Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,696,672 T→C L291S (TTA→TCA)  mltF → membrane‑bound lytic murein transglycosylase F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,696,6720TC100.0% 751.7 / NA 205L291S (TTA→TCA) mltFmembrane‑bound lytic murein transglycosylase F
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (104/101);  total (104/101)

Only 100 of 206 total aligned reads displayed.

CGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCATGATGATGTTAACCAAAA  >  NC_000913/2696538‑2696806
                                                                                                                                      |                                                                                                                                      
cGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGc                                                                                                                                   <  2:3233456/140‑1 (MQ=255)
 gACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGcc                                                                                                                                  <  2:1137492/140‑1 (MQ=255)
 gACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGcc                                                                                                                                  <  2:1244298/140‑1 (MQ=255)
      cttcAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGt                                                                                                                             <  1:179232/140‑1 (MQ=255)
       ttcAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGtt                                                                                                                            >  2:1621083/1‑140 (MQ=255)
         cAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTg                                                                                                                          >  1:2240447/1‑140 (MQ=255)
         cAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTg                                                                                                                          >  1:545940/1‑140 (MQ=255)
            cGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGcc                                                                                                                                  >  1:1414189/1‑129 (MQ=255)
               aatgaatgAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAAt                                                                                                                    <  2:626800/140‑1 (MQ=255)
                atgaatgaAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATa                                                                                                                   >  1:3002599/1‑140 (MQ=255)
                atgaatgaAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATa                                                                                                                   >  1:2291083/1‑140 (MQ=255)
                         gACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATAc                                                                                                                  >  1:1596993/1‑132 (MQ=255)
                         gACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATAc                                                                                                                  <  2:1596993/132‑1 (MQ=255)
                           cGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCga                                                                                                             <  2:305441/135‑1 (MQ=255)
                           cGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCga                                                                                                             >  1:305441/1‑135 (MQ=255)
                           cGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGaaa                                                                                                        >  1:1678160/1‑140 (MQ=255)
                             gTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAAtt                                                                                                      >  2:1876153/1‑140 (MQ=255)
                             gTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAAtt                                                                                                      >  2:1291717/1‑140 (MQ=255)
                             gTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAAtt                                                                                                      >  1:1078616/1‑140 (MQ=255)
                              tACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCg                                                                                                              >  2:1671187/1‑131 (MQ=255)
                              tACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTg                                                                                                     >  2:3283903/1‑140 (MQ=255)
                                 gCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACt                                                                                                  <  2:109213/140‑1 (MQ=255)
                                        cGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGttt                                                                                           >  2:1655516/1‑140 (MQ=255)
                                           aTTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCt                                                                                        >  2:1790971/1‑140 (MQ=255)
                                           aTTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCt                                                                                        >  2:2218284/1‑140 (MQ=255)
                                              gAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGc                                                                                     <  1:2489402/140‑1 (MQ=255)
                                                agagAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCg                                                                                   <  1:1817786/140‑1 (MQ=255)
                                                     aaTACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTgg                                                                                                >  1:913067/1‑122 (MQ=255)
                                                     aaTACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTAtt                                                                              >  1:2035765/1‑140 (MQ=255)
                                                     aaTACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTAtt                                                                              >  2:1290432/1‑140 (MQ=255)
                                                          cTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACA‑‑TTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATc                                                                       <  2:192223/140‑1 (MQ=255)
                                                           tGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTAt                                                                        >  2:767635/1‑140 (MQ=255)
                                                            ggggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATc                                                                       <  2:1699565/140‑1 (MQ=255)
                                                            ggggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATc                                                                       >  1:1573247/1‑140 (MQ=255)
                                                            ggggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATc                                                                       >  1:1699565/1‑140 (MQ=255)
                                                            ggggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATc                                                                       >  1:1337885/1‑140 (MQ=255)
                                                            ggggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATc                                                                       >  2:411322/1‑140 (MQ=255)
                                                             gggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCa                                                                      >  1:2369714/1‑140 (MQ=255)
                                                              ggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAg                                                                     >  2:1015692/1‑140 (MQ=255)
                                                              ggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAg                                                                     <  2:545940/140‑1 (MQ=255)
                                                              ggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAg                                                                     >  2:808623/1‑140 (MQ=255)
                                                              ggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAg                                                                     >  1:3319695/1‑140 (MQ=255)
                                                              ggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAg                                                                     >  2:2857682/1‑140 (MQ=255)
                                                              ggCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAg                                                                     >  2:586773/1‑140 (MQ=255)
                                                               gCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAgg                                                                    <  1:2163014/140‑1 (MQ=255)
                                                                 aTGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGaa                                                                  <  2:1545913/140‑1 (MQ=255)
                                                                  tGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAAt                                                                 <  1:1291717/140‑1 (MQ=255)
                                                                  tGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAAt                                                                 >  2:895720/1‑140 (MQ=255)
                                                                  tGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAAt                                                                 >  1:2433397/1‑140 (MQ=255)
                                                                  tGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAAt                                                                 >  1:3423255/1‑140 (MQ=255)
