Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,697,293 A→G N498S (AAC→AGC)  mltF → membrane‑bound lytic murein transglycosylase F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,697,2930AG100.0% 530.4 / NA 145N498S (AAC→AGC) mltFmembrane‑bound lytic murein transglycosylase F
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (73/72);  total (73/72)

Only 100 of 146 total aligned reads displayed.

GGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCA  >  NC_000913/2697156‑2697427
                                                                                                                                         |                                                                                                                                      
gggTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAATTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCt                                                                                                                                      >  1:1715083/1‑140 (MQ=255)
  gTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTAt                                                                                                                                    <  2:3412055/140‑1 (MQ=255)
  gTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTAt                                                                                                                                    <  2:271667/140‑1 (MQ=255)
    tATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTAttt                                                                                                                                  >  1:294514/1‑140 (MQ=255)
    tATCTGCAAGAG‑AAGAGAAGCAGGCTACAGAAGCGGCGATACAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTg                                                                                                                                 <  1:1252782/140‑1 (MQ=255)
       cTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGAc                                                                                                                               <  1:2498329/140‑1 (MQ=255)
        tGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAATTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGAcc                                                                                                                              <  2:1715083/140‑1 (MQ=255)
        tGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCTTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGAcc                                                                                                                              >  2:495650/1‑140 (MQ=255)
        tGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGAcc                                                                                                                              <  1:2391051/140‑1 (MQ=255)
          cAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCa                                                                                                                            <  1:2150527/140‑1 (MQ=255)
           aagagaaagagaAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCAt                                                                                                                           >  1:342332/1‑140 (MQ=255)
           aagagaaagagaAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCAt                                                                                                                           >  2:1617600/1‑140 (MQ=255)
           aagagaaagagaAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCAt                                                                                                                           >  1:2567519/1‑140 (MQ=255)
             gagaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATtc                                                                                                                         >  1:816208/1‑140 (MQ=255)
             gagaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATtc                                                                                                                         >  2:2264403/1‑140 (MQ=255)
             gagaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATtc                                                                                                                         <  1:2264403/140‑1 (MQ=255)
             gagaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATtc                                                                                                                         <  2:2499654/140‑1 (MQ=255)
             gagaaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATtc                                                                                                                         <  1:979792/140‑1 (MQ=255)
                aaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGa                                                                                                                                <  2:3081369/130‑1 (MQ=255)
                aaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGa                                                                                                                                >  1:3081369/1‑130 (MQ=255)
                aaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTcc                                                                                                                      <  2:1293368/140‑1 (MQ=255)
                aaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTcc                                                                                                                      >  1:1179809/1‑140 (MQ=255)
                aaagagaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTcc                                                                                                                      <  1:142868/140‑1 (MQ=255)
                    agaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCctct                                                                                                                  <  2:700442/140‑1 (MQ=255)
                    agaaGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCctct                                                                                                                  >  2:1722271/1‑140 (MQ=255)
                       aGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGAcc                                                                                                                              <  2:2308707/125‑1 (MQ=255)
                       aGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGAcc                                                                                                                              >  1:2308707/1‑125 (MQ=255)
                        gCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTctgc                                                                                                              >  1:2641395/1‑140 (MQ=255)
                        gCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTctgc                                                                                                              >  1:2957325/1‑140 (MQ=255)
                         cAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTctgct                                                                                                             <  1:2798225/140‑1 (MQ=255)
                         cAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTctgct                                                                                                             <  2:1179809/140‑1 (MQ=255)
                         cAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTctgct                                                                                                             >  1:3307161/1‑140 (MQ=255)
                         cAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTctgct                                                                                                             >  2:1229325/1‑140 (MQ=255)
                          aGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTctgctg                                                                                                            <  2:2017775/140‑1 (MQ=255)
                          aGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTctgctg                                                                                                            >  1:2017775/1‑140 (MQ=255)
                          aGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTctgctg                                                                                                            <  2:1458847/140‑1 (MQ=255)
                            gCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTcc                                                                                                                      <  1:2928589/128‑1 (MQ=255)
