Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,144,442 A→G T150A (ACG→GCG)  pflD → putative formate acetyltransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,144,4420AG100.0% 801.3 / NA 220T150A (ACG→GCG) pflDputative formate acetyltransferase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (95/125);  total (95/125)

Only 100 of 221 total aligned reads displayed.

GAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTA  >  NC_000913/4144307‑4144576
                                                                                                                                       |                                                                                                                                      
  agaagaTAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATa                                                                                                                                  <  2:1123772/140‑1 (MQ=255)
  agaagaTAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATa                                                                                                                                  <  1:759742/140‑1 (MQ=255)
     agaTAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATa                                                                                                                                  >  1:2482291/1‑137 (MQ=255)
             gTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAgggg                                                                                                                       <  1:2912674/140‑1 (MQ=255)
             gTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAgggg                                                                                                                       <  2:2095295/140‑1 (MQ=255)
             gTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAgggg                                                                                                                       <  1:274537/140‑1 (MQ=255)
              tATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCa                                                                                                                           <  2:3248314/135‑1 (MQ=255)
              tATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGc                                                                                                                      >  1:2672203/1‑140 (MQ=255)
              tATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGc                                                                                                                      >  2:1798784/1‑140 (MQ=255)
              tATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGAGc                                                                                                                      >  1:1348843/1‑140 (MQ=255)
               aTCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGca                                                                                                                     <  1:577546/140‑1 (MQ=255)
               aTCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGca                                                                                                                     <  2:2496884/140‑1 (MQ=255)
               aTCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGca                                                                                                                     <  2:1996390/140‑1 (MQ=255)
                tCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGcac                                                                                                                    >  2:368255/1‑140 (MQ=255)
                tCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGcac                                                                                                                    >  2:1455578/1‑140 (MQ=255)
                 cTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGcaca                                                                                                                   <  1:1214908/140‑1 (MQ=255)
                 cTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGcaca                                                                                                                   <  1:733233/140‑1 (MQ=255)
                   aCCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAgggg                                                                                                                       >  2:2967932/1‑134 (MQ=255)
                     cGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACTAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACattat                                                                                                               >  1:1052572/1‑140 (MQ=255)
                     cGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAgggg                                                                                                                       >  1:2459627/1‑132 (MQ=255)
                     cGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGca                                                                                                                     >  2:1502651/1‑134 (MQ=255)
                      gTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACattatt                                                                                                              >  2:2785947/1‑140 (MQ=255)
                       tGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACattatta                                                                                                             >  1:24330/1‑140 (MQ=255)
                       tGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACat                                                                                                                  >  2:1605164/1‑135 (MQ=255)
                         aaGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAgggg                                                                                                                       <  2:1761628/128‑1 (MQ=255)
                         aaGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAgggg                                                                                                                       >  1:1761628/1‑128 (MQ=255)
                         aaGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGcac                                                                                                                    <  1:2582253/131‑1 (MQ=255)
                         aaGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGcac                                                                                                                    >  2:2582253/1‑131 (MQ=255)
                         aaGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACattattatt                                                                                                           >  1:791414/1‑140 (MQ=255)
                         aaGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACattattatt                                                                                                           >  2:1007863/1‑140 (MQ=255)
                           gagTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCGCATTATTATTGa                                                                                                         <  2:3400455/140‑1 (MQ=255)
                           gagTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGa                                                                                                         <  1:43377/140‑1 (MQ=255)
                              ttgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGcac                                                                                                                    >  1:2873601/1‑126 (MQ=255)
                              ttgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGca                                                                                                                     <  1:3145698/125‑1 (MQ=255)
                              ttgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGa                                                                                                         >  2:1348546/1‑137 (MQ=255)
                              ttgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGa                                                                                                         >  2:1592591/1‑137 (MQ=255)
                              ttgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTa                                                                                                      >  2:2462785/1‑140 (MQ=255)
                              ttgttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTa                                                                                                      >  1:3388586/1‑140 (MQ=255)
                              ttgttCCCGTACTGAGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTa                                                                                                      >  2:1271455/1‑140 (MQ=255)
                                 ttCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTAccc                                                                                                   >  1:1426040/1‑140 (MQ=255)
                                     cGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGc                                                                                               <  2:139930/140‑1 (MQ=255)
                                       tACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCa                                                                                                  <  1:3090069/135‑1 (MQ=255)
                                       tACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCa                                                                                                  >  2:3090069/1‑135 (MQ=255)
                                       tACTAGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCt                                                                                             >  2:2120944/1‑140 (MQ=255)
                                        aCTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTg                                                                                            >  2:181234/1‑140 (MQ=255)
                                          tGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGtt                                                                                          <  1:1949376/140‑1 (MQ=255)
                                           gggAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTAcc                                                                                                    >  1:2274590/1‑129 (MQ=255)
                                           gggAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTAcc                                                                                                    <  2:2274590/129‑1 (MQ=255)
                                           gggAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGc                                                                                               <  2:660606/134‑1 (MQ=255)
