BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,092,286	A→G	Y2H (TAT→CAT)	pgaA ←	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,092,286	0	A	G	100.0%	945.3 / NA	259	Y2H (TAT→CAT)	pgaA	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine outer membrane porin
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (116/143); total (116/143)

Only 100 of 262 total aligned reads displayed.

TTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAG  >  NC_000913/1092150‑1092422

 tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                      <  2:1022170/140‑1 (MQ=255)

 tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                      <  1:2500655/140‑1 (MQ=255)

 tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                      <  2:2127918/140‑1 (MQ=255)

 tGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCACGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                      <  1:2293041/140‑1 (MQ=255)

  gCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATcc                                                                                                                                     >  2:26609/1‑140 (MQ=255)

  gCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATcc                                                                                                                                     >  1:3055934/1‑140 (MQ=255)

  gCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATcc                                                                                                                                     >  2:422068/1‑140 (MQ=255)

  gCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATcc                                                                                                                                     >  1:2997233/1‑140 (MQ=255)

      gCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa                                                                                                                                 <  2:757435/140‑1 (MQ=255)

       cTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTAt                                                                                                                                <  2:1519720/140‑1 (MQ=255)

       cTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTAt                                                                                                                                >  2:1186169/1‑140 (MQ=255)

         tCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTa                                                                                                                              <  1:1439234/140‑1 (MQ=255)

          cAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTAc                                                                                                                             >  1:6160/1‑140 (MQ=255)

           aataatCAATGCATCATAGGCGTTATTAACAGCACTCTTCGTCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACt                                                                                                                            >  1:1931431/1‑140 (MQ=255)

           aataatCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACt                                                                                                                            >  1:389646/1‑140 (MQ=255)

           aataatCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACt                                                                                                                            <  2:3370301/140‑1 (MQ=255)

                  aaTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTa                                                                                                                     >  1:2832169/1‑140 (MQ=255)

                    tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTAtt                                                                                                                               <  1:3113372/128‑1 (MQ=255)

                    tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTAtt                                                                                                                   >  2:2722369/1‑140 (MQ=255)

                    tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTAtt                                                                                                                   >  1:1525723/1‑140 (MQ=255)

                    tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTAtt                                                                                                                   >  1:2948940/1‑140 (MQ=255)

                    tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTAtt                                                                                                                   >  2:1997994/1‑140 (MQ=255)

                    tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTAtt                                                                                                                   >  1:2854483/1‑140 (MQ=255)

                    tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTAtt                                                                                                                   >  2:1724709/1‑140 (MQ=255)

                    tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTAtt                                                                                                                   >  2:126070/1‑140 (MQ=255)

                      catcatAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGc                                                                                                                 <  2:1219752/140‑1 (MQ=255)

                        tcatAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCa                                                                                                               <  1:3234173/140‑1 (MQ=255)

                         catAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCaa                                                                                                              >  2:2174224/1‑140 (MQ=255)

                             ggCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtc                                                                                                          <  1:1348693/140‑1 (MQ=255)

                               cGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctc                                                                                                        <  2:930075/140‑1 (MQ=255)

                                gTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctct                                                                                                       <  2:751717/140‑1 (MQ=255)

                                  tattaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                     <  2:1895588/140‑1 (MQ=255)

                                  tattaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                     <  1:2333335/140‑1 (MQ=255)

                                  tattaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                     <  2:3289390/140‑1 (MQ=255)

                                  tattaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                     <  1:1186169/140‑1 (MQ=255)

                                   attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCaaaa                                                                                                            >  2:2573064/1‑132 (MQ=255)

                                   attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctctc                                                                                                    <  1:2224384/140‑1 (MQ=255)

                                   attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctctc                                                                                                    >  2:817760/1‑140 (MQ=255)

                                   attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctctc                                                                                                    >  1:484017/1‑140 (MQ=255)

                                   attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtct                                                                                                         <  2:863243/135‑1 (MQ=255)

                                    ttaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATctctctct                                                                                                   <  1:1247323/140‑1 (MQ=255)

                                        cAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                               <  2:1612295/140‑1 (MQ=255)

                                          gCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                     <  2:900630/132‑1 (MQ=255)

                                          gCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                     >  1:900630/1‑132 (MQ=255)

                                          gCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                               >  2:2300801/1‑138 (MQ=255)

                                            aCTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                     <  2:3489289/130‑1 (MQ=255)

                                            aCTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctct                                                                                                       <  1:1129437/128‑1 (MQ=255)

                                            aCTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctct                                                                                                       >  2:1129437/1‑128 (MQ=255)

                                            aCTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTaa                                                                                           >  2:2881977/1‑140 (MQ=255)

                                              tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                     <  1:2077718/128‑1 (MQ=255)

                                              tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                     >  2:2077718/1‑128 (MQ=255)

                                              tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTaa                                                                                           <  1:3256970/138‑1 (MQ=255)

