BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	113,148	A→T	D24E (GAT→GAA)	coaE ←	dephospho‑CoA kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	113,148	0	A	T	99.1%	765.8 / ‑3.1	219	D24E (GAT→GAA)	coaE	dephospho‑CoA kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (100/117); minor base C (1/1); total (101/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01

Only 100 of 221 total aligned reads displayed.

CCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGTTAAAAATGAGTGCACGAAAGCGAAA  >  NC_000913/113015‑113284

cccGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCa                                                                                                                                    <  2:1244815/140‑1 (MQ=255)

  cGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCaaa                                                                                                                                  <  1:2304484/140‑1 (MQ=255)

   ggCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAAc                                                                                                                                 >  1:2554990/1‑140 (MQ=255)

     cgcTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGc                                                                                                                               <  2:12153/140‑1 (MQ=255)

      gcTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCa                                                                                                                              <  2:3025222/140‑1 (MQ=255)

         gCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTg                                                                                                                           >  2:1754920/1‑140 (MQ=255)

          cAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTgg                                                                                                                          <  1:1872813/140‑1 (MQ=255)

          cAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTgg                                                                                                                          <  2:1517722/140‑1 (MQ=255)

                 ccATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCAtt                                                                                                                            >  1:1773242/1‑131 (MQ=255)

                  cATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGc                                                                                                                               <  1:2952940/127‑1 (MQ=255)

                  cATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTa                                                                                                                  >  1:752039/1‑140 (MQ=255)

                    tCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTAct                                                                                                                >  1:735634/1‑140 (MQ=255)

                    tCAGCAGCAATCATGTTAGCGCAAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTAct                                                                                                                <  2:1544209/140‑1 (MQ=255)

                     cagcagCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTgg                                                                                                                          >  2:2582928/1‑129 (MQ=255)

                     cagcagCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTgg                                                                                                                          <  1:2582928/129‑1 (MQ=255)

                     cagcagCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTActc                                                                                                               <  2:682568/140‑1 (MQ=255)

                        cagcaATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCa                                                                                                                              >  1:1044440/1‑122 (MQ=255)

                           caATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCa                                                                                                         >  1:181562/1‑140 (MQ=255)

                           caATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCa                                                                                                         <  2:91630/140‑1 (MQ=255)

                              tCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTg                                                                                                      <  1:122697/140‑1 (MQ=255)

                                     aGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGCTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGc                                                                                               >  2:2418351/1‑140 (MQ=255)

                                      gcgcCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGcc                                                                                              >  2:1275674/1‑140 (MQ=255)

                                      gcgcCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGcc                                                                                              >  2:1980793/1‑140 (MQ=255)

                                      gcgcCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGcc                                                                                              >  2:3468794/1‑140 (MQ=255)

                                      gcgcCAAAGTGATCAGCAATGGCATGTAGCGCAGGCGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGcc                                                                                              >  2:1105768/1‑140 (MQ=255)

                                        gcCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGcc                                                                                                          <  2:2944995/126‑1 (MQ=255)

                                        gcCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTc                                                                                            >  2:2775046/1‑140 (MQ=255)

                                        gcCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTc                                                                                            >  1:973397/1‑140 (MQ=255)

                                          cAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTccc                                                                                          >  1:2927171/1‑140 (MQ=255)

                                          cAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGCTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTccc                                                                                          <  1:2418351/140‑1 (MQ=255)

                                           aaaGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCg                                                                                         >  1:3431637/1‑140 (MQ=255)

                                             aGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGtt                                                                                       <  1:126393/140‑1 (MQ=255)

                                             aGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGtt                                                                                       <  2:861498/140‑1 (MQ=255)

                                               tGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTaa                                                                                     <  2:812121/140‑1 (MQ=255)

                                                 aTCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGTCATCAATGACGTTAATTCCGAGTTCAGCAACCGCATTGGCAACGTTACTCTTGCCGCTGCCCATGTTTCGCGTTAAgg                                                                                   <  2:223901/140‑1 (MQ=255)

                                                 aTCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCTACTGCCAATGCCTCCCGTTAAgg                                                                                   <  2:3137123/140‑1 (MQ=255)

                                                 aTCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAgg                                                                                   <  1:465514/140‑1 (MQ=255)

                                                 aTCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAgg                                                                                   <  1:2775046/140‑1 (MQ=255)

                                                 aTCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAgg                                                                                   <  2:226382/140‑1 (MQ=255)

                                                 aTCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAgg                                                                                   <  1:1420596/140‑1 (MQ=255)

                                                 aTCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAgg                                                                                   <  1:31133/140‑1 (MQ=255)

                                                  tCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGc                                                                                  <  2:2225166/140‑1 (MQ=255)

                                                  tCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGc                                                                                  <  2:2072173/140‑1 (MQ=255)

                                                  tCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGc                                                                                  <  1:693481/140‑1 (MQ=255)

                                                  tCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGc                                                                                  <  2:1689128/140‑1 (MQ=255)

                                                  tCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGc                                                                                  <  1:2215984/140‑1 (MQ=255)

                                                  tCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGc                                                                                  <  2:1169767/140‑1 (MQ=255)

                                                   cAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCa                                                                                 <  1:516074/140‑1 (MQ=255)

