BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	804,164	T→C	V221A (GTC→GCC)	ybhJ →	putative hydratase YbhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	804,164	0	T	C	99.0%	724.9 / ‑3.1	202	V221A (GTC→GCC)	ybhJ	putative hydratase YbhJ
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (98/102); total (99/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 203 total aligned reads displayed.

TGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGTCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATGAAGAAGTCCATAACTGGC  >  NC_000913/804027‑804292

tGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCa                                                                                                                                <  1:2648001/140‑1 (MQ=255)

 gACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCAt                                                                                                                               <  2:201630/140‑1 (MQ=255)

   cacCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATgg                                                                                                                             >  2:2393414/1‑140 (MQ=255)

    acCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGa                                                                                                                            <  2:2468661/140‑1 (MQ=255)

    acCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGa                                                                                                                            >  2:1174775/1‑140 (MQ=255)

     ccTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGTAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAg                                                                                                                           >  1:2040018/1‑140 (MQ=255)

     ccTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAg                                                                                                                           >  2:2182942/1‑140 (MQ=255)

     ccTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAg                                                                                                                           >  2:1638000/1‑140 (MQ=255)

     ccTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAg                                                                                                                           >  1:1992616/1‑140 (MQ=255)

     ccTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTC‑AAAACGGTTACGTCAAAAACAAAGCCATGGAGt                                                                                                                          >  2:764343/1‑140 (MQ=255)

      cTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGt                                                                                                                          >  1:2797776/1‑140 (MQ=255)

      cTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGt                                                                                                                          >  1:2381887/1‑140 (MQ=255)

        gggATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTc                                                                                                                        >  2:500570/1‑140 (MQ=255)

         ggATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCg                                                                                                                       <  1:2182942/140‑1 (MQ=255)

          gATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGt                                                                                                                      >  1:2435430/1‑140 (MQ=255)

          gATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGt                                                                                                                      >  2:1506933/1‑140 (MQ=255)

              tCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGTCATGGAGTTCGTTGGa                                                                                                                  >  2:974717/1‑140 (MQ=255)

              tCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGa                                                                                                                            <  2:736841/130‑1 (MQ=255)

              tCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGa                                                                                                                            <  1:1529966/130‑1 (MQ=255)

              tCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGa                                                                                                                  <  1:564896/140‑1 (MQ=255)

              tCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGa                                                                                                                  >  1:3054530/1‑140 (MQ=255)

              tCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGa                                                                                                                  >  2:1626785/1‑140 (MQ=255)

              tCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGa                                                                                                                  >  1:176965/1‑140 (MQ=255)

                gACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGAcc                                                                                                                >  2:1398332/1‑140 (MQ=255)

                 aCTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCg                                                                                                               >  2:3458298/1‑140 (MQ=255)

                    aTCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGc                                                                                                            >  2:2504940/1‑140 (MQ=255)

                     tCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCg                                                                                                           <  2:66160/140‑1 (MQ=255)

                     tCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCg                                                                                                           >  2:885417/1‑140 (MQ=255)

                       cGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTC‑AAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTa                                                                                                        <  1:764343/140‑1 (MQ=255)

                        gggCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAGACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTa                                                                                                        >  2:3156492/1‑140 (MQ=255)

                        gggCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTa                                                                                                        >  2:1315419/1‑140 (MQ=255)

                             tGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAgcgcg                                                                                                   <  1:2526416/140‑1 (MQ=255)

                             tGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAgcgcg                                                                                                   <  1:1626785/140‑1 (MQ=255)

                             tGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAgcgcg                                                                                                   <  2:2727383/140‑1 (MQ=255)

                             tGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAgcgcg                                                                                                   <  2:2994268/140‑1 (MQ=255)

                             tGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAgcgcg                                                                                                   <  1:2299922/140‑1 (MQ=255)

                             tGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAgcgcg                                                                                                   <  1:528048/140‑1 (MQ=255)

                               gTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAGACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGct                                                                                                 <  1:3156492/140‑1 (MQ=255)

                               gTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCg                                                                                                           >  1:165914/1‑130 (MQ=255)

                               gTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGct                                                                                                 <  1:1387379/140‑1 (MQ=255)

                               gTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGct                                                                                                 >  2:1275549/1‑140 (MQ=255)

                               gTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGct                                                                                                 >  1:209773/1‑140 (MQ=255)

                               gTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGct                                                                                                 <  2:1754406/140‑1 (MQ=255)

                               gTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGct                                                                                                 >  2:362022/1‑140 (MQ=255)

                               gTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGct                                                                                                 >  1:300272/1‑140 (MQ=255)

                               gTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGct                                                                                                 <  2:3007187/140‑1 (MQ=255)

                               gTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGct                                                                                                 >  1:3211546/1‑140 (MQ=255)

                                   cGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGctctct                                                                                             >  2:115842/1‑140 (MQ=255)

                                    ggTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTa                                                                                            <  1:1275549/140‑1 (MQ=255)

                                       gCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCg                                                                                         <  1:1315419/140‑1 (MQ=255)

                                        cATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGa                                                                                        >  1:1446610/1‑140 (MQ=255)

