BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,746,518	A→G	Y142C (TAC→TGC)	yiaO →	2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,746,518	0	A	G	100.0%	644.2 / NA	178	Y142C (TAC→TGC)	yiaO	2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (102/76); total (102/76)

Only 100 of 180 total aligned reads displayed.

GTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCG  >  NC_000913/3746391‑3746654

gTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACgg                                                                                                                              >  2:2863836/1‑140 (MQ=255)

gTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACgg                                                                                                                              >  1:683506/1‑140 (MQ=255)

gTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACgg                                                                                                                              >  1:2467449/1‑140 (MQ=255)

gTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACgg                                                                                                                              >  1:1842111/1‑140 (MQ=255)

 tgatgaACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGc                                                                                                                             >  1:2240252/1‑140 (MQ=255)

 tgatgaACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGc                                                                                                                             >  2:507048/1‑140 (MQ=255)

 tgatgaACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGc                                                                                                                             >  2:1855988/1‑140 (MQ=255)

    tgaACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTgg                                                                                                                          >  2:2490348/1‑140 (MQ=255)

    tgaACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTgg                                                                                                                          >  2:3157529/1‑140 (MQ=255)

    tgaACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTgg                                                                                                                          >  2:3003174/1‑140 (MQ=255)

    tgaACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTgg                                                                                                                          >  2:1229475/1‑140 (MQ=255)

      aaCTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGgcg                                                                                                                        <  1:3264762/140‑1 (MQ=255)

        cttgcttgATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGcgcg                                                                                                                      >  2:1101723/1‑140 (MQ=255)

         ttgcttgATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGa                                                                                                                     <  1:3157529/140‑1 (MQ=255)

         ttgcttgATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGa                                                                                                                     <  2:1842111/140‑1 (MQ=255)

         ttgcttgATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGa                                                                                                                     <  2:2336894/140‑1 (MQ=255)

         ttgcttgATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGa                                                                                                                     <  1:1271183/140‑1 (MQ=255)

           gcttgATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGa                                                                                                                     <  1:3403589/138‑1 (MQ=255)

           gcttgATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATg                                                                                                                   >  1:2809105/1‑140 (MQ=255)

           gcttgATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATg                                                                                                                   >  2:1183033/1‑140 (MQ=255)

           gcttgATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCACATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGgc                                                                                                                         <  1:2684147/134‑1 (MQ=255)

           gcttgATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCACATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGgc                                                                                                                         >  2:2684147/1‑134 (MQ=255)

           gcttgATGTCCCTTTCCTGTACCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGa                                                                                                                     >  2:3403589/1‑138 (MQ=255)

                   tCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAAc                                                                                                           >  2:2718176/1‑140 (MQ=255)

                         tCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTcgcgc                                                                                                     <  1:2496564/140‑1 (MQ=255)

                             gTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTcgcgcg                                                                                                    >  2:665930/1‑137 (MQ=255)

                             gTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCAc                                                                                                 >  1:905600/1‑140 (MQ=255)

                             gTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCAc                                                                                                 <  2:3305697/140‑1 (MQ=255)

                             gTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCAc                                                                                                 >  2:2542329/1‑140 (MQ=255)

                               tCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCg                                                                                               >  2:1584046/1‑140 (MQ=255)

                               tCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCg                                                                                               <  1:1195817/140‑1 (MQ=255)

                                ccGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCAc                                                                                                 <  2:177980/137‑1 (MQ=255)

                                  gcgATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCAc                                                                                                 >  1:1692920/1‑135 (MQ=255)

                                   cgATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTa                                                                                           >  1:1510737/1‑140 (MQ=255)

                                   cgATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTa                                                                                           >  2:1964935/1‑140 (MQ=255)

                                   cgATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTa                                                                                           >  1:3152821/1‑140 (MQ=255)

                                   cgATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTa                                                                                           >  2:535676/1‑140 (MQ=255)

                                     aTACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTaaa                                                                                         >  2:1011276/1‑140 (MQ=255)

                                         cGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTa                                                                                           >  2:2474566/1‑134 (MQ=255)

                                         cGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTa                                                                                           <  1:2474566/134‑1 (MQ=255)

                                           cTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAAccccc                                                                                   >  1:1116603/1‑140 (MQ=255)

                                            tCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCccg                                                                                  <  2:2205581/140‑1 (MQ=255)

                                            tCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCccg                                                                                  <  2:3153843/140‑1 (MQ=255)

                                            tCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCccg                                                                                  <  1:2751318/140‑1 (MQ=255)

                                            tCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCccg                                                                                  <  1:2346886/140‑1 (MQ=255)

                                                gcgcATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAGCTCGCGCGCACCGGTTAAAACCCCCGCCGa                                                                              >  2:2394468/1‑140 (MQ=255)

                                                gcgcATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGa                                                                              >  1:803061/1‑140 (MQ=255)

                                                     tAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGa                                                                         <  1:1011276/140‑1 (MQ=255)

                                                      aaaaCGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGaa                                                                        <  2:1510737/140‑1 (MQ=255)

                                                        aaCGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGCCTGGCGGGTTGTCACCACCTCGCGCGCACCGGTAAAAACCCCCGCCGACCTGAAAg                                                                      <  1:2294837/140‑1 (MQ=255)