                            gCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGtt                                                                                                          <  1:1852442/140‑1 (MQ=255)
                            gCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGtt                                                                                                          <  1:1285332/140‑1 (MQ=255)
                             cTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGttt                                                                                                         >  1:662904/1‑140 (MQ=255)
                                 aGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCa                                                                                                     <  2:2567519/140‑1 (MQ=255)
                                     gcggcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGaa                                                                                                 <  2:3181149/140‑1 (MQ=255)
                                     gcggcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGaa                                                                                                 <  2:75169/140‑1 (MQ=255)
                                     gcggcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGaa                                                                                                 <  2:949788/140‑1 (MQ=255)
                                      cggcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTctgct                                                                                                             <  2:2954267/127‑1 (MQ=255)
                                      cggcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTctgct                                                                                                             >  1:2954267/1‑127 (MQ=255)
                                      cggcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGaaa                                                                                                >  1:566611/1‑140 (MQ=255)
                                      cggcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGaaa                                                                                                >  2:2862272/1‑140 (MQ=255)
                                      cggcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGaaa                                                                                                >  2:533219/1‑140 (MQ=255)
                                        gcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGaa                                                                                                 >  1:2449862/1‑137 (MQ=255)
                                        gcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAgg                                                                                              >  2:2090714/1‑140 (MQ=255)
                                        gcgATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAgg                                                                                              >  1:357755/1‑140 (MQ=255)
                                          gATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGaa                                                                                                 >  2:2887475/1‑135 (MQ=255)
                                                  tGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAagag                                                                                    >  1:867321/1‑140 (MQ=255)
                                                   ggCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAgaga                                                                                   >  2:1903293/1‑140 (MQ=255)
                                                   ggCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAgaga                                                                                   >  2:2424190/1‑140 (MQ=255)
                                                     cgcAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGaaa                                                                                 <  1:3024315/140‑1 (MQ=255)
                                                        aGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAag                                                                                      <  1:167807/132‑1 (MQ=255)
                                                          gATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACaaaa                                                                            >  2:2383519/1‑140 (MQ=255)
                                                               tCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCTTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAAt                                                                       <  2:1152825/140‑1 (MQ=255)
                                                               tCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAAt                                                                       <  1:3309656/140‑1 (MQ=255)
                                                               tCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAAt                                                                       <  2:357755/140‑1 (MQ=255)
                                                               tCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAAt                                                                       <  2:948613/140‑1 (MQ=255)
                                                               tCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAAt                                                                       <  1:569048/140‑1 (MQ=255)
                                                                    cggTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTc                                                                  >  1:1737352/1‑140 (MQ=255)
                                                                    cggTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTc                                                                  <  1:1983076/140‑1 (MQ=255)
                                                                    cggTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTc                                                                  >  2:2011612/1‑140 (MQ=255)
                                                                              tACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTgcg                                                        >  1:320436/1‑140 (MQ=255)
                                                                              tACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTgcg                                                        >  2:1886365/1‑140 (MQ=255)
                                                                                cGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCt                                                      >  1:360755/1‑140 (MQ=255)
                                                                                cGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCt                                                      <  1:1229325/140‑1 (MQ=255)
                                                                                     ttGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCtttttt                                                 <  2:816208/140‑1 (MQ=255)
                                                                                       gggCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCt                                               <  2:294514/140‑1 (MQ=255)
                                                                                        ggCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTg                                              >  2:2606484/1‑140 (MQ=255)
                                                                                        ggCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTg                                              <  2:2866683/140‑1 (MQ=255)
                                                                                          cAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTgcg                                            <  2:360755/140‑1 (MQ=255)
                                                                                             agagAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCtt                                         >  1:1762750/1‑140 (MQ=255)
                                                                                              gagaAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCttt                                        <  1:2090714/140‑1 (MQ=255)
                                                                                              gagaAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCttt