BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,345,512	T→C	R216R (CGT→CGC)	nrdA →	ribonucleoside‑diphosphate reductase 1 subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,345,512	0	T	C	100.0%	454.0 / NA	127	R216R (CGT→CGC)	nrdA	ribonucleoside‑diphosphate reductase 1 subunit alpha
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (60/67); total (60/67)

Only 100 of 139 total aligned reads displayed.

TTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGCGTATTC  >  NC_000913/2345375‑2345648

ttCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCAc                                                                                                                                        >  2:2444225/1‑140 (MQ=255)

   cTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCAcccc                                                                                                                                     >  2:3434423/1‑140 (MQ=255)

   cTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCAcccc                                                                                                                                     <  1:1818011/140‑1 (MQ=255)

     ttATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGa                                                                                                                                   <  1:2904924/140‑1 (MQ=255)

     ttATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGa                                                                                                                                   <  1:119931/140‑1 (MQ=255)

     ttATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGa                                                                                                                                   <  1:209554/140‑1 (MQ=255)

      tataTTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCAcccc                                                                                                                                     <  2:2138728/137‑1 (MQ=255)

          ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGTGCACCCCGACTCGt                                                                                                                              <  1:2334257/140‑1 (MQ=255)

          ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGa                                                                                                                                   <  2:710321/135‑1 (MQ=255)

          ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGa                                                                                                                                   >  1:710321/1‑135 (MQ=255)

          ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGt                                                                                                                              >  2:2743433/1‑140 (MQ=255)

          ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGt                                                                                                                              >  2:2805100/1‑140 (MQ=255)

          ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGt                                                                                                                              >  2:2820738/1‑140 (MQ=255)

          ttCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGt                                                                                                                              >  2:1646330/1‑140 (MQ=255)

           tCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTc                                                                                                                             >  2:2780080/1‑140 (MQ=255)

             tAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAg                                                                                                                           >  2:1886356/1‑140 (MQ=255)

               gTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGa                                                                                                                                   >  2:1153173/1‑130 (MQ=255)

               gTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGa                                                                                                                                   <  1:1153173/130‑1 (MQ=255)

               gTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGtt                                                                                                                         <  2:2547287/140‑1 (MQ=255)

                  gCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAg                                                                                                                      >  2:3124433/1‑140 (MQ=255)

                  gCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAg                                                                                                                      >  2:338096/1‑140 (MQ=255)

                  gCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAg                                                                                                                      <  1:301322/140‑1 (MQ=255)

                  gCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAg                                                                                                                      >  2:930992/1‑140 (MQ=255)

                  gCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAg                                                                                                                      <  1:2120514/140‑1 (MQ=255)

                  gCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAg                                                                                                                      <  1:157978/140‑1 (MQ=255)

                    cgcgTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCt                                                                                                                    <  1:1979766/140‑1 (MQ=255)

                       gTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTcat                                                                                                                 <  2:808787/140‑3 (MQ=255)

                        tGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTg                                                                                                                <  1:1646330/140‑1 (MQ=255)

                        tGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTg                                                                                                                <  1:2444225/140‑1 (MQ=255)

                        tGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTg                                                                                                                <  1:2628215/140‑1 (MQ=255)

                           ttGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGt                                                                                                             <  2:3089865/140‑1 (MQ=255)

                            tGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTa                                                                                                            <  1:2743433/140‑1 (MQ=255)

                                      cTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGt                                                                                                  <  1:3434423/140‑1 (MQ=255)

                                         cccGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCg                                                                                                     <  1:2268659/134‑1 (MQ=255)

                                         cccGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCg                                                                                                     >  2:2268659/1‑134 (MQ=255)

                                         cccGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCg                                                                                               >  2:148270/1‑140 (MQ=255)

                                                 aaaCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGc                                                                                       <  1:338096/140‑1 (MQ=255)

                                                  aaCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGcc                                                                                      <  1:57950/140‑1 (MQ=255)

                                                   aCGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCt                                                                                     >  2:2983313/1‑140 (MQ=255)

                                                    cgcgCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTg                                                                                    >  2:2453368/1‑140 (MQ=255)

                                                       gcCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGAt                                                                                 <  1:2233227/140‑1 (MQ=255)

                                                        ccTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTg                                                                                                 <  1:2805277/123‑1 (MQ=255)

                                                        ccTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGAtt                                                                                >  2:2582727/1‑140 (MQ=255)

                                                        ccTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGAtt                                                                                <  2:461685/140‑1 (MQ=255)

                                                            cAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCAt                                                                            >  2:2925404/1‑140 (MQ=255)

                                                              atatGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCa                                                                          <  1:2495475/140‑1 (MQ=255)

                                                              atatGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCa                                                                          <  1:1045546/140‑1 (MQ=255)

                                                              atatGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCa                                                                          <  1:3406694/140‑1 (MQ=255)

                                                               tatGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCaa                                                                         >  2:417026/1‑140 (MQ=255)

                                                                  gtgAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGc                                                                      <  1:2805100/140‑1 (MQ=255)

                                                                   tgAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGcc                                                                     <  1:417026/140‑1 (MQ=255)

                                                                   tgAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGcc                                                                     >  2:250145/1‑140 (MQ=255)

                                                                     aaGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCAc                                                                   >  2:2808677/1‑140 (MQ=255)