                                                                   ggCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATc                                                                >  1:3457799/1‑140 (MQ=255)
                                                                   ggCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATc                                                                >  1:1173891/1‑140 (MQ=255)
                                                                   ggCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTCGCTGGCCGCTATTGCTTATCAGGAATc                                                                >  2:976619/1‑140 (MQ=255)
                                                                    gCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTTGGTGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCg                                                               >  1:16390/1‑140 (MQ=255)
                                                                      gatgatTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCa                                                             <  1:2857682/140‑1 (MQ=255)
                                                                      gatgatTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCa                                                             <  2:33254/140‑1 (MQ=255)
                                                                      gatgatTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTCGCTGGCCGCTATTGCTTATCAGGAATCGCa                                                             <  1:976619/140‑1 (MQ=255)
                                                                       atgatTTTGATTACGTCGATACGCGCACATTTTTACGCGTCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCAc                                                            >  2:1405586/1‑140 (MQ=255)
                                                                       atgatTTTGACTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCAc                                                            >  1:1663056/1‑140 (MQ=255)
                                                                        tgcttttGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGATACTGCCGCAGTCAAAGCCCCTGTTTGAGCAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACt                                                           >  2:1440512/4‑140 (MQ=255)
                                                                          atTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTgg                                                         <  1:867266/140‑1 (MQ=255)
                                                                                aTTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGca                                                   <  2:2240447/140‑1 (MQ=255)
                                                                                aTTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGca                                                   <  2:1173891/140‑1 (MQ=255)
                                                                                aTTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGca                                                   <  1:3299412/140‑1 (MQ=255)
                                                                                aTTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGca                                                   >  1:5604/1‑140 (MQ=255)
                                                                                  tACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGcaca                                                 <  2:3002599/140‑1 (MQ=255)
                                                                                    cGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAgg                                               >  2:1499267/1‑140 (MQ=255)
                                                                                     gTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGc                                              >  1:2580394/1‑140 (MQ=255)
                                                                                     gTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGc                                              <  2:122027/140‑1 (MQ=255)
                                                                                     gTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGc                                              <  2:2035765/140‑1 (MQ=255)
                                                                                     gTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGACGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGc                                              >  2:2448134/1‑140 (MQ=255)
                                                                                      tCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGcc                                             >  2:2580530/1‑140 (MQ=255)
                                                                                      tCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGcc                                             <  1:1621083/140‑1 (MQ=255)
                                                                                         aTACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACt                                          <  1:1015692/140‑1 (MQ=255)
                                                                                         aTACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACt                                          <  1:1499267/140‑1 (MQ=255)
                                                                                         aTACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACt                                          <  2:3319695/140‑1 (MQ=255)
                                                                                           aCGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTc                                        <  1:441558/140‑1 (MQ=255)
                                                                                              cgcACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCAcc                                     >  1:2725909/1‑140 (MQ=255)
                                                                                                          cgcgCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTgcg                         <  1:1876153/140‑1 (MQ=255)
                                                                                                          cgcgCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTgcg                         <  2:1337885/140‑1 (MQ=255)
                                                                                                          cgcgCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTgcg                         <  2:3457799/140‑1 (MQ=255)
                                                                                                          cgcgCCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTgcg                         <  1:2980042/140‑1 (MQ=255)
                                                                                                            cgcCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGcgcg                       <  2:2697364/140‑1 (MQ=255)
                                                                                                            cgcCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGcgcg                       <  2:84903/140‑1 (MQ=255)
                                                                                                             gcCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCgg                      >  1:3175517/1‑140 (MQ=255)
                                                                                                             gcCGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCgg                      <  1:808623/140‑1 (MQ=255)
                                                                                                               cGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCa                    >  2:972477/1‑140 (MQ=255)
                                                                                                               cGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCa                    >  1:1050284/1‑140 (MQ=255)
                                                                                                               cGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCa                    >  2:2734224/1‑140 (MQ=255)
                                                                                                               cGTCGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCa                    >  2:1718868/1‑140 (MQ=255)
                                                                                                                  cGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCatga                 <  2:3423255/140‑1 (MQ=255)
                                                                                                                  cGATGCGGTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCatga                 <  2:2369714/140‑1 (MQ=255)
                                                                                                                        ggTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCATGATGATGt           <  2:3015502/140‑1 (MQ=255)
                                                                                                                        ggTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCATGATGATGt           <  1:1241102/140‑1 (MQ=255)
                                                                                                                         gTACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCATGATGATGtt          >  2:1353041/1‑140 (MQ=255)
                                                                                                                          tACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCATGATGATGTTa         <  1:1718868/140‑1 (MQ=255)
                                                                                                                          tACTGCCGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCATGATGATGTTa         <  2:1830552/140‑1 (MQ=255)
                                                                                                                               ccGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCATGATGATGTTAACCaa    >  1:2636164/1‑140 (MQ=255)
                                                                                                                               ccGCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCATGATGATGTTAACCaa    >  2:2946691/1‑140 (MQ=255)
                                                                                                                                 gCAGTCAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCATGATGATGTTAACCaaaa  >  1:3187553/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CGACTTCTTCAACGAAATGAATGAAGACGGTACGCTGGCACGCATTGAAGAGAAATACCTGGGGCATGGCGATGATTTTGATTACGTCGATACGCGCACATTTTTACGCGCCGTCGATGCGGTACTGCCGCAGTTAAAGCCCCTGTTTGAGAAATACGCCGAAGAAATTGACTGGCGTTTGCTGGCCGCTATTGCTTATCAGGAATCGCACTGGGATGCACAGGCCACTTCACCGACGGGTGTGCGCGGCATGATGATGTTAACCAAAA  >  NC_000913/2696538‑2696806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