BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,473,852	C→T	D68N (GAT→AAT)	bdcA ←	c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,473,852	0	C	T	100.0%	851.0 / NA	234	D68N (GAT→AAT)	bdcA	c‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (117/117); total (117/117)

Only 100 of 234 total aligned reads displayed.

GGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTAC  >  NC_000913/4473715‑4473987

ggCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTaa                                                                                                                                       >  2:93274/1‑140 (MQ=255)

    tGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGAc                                                                                                                                   >  2:2322870/1‑140 (MQ=255)

    tGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGAc                                                                                                                                   <  1:460588/140‑1 (MQ=255)

     gATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACa                                                                                                                                  <  2:3234265/140‑1 (MQ=255)

      aTAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAg                                                                                                                                 >  1:2635730/1‑140 (MQ=255)

        aaGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCg                                                                                                                               <  2:2804220/140‑1 (MQ=255)

        aaGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCg                                                                                                                               <  1:2857304/140‑1 (MQ=255)

          ggAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATCCCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGtc                                                                                                                             <  2:249572/140‑1 (MQ=255)

          ggAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAACTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGtc                                                                                                                             <  2:2599912/140‑1 (MQ=255)

           gAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGtct                                                                                                                            <  1:1073203/140‑1 (MQ=255)

           gAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGtct                                                                                                                            >  2:68888/1‑140 (MQ=255)

           gAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGtct                                                                                                                            >  2:1682772/1‑140 (MQ=255)

           gAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGtct                                                                                                                            >  1:3130484/1‑140 (MQ=255)

             gCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTctct                                                                                                                          >  2:753551/1‑140 (MQ=255)

              cATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTg                                                                                                                         >  2:888078/1‑140 (MQ=255)

               aTGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGt                                                                                                                        >  1:1029045/1‑140 (MQ=255)

                tGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGa                                                                                                                                    <  2:2832545/127‑1 (MQ=255)

                tGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCg                                                                                                                               >  1:1264723/1‑132 (MQ=255)

                tGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTc                                                                                                                       >  2:114960/1‑140 (MQ=255)

                tGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTc                                                                                                                       >  1:799366/1‑140 (MQ=255)

                  aaTATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAg                                                                                                                     <  1:2322870/140‑1 (MQ=255)

                  aaTATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAg                                                                                                                     <  2:1343683/140‑1 (MQ=255)

                  aaTATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAg                                                                                                                     <  2:2550628/140‑1 (MQ=255)

                    tatTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCa                                                                                                                   <  1:1450535/140‑1 (MQ=255)

                    tatTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCa                                                                                                                   <  1:2167036/140‑1 (MQ=255)

                    tatTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCa                                                                                                                   <  1:1892063/140‑1 (MQ=255)

                    tatTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCa                                                                                                                   <  2:2635730/140‑1 (MQ=255)

                     atTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCAc                                                                                                                  >  2:1436497/1‑140 (MQ=255)

                      ttGATTTTGAAAAGGCGATCAATATCGTCTGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACt                                                                                                                 <  1:3353750/140‑1 (MQ=255)

                      ttGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAg                                                                                                                     >  1:640461/1‑136 (MQ=255)

                      ttGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAg                                                                                                                     <  2:640461/136‑1 (MQ=255)

                      ttGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCa                                                                                                                   >  1:3246174/1‑138 (MQ=255)

                      ttGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACt                                                                                                                 <  2:1899532/140‑1 (MQ=255)

                       tGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTa                                                                                                                <  2:1397697/140‑1 (MQ=255)

                       tGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTa                                                                                                                <  1:2948129/140‑1 (MQ=255)

                       tGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTa                                                                                                                <  2:536110/140‑1 (MQ=255)

                         aTTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATc                                                                                                              <  2:1029045/140‑1 (MQ=255)

                            ttGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCtgt                                                                                                           <  1:2825164/140‑1 (MQ=255)

                              gAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCt                                                                                                             <  1:1396603/136‑1 (MQ=255)

                                aaaGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAGAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAAt                                                                                                       <  2:3123129/140‑1 (MQ=255)

                                        tCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGt                                                                                               <  2:1242159/140‑1 (MQ=255)

                                            tatCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCt                                                                                           <  1:1132199/140‑1 (MQ=255)

                                              tcgtcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATAc                                                                                                     <  2:1720061/128‑1 (MQ=255)

                                              tcgtcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATAc                                                                                                     >  1:1720061/1‑128 (MQ=255)

                                              tcgtcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCg                                                                                             <  2:2732630/136‑1 (MQ=255)

                                              tcgtcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCg                                                                                             >  1:2732630/1‑136 (MQ=255)

                                               cgtcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCa                                                                                        <  2:1600162/140‑1 (MQ=255)

                                                 tcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCt                                                                                           >  1:3380637/1‑135 (MQ=255)

                                                 tcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGt                                                                                      >  2:337003/1‑140 (MQ=255)

                                                 tcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGt                                                                                      <  1:2682553/140‑1 (MQ=255)

                                                 tcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGt                                                                                      >  1:234284/1‑140 (MQ=255)

                                                 tcgGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGt                                                                                      >  2:3450873/1‑140 (MQ=255)

                                                   ggCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAg                                                                                       <  2:406969/137‑1 (MQ=255)