                                                                     aaGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCAc                                                                   >  2:2146317/1‑140 (MQ=255)

                                                                     aaGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCAc                                                                   >  2:2742077/1‑140 (MQ=255)

                                                                     aaGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCAc                                                                   >  2:2313735/1‑140 (MQ=255)

                                                                      aGCGTTTTTACGTCGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGGGACAGCCTGGATTCCATCAACGCCAcc                                                                  >  2:280430/1‑140 (MQ=255)

                                                                      aGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCAcc                                                                  >  1:2075486/1‑140 (MQ=255)

                                                                      aGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCAcc                                                                  >  2:2030077/1‑140 (MQ=255)

                                                                       gCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCt                                                                 >  2:3353945/1‑140 (MQ=255)

                                                                        cGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTc                                                                <  1:2758375/140‑1 (MQ=255)

                                                                         gTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTcc                                                               >  2:1639996/1‑140 (MQ=255)

                                                                           ttttACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAg                                                             <  1:1088205/140‑1 (MQ=255)

                                                                           ttttACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAg                                                             >  2:2241185/1‑140 (MQ=255)

                                                                            tttACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCa                                                                          >  2:181342/1‑126 (MQ=255)

                                                                            tttACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCa                                                                          <  1:181342/126‑1 (MQ=255)

                                                                             ttACGACGCGGTTTCCACATTTAAAATTTCTCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAgcg                                                           <  1:2154309/140‑1 (MQ=255)

                                                                             ttACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAgcg                                                           <  1:854194/140‑1 (MQ=255)

                                                                              tACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAgcgc                                                          <  1:2925404/140‑1 (MQ=255)

                                                                                cgacgCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGa                                                        <  1:2582727/140‑1 (MQ=255)

                                                                                 gacgCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGAt                                                       <  1:2438620/140‑1 (MQ=255)

                                                                                   cgcgGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTg                                                     >  2:2116734/1‑140 (MQ=255)

                                                                                     cgGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGa                                                        >  2:3483332/1‑135 (MQ=255)

                                                                                      ggTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTa                                                  <  1:2246076/140‑1 (MQ=255)

                                                                                         ttCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAAt                                               <  2:2075486/140‑1 (MQ=255)

                                                                                         ttCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAAt                                               <  1:1738148/140‑1 (MQ=255)

                                                                                             acaTTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGt                                           >  2:1825544/1‑140 (MQ=255)

                                                                                                tttAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGa                                                        >  2:719603/1‑124 (MQ=255)

                                                                                                tttAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGa                                                        <  1:719603/124‑1 (MQ=255)

                                                                                                tttAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTc                                        >  2:1253418/1‑140 (MQ=255)

                                                                                                  tAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTccc                                      <  1:792565/140‑1 (MQ=255)

                                                                                                     aaTTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGc                                   <  1:3353945/140‑1 (MQ=255)

                                                                                                                cGACGCCAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATc                        <  1:19391/140‑1 (MQ=255)

                                                                                                                     ccAATCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCAt                   <  2:58373/140‑1 (MQ=255)

                                                                                                                         tCATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAAc               >  1:426333/1‑140 (MQ=255)

                                                                                                                          cATGTCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACg              <  1:3312874/140‑1 (MQ=255)

                                                                                                                              tCCGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCgg          >  2:301977/1‑140 (MQ=255)

                                                                                                                               ccGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCgg          >  1:3473781/1‑139 (MQ=255)

                                                                                                                               ccGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCgg          <  2:3473781/139‑1 (MQ=255)

                                                                                                                                cGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAGTACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGc        >  1:1426522/1‑140 (MQ=255)

                                                                                                                                cGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGc        >  2:1402078/1‑140 (MQ=255)

                                                                                                                                cGGCGTGCGCACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGc        >  1:106947/1‑140 (MQ=255)

                                                                                                                                   cgtgcgcACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGCGTa     >  2:1161404/1‑140 (MQ=255)

                                                                                                                                   cgtgcgcACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGCGTa     >  2:1284130/1‑140 (MQ=255)

                                                                                                                                   cgtgcgcACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGCGTa     >  1:293696/1‑140 (MQ=255)

                                                                                                                                   cgtgcgcACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGCGTa     >  1:2591748/1‑140 (MQ=255)

                                                                                                                                    gtgcgcACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGCGTAt    >  2:3104825/1‑140 (MQ=255)

                                                                                                                                      gcgcACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGCGTATTc  <  1:301977/140‑1 (MQ=255)

                                                                                                                                      gcgcACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGCGTATTc  <  2:1426522/140‑1 (MQ=255)

                                                                                                                                      gcgcACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGCGTATTc  <  2:106947/140‑1 (MQ=255)

TTCCTTTATATTCTAGTTGCCGCGTGCTTGTTCTCGAACTACCCGCGTGAAACGCGCCTGCAATATGTGAAGCGTTTTTACGACGCGGTTTCCACATTTAAAATTTCGCTGCCGACGCCAATCATGTCCGGCGTGCGTACCCCGACTCGTCAGTTCAGCTCCTGCGTACTGATCGAGTGCGGTGACAGCCTGGATTCCATCAACGCCACCTCCAGCGCGATTGTTAAATACGTTTCCCAGCGTGCCGGGATCGGCATCAACGCCGGGCGTATTC  >  NC_000913/2345375‑2345648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`