                                                    aGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCaa                                                                                <  2:181562/140‑1 (MQ=255)

                                                      cAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCTACTGCCAATGCCTCCCGTTAAGGCAACt                                                                              >  1:1390738/1‑140 (MQ=255)

                                                         tGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAgg                                                                                   <  1:800478/132‑1 (MQ=255)

                                                         tGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGc                                                                                  <  1:1792743/133‑1 (MQ=255)

                                                          ggCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACtatat                                                                          <  2:3431637/140‑1 (MQ=255)

                                                           gCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCACAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACtatata                                                                         >  2:70539/1‑140 (MQ=255)

                                                              tGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCt                                                                      <  1:213290/140‑1 (MQ=255)

                                                                tAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGc                                                                                  >  2:3351508/1‑126 (MQ=255)

                                                                tAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGc                                                                                  >  2:2975413/1‑126 (MQ=255)

                                                                tAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGc                                                                                  <  1:3351508/126‑1 (MQ=255)

                                                                   cgcAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATaa                                                                 >  1:1894827/1‑140 (MQ=255)

                                                                     cAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAAtt                                                               >  1:1545642/1‑140 (MQ=255)

                                                                       ggTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTta                                                             >  1:2760667/1‑140 (MQ=255)

                                                                        gTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACtata                                                                           <  2:557566/125‑1 (MQ=255)

                                                                        gTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACtata                                                                           >  1:557566/1‑125 (MQ=255)

                                                                        gTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACATTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTtat                                                            >  2:2286554/1‑140 (MQ=255)

                                                                         tGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTtata                                                           <  2:3196822/140‑1 (MQ=255)

                                                                         tGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTtata                                                           >  2:102771/1‑140 (MQ=255)

                                                                          gCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTatat                                                          >  1:3252338/1‑140 (MQ=255)

                                                                           cACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTtata                                                           <  2:3292875/138‑1 (MQ=255)

                                                                           cACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTtata                                                           >  1:3292875/1‑138 (MQ=255)

                                                                           cACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATAtt                                                         <  2:2807358/140‑1 (MQ=255)

                                                                             ccTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTcc                                                       >  1:1165011/1‑140 (MQ=255)

                                                                              cTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTccc                                                      <  2:2365391/140‑1 (MQ=255)

                                                                               tGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCg                                                     <  1:319080/140‑1 (MQ=255)

                                                                                    cAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAAt                                                <  1:2986311/140‑1 (MQ=255)

                                                                                      accaccTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATtc                                              >  2:2458600/1‑140 (MQ=255)

                                                                                        caccTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATtcat                                            <  2:1422803/140‑1 (MQ=255)

                                                                                         accTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATtcatc                                           <  1:225251/140‑1 (MQ=255)

                                                                                           cTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTcc                                                       >  2:152716/1‑126 (MQ=255)

                                                                                           cTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATtca                                             >  1:2203255/1‑136 (MQ=255)

                                                                                           cTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATtca                                             <  2:2203255/136‑1 (MQ=255)

                                                                                           cTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTcatcat                                         >  1:1519319/1‑140 (MQ=255)

                                                                                           cTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTcatcat                                         >  2:1845645/1‑140 (MQ=255)

                                                                                                    aataatATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCaa                                <  2:1894827/140‑1 (MQ=255)

                                                                                                    aataatATCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCaa                                <  1:1105768/140‑1 (MQ=255)

                                                                                                     ataataTCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCaaa                               >  1:2513008/1‑140 (MQ=255)

                                                                                                      taataTCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCaaaa                              <  1:870765/140‑1 (MQ=255)

                                                                                                        atatCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACg                            <  1:102771/140‑1 (MQ=255)

                                                                                                        atatCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACg                            <  1:1434114/140‑1 (MQ=255)

                                                                                                          atCGGCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGtt                          <  1:1348355/140‑1 (MQ=255)

                                                                                                              gCATCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGTTaaaa                      >  1:3059133/1‑140 (MQ=255)

                                                                                                                aTCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGTTAAAAAt                    <  2:414236/140‑1 (MQ=255)

                                                                                                                aTCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGTTAAAAAt                    <  1:70539/140‑1 (MQ=255)

                                                                                                                aTCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGTTAAAAAt                    >  2:1785299/1‑140 (MQ=255)

                                                                                                                aTCAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGTTAAAAAt                    >  1:1923085/1‑140 (MQ=255)

                                                                                                                  cAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGTTAAAAATGa                  <  2:3124290/140‑1 (MQ=255)

                                                                                                                  cAATGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGTTAAAAATGa                  >  1:882480/1‑140 (MQ=255)

                                                                                                                   aaTGACGTTAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCaa                                >  2:970407/1‑125 (MQ=255)

                                                                                                                          ttAATTCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGTTAAAAATGAGTGCACGa          >  1:382080/1‑140 (MQ=255)

                                                                                                                              ttCCGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGTTAAAAATGa                  >  2:774938/1‑128 (MQ=255)

                                                                                                                                ccGAGTTCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATTATTATCAAAACGTTAAAAATGAGTGCACGAAAGCGa    <  2:255062/140‑1 (MQ=255)

CCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATTATCAAAACGTTAAAAATGAGTGCACGAAAGCGAAA  >  NC_000913/113015‑113284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`