BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	60,088	A→G	F87L (TTC→CTC)	rluA ←	23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	60,088	0	A	G	100.0%	605.6 / NA	174	F87L (TTC→CTC)	rluA	23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (87/87); total (87/87)

Only 100 of 179 total aligned reads displayed.

CCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTG  >  NC_000913/59952‑60223

     ttCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGtt                                                                                                                                 >  1:29658/1‑140 (MQ=255)

     ttCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGtt                                                                                                                                 >  2:1271013/1‑140 (MQ=255)

     ttCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGtt                                                                                                                                 >  2:566211/1‑140 (MQ=255)

     ttCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGtt                                                                                                                                 >  1:1480454/1‑140 (MQ=255)

     ttCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGtt                                                                                                                                 >  2:38827/1‑140 (MQ=255)

     ttCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGtt                                                                                                                                 >  2:2074/1‑140 (MQ=255)

     ttCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGtt                                                                                                                                 >  2:1579008/1‑140 (MQ=255)

     ttCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCAGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGtt                                                                                                                                 >  2:2274216/1‑140 (MQ=255)

       cGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGtttt                                                                                                                               <  2:1442981/140‑1 (MQ=255)

          aaCAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAAc                                                                                                                            <  2:241391/140‑1 (MQ=255)

                   tCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTcc                                                                                                                   >  2:326431/1‑140 (MQ=255)

                    cTGTTTCTGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCcg                                                                                                                  <  2:991949/140‑1 (MQ=255)

                    cTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCACCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCcg                                                                                                                  <  1:2639548/140‑1 (MQ=255)

                         tCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCt                                                                                                             <  2:2318411/140‑1 (MQ=255)

                          cGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCtt                                                                                                            >  1:277740/1‑140 (MQ=255)

                           gggCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCttt                                                                                                           >  1:1530932/1‑140 (MQ=255)

                           gggCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGGTTTAACTCCCGCTCCGCGGCttt                                                                                                           >  2:1786734/1‑140 (MQ=255)

                              cGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGt                                                                                                        <  1:635890/140‑1 (MQ=255)

                              cGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGt                                                                                                        <  1:301249/140‑1 (MQ=255)

                                 tttGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCcg                                                                                                                  >  2:307813/1‑127 (MQ=255)

                                 tttGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCcg                                                                                                                  >  2:2975295/1‑127 (MQ=255)

                                 tttGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCcg                                                                                                                  <  1:2975295/127‑1 (MQ=255)

                                 tttGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAg                                                                                                     >  2:2096278/1‑140 (MQ=255)

                                 tttGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAg                                                                                                     <  1:2096278/140‑1 (MQ=255)

                                  ttGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCAGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAgc                                                                                                    <  1:2274216/140‑1 (MQ=255)

                                   tGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAgcg                                                                                                   <  1:350773/140‑1 (MQ=255)

                                   tGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAgcg                                                                                                   <  1:3122679/140‑1 (MQ=255)

                                   tGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAgcg                                                                                                   <  1:1959922/140‑1 (MQ=255)

                                       cAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGTCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCAc                                                                                               <  1:826908/140‑1 (MQ=255)

                                         gTCGCAAATGAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGGGCCACTa                                                                                             <  2:2958623/140‑1 (MQ=255)

                                         gTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAgc                                                                                                    <  2:749847/133‑1 (MQ=255)

                                         gTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAgc                                                                                                    >  1:749847/1‑133 (MQ=255)

                                         gTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTa                                                                                             >  2:3147253/1‑140 (MQ=255)

                                          tCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTAc                                                                                            >  2:2799192/1‑140 (MQ=255)

                                          tCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTAc                                                                                            <  1:2074/140‑1 (MQ=255)

                                          tCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTAc                                                                                            >  2:3358211/1‑140 (MQ=255)

                                          tCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTAc                                                                                            >  2:510010/1‑140 (MQ=255)

                                          tCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTAc                                                                                            >  1:1344730/1‑140 (MQ=255)

                                                    gCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCAcgccg                                                                                  <  1:2326971/140‑1 (MQ=255)

                                                    gCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCAcgccg                                                                                  >  1:3356204/1‑140 (MQ=255)

                                                    gCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCAcgccg                                                                                  <  1:400682/140‑1 (MQ=255)

                                                     cGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCAcgc                                                                                    >  1:1971521/1‑137 (MQ=255)

                                                        cAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTgg                                                                              <  2:2033791/140‑1 (MQ=255)

                                                        cAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTgg                                                                              <  2:826611/140‑1 (MQ=255)

                                                          gATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTgg                                                                              >  2:1317399/1‑138 (MQ=255)

                                                          gATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTgg                                                                              <  1:1317399/138‑1 (MQ=255)

                                                           aTCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAg                                                                           <  2:1854978/140‑1 (MQ=255)

                                                           aTCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTAGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAg                                                                           <  2:912557/140‑1 (MQ=255)

                                                             ccaccaGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGcc                                                                         <  2:2896970/140‑1 (MQ=255)

                                                                  aGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATc                                                                    <  1:1351116/140‑1 (MQ=255)

