Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913650,8090TG27.5% 220.6 / 39.3 109T350P (ACC→CCC) citCcitrate lyase synthetase
Reads supporting (aligned to +/- strand):  ref base T (34/45);  new base G (30/0);  total (64/45)
Fisher's exact test for biased strand distribution p-value = 5.33e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 115 total aligned reads displayed.

CGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATATC  >  NC_000913/650675‑650948
                                                                                                                                      |                                                                                                                                            
cGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCtttt                                                                                                                                         >  2:549218/1‑140 (MQ=255)
  ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTCTTTTTATTTTTCCCCTCCTTCTCATTCCGGGGGCCTTTgg                                                                                                                                       >  1:569103/1‑139 (MQ=255)
  ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTTTCATGCGGGGGGCTTTTGa                                                                                                                                       >  2:845925/1‑140 (MQ=255)
  ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGa                                                                                                                                       >  1:731495/1‑140 (MQ=255)
     ctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGTCTTTTGACGa                                                                                                                                    >  2:1150929/1‑140 (MQ=255)
      tATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAg                                                                                                                                   >  1:2853232/1‑140 (MQ=255)
          gATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGc                                                                                                                               >  1:3482036/1‑140 (MQ=255)
               gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGt                                                                                                                          >  2:954519/1‑140 (MQ=255)
               gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTCACCTGTTTCTCCTGCGGGGGGCTTTTGACGAGCTGCcgcgg                                                                                                                          >  2:1396497/1‑139 (MQ=255)
                  ggTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTg                                                                                                                                <  2:1276735/131‑1 (MQ=255)
                  ggTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTg                                                                                                                                >  1:1276735/1‑131 (MQ=255)
                    tCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTgg                                                                                                                     <  1:1630941/140‑1 (MQ=255)
                    tCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTgg                                                                                                                     <  1:1202225/140‑1 (MQ=255)
                       atatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCgg                                                                                                                  <  1:3478556/140‑1 (MQ=255)
                        tatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCggg                                                                                                                 <  1:325144/140‑1 (MQ=255)
                         atCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGa                                                                                                                <  2:2309015/140‑1 (MQ=255)
                            cTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTg                                                                                                             <  1:845925/140‑1 (MQ=255)
                            cTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCAGGAGTg                                                                                                             <  2:1960841/140‑1 (MQ=255)
                             tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAgggg                                                                                                            >  1:538808/1‑137 (MQ=255)
                             tGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCgggggggg                                                                                                            >  1:377788/1‑135 (MQ=255)
                               cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGAggggtc                                                                                                          >  1:575186/1‑135 (MQ=255)
                               cGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCggggggggtc                                                                                                          >  2:3350370/1‑133 (MQ=255)
                                  aTCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGCTCCAg                                                                                                       >  1:809959/1‑140 (MQ=255)
                                  aTCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCaaa                                                                                                       >  1:1833166/1‑139 (MQ=255)
                                  aTCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAg                                                                                                       >  1:625761/1‑140 (MQ=255)
                                   tCGAGTGGGGCGATGGGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGc                                                                                                      <  1:152274/140‑1 (MQ=255)
                                     gAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAg                                                                                                    <  1:661214/140‑1 (MQ=255)
                                      aGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCCAGCAgg                                                                                                   >  2:1025840/1‑140 (MQ=255)
                                      aGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGCTTTTTTTTATTTTTCTCCTGTCTTTCATGCGCGGGTCTTTTGGCGGGCCGCCGCGTCCCGGCGGGAGGACTCAAGCAgg                                                                                                   >  2:1038253/1‑140 (MQ=255)
                                       gTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGt                                                                                                  <  1:1934825/140‑1 (MQ=255)
                                       gTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGt                                                                                                  >  1:3230187/1‑140 (MQ=255)
                                        tGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGtt                                                                                                 <  2:1407006/140‑1 (MQ=255)
                                          gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt                                                                                               <  2:619673/140‑1 (MQ=255)
                                          gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt                                                                                               <  2:2853232/140‑1 (MQ=255)
                                          gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt                                                                                               <  2:3230187/140‑1 (MQ=255)
                                          gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt                                                                                               <  1:1126488/140‑1 (MQ=255)
                                           ggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTTATTTTTCACCTGTTTTTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAgggtttt                                                                                              >  2:661214/1‑135 (MQ=255)
                                            gagacaggTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑c                                                                                            <  1:3144326/133‑1 (MQ=255)
                                             ggggatgTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTTTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCCAGCAGGGTTTG‑cc                                                                                           >  2:2509324/7‑139 (MQ=255)
                                              gATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑Caa                                                                                          <  1:2823499/140‑1 (MQ=255)
                                                  cGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAaataa                                                                                      <  2:6339/140‑1 (MQ=255)
                                                  cGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGCAGGTTTTT‑CAaataa                                                                                      >  2:574020/1‑140 (MQ=255)
                                                     agcatcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGc                                                                                   <  1:1779568/138‑1 (MQ=255)
                                                          cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGt                                                                              <  1:1150929/140‑1 (MQ=255)
                                                            cagccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGa                                                                            <  1:292640/137‑1 (MQ=255)
                                                            caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTTATTTTTCCCCTGTTTCTCATTCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAAGGTCTGCCAAAAAATGcgcggga                                                                             >  2:1667273/1‑134 (MQ=255)
                                                            caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTTTCCTTCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACAGGGTCTG‑CAAATAATGCAgggggg                                                                            >  2:245029/1‑135 (MQ=255)
                                                              tCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGTTCTG‑CAAAAAATGCAggtgtctt                                                                          >  2:3144326/1‑133 (MQ=255)
                                                               ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAaataa                                                                                      <  2:3469638/127‑1 (MQ=255)
