                                                                                                            ccGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAg     >  1:585245/1‑140 (MQ=255)
                                                                                                                             cGTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACGGCGTTCACCGAATATTTTGCTGATCCCAgg    <  2:332558/140‑1 (MQ=255)
                                                                                                                              gTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACTCCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             <  1:198394/130‑1 (MQ=255)
                                                                                                                              gTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAggg   <  2:203629/140‑1 (MQ=255)
                                                                                                                              gTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAggg   <  1:1061022/140‑1 (MQ=255)
                                                                                                                              gTTCTTCCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAggg   <  2:2683655/140‑1 (MQ=255)
                                                                                                                               ttcttcCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAggg   >  1:1457242/1‑139 (MQ=255)
                                                                                                                               ttcttcCCGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAggg   <  2:1457242/139‑1 (MQ=255)
                                                                                                                                    cccGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             <  1:888706/124‑1 (MQ=255)
                                                                                                                                    cccGCTCGGGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCt             >  2:888706/1‑124 (MQ=255)
                                                                                                                                           |                                                                                                                               
GACGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGTGTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCCCACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTC‑GGGGGCTTTACACCGCTGCAATCGTCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCGTGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGA  >  NC_000913/1474718‑1474983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