Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,745,3970AC24.1% 288.1 / 22.9 142L198F (TTA→TTC) yiaN2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (67/40);  new base C (0/34);  total (67/75)
Fisher's exact test for biased strand distribution p-value = 4.66e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 166 total aligned reads displayed.

CTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTG  >  NC_000913/3745258‑3745536
                                                                                                                                           |                                                                                                                                           
cTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTa                                                                                                                                             >  2:615146/1‑140 (MQ=255)
   ccATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTAccc                                                                                                                                          <  2:77659/140‑1 (MQ=255)
         cGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCTGGCATGATGATGGGGGCAACGCTGATGCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTACCCCCCCac                                                                                                                                    >  2:1468507/1‑139 (MQ=255)
           tttATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCa                                                                                                                                  <  2:1557697/140‑1 (MQ=255)
           tttATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCa                                                                                                                                  >  2:2544350/1‑140 (MQ=255)
                     tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACg                                                                                                                        >  1:2341864/1‑140 (MQ=255)
                     tCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCCCCCGCAAAAaacaaca                                                                                                                        >  2:1845/1‑139 (MQ=255)
                       ggCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGAt                                                                                                                      >  2:2326955/1‑140 (MQ=255)
                            ttCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAgg                                                                                                                 <  2:115431/140‑1 (MQ=255)
                            ttCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGCCAGTCGCCCTAATTTACCCCCCCCGCAAAAAGCAAAGATGCAgg                                                                                                                 >  2:788909/1‑140 (MQ=255)
                                    gATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTgg                                                                                                         <  1:1581589/140‑1 (MQ=255)
                                       tATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCGGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAc                                                                                                      <  2:2452337/140‑1 (MQ=255)
                                       agacagtCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAc                                                                                                      <  1:846310/134‑1 (MQ=255)
                                           cATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCtt                                                                                                  <  1:2437819/140‑1 (MQ=255)
                                              cAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCttttt                                                                                               >  1:467896/1‑139 (MQ=255)
                                               aGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGt                                                                                              >  2:124218/1‑140 (MQ=255)
                                                 tAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGtct                                                                                            >  1:2144075/1‑140 (MQ=255)
                                                 tAAGCTGTTTATGGCGGGCATTGCCCCCGGCCTGATGATTGGCGCAACGCTGATGCTTTCCTTGTGGTGGCAGGCCCGTCGCCTTAATTTTCCCCGCCCGCAAAAAACAAAAATGCAGGGAATCTGGCACTCtttttttt                                                                                            >  2:2868094/1‑136 (MQ=255)
                                                   aGCTGTTTATGGCGGGCATTTCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGGGGGGGCAGGCCAGTCGCCTTAATTTACCCCCCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTcc                                                                                          >  1:1370877/1‑140 (MQ=255)
                                                   aGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGGGCAACGCTGATGCTTACCCGGGGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAACAACGATGCAGGAAAACTGGCACTCTTTTGTCTcc                                                                                          >  2:3477916/1‑140 (MQ=255)
                                                   aGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGt                                                                                              <  2:3366638/136‑1 (MQ=255)
                                                   aGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTcc                                                                                          >  1:562273/1‑140 (MQ=255)
                                                   aGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGt                                                                                              >  1:3366638/1‑136 (MQ=255)
                                                        tttATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTAt                                                                                     <  1:124218/140‑1 (MQ=255)
                                                        tttATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGGGGCAGGCCAGTCGCCTTAATTTACCCCCCCAGCAAAAAGCAACGATGCAGGGAATCTGGCACTCTTTTTTCTcccggat                                                                                     >  2:689622/1‑135 (MQ=255)
                                                        tttATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGGGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTAt                                                                                     >  1:854059/1‑140 (MQ=255)
                                                        tttATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGGGGGGGGAGGCCAGGCGCCTTAATTTACCCCCCCAGCAAAAAACAACGATGCAGGAAAACTGGCACTCTTTTTTCTCCTGtct                                                                                     >  2:846310/1‑138 (MQ=255)
                                                        tttATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGGGGGGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTAt                                                                                     >  2:1996412/1‑140 (MQ=255)
                                                           aTGGCGGGCTTTGCCCCTGGAATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTATTTTGTCTCCGGTATCTg                                                                                  <  1:2271038/140‑1 (MQ=255)
                                                               cGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                              <  1:2937565/140‑1 (MQ=255)
                                                               cGGCCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgcg                                                                              <  1:1335984/140‑1 (MQ=255)
                                                                 ggCATTGCCCTGGGCGTGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTTCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCt                                                                            <  1:2794641/140‑1 (MQ=255)
                                                                 ggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCGGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCTCTCTTTTGTCTCCGGTATCTGGGCGCt                                                                            <  1:3215753/140‑1 (MQ=255)
                                                                  gCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTTATTTACCCCGCCCGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTTTCTCCGGTATCTGGGCGCTg                                                                           >  2:1514020/1‑140 (MQ=255)
                                                                  gCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGGGGGGGCAGGCCAGTCGCCTTAATTTACCCCCCCAGCAAAAAGCAACGATGCAGGGAATCTGGGACTCTTTTTTCTCCGGTATCTGGGCGCTg                                                                           >  2:1110143/1‑140 (MQ=255)
                                                                         cccGGGCATGATGAGGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                    <  2:1370877/140‑1 (MQ=255)
                                                                          ccTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTcc                                                                   >  2:882870/1‑140 (MQ=255)
                                                                           cTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                  <  2:562273/140‑1 (MQ=255)
                                                                            tGGCATGATGATGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCGGGCCAGTCGCCTTATTTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAg                                                                 <  1:179174/140‑1 (MQ=255)
                                                                             ggCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                >  2:1682370/1‑140 (MQ=255)
                                                                              gCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCTTTATTTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                               <  2:2639385/140‑1 (MQ=255)
                                                                              gCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                               <  1:1845/140‑1 (MQ=255)
                                                                                  gatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                    >  1:402519/1‑131 (MQ=255)
                                                                                  gatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                    <  2:402519/131‑1 (MQ=255)
                                                                                   atgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                  >  2:3048356/1‑132 (MQ=255)
                                                                                   atgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                          >  2:3098105/1‑140 (MQ=255)
                                                                                   atgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                          >  2:3299733/1‑140 (MQ=255)
                                                                                   atgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                          >  1:1580060/1‑140 (MQ=255)
                                                                                   atgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCCGTTTCTTCCAGTcatcat                                                          >  2:3253107/1‑140 (MQ=255)
                                                                                    tgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatt                                                         >  1:3219975/1‑140 (MQ=255)
                                                                                     gatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatta                                                        <  2:1325945/140‑1 (MQ=255)
                                                                                      atgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                       <  1:1110143/140‑1 (MQ=255)
                                                                                      atgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                       <  1:1682370/140‑1 (MQ=255)
                                                                                        gggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                     >  1:1989835/1‑140 (MQ=255)
                                                                                        gggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                     >  2:156807/1‑140 (MQ=255)
                                                                                        gggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                     >  1:2294405/1‑140 (MQ=255)
                                                                                           cgcAACGCTGATGCTTACCTGGTGGTGGCAGCCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCg                                                  <  1:2698047/140‑1 (MQ=255)
                                                                                             cAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                >  2:3384955/1‑140 (MQ=255)
                                                                                             cAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                >  1:2605999/1‑140 (MQ=255)
                                                                                             cAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGAAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                >  2:2863747/1‑140 (MQ=255)
                                                                                               aCGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGtt                                              <  1:593974/140‑1 (MQ=255)
                                                                                                cGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGttt                                             >  2:889340/1‑140 (MQ=255)
                                                                                                cGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGttt                                             >  1:2570030/1‑140 (MQ=255)
                                                                                                    gATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCt                                         <  2:1638111/140‑1 (MQ=255)
                                                                                                    gATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCt                                         <  2:384160/140‑1 (MQ=255)
                                                                                                     aTGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTtctctc                                        >  2:975857/1‑140 (MQ=255)
