BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,400,358	G→A	H400Y (CAC→TAC)	nuoF ←	NADH:quinone oxidoreductase subunit F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,400,358	0	G	A	100.0%	621.2 / NA	172	H400Y (CAC→TAC)	nuoF	NADH:quinone oxidoreductase subunit F
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (82/90); total (82/90)

Only 100 of 174 total aligned reads displayed.

CCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGT  >  NC_000913/2400221‑2400493

ccAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTagg                                                                                                                                       >  1:1501289/1‑140 (MQ=255)

     gcTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAg                                                                                                                                  <  2:1605487/140‑1 (MQ=255)

     gcTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAg                                                                                                                                  <  2:2738535/140‑1 (MQ=255)

      ctctTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGa                                                                                                                                 >  2:2190841/1‑140 (MQ=255)

              gcagGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTa                                                                                                                         >  2:1917908/1‑140 (MQ=255)

              gcagGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTa                                                                                                                         >  2:1844507/1‑140 (MQ=255)

              gcagGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTa                                                                                                                         >  2:1716156/1‑140 (MQ=255)

                gggTTCGGCGGAACCCAATAAATAAAATGGTTATTGCTGACGGGCTGTTTGATCCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTAcc                                                                                                                       <  2:3015300/139‑1 (MQ=255)

                agGTTCGGCTGAATCCCATTAATCAAATGGGTTTTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTAcc                                                                                                                       <  2:1523314/140‑1 (MQ=255)

                agGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTAcc                                                                                                                       <  2:2832963/140‑1 (MQ=255)

                agGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTAcc                                                                                                                       <  1:2927950/140‑1 (MQ=255)

                agGTTCGGCTGAATCCCAATAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTAcc                                                                                                                       <  2:70728/140‑1 (MQ=255)

                  gTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCg                                                                                                                     <  1:520844/140‑1 (MQ=255)

                       gCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCt                                                                                                                >  1:1393150/1‑140 (MQ=255)

                       gCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCt                                                                                                                <  2:2022951/140‑1 (MQ=255)

                       gCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCt                                                                                                                <  1:1653446/140‑1 (MQ=255)

                           aaTCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGa                                                                                                            <  2:2811487/140‑1 (MQ=255)

                            aTCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGaa                                                                                                           >  2:2432630/1‑140 (MQ=255)

                                cATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGa                                                                                                       <  2:228770/140‑1 (MQ=255)

                                 aTTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGac                                                                                                      <  1:1422344/140‑1 (MQ=255)

                                   tAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGacac                                                                                                    <  2:402199/140‑1 (MQ=255)

                                   tAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGacac                                                                                                    <  1:2279042/140‑1 (MQ=255)

                                   tAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCACCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGacac                                                                                                    <  1:3294667/140‑1 (MQ=255)

                                   tAATCAAATGGGTATTGCTGAACGGCTGTTTGATCCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGacac                                                                                                    <  1:3434273/140‑1 (MQ=255)

                                     aTCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAg                                                                                                  <  2:2250681/140‑1 (MQ=255)

                                     aTCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAg                                                                                                  <  1:1360831/140‑1 (MQ=255)

                                       cAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGtt                                                                                                >  2:1233164/1‑140 (MQ=255)

                                          aTGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCt                                                                                             >  2:2104574/1‑140 (MQ=255)

                                           tGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTc                                                                                            >  1:241636/1‑140 (MQ=255)

                                            gggTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCa                                                                                           <  1:1686738/140‑1 (MQ=255)

                                             ggTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCaa                                                                                          <  2:2144375/140‑1 (MQ=255)

                                             ggTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACCGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCaa                                                                                          <  2:3217923/140‑1 (MQ=255)

                                                aTTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAgtg                                                                                       >  1:1152057/1‑140 (MQ=255)

                                                aTTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAgtg                                                                                       >  1:2229463/1‑140 (MQ=255)

                                                  tGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGtt                                                                                     >  1:422271/1‑140 (MQ=255)

                                                    cTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTc                                                                                            <  1:2733118/131‑1 (MQ=255)

                                                    cTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTc                                                                                   >  1:791922/1‑140 (MQ=255)

                                                    cTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTc                                                                                   >  1:2395381/1‑140 (MQ=255)

                                                      gAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGa                                                                                 <  1:2667122/140‑1 (MQ=255)

                                                       aaCGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACTAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGat                                                                                >  1:124567/1‑140 (MQ=255)

                                                       aaCGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGat                                                                                >  1:460418/1‑140 (MQ=255)

                                                         cGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGatat                                                                              >  1:2137192/1‑140 (MQ=255)

                                                         cGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGatat                                                                              >  1:1278375/1‑140 (MQ=255)

                                                         cGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGatat                                                                              >  1:1724451/1‑140 (MQ=255)

                                                           gCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCg                                                                            >  1:1239039/1‑140 (MQ=255)

                                                            cTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGc                                                                           >  2:3333382/1‑140 (MQ=255)

                                                            cTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGc                                                                           >  1:2451373/1‑140 (MQ=255)

