BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,543	(C)_8→9	coding (492/732 nt)	pgeF ←	polyphenol oxidase YfiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,535	1	.	C	94.0%	388.6 / 14.1	116	coding (500/732 nt)	pgeF	polyphenol oxidase YfiH
Reads supporting (aligned to +/- strand): ref base . (3/4); new base C (54/55); total (57/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCT‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCG  >  NC_000913/2734396‑2734669

gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCt                                                                                                                                          >  2:2086039/1‑140 (MQ=255)

gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCt                                                                                                                                          <  1:2069380/140‑1 (MQ=255)

gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCt                                                                                                                                          >  1:2634736/1‑140 (MQ=255)

gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCt                                                                                                                                          >  1:1817693/1‑140 (MQ=255)

      gAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCT‑‑cccccc                                                                                                                                  <  2:2964194/140‑1 (MQ=255)

      gAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCT‑‑cccccc                                                                                                                                  <  2:2190168/140‑1 (MQ=255)

      gAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCT‑‑cccccc                                                                                                                                  <  2:1268835/140‑1 (MQ=255)

         aaTTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCT‑‑ccccccccc                                                                                                                               <  1:194636/140‑2 (MQ=255)

            ttGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCT‑‑cccccccccact                                                                                                                            <  1:2127523/140‑5 (MQ=255)

                 cAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGaa                                                                                                                        <  1:2965443/140‑1 (MQ=255)

                  aaCACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAAc                                                                                                                       <  1:3291127/140‑1 (MQ=255)

                     ccccACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCACGAA‑CCTC‑CCCCCCCCACTTCGAAcgcg                                                                                                                    <  1:2411699/139‑1 (MQ=255)

                         aCGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTg                                                                                                                <  1:1370504/140‑1 (MQ=255)

                            ttCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGAc                                                                                                             <  2:2634736/140‑1 (MQ=255)

                             tCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGAcc                                                                                                            <  1:2982232/140‑1 (MQ=255)

                               gCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCaa                                                                                                          >  2:2252613/1‑140 (MQ=255)

                                   gACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                                      <  1:176639/140‑1 (MQ=255)

                                   gACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                                      <  1:1346718/140‑1 (MQ=255)

                                   gACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                                      <  1:2383268/140‑1 (MQ=255)

                                    aCGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                                      <  1:2623479/140‑1 (MQ=255)

                                     cGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAGAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCg                                                                                                    >  2:3010413/1‑140 (MQ=255)

                                             ggCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAc                                                                                            <  1:1414899/140‑1 (MQ=255)

                                               cAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCa                                                                                          <  1:1674517/140‑1 (MQ=255)

                                                    tGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCga                                                                                     >  2:339405/1‑140 (MQ=255)

                                                      aTAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCT‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGaa                                                                                  >  1:2236066/1‑140 (MQ=255)

                                                              ccGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCT‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTcc                                                                          <  2:410356/140‑1 (MQ=255)

                                                                gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCg                                                                         >  1:2038301/1‑140 (MQ=255)

                                                                gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCg                                                                         >  1:3144872/1‑140 (MQ=255)

                                                                gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCg                                                                         >  1:1811618/1‑140 (MQ=255)

                                                                 ccAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCgg                                                                        >  1:2097608/1‑140 (MQ=255)

                                                                  cAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAACCGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGa                                                                       <  1:2303620/140‑1 (MQ=255)

                                                                    gATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGAtt                                                                     <  1:1335217/140‑1 (MQ=255)

                                                                     aTACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTa                                                                    >  1:395564/1‑140 (MQ=255)

                                                                     aTACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCT‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTAt                                                                   >  1:1238041/1‑140 (MQ=255)

                                                                      tACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTAt                                                                   <  2:2097608/140‑1 (MQ=255)

                                                                      tACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTAt                                                                   >  1:3277/1‑140 (MQ=255)

                                                                          tATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGc                                                               >  2:2017749/1‑140 (MQ=255)

                                                                           aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCa                                                              >  2:531827/1‑140 (MQ=255)

                                                                              aCCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCaaaa                                                           >  2:3229648/1‑140 (MQ=255)

                                                                               ccATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAc                                                          >  1:1901944/1‑140 (MQ=255)

                                                                               ccATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAc                                                          >  1:1059510/1‑140 (MQ=255)

                                                                                  tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg                                                        >  1:3350502/1‑140 (MQ=255)

                                                                                  tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGCCCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGAtt                                                                     >  2:3062518/1‑126 (MQ=255)

                                                                                  tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGAtt                                                                     <  1:3062518/126‑1 (MQ=255)

                                                                                  tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCa                                                              <  1:1144100/133‑1 (MQ=255)

                                                                                  tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCAAGAATATTTTCCGGATTATCAGCa                                                              >  2:1144100/1‑133 (MQ=255)

                                                                                   gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                                                      >  2:2260758/1‑140 (MQ=255)

                                                                                   gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                                                      >  2:3108895/1‑140 (MQ=255)

                                                                                   gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                                                      >  2:1321603/1‑140 (MQ=255)

                                                                                    cTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaa                                                     >  1:3403750/1‑140 (MQ=255)

                                                                                    cTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaa                                                     >  2:1993805/1‑140 (MQ=255)

                                                                                     tgaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa                                                    <  1:2260758/140‑1 (MQ=255)

                                                                                       aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa                                                    >  1:3373650/1‑138 (MQ=255)