                                                                                                      tGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTcc                                       <  1:1783775/140‑1 (MQ=255)
                                                                                                        cTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCgg                                     <  1:382025/140‑1 (MQ=255)
                                                                                                        cTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCgg                                     <  2:3008781/140‑1 (MQ=255)
                                                                                                         ttcccTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                    <  2:2057803/137‑1 (MQ=255)
                                                                                                         ttACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                    <  2:2341864/140‑1 (MQ=255)
                                                                                                         ttACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                    <  2:2570030/140‑1 (MQ=255)
                                                                                                         ttACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                    <  1:1468507/140‑1 (MQ=255)
                                                                                                          tACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGcc                                   >  2:209610/1‑140 (MQ=255)
                                                                                                          tACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCCGcc                                   >  2:214346/1‑140 (MQ=255)
                                                                                                              tggtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAGTCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGtt                               <  2:1989835/140‑1 (MQ=255)
                                                                                                              tggtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGtt                               <  1:3299733/140‑1 (MQ=255)
                                                                                                              tggtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGtt                               <  1:689622/140‑1 (MQ=255)
                                                                                                              tggtggtggCAGGCCAGCCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGtt                               <  1:289204/140‑1 (MQ=255)
                                                                                                              tgggggtggAGGCCCAGCCCCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGtt                               <  1:3039981/140‑1 (MQ=255)
                                                                                                               ggtggtggCAGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGAAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGttt                              <  1:2863747/140‑1 (MQ=255)
                                                                                                               ggtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGttt                              <  1:3384955/140‑1 (MQ=255)
                                                                                                                gtggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTTCGTCCTGtttt                             >  2:2165487/1‑139 (MQ=255)
                                                                                                                 tggtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCCGCCCGttttc                            >  2:58202/1‑138 (MQ=255)
                                                                                                                   gtggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGTCTTCGGGCCCGCCTGATTTccc                          >  1:1077391/1‑140 (MQ=255)
                                                                                                                    tggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGGTCCGGCCTGGTTAcccc                         >  1:2511642/1‑140 (MQ=255)
                                                                                                                    tggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCg                                      >  1:2800038/1‑127 (MQ=255)
                                                                                                                    tggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCg                                      <  2:2800038/127‑1 (MQ=255)
                                                                                                                     ggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                    <  1:2626608/128‑1 (MQ=255)
                                                                                                                     ggCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGc                                    >  2:2626608/1‑128 (MQ=255)
                                                                                                                        gggCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTAcc                     <  2:326035/139‑1 (MQ=255)
                                                                                                                        aGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTAcc                     <  1:625666/140‑1 (MQ=255)
                                                                                                                        aGGCCAGTCGCCTTAATTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTAcc                     <  1:1996412/140‑1 (MQ=255)
                                                                                                                             aGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGc                <  1:1206068/140‑1 (MQ=255)
                                                                                                                              gTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCTGCCTGTTTTTCCCTACCCAAGcc               >  2:1438402/1‑140 (MQ=255)
                                                                                                                                cGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCgg             <  2:3219975/140‑1 (MQ=255)
                                                                                                                                    ttAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCCGCCTGTTTTCCCCCACCGCAGCCgggggc         >  2:1743133/1‑138 (MQ=255)
                                                                                                                                     tAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCg        <  1:1185284/140‑1 (MQ=255)
                                                                                                                                        tttACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtg     <  1:889340/140‑1 (MQ=255)
                                                                                                                                           aCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCCGCCTGTTTTCCCCTACCGAAGCCCGGGGCGTtgctg  >  2:2634920/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTG  >  NC_000913/3745258‑3745536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