BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,755,660	T→C	intergenic (‑519/‑38)	fumD ← / → pykF	fumarase D/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,660	0	T	C	100.0%	654.3 / NA	180	intergenic (‑519/‑38)	fumD/pykF	fumarase D/pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (90/90); total (90/90)

Only 100 of 181 total aligned reads displayed.

GCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTG  >  NC_000913/1755528‑1755796

gCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTccc                                                                                                                                   >  1:2316679/1‑140 (MQ=255)

 cACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCa                                                                                                                                  <  2:1186332/140‑1 (MQ=255)

      aCGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCtt                                                                                                                             <  2:1145862/140‑1 (MQ=255)

        gTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTtct                                                                                                                           <  1:1143968/140‑1 (MQ=255)

         ttGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATATCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTtctc                                                                                                                          <  2:3416605/140‑1 (MQ=255)

           gggCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCtt                                                                                                                             <  2:261340/135‑1 (MQ=255)

            ggCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAAc                                                                                                                       >  2:1939602/1‑140 (MQ=255)

               tGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTa                                                                                                                    <  1:1021714/140‑1 (MQ=255)

               tGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTa                                                                                                                    <  1:3211813/140‑1 (MQ=255)

               tGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTa                                                                                                                    <  1:418893/140‑1 (MQ=255)

               tGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTa                                                                                                                    <  1:649200/140‑1 (MQ=255)

                 agaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTaaa                                                                                                                  >  1:2888056/1‑140 (MQ=255)

                 agaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCAATCCTTCTCAACTTaaa                                                                                                                  >  1:840136/1‑140 (MQ=255)

                  gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTa                                                                                                                    >  1:1539952/1‑137 (MQ=255)

                  gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTa                                                                                                                    >  2:2246420/1‑137 (MQ=255)

                  gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTa                                                                                                                    <  2:1539952/137‑1 (MQ=255)

                      caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAaagacta                                                                                                             >  1:1749645/1‑140 (MQ=255)

                       aaGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAaagactaa                                                                                                            <  1:660225/140‑1 (MQ=255)

                         gCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAaagactaaga                                                                                                          <  2:464133/140‑1 (MQ=255)

                         gCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAaagactaaga                                                                                                          <  1:321539/140‑1 (MQ=255)

                         gCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAaagactaaga                                                                                                          <  1:3012712/140‑1 (MQ=255)

                         gCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAaagactaaga                                                                                                          <  2:3341059/140‑1 (MQ=255)

                           acacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTtctc                                                                                                                          <  2:430168/122‑1 (MQ=255)

                           acacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTtctc                                                                                                                          <  2:3369028/122‑1 (MQ=255)

                               aTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                                                                                    <  2:575112/140‑1 (MQ=255)

                               aTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                                                                                    >  1:1834610/1‑140 (MQ=255)

                                 tCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAaagactaaga                                                                                                          >  2:1745260/1‑132 (MQ=255)

                                 tCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATg                                                                                                  >  1:2233037/1‑140 (MQ=255)

                                   ctctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAaagactaaga                                                                                                          >  1:2422848/1‑131 (MQ=255)

                                        cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGa                                                                                           >  1:1442388/1‑140 (MQ=255)

                                         aCGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                                                                                          >  1:2244369/1‑140 (MQ=255)

                                          cGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAcc                                                                                         <  2:213882/140‑1 (MQ=255)

                                           gCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCa                                                                                        >  1:2603243/1‑140 (MQ=255)

                                           gCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCa                                                                                        >  1:1685658/1‑140 (MQ=255)

                                           gCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCa                                                                                        >  2:1046621/1‑140 (MQ=255)

                                           gCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCACTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCa                                                                                        >  1:2269525/1‑140 (MQ=255)

                                                       tCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCa                                                                            >  1:3124977/1‑140 (MQ=255)

                                                                   agagCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGa                                                                >  2:1811550/1‑140 (MQ=255)

                                                                   agagCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGa                                                                >  2:681779/1‑140 (MQ=255)

                                                                     agCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGaaa                                                              >  2:1946433/1‑140 (MQ=255)

                                                                     agCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGaaa                                                              >  2:2968867/1‑140 (MQ=255)

                                                                     agCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGaaa                                                              >  1:1073775/1‑140 (MQ=255)

                                                                       cGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCAATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAAc                                                            <  2:840136/140‑1 (MQ=255)

                                                                        gAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAAcc                                                           >  2:739957/1‑140 (MQ=255)

                                                                         aGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCg                                                          >  2:595023/1‑140 (MQ=255)

                                                                         aGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCg                                                          >  2:1624430/1‑140 (MQ=255)

                                                                         aGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCg                                                          >  1:534306/1‑140 (MQ=255)

                                                                         aGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCg                                                          >  1:1992831/1‑140 (MQ=255)

                                                                         aGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCg                                                          >  2:2874323/1‑140 (MQ=255)