                                                                                       aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa                                                    <  2:3373650/138‑1 (MQ=255)

                                                                                       aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAcc                                                  >  1:3164789/1‑140 (MQ=255)

                                                                                       aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAcc                                                  <  2:2393628/140‑1 (MQ=255)

                                                                                       aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAcc                                                  >  2:2764601/1‑140 (MQ=255)

                                                                                       aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAcc                                                  >  1:2430085/1‑140 (MQ=255)

                                                                                       aatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAAcc                                                  >  1:2305514/1‑140 (MQ=255)

                                                                                         tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGt                                                <  2:806664/140‑1 (MQ=255)

                                                                                         tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGt                                                <  1:3300585/140‑1 (MQ=255)

                                                                                             aGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTctct                                            >  1:492170/1‑140 (MQ=255)

                                                                                                 gCACTTGCTTTAGCGTCTACTGCCAAAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtc                                               <  1:2926288/133‑1 (MQ=255)

                                                                                                 gCACTTGCTTTAGCGTCTACTGCCAAAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtc                                               >  2:2926288/1‑133 (MQ=255)

                                                                                                  cACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAg                                       >  1:3154840/1‑140 (MQ=255)

                                                                                                    cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAGAACAGGAAACCGTCTCTTCCAGCa                                     <  1:3010413/140‑1 (MQ=255)

                                                                                                    cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa                                     <  2:443726/140‑1 (MQ=255)

                                                                                                    cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa                                     <  2:2430085/140‑1 (MQ=255)

                                                                                                            aGCGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACCCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTgcg                             >  1:575807/1‑140 (MQ=255)

                                                                                                             gCGTCTACTGCCATAAACGCCGCGCGAA‑CCT‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           <  1:872509/140‑1 (MQ=255)

                                                                                                              cGTCTATTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           <  1:2491357/140‑1 (MQ=255)

                                                                                                              cGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTgcgga                           <  2:1059510/140‑3 (MQ=255)

                                                                                                              cGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           <  2:395564/140‑1 (MQ=255)

                                                                                                              cGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           <  1:382264/140‑1 (MQ=255)

                                                                                                              cGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           <  2:3403750/140‑1 (MQ=255)

                                                                                                              cGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           <  1:339405/140‑1 (MQ=255)

                                                                                                              cGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           <  2:1901944/140‑1 (MQ=255)

                                                                                                              cGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           <  1:2017749/140‑1 (MQ=255)

                                                                                                              cGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           <  2:3154840/140‑1 (MQ=255)

                                                                                                              cGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           <  1:3108895/140‑1 (MQ=255)

                                                                                                              cGTCTACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGCCTCTTCCAGCACGCCTGCGca                           <  1:531827/140‑1 (MQ=255)

                                                                                                              cGTCTACTGCCATAAACGCCTCGCAAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           <  1:1662649/140‑1 (MQ=255)

                                                                                                                  tACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGt                       >  2:641112/1‑140 (MQ=255)

                                                                                                                  tACTGCCATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTCCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGt                       >  2:2416869/1‑140 (MQ=255)

                                                                                                                       ccATAAACGCCTCGCGAA‑CCTCCCCCCCCCCACTTGGAACGCGCGTGGTCCAATTGCGGCCCTTACCCAGCGGAAAATATTTTCCGGTTTATCGGCAAAACAGGAAACCGTCCGTTCCAGCACGCCTGCGCACATGCCAc                   >  2:1620828/1‑140 (MQ=255)

                                                                                                                        cATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgc                 <  1:2785717/140‑1 (MQ=255)

                                                                                                                        cATAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgc                 <  1:2764601/140‑1 (MQ=255)

                                                                                                                         aTAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                           >  1:2403972/1‑129 (MQ=255)

                                                                                                                          tAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgcca               <  2:3164789/140‑1 (MQ=255)

                                                                                                                          tAAACGCCTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgc                 >  2:1769207/1‑138 (MQ=255)

                                                                                                                           aaaCGCCTCGCGAA‑CCTCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgcca               <  1:1620828/140‑1 (MQ=255)

                                                                                                                                 cTCGCGAACCCC‑‑CCCCCCCCCCTTCGAACGCGCGTGGACCAATTGCGGGCCCTAACCAGGGGGGAATATTTTCCGGTTTATCGGCAAAACAGGAAACCTTCTCTTCCAGCACGCCTCCGCACATTCCACCCCACCCAGCa        >  2:2423042/1‑140 (MQ=255)

                                                                                                                                 cTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAATCAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCa        >  2:1335867/1‑140 (MQ=255)

                                                                                                                                 cTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCa        >  1:140800/1‑140 (MQ=255)

                                                                                                                                 cTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCa        >  1:1268360/1‑140 (MQ=255)

                                                                                                                                 cTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCa        >  2:1529467/1‑140 (MQ=255)

                                                                                                                                 cTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCa        >  2:1907295/1‑140 (MQ=255)

                                                                                                                                 cTCGCGAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTT‑CAGCACGCCTGCGCACAGTCCACGCCAGCCAGCAt       >  1:3236356/1‑140 (MQ=255)

                                                                                                                                   cgcgAA‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATg      >  1:3484564/1‑140 (MQ=255)

                                                                                                                                       aa‑CCTC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAgcg  <  1:52722/140‑1 (MQ=255)

GCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑CCT‑‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCATGAGCG  >  NC_000913/2734396‑2734669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`