                                              tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTaa                                                                                           >  2:3256970/1‑138 (MQ=255)

                                              tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAAtt                                                                                         >  1:1857774/1‑140 (MQ=255)

                                              tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAAtt                                                                                         >  1:1990143/1‑140 (MQ=255)

                                              tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAAtt                                                                                         >  1:2201474/1‑140 (MQ=255)

                                              tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAAtt                                                                                         >  1:1913083/1‑140 (MQ=255)

                                              tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCACTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctct                                                                                                       <  1:2660696/126‑1 (MQ=255)

                                                ttCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTaa                                                                                       >  2:583796/1‑139 (MQ=255)

                                                ttCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTAc                                                                                       >  2:1916026/1‑140 (MQ=255)

                                                    ccgcGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGt                                                                                   <  1:817760/140‑1 (MQ=255)

                                                      gcgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTaa                                                                                 >  1:1072713/1‑140 (MQ=255)

                                                      gcgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTaa                                                                                 <  1:3340696/140‑1 (MQ=255)

                                                       cgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAAc                                                                                >  1:2572696/1‑140 (MQ=255)

                                                        gggACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                               >  1:2089002/1‑124 (MQ=255)

                                                        gggACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACt                                                                               >  1:1315617/1‑140 (MQ=255)

                                                          gACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGa                                                                             >  1:1005077/1‑140 (MQ=255)

                                                            cTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGaaa                                                                           <  1:1610932/140‑1 (MQ=255)

                                                                ctgctAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAaaagaa                                                                       <  1:412158/140‑1 (MQ=255)

                                                                        aaTGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATAttt                                                               <  2:2033890/140‑1 (MQ=255)

                                                                           gCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTg                                                            <  2:2825903/140‑1 (MQ=255)

                                                                           gCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTg                                                            <  1:1724709/140‑1 (MQ=255)

                                                                            cGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGc                                                           <  2:2201474/140‑1 (MQ=255)

                                                                            cGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGc                                                           <  2:3403786/140‑1 (MQ=255)

                                                                             ggCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCa                                                          <  1:126053/140‑1 (MQ=255)

                                                                             ggCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCa                                                          >  1:2170911/1‑140 (MQ=255)

                                                                              gCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTg                                                            <  1:1052810/137‑1 (MQ=255)

                                                                              gCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTg                                                            >  2:1052810/1‑137 (MQ=255)

                                                                              gCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCAc                                                         <  1:2722369/140‑1 (MQ=255)

                                                                               cAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCAcc                                                        <  1:907013/140‑1 (MQ=255)

                                                                                aGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGc                                                           >  2:503126/1‑136 (MQ=255)

                                                                                  tAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCa                                                     <  2:2997233/140‑1 (MQ=255)

                                                                                  tAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCa                                                     <  2:1387385/140‑1 (MQ=255)

                                                                                    agaagTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATa                                                   <  2:1857774/140‑1 (MQ=255)

                                                                                      aagTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAAt                                                 <  2:2938986/140‑1 (MQ=255)

                                                                                      aagTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAAt                                                 <  1:51242/140‑1 (MQ=255)

                                                                                         tttCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCg                                              >  2:3003445/1‑140 (MQ=255)

                                                                                           tCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGtt                                            >  2:3104827/1‑140 (MQ=255)

                                                                                           tCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGtt                                            >  1:2763131/1‑140 (MQ=255)

                                                                                               aGCCCATTTGGGTTTCGGGCACCTTTTTCTGCCAATTGAATTCAATCTTTATTAACTCATGGAATTCCAAAATTTCTTCCTGGATTTTATTACCGGGAACTTGAAAGGAAGGTATTTTTGCAACCCATAATTCGgtatta                                        >  2:386653/1‑140 (MQ=255)

                                                                                                  ccATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAAc                                     <  2:1362505/140‑1 (MQ=255)

                                                                                                   cATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAAcg                                    <  2:2457645/140‑1 (MQ=255)

                                                                                                   cATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAAcg                                    <  1:309021/140‑1 (MQ=255)

                                                                                                   cATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCACCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAAcg                                    >  1:1884542/1‑140 (MQ=255)

                                                                                                     tttGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAAcgcg                                  >  1:2610892/1‑140 (MQ=255)

                                                                                                               gggCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTaa                                       <  1:3460448/125‑1 (MQ=255)

                                                                                                               gggCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGAc                        >  1:368393/1‑140 (MQ=255)

                                                                                                                              cTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTtgatga         >  2:609152/1‑140 (MQ=255)

                                                                                                                                   tGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATgaga    <  2:1315617/140‑1 (MQ=255)

                                                                                                                                   tGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATgaga    <  1:2405455/140‑1 (MQ=255)

TTGCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGAAG  >  NC_000913/1092150‑1092422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`