                                         aTCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGAt                                                                                       <  2:3211546/140‑1 (MQ=255)

                                          tCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAgcgcgc                                                                                                  <  1:943166/128‑1 (MQ=255)

                                          tCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAgcgcgc                                                                                                  >  2:943166/1‑128 (MQ=255)

                                          tCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAgcgcg                                                                                                   >  1:1010664/1‑127 (MQ=255)

                                               ccGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGt                                                                                 <  2:2874464/140‑1 (MQ=255)

                                                 ggAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTaa                                                                               <  1:2393414/140‑1 (MQ=255)

                                                   aaaaCCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACa                                                                             <  1:885417/140‑1 (MQ=255)

                                                         tGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTg                                                                       <  2:588278/140‑1 (MQ=255)

                                                         tGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTg                                                                       <  2:2797776/140‑1 (MQ=255)

                                                         tGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTg                                                                       <  2:1992616/140‑1 (MQ=255)

                                                          gcgcCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGa                                                                      >  1:992624/1‑140 (MQ=255)

                                                          gcgcCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGa                                                                      >  2:1780040/1‑140 (MQ=255)

                                                           cgcCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTg                                                                       <  1:3114094/138‑1 (MQ=255)

                                                           cgcCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTg                                                                       >  2:3114094/1‑138 (MQ=255)

                                                                tATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGtgat                                                                <  1:1174775/140‑1 (MQ=255)

                                                                tATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGtgat                                                                >  2:607702/1‑140 (MQ=255)

                                                                tATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGtgat                                                                <  2:1814950/140‑1 (MQ=255)

                                                                tATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGtgat                                                                <  2:176965/140‑1 (MQ=255)

                                                                tATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGtgat                                                                <  1:355083/140‑1 (MQ=255)

                                                                     ggggCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCa                                                           <  1:1144269/140‑1 (MQ=255)

                                                                       ggCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACt                                                         <  2:1554669/140‑1 (MQ=255)

                                                                          cacaGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCTGTAACAGCGTTGACGTGATGACCACTGaa                                                      >  2:334488/1‑140 (MQ=255)

                                                                          cacaGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGaa                                                      >  2:649105/1‑140 (MQ=255)

                                                                          cacaGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGaa                                                      >  1:2203024/1‑140 (MQ=255)

                                                                          cacaGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGaa                                                      >  1:2878151/1‑140 (MQ=255)

                                                                          cacaGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGCTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGaa                                                      >  1:664015/1‑140 (MQ=255)

                                                                           acaGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGCCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGaaa                                                     >  1:539607/1‑140 (MQ=255)

                                                                            caGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAAc                                                    >  1:1978351/1‑140 (MQ=255)

                                                                              ggATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGa                                                  <  1:1780040/140‑1 (MQ=255)

                                                                               gATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGAc                                                 <  1:628554/140‑1 (MQ=255)

                                                                               gATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGAc                                                 <  1:2988865/140‑1 (MQ=255)

                                                                                aTGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGAcc                                                <  2:2633976/140‑1 (MQ=255)

                                                                                 tgtgGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGAc                                                 <  1:2490123/138‑1 (MQ=255)

                                                                                  gtgGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTg                                              <  2:646011/140‑1 (MQ=255)

                                                                                   tgGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGt                                             >  2:2226657/1‑140 (MQ=255)

                                                                                    ggCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGtt                                            <  2:2381887/140‑1 (MQ=255)

                                                                                         tGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGt                                       >  2:673754/1‑140 (MQ=255)

                                                                                                   tGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGc                             <  1:392829/140‑1 (MQ=255)

                                                                                                     gcgcGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCaa                           >  1:3278264/1‑140 (MQ=255)

                                                                                                     gcgcGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCaa                           >  2:1076960/1‑140 (MQ=255)

                                                                                                     gcgcGGTGTTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCa                            <  2:533838/139‑1 (MQ=255)

                                                                                                          gtgtTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGa                      >  1:1162638/1‑140 (MQ=255)

                                                                                                           tgtTCAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGAt                     >  2:2559518/1‑140 (MQ=255)

                                                                                                               cAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATgaag                 <  1:2509486/140‑1 (MQ=255)

                                                                                                               cAAAAACGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATgaag                 <  2:2127200/140‑1 (MQ=255)

                                                                                                                 aaaaCGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATgaagaa               <  2:2069460/140‑1 (MQ=255)

                                                                                                                     cGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATGAAGAAGTcc           >  1:2725082/1‑140 (MQ=255)

                                                                                                                     cGGTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATGAAGAAGTcc           >  1:3413512/1‑140 (MQ=255)

                                                                                                                       gTTACGTCAAAAACAAAGCCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATgaag                 >  1:2135878/1‑132 (MQ=255)

TGACACCTGGGATATCGACTATCCGGGCGTGGTTGCGGTGCATCTGACCGGAAAACCTGCGCCGTATGTGGGGCCACAGGATGTGGCGCTGGCTATCATTGGCGCGGTGTTCAAAAACGGTTACGTCAAAAACAAAGTCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATGAAGAAGTCCATAACTGGC  >  NC_000913/804027‑804292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`