                                                            cTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGc                                                                           >  1:3193449/1‑140 (MQ=255)

                                                            cTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGc                                                                           <  2:2497974/140‑1 (MQ=255)

                                                               tttGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCg                                                                        >  1:2523564/1‑140 (MQ=255)

                                                                ttGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCgg                                                                       <  2:709199/140‑1 (MQ=255)

                                                                 tGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGc                                                                      <  2:241636/140‑1 (MQ=255)

                                                                  gATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCt                                                                     <  1:2104574/140‑1 (MQ=255)

                                                                  gATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCt                                                                     <  2:2523564/140‑1 (MQ=255)

                                                                  gATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCt                                                                     <  2:2611041/140‑1 (MQ=255)

                                                                   aTTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTg                                                                    <  2:2395381/140‑1 (MQ=255)

                                                                   aTTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTg                                                                    <  2:791922/140‑1 (MQ=255)

                                                                      cccGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGAcc                                                                 >  1:3235972/1‑140 (MQ=255)

                                                                        cGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCtt                                                               <  1:1917908/140‑1 (MQ=255)

                                                                        cGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCtt                                                               <  2:460418/140‑1 (MQ=255)

                                                                         gCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTc                                                              >  2:3202404/1‑140 (MQ=255)

                                                                          ccTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTca                                                             >  1:2253565/1‑140 (MQ=255)

                                                                             cAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTcacca                                                          <  2:2238103/140‑1 (MQ=255)

                                                                             cAAATTCTGCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTACACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTcacca                                                          >  2:2011950/1‑140 (MQ=255)

                                                                                    ttcGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCa                                                   >  2:1651517/1‑140 (MQ=255)

                                                                                     tcGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAg                                                  >  1:929276/1‑140 (MQ=255)

                                                                                        cgGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCGCCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAgcgc                                               >  2:1678870/1‑140 (MQ=255)

                                                                                                    aTGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAAtttt                                   >  2:2228822/1‑140 (MQ=255)

                                                                                                     tGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTc                                  >  2:548717/1‑140 (MQ=255)

                                                                                                     tGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTc                                  >  1:2806512/1‑140 (MQ=255)

                                                                                                      ggCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCa                                 <  2:437036/140‑1 (MQ=255)

                                                                                                      ggCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCa                                 <  1:1156292/140‑1 (MQ=255)

                                                                                                      ggCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCa                                 <  1:1639967/140‑1 (MQ=255)

                                                                                                        cgcTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACg                               >  2:1287442/1‑140 (MQ=255)

                                                                                                        cgcTCTGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACg                               >  1:774844/1‑140 (MQ=255)

                                                                                                          ctctGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCt                             >  1:2417832/1‑140 (MQ=255)

                                                                                                          ctctGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCt                             >  2:3286974/1‑140 (MQ=255)

                                                                                                           tctGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTc                            <  1:1844507/140‑1 (MQ=255)

                                                                                                           tctGTAACGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTc                            <  1:2646520/140‑1 (MQ=255)

                                                                                                                  cGGCTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCa                     <  2:1198645/140‑1 (MQ=255)

                                                                                                                     cTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGAc                  >  2:2520066/1‑140 (MQ=255)

                                                                                                                     cTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGAc                  <  2:3181773/140‑1 (MQ=255)

                                                                                                                     cTCCACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGAc                  >  2:87261/1‑140 (MQ=255)

                                                                                                                       ccACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCa                                 <  2:1381894/123‑1 (MQ=255)

                                                                                                                       ccACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCg                >  1:715250/1‑140 (MQ=255)

                                                                                                                       ccACTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCg                >  2:1737939/1‑140 (MQ=255)

                                                                                                                       ccACTGCACCAGGTGCGCAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCg                >  1:647318/1‑140 (MQ=255)

                                                                                                                         aCTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTc              >  1:2938692/1‑140 (MQ=255)

                                                                                                                         aCTGCACCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTc              >  1:1224294/1‑140 (MQ=255)

                                                                                                                              aCCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGc         <  2:1152057/140‑1 (MQ=255)

                                                                                                                              aCCAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGc         <  1:87261/140‑1 (MQ=255)

                                                                                                                               ccAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCa        <  1:166219/140‑1 (MQ=255)

                                                                                                                               ccAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCa        <  1:3144603/140‑1 (MQ=255)

                                                                                                                               ccAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCa        <  1:202320/140‑1 (MQ=255)

                                                                                                                               ccAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCa        <  1:2190841/140‑1 (MQ=255)

                                                                                                                                cAGGTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGc         <  2:2169761/138‑1 (MQ=255)

                                                                                                                                  ggTGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACgg     >  2:147736/1‑140 (MQ=255)

                                                                                                                                    tGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCa        >  1:2016577/1‑135 (MQ=255)

                                                                                                                                    tGCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCa        <  2:2016577/135‑1 (MQ=255)

                                                                                                                                     gCGTAGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCa        >  1:44885/1‑134 (MQ=255)

CCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGT  >  NC_000913/2400221‑2400493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`