BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	255,174	(C)_6→7	coding (543/1458 nt)	pepD ←	peptidase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	255,168	1	.	C	99.3%	486.3 / ‑4.0	135	coding (549/1458 nt)	pepD	peptidase D
Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (68/66); total (68/67)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

GTTTGTTGGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGC  >  NC_000913/255029‑255300

gTTTGTTGGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑a                                                                                                                                        <  1:1800618/140‑1 (MQ=255)

    gttgGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccc                                                                                                                                  <  2:1965111/140‑1 (MQ=255)

    gttgGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccc                                                                                                                                  <  2:895947/140‑1 (MQ=255)

     ttgGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑ccccc                                                                                                                                 <  2:2473582/140‑1 (MQ=255)

     ttgGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑ccccc                                                                                                                                 <  2:1437725/140‑1 (MQ=255)

     ttgGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑ccccc                                                                                                                                 <  1:1484431/140‑1 (MQ=255)

      tgGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccccc                                                                                                                                >  1:2353886/1‑140 (MQ=255)

      tgGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccccc                                                                                                                                >  1:2582948/1‑140 (MQ=255)

         cATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccccccgc                                                                                                                             <  1:563121/140‑4 (MQ=255)

           ttACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccccccgcac                                                                                                                           <  1:1787578/140‑6 (MQ=255)

           ttACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccccccgcac                                                                                                                           <  2:2490325/140‑6 (MQ=255)

           ttACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccccccgcac                                                                                                                           <  1:2469368/140‑6 (MQ=255)

             aCCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACaa                                                                                                                          <  1:2804035/140‑1 (MQ=255)

             aCCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACaa                                                                                                                          <  2:1107186/140‑1 (MQ=255)

             aCCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACaa                                                                                                                          <  2:3037901/140‑1 (MQ=255)

             aCCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccccccg                                                                                                                              >  2:1787634/1‑133 (MQ=255)

             aCCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccccccg                                                                                                                              <  1:632673/135‑3 (MQ=255)

             aCCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccccccg                                                                                                                              >  2:632673/1‑133 (MQ=255)

              cccAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAAc                                                                                                                         >  2:809520/1‑140 (MQ=255)

              cccAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAAc                                                                                                                         <  2:3301380/140‑1 (MQ=255)

                  gCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCAt                                                                                                                     >  2:3432022/1‑140 (MQ=255)

                   cccAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATg                                                                                                                    >  1:947092/1‑140 (MQ=255)

                   cccAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATg                                                                                                                    >  1:1790332/1‑140 (MQ=255)

                        cGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCAt                                                                                                                     >  2:3438035/1‑134 (MQ=255)

                        cGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGAt                                                                                                               >  1:27190/1‑140 (MQ=255)

                        cGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGAt                                                                                                               >  2:875180/1‑140 (MQ=255)

                        cGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑cccccccgc                                                                                                                             >  2:1808496/1‑122 (MQ=255)

                         gTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACaa                                                                                                                          <  2:933218/128‑1 (MQ=255)

                             aTTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAg                                                                                                                 <  1:928666/133‑1 (MQ=255)

                                tCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACctt                                                                                                       <  2:636582/140‑1 (MQ=255)

                                    cgccgGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACcttcttc                                                                                                   >  2:2758506/1‑140 (MQ=255)

                                      cgggAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACcttcttctt                                                                                                 >  2:2412497/3‑140 (MQ=255)

                                      ccgGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACcttcttctt                                                                                                 <  1:2758506/140‑1 (MQ=255)

                                        ggAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCg                                                                                               <  1:2412497/140‑1 (MQ=255)

                                        ggAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCg                                                                                               <  1:2027774/140‑1 (MQ=255)

                                            tGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGt                                                                                           <  2:1990664/140‑1 (MQ=255)

                                            tGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGt                                                                                           <  2:299398/140‑1 (MQ=255)

                                              aCCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCg                                                                                         <  2:2361848/140‑1 (MQ=255)

                                              aCCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCg                                                                                         >  2:2594970/1‑140 (MQ=255)

                                                   cTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGtt                                                                                    <  2:2292056/140‑1 (MQ=255)

                                                   cTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGtt                                                                                    <  1:809520/140‑1 (MQ=255)

                                                    tttCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGATGC‑‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTa                                                                                   <  2:1343594/140‑1 (MQ=255)

                                                    tttCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTa                                                                                   <  1:2594970/140‑1 (MQ=255)

                                                    tttCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTATTCGGAGTCGGTGTTa                                                                                   >  1:2981749/1‑140 (MQ=255)

                                                      tCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAAt                                                                                 <  1:1941754/140‑1 (MQ=255)

                                                          aCCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGt                                                                                           <  1:2827177/126‑1 (MQ=255)

                                                             ttttAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAata                                                                          >  1:2442624/1‑140 (MQ=255)

                                                               ttAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATc                                                                        <  2:3222131/140‑1 (MQ=255)

                                                                 aaGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAg                                                                      >  2:328525/1‑140 (MQ=255)

                                                                      tAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGc                                                                 >  2:1739518/1‑140 (MQ=255)

                                                                      tAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTATTCGGAGTCGGTGTTAATCAGAATATCAGCCTGc                                                                 <  2:2981749/140‑1 (MQ=255)

                                                                       aaCTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCa                                                                >  1:475786/1‑140 (MQ=255)

                                                                        aCTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACTATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCaa                                                               >  1:2447646/1‑140 (MQ=255)

                                                                        aCTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTg                                                                  >  1:431204/1‑137 (MQ=255)

                                                                         cTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAAc                                                              <  2:2442624/140‑1 (MQ=255)

                                                                                  ttCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGccc                                                     >  2:2834095/1‑140 (MQ=255)

                                                                                  ttCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGccc                                                     >  1:1260848/1‑140 (MQ=255)

                                                                                  ttCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGccc                                                     >  1:979090/1‑140 (MQ=255)

                                                                                  ttCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGc                                                       <  1:382611/138‑1 (MQ=255)

                                                                                  ttCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGc                                                       >  2:382611/1‑138 (MQ=255)

                                                                                   tCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCt                                                    >  1:689362/1‑140 (MQ=255)

                                                                                     aaaaCCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCGCCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGt                                                  <  2:2582948/140‑1 (MQ=255)

                                                                                     aaaaCCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGt                                                  <  2:2353886/140‑1 (MQ=255)

                                                                                            gCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGa                                           >  2:2938573/1‑140 (MQ=255)

                                                                                             cTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGaa                                          <  2:475786/140‑1 (MQ=255)

                                                                                             cTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGaa                                          >  1:2764751/1‑140 (MQ=255)

                                                                                                 aaCCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa                                      <  1:3189996/140‑1 (MQ=255)

                                                                                                 aaCCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa                                      <  2:2764751/140‑1 (MQ=255)

                                                                                                 aaCCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa                                      <  1:1839916/140‑1 (MQ=255)

                                                                                                      cTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa                                      >  1:3111423/1‑135 (MQ=255)

                                                                                                      cTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa                                      <  1:819421/135‑1 (MQ=255)

                                                                                                      cTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTc                                 >  2:286565/1‑140 (MQ=255)

                                                                                                       ttCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa                                      >  2:1885061/1‑134 (MQ=255)

                                                                                                       ttCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTcc                                >  1:1196734/1‑140 (MQ=255)

                                                                                                           cGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCa                                      <  1:2237190/130‑1 (MQ=255)

                                                                                                            gATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATAcc                           <  2:1604700/140‑1 (MQ=255)

                                                                                                            gATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATAcc                           <  2:1293305/140‑1 (MQ=255)

                                                                                                              tCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCgg                         >  1:2074198/1‑140 (MQ=255)

                                                                                                              tCTAAATGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCgg                         <  1:568698/140‑1 (MQ=255)

                                                                                                                 aaaTGCAGGTTGGAGGTGAAGTCGAT‑ACCCCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGct                       >  2:2145019/1‑140 (MQ=255)

                                                                                                                    tGCAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGcttctt                   <  1:994466/140‑1 (MQ=255)

                                                                                                                      cAGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCg                 >  1:1528156/1‑140 (MQ=255)

                                                                                                                       aGGTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCgg                <  1:2282363/140‑1 (MQ=255)

                                                                                                                        ggTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGcttctt                   >  1:2914581/1‑136 (MQ=255)

                                                                                                                        ggTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGt               >  1:701019/1‑140 (MQ=255)

                                                                                                                        ggTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGt               >  1:1867129/1‑140 (MQ=255)

                                                                                                                        ggTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGt               >  1:2414703/1‑140 (MQ=255)

                                                                                                                         gTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTc              >  2:2447433/1‑140 (MQ=255)

                                                                                                                         gTTGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTc              <  1:3032167/140‑1 (MQ=255)

                                                                                                                          ttGGAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCa             >  2:1721795/1‑140 (MQ=255)

                                                                                                                            ggAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATg           >  1:2989767/1‑140 (MQ=255)

                                                                                                                            ggAGGTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATg           >  2:3091609/1‑140 (MQ=255)

                                                                                                                               ggTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTc        <  2:1196734/140‑1 (MQ=255)

                                                                                                                                gTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTca       <  2:2544268/140‑1 (MQ=255)

                                                                                                                                gTGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTca       <  2:549477/140‑1 (MQ=255)

                                                                                                                                 tGAAGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcag      >  1:3233218/1‑140 (MQ=255)

                                                                                                                                   aaGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcagca    >  2:2253523/1‑140 (MQ=255)

                                                                                                                                   aaGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcagca    >  2:280121/1‑140 (MQ=255)

                                                                                                                                   aaGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcagca    >  1:337214/1‑140 (MQ=255)

                                                                                                                                    aGTCGAT‑AC‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTcagcag   <  2:1260848/140‑1 (MQ=255)

GTTTGTTGGCATTACCCAGCCCAACGTGGATTTCCCCGCCGGAGTGACCGCCTTTCAGACCTTTTAAGGTTAACTTGAAGGTTTCAAAACCAGCTGGAACCGCTTCACGATCTAAATGCAGGTTGGAGGTGAAGTCGAT‑A‑‑CCCCCCGCACAACCCATGTAGATTTCACCTTCTTCTTCGGAGTCGGTGTTAATCAGAATATCAGCCTGCAACCAGTTGCCCTGTAAGCCGAACGCACCGTCCATACCGGCTTCTTCGGTCATGGTCAGCAGC  >  NC_000913/255029‑255300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`