                                                                         aGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCg                                                          >  1:3445993/1‑140 (MQ=255)

                                                                         aGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAACAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCg                                                          >  1:3341525/1‑140 (MQ=255)

                                                                          ggCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATc                                                                       <  1:638973/126‑1 (MQ=255)

                                                                          ggCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATc                                                                       >  2:638973/1‑126 (MQ=255)

                                                                          ggCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGa                                                         >  2:2348713/1‑140 (MQ=255)

                                                                          ggCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGa                                                         <  1:2348713/140‑1 (MQ=255)

                                                                            caccaccacTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAAt                                                       <  2:534306/140‑1 (MQ=255)

                                                                             accaccacTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATc                                                      <  2:118710/140‑1 (MQ=255)

                                                                             accaccacTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATc                                                      <  1:316895/140‑1 (MQ=255)

                                                                                  cacTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAag                                                 <  1:1946433/140‑1 (MQ=255)

                                                                                  cacTTTCGTAATA‑CCGGATTCGCTCTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAag                                                 <  2:3124977/140‑1 (MQ=255)

                                                                                  cacTTTCGTAATA‑CCGGATTCACTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAag                                                 <  2:2269525/140‑1 (MQ=255)

                                                                                        cGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGt                                           <  1:1003335/140‑1 (MQ=255)

                                                                                         gTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGtt                                          <  1:595023/140‑1 (MQ=255)

                                                                                           aaTA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAAt                                                       <  1:1157423/125‑1 (MQ=255)

                                                                                           aaTA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAg                                        <  1:739957/140‑1 (MQ=255)

                                                                                            aTA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGc                                       >  2:2918586/1‑140 (MQ=255)

                                                                                             tA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAAt                                                       >  1:1243774/1‑123 (MQ=255)

                                                                                             tA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAAt                                                       <  2:1243774/123‑1 (MQ=255)

                                                                                              a‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTa                                     <  1:183752/140‑1 (MQ=255)

                                                                                              a‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTa                                     <  2:45366/140‑1 (MQ=255)

                                                                                                   gATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                 >  2:647509/1‑140 (MQ=255)

                                                                                                   gATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                 >  2:17130/1‑140 (MQ=255)

                                                                                                      tCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                              <  1:448824/140‑1 (MQ=255)

                                                                                                        gCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTgg                            <  2:874753/140‑1 (MQ=255)

                                                                                                        gCTTTCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTgg                            >  2:1135496/1‑140 (MQ=255)

                                                                                                           ttCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACg                         <  1:3374949/140‑1 (MQ=255)

                                                                                                           ttCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACg                         <  2:2525164/140‑1 (MQ=255)

                                                                                                           ttCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACg                         >  2:2417345/1‑140 (MQ=255)

                                                                                                           ttCCGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACg                         <  2:170918/140‑1 (MQ=255)

                                                                                                             ccGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCt                       >  1:1366517/1‑140 (MQ=255)

                                                                                                             ccGGCAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCt                       <  1:17130/140‑1 (MQ=255)

                                                                                                                 cAGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                   <  2:2233037/140‑1 (MQ=255)

                                                                                                                  aGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAt                  <  2:368963/140‑1 (MQ=255)

                                                                                                                  aGTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAt                  <  1:1559563/140‑1 (MQ=255)

                                                                                                                   gTGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATg                 <  2:1447579/140‑1 (MQ=255)

                                                                                                                    tGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGa                >  1:3412114/1‑140 (MQ=255)

                                                                                                                    tGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGa                >  1:914957/1‑140 (MQ=255)

                                                                                                                    tGCGCCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGa                >  1:1227155/1‑140 (MQ=255)

                                                                                                                     gcgcCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGaa               <  2:1992831/140‑1 (MQ=255)

                                                                                                                      cgcCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                              >  2:2699076/1‑124 (MQ=255)

                                                                                                                      cgcCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                              <  1:2699076/124‑1 (MQ=255)

                                                                                                                      cgcCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                   <  1:41843/135‑1 (MQ=255)

                                                                                                                      cgcCCAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAc              >  1:2781233/1‑140 (MQ=255)

                                                                                                                        cccAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                     >  1:2937076/1‑131 (MQ=255)

                                                                                                                          cAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa          <  1:2099485/140‑1 (MQ=255)

                                                                                                                          cAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa          <  1:1511684/140‑1 (MQ=255)

                                                                                                                          cAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa          <  2:1842312/140‑1 (MQ=255)

                                                                                                                          cAGAAAGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTa          <  1:902350/140‑1 (MQ=255)

                                                                                                                             aaaGCAAGCTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGc       <  1:1879307/140‑1 (MQ=255)

                                                                                                                              aagcaagcTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCg      >  1:2419243/1‑140 (MQ=255)

GCACTCACGTTGGGCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATA‑CCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTG  >  NC_000913/1755528‑1755796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`