                                           gggAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTg                                                                                         >  1:2195961/1‑140 (MQ=255)
                                           gggAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTg                                                                                         >  2:1914561/1‑140 (MQ=255)
                                           gggAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTg                                                                                         >  2:879021/1‑140 (MQ=255)
                                            ggAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGa                                                                                        >  1:2099572/1‑140 (MQ=255)
                                              aaaaaCGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAAt                                                                                      <  1:2925080/140‑1 (MQ=255)
                                                   cGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACgg                                                                                 >  1:1593316/1‑140 (MQ=255)
                                                   cGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACgg                                                                                 >  1:322338/1‑140 (MQ=255)
                                                    gTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGTACATTATTATTGATTACCCAGGCCTGTTGAATCACgg                                                                                 <  2:2244781/139‑1 (MQ=255)
                                                     ttCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGc                                                                               <  2:815525/140‑1 (MQ=255)
                                                          tGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGt                                                                          <  1:1614139/140‑1 (MQ=255)
                                                            aaaGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACGCGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGa                                                                        <  1:3041314/140‑1 (MQ=255)
                                                                aTTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCtg                                                                    <  2:949372/140‑1 (MQ=255)
                                                                  ttCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCtggt                                                                  <  2:2099572/140‑1 (MQ=255)
                                                                      tcaACGGGAAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCATGGGCTGGGTGAGCTGGTGGca                                                              <  2:1401937/140‑1 (MQ=255)
                                                                        aaCGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGcaca                                                            <  1:1808564/140‑1 (MQ=255)
                                                                        aaCGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGcaca                                                            <  2:1740767/140‑1 (MQ=255)
                                                                        aaCGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGcaca                                                            <  1:949563/140‑1 (MQ=255)
                                                                         aCGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAg                                                           <  1:3358866/140‑1 (MQ=255)
                                                                          cGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGa                                                          <  2:1649825/140‑1 (MQ=255)
                                                                          cGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGa                                                          <  2:2909294/140‑1 (MQ=255)
                                                                             gCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATgc                                                       >  2:3311402/1‑140 (MQ=255)
                                                                              cagatgacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATgca                                                      <  1:1490498/140‑1 (MQ=255)
                                                                               agatgacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATgcag                                                     <  2:2929916/140‑1 (MQ=255)
                                                                                 atgacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGa                                                          >  2:2213410/1‑133 (MQ=255)
                                                                                 atgacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGa                                                          <  1:2213410/133‑1 (MQ=255)
                                                                                 atgacagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATgcagca                                                   <  2:78189/140‑1 (MQ=255)
                                                                                    acagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCGGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACa                                                >  2:2425540/1‑140 (MQ=255)
                                                                                    acagatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACa                                                <  1:93531/140‑1 (MQ=255)
                                                                                       gatgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACa                                                >  1:835224/1‑137 (MQ=255)
                                                                                        atgaAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGt                                            >  2:1192439/1‑140 (MQ=255)
                                                                                           aaGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAg                                         >  2:2936326/1‑140 (MQ=255)
                                                                                           aaGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAg                                         >  1:1790720/1‑140 (MQ=255)
                                                                                            aGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGc                                        <  2:445230/140‑1 (MQ=255)
                                                                                             gTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCa                                       >  2:2311158/1‑140 (MQ=255)
                                                                                             gTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCa                                       >  1:1149082/1‑140 (MQ=255)
                                                                                                aaaGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGc                                        <  1:2932431/136‑1 (MQ=255)
                                                                                                  aGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATgcagca                                                   >  1:479935/1‑123 (MQ=255)
                                                                                                  aGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGc                                  <  1:1007863/140‑1 (MQ=255)
                                                                                                     cgcgACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCgg                               >  2:3164463/1‑140 (MQ=255)
                                                                                                     cgcgACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCgg                               >  2:3219177/1‑140 (MQ=255)
                                                                                                     cgcgACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCgg                               <  1:1531725/140‑1 (MQ=255)
                                                                                                       cgACCAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGgag                             >  1:1664164/1‑140 (MQ=255)
                                                                                                          ccAACACGCAGATTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAAt                          <  2:1044162/140‑1 (MQ=255)
                                                                                                                     aTTTTCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCa               >  2:2924970/1‑140 (MQ=255)
                                                                                                                       tttCAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAgg             <  1:2311158/140‑1 (MQ=255)
                                                                                                                          cAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCcg          >  1:644810/1‑140 (MQ=255)
                                                                                                                          cAGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCcg          <  2:644810/140‑1 (MQ=255)
                                                                                                                           aGCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCcgc         <  1:346909/140‑1 (MQ=255)
                                                                                                                            gCATCAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCcgcg        <  1:2120944/140‑1 (MQ=255)
                                                                                                                                cAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCgttgt    <  1:3311402/140‑1 (MQ=255)
                                                                                                                                cAACCAGGCGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCgttgt    <  1:1905275/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACAGATGAAGTAAAAGCCGCGACCAACACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGATTACCCACGCCTGTTGAATCACGGGCTGGGTGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCACTTTTATCAGGCCGCGTTGTTA  >  NC_000913/4144307‑4144576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