                                                                   gACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATcc                                                                   >  2:804084/1‑140 (MQ=255)

                                                                    aCCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCa                                                                  <  2:3267125/140‑1 (MQ=255)

                                                                    aCCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCa                                                                  >  2:846284/1‑140 (MQ=255)

                                                                    aCCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCa                                                                  >  1:119220/1‑140 (MQ=255)

                                                                    aCCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCa                                                                  >  2:1933198/1‑140 (MQ=255)

                                                                     ccTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGcc                                                                         >  1:2461879/1‑132 (MQ=255)

                                                                     ccTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGcc                                                                         <  2:2461879/132‑1 (MQ=255)

                                                                     ccTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAg                                                                 >  2:212481/1‑140 (MQ=255)

                                                                     ccTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAg                                                                 >  1:2295304/1‑140 (MQ=255)

                                                                      cTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGa                                                                >  1:702762/1‑140 (MQ=255)

                                                                      cTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGa                                                                >  1:3099194/1‑140 (MQ=255)

                                                                      cTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGa                                                                <  2:1393579/140‑1 (MQ=255)

                                                                        tCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGc                                                                          <  1:2823997/128‑1 (MQ=255)

                                                                        tCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACg                                                              >  2:2520035/1‑140 (MQ=255)

                                                                         cTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGa                                                             >  1:3367587/1‑140 (MQ=255)

                                                                         cTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGa                                                             <  2:3354937/140‑1 (MQ=255)

                                                                         cTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGa                                                             <  1:1933198/140‑1 (MQ=255)

                                                                         cTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGa                                                             >  1:1414136/1‑140 (MQ=255)

                                                                          tGCGGGGGATGGATGCCCCCAGACGCGGGCCGCATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGAt                                                            >  2:1519856/1‑140 (MQ=255)

                                                                          tGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGAt                                                            >  1:1094503/1‑140 (MQ=255)

                                                                                   tggatgCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGAtt                                                   <  1:151786/140‑1 (MQ=255)

                                                                                       tgCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTctgc                                               >  1:32671/1‑140 (MQ=255)

                                                                                       tgCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTctgc                                               <  2:702762/140‑1 (MQ=255)

                                                                                       tgCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTctgc                                               <  1:3195001/140‑1 (MQ=255)

                                                                                          ccccAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAACCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTctgcctg                                            <  2:1079432/140‑1 (MQ=255)

                                                                                            ccAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCg                                          <  2:481456/140‑1 (MQ=255)

                                                                                                  gcgGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAAt                                    <  2:2755074/140‑1 (MQ=255)

                                                                                                          caTACTGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGa                            >  1:1675343/1‑140 (MQ=255)

                                                                                                               tGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATgcg                       <  2:1414136/140‑1 (MQ=255)

                                                                                                               tGCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATgcg                       <  2:2838160/140‑1 (MQ=255)

                                                                                                                gCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGa                            <  1:1852583/134‑1 (MQ=255)

                                                                                                                gCTTTTTCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATgcgc                      >  2:1388419/1‑140 (MQ=255)

                                                                                                                 gtttttCGGCTCGCGCTCGGGGATCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGcgcg                     <  2:1948494/139‑1 (MQ=255)

                                                                                                                   ttttCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGtc                   >  2:502902/1‑140 (MQ=255)

                                                                                                                    tttCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACCCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGtca                  >  1:2600422/1‑140 (MQ=255)

                                                                                                                     ttCGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGtcat                 <  2:3367587/140‑1 (MQ=255)

                                                                                                                       cGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGtcatca               >  2:3171407/1‑140 (MQ=255)

                                                                                                                       cGGCTCGCGCTCGCGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGtcatca               >  2:1551517/1‑140 (MQ=255)

                                                                                                                         gctcgcgctcgcGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACg             >  1:932256/1‑140 (MQ=255)

                                                                                                                         gctcgcgctcgcGGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACg             <  1:3171407/140‑1 (MQ=255)

                                                                                                                          ctcgcgctcgcgGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGc            <  1:510010/140‑1 (MQ=255)

                                                                                                                           tcgcgctcgcgGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAACCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCt           <  2:338917/140‑1 (MQ=255)

                                                                                                                              cgctcgcgGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGc            >  1:1714302/1‑136 (MQ=255)

                                                                                                                               gctcgcgGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCt       <  1:846284/140‑1 (MQ=255)

                                                                                                                                ctcgcgGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCtt      >  1:2865228/1‑140 (MQ=255)

                                                                                                                                  cgcgGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTtg    >  1:112586/1‑140 (MQ=255)

                                                                                                                                    cgGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTtgtg  <  1:2520035/140‑1 (MQ=255)

                                                                                                                                    cgGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTtgtg  <  2:1675343/140‑1 (MQ=255)

                                                                                                                                    cgGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTtgtg  <  2:1344730/140‑1 (MQ=255)

                                                                                                                                    cgGAGCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTtgtg  <  2:1094503/140‑1 (MQ=255)

CCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTG  >  NC_000913/59952‑60223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`