                                                        aaCGCTCGACAGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAg                                                                      <  1:2852002/140‑1 (MQ=255)

                                                          cGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGa                                                                         <  2:763689/135‑1 (MQ=255)

                                                          cGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAggg                                                                    >  2:1388542/1‑140 (MQ=255)

                                                                 cGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGaaaa                                                             >  2:550967/1‑140 (MQ=255)

                                                                  ggCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGaaa                                                              <  2:2576036/138‑1 (MQ=255)

                                                                    cAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATcc                                                          <  2:3448394/140‑1 (MQ=255)

                                                                    cAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATcc                                                          <  2:1477095/140‑1 (MQ=255)

                                                                      aaGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGc                                                        <  1:1229475/140‑1 (MQ=255)

                                                                       aGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCa                                                       <  1:3003174/140‑1 (MQ=255)

                                                                        gTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAACCCCCCGCCGACCTGAAAGGGCTGAAAATCCGCAc                                                      <  2:2473951/140‑1 (MQ=255)

                                                                        gTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCAc                                                      <  1:2923211/140‑1 (MQ=255)

                                                                        gTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCAc                                                      <  1:61556/140‑1 (MQ=255)

                                                                                tgatCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATa                                              <  1:1964935/140‑1 (MQ=255)

                                                                                  atCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGc                                            <  2:1841217/140‑1 (MQ=255)

                                                                                       aaaGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGccctgt                                       >  1:2521983/1‑137 (MQ=255)

                                                                                         aGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGa                                     >  2:277867/1‑140 (MQ=255)

                                                                                            cTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTTACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAata                                  >  2:419935/1‑140 (MQ=255)

                                                                                            cTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAata                                  >  1:1497885/1‑140 (MQ=255)

                                                                                            cTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAata                                  >  2:1102991/1‑140 (MQ=255)

                                                                                             tCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAatat                                 >  1:2629676/1‑140 (MQ=255)

                                                                                              cACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATc                                >  1:2091979/1‑140 (MQ=255)

                                                                                              cACTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATc                                >  1:2797345/1‑140 (MQ=255)

                                                                                                cTTGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATcgc                              <  2:2799914/140‑1 (MQ=255)

                                                                                                  tGAAGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATcgccg                            <  2:832712/140‑1 (MQ=255)

                                                                                                    aaGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATcgccg                            >  1:1214610/1‑138 (MQ=255)

                                                                                                    aaGGTAAAGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATcgccg                            <  2:1214610/138‑1 (MQ=255)

                                                                                                         aaaGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCaa                     <  2:605820/140‑1 (MQ=255)

                                                                                                          aaGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAGCTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCaaa                    <  1:2394468/140‑1 (MQ=255)

                                                                                                           aGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAg                   >  1:254907/1‑140 (MQ=255)

                                                                                                           aGGACTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCACCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAg                   >  1:2124640/1‑140 (MQ=255)

                                                                                                              aCTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCt                >  2:2465185/1‑140 (MQ=255)

                                                                                                               cTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCtt               <  2:2091979/140‑1 (MQ=255)

                                                                                                               cTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCtt               <  2:2797345/140‑1 (MQ=255)

                                                                                                               cTGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCtt               >  1:1676144/1‑140 (MQ=255)

                                                                                                                tGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCttt              >  2:2407979/1‑140 (MQ=255)

                                                                                                                tGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCtt               >  1:413106/1‑139 (MQ=255)

                                                                                                                tGAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCtt               <  2:413106/139‑1 (MQ=255)

                                                                                                                 gAAAGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTTAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTg             >  1:3464859/1‑140 (MQ=255)

                                                                                                                  aaaGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTgg            >  1:1076902/1‑140 (MQ=255)

                                                                                                                  aaaGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTgg            >  2:2207647/1‑140 (MQ=255)

                                                                                                                    aGTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGgcg          <  1:134485/140‑1 (MQ=255)

                                                                                                                     gTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGgcgc         <  2:2240252/140‑1 (MQ=255)

                                                                                                                     gTACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTC‑TTGGCGCt        <  1:277867/140‑1 (MQ=255)

                                                                                                                      tACTGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCt        <  1:712438/140‑1 (MQ=255)

                                                                                                                         tGGCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAAc     >  2:2874105/1‑140 (MQ=255)

                                                                                                                          ggCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAAcc    >  1:3257131/1‑140 (MQ=255)

                                                                                                                          ggCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAAcc    <  1:1801818/140‑1 (MQ=255)

                                                                                                                          ggCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAAcc    >  1:574423/1‑140 (MQ=255)

                                                                                                                           gCCTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAAccc   <  2:574423/140‑1 (MQ=255)

                                                                                                                            ccTGCTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAAcccg  >  1:2079030/1‑140 (MQ=255)

GTGATGAACTTGCTTGATGTCCCTTTCCTGTTCCGCGATACCGCTCACGCGCATAAAACGCTCGACGGCAAAGTCGGTGATGATCTGAAAGCCTCACTTGAAGGTAAAGGACTGAAAGTACTGGCCTACTGGGAAAACGGCTGGCGCGATGTCACCAACTCGCGCGCACCGGTTAAAACCCCCGCCGACCTGAAAGGGCTGAAAATCCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCGCTAACCCG  >  NC_000913/3746391‑3746654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`