                                                   ggCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGtct                                                                                    >  1:1235183/1‑140 (MQ=255)

                                                    gCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCa                                                                                        >  1:196975/1‑135 (MQ=255)

                                                    gCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGtctc                                                                                   <  1:2526559/140‑1 (MQ=255)

                                                                      tCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGc                                                                 >  2:1534376/1‑140 (MQ=255)

                                                                         ccAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGc                                                              >  1:2115866/1‑140 (MQ=255)

                                                                               aCGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCttt                                                        <  2:2074287/140‑1 (MQ=255)

                                                                                    aaTACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGAcc                                                   <  1:3472430/140‑1 (MQ=255)

                                                                                    aaTACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGAcc                                                   <  2:28925/140‑1 (MQ=255)

                                                                                       aCCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCg                                                <  2:1093178/140‑1 (MQ=255)

                                                                                           gCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCa                                                             <  2:2040051/123‑1 (MQ=255)

                                                                                              tttACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGt                                         <  2:1490988/140‑1 (MQ=255)

                                                                                              tttACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGt                                         <  1:711687/140‑1 (MQ=255)

                                                                                              tttACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGt                                         <  2:3207009/140‑1 (MQ=255)

                                                                                                tACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGa                                                     <  2:3413771/126‑1 (MQ=255)

                                                                                                tACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGa                                                     >  1:3413771/1‑126 (MQ=255)

                                                                                                 accaccAGGATATCCAATGCGCCTCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGaa                                      >  1:2043649/1‑140 (MQ=255)

                                                                                                 accaccAGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGaa                                      <  2:3386656/140‑1 (MQ=255)

                                                                                                      caGGATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGt                                         <  1:3137236/132‑1 (MQ=255)

                                                                                                         gATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACAt                              >  1:1566474/1‑140 (MQ=255)

                                                                                                         gATATCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACAt                              >  1:3022848/1‑140 (MQ=255)

                                                                                                           tatCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTg                            >  1:3106094/1‑140 (MQ=255)

                                                                                                             tCCAATGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGc                          <  2:2087140/140‑1 (MQ=255)

                                                                                                                aaTGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGaa                                      >  1:2086334/1‑125 (MQ=255)

                                                                                                                aaTGCGCCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGcccc                       >  2:2189927/1‑140 (MQ=255)

                                                                                                                   gcgcCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATc                    >  1:411673/1‑140 (MQ=255)

                                                                                                                   gcgcCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATc                    >  1:2712549/1‑140 (MQ=255)

                                                                                                                   gcgcCGCTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATc                    >  2:2964793/1‑140 (MQ=255)

                                                                                                                    cgccgcTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCg                   >  1:1035752/1‑140 (MQ=255)

                                                                                                                    cgccgcTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCg                   >  2:1462171/1‑140 (MQ=255)

                                                                                                                       cgcTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTg                >  1:2108116/1‑140 (MQ=255)

                                                                                                                       cgcTCTTACGAACGACATTAATGACAGCGTCTCTGCCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTg                >  1:1185056/1‑140 (MQ=255)

                                                                                                                        gcTCTTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGa               >  1:91532/1‑140 (MQ=255)

                                                                                                                         ctctTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGAc              >  2:3130576/1‑140 (MQ=255)

                                                                                                                          tctTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACg             >  2:314698/1‑140 (MQ=255)

                                                                                                                           ctTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGa            <  2:2115866/140‑1 (MQ=255)

                                                                                                                           ctTACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGa            >  1:3209454/1‑140 (MQ=255)

                                                                                                                             tACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGaaa          <  1:1494875/140‑1 (MQ=255)

                                                                                                                             tACGAACGACATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGaaa          <  2:3022848/140‑1 (MQ=255)

                                                                                                                              acgaacgaCATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAac         >  1:1371763/1‑140 (MQ=255)

                                                                                                                              acgaacgaCATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAac         >  2:3304463/1‑140 (MQ=255)

                                                                                                                              acgaacgaCATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAac         >  1:1638890/1‑140 (MQ=255)

                                                                                                                               cgaacgaCATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacg        <  2:2043649/140‑1 (MQ=255)

                                                                                                                               cgaacgaCATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacg        <  1:3168999/140‑1 (MQ=255)

                                                                                                                                 aacgaCATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAacgac      >  2:1266300/1‑140 (MQ=255)

                                                                                                                                   cgaCATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGa            >  1:1017167/1‑132 (MQ=255)

                                                                                                                                   cgaCATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGt    <  2:234284/140‑1 (MQ=255)

                                                                                                                                      cATTAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTAc  <  1:666769/139‑1 (MQ=255)

GGCATGATAAGGAGCATGAATATTGATTTTGAAAAGGCGATCAATATCGTCGGCATTTAATTCCAGGGCCTCGCCAAAGACGCCAATACCTGCATTTACCACCAGGATATCCAATGCGCCGCTCTTACGAACGACATCAATGACAGCGTCTCTGTCAGCACTATCTGTGAATACTGCTGTCGCTCCAGTCTCTTGTGCCAGGCGTTTAGCGGCATCTTTCGACCCCGCATAGGTGAATCGTACATTGGCCCCATCGGTGACGAAACGACGTAC  >  NC_000913/4473715‑4473987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`