                                                               ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAaataa                                                                                      >  1:3469638/1‑127 (MQ=255)
                                                                 ccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACAGGGTTTG‑CAAATAATTCAGCGGGGCTGCa                                                                       >  1:878627/1‑140 (MQ=255)
                                                                  cAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGGTTTT‑CAAAAAAAGCAGGGGGGCTGCAg                                                                      >  2:3045382/1‑140 (MQ=255)
                                                                       tCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGCAGGGTCTT‑CAAAAAAAGCAGCGGGACTGCCGGGGcc                                                                 >  2:284006/1‑140 (MQ=255)
                                                                       tCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGGTCTG‑CAAAAAAAGCAACGTGGCTGCAGGGAcc                                                                 >  2:1937921/1‑140 (MQ=255)
                                                                         aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAg                                                               >  1:3192539/1‑140 (MQ=255)
                                                                         aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGGTCTG‑CAAATAAAGCAGCGGGACTGCAGGGGCCAg                                                               >  1:577301/1‑140 (MQ=255)
                                                                          aGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAACAGGGTTTG‑CAAAAAATGCAGCGGGGCTGCAGGGGccccg                                                              >  2:292640/1‑137 (MQ=255)
                                                                          aGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAgc                                                              <  1:1497449/140‑1 (MQ=255)
                                                                              tGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAgcggcg                                                          >  1:2259365/1‑140 (MQ=255)
                                                                               gCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGGTCAAGCAGGGTCTG‑CAAAAAATGCAGCGTGACTGCCGGGACCAGCGgcgc                                                         >  2:2257723/1‑140 (MQ=255)
                                                                               gCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGCAGGGTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGgcgc                                                         >  1:3116761/1‑140 (MQ=255)
                                                                                ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg                                                        >  1:3319091/1‑140 (MQ=255)
                                                                                ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGGTCTT‑CAAAAAAAGCAGCGGGGCTGCAGGGACCCGCGGcgcg                                                        >  2:3368952/1‑140 (MQ=255)
                                                                                ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg                                                        >  1:1748577/1‑140 (MQ=255)
                                                                                  tGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGAt                                                      <  2:3192539/140‑1 (MQ=255)
                                                                                  tGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGAt                                                      <  1:2140910/140‑1 (MQ=255)
                                                                                  tGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGAt                                                      <  1:574020/140‑1 (MQ=255)
                                                                                   gCAACGGCGGGCTGGTTTTTTTTTATTTTTCACCTGTTTTTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGCAGGGTTTG‑CAAATAAATCAGCGTGACTGCAGGGGCCAGCGGCGCGGTa                                                     >  2:1919726/1‑140 (MQ=255)
                                                                                            ggCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGGTCTG‑CAAATAAAGCAGCGTGAATGCAGGGGCCAGCGGCGCGGTAGGCGTggga                                            >  2:859439/1‑140 (MQ=255)
                                                                                                   ttATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGc                                                   >  2:1095399/1‑126 (MQ=255)
                                                                                                   ttATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGc                                                   <  1:1095399/126‑1 (MQ=255)
                                                                                                        ttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGcc                                <  1:1919726/140‑1 (MQ=255)
                                                                                                          cATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcag                              <  1:1667273/140‑1 (MQ=255)
                                                                                                          cATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcag                              <  1:245029/140‑1 (MQ=255)
                                                                                                           aTTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc                             <  1:284006/140‑1 (MQ=255)
                                                                                                              tttCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGt                          <  1:1025840/140‑1 (MQ=255)
                                                                                                                 cACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGa                       <  2:2259365/140‑1 (MQ=255)
                                                                                                                       tttCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGctag                              <  2:1669585/127‑4 (MQ=255)
                                                                                                                       tttCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCtag                              >  1:1669585/1‑127 (MQ=255)
                                                                                                                       tttCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTAcc                 >  2:3165788/1‑140 (MQ=255)
                                                                                                                         tctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCg               >  2:2737164/1‑140 (MQ=255)
                                                                                                                         tctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCg               >  1:1841420/1‑140 (MQ=255)
                                                                                                                         tctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCg               >  2:1958489/1‑140 (MQ=255)
                                                                                                                            cATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGa            >  2:2144119/1‑140 (MQ=255)
                                                                                                                              tGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAg          >  1:2798350/1‑140 (MQ=255)
                                                                                                                              tGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAg          <  2:569103/140‑1 (MQ=255)
                                                                                                                              tGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAg          >  2:1587373/1‑140 (MQ=255)
                                                                                                                                cGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCg        >  1:890757/1‑140 (MQ=255)
                                                                                                                                cGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCg        >  2:46496/1‑140 (MQ=255)
                                                                                                                                cGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCg        >  2:302624/1‑140 (MQ=255)
                                                                                                                                cGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCg        <  1:3368952/140‑1 (MQ=255)
                                                                                                                                 gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg       >  2:906448/1‑140 (MQ=255)
                                                                                                                                 gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACCGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg       >  1:1081815/1‑140 (MQ=255)
                                                                                                                                  ggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGa      >  2:129556/1‑140 (MQ=255)
                                                                                                                                  ggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGa      >  2:1217749/1‑140 (MQ=255)
                                                                                                                                  ggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATAGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGa      >  2:253205/1‑140 (MQ=255)
                                                                                                                                    ggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGata    >  1:3185033/1‑140 (MQ=255)
                                                                                                                                    ggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGata    <  1:278764/140‑1 (MQ=255)
                                                                                                                                     gTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGGACCCGGGAAGCGGatat   >  2:870665/1‑140 (MQ=255)
                                                                                                                                      tCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATATc  >  2:826128/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                            
CGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTG‑CAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATATC  >  NC_000913/650675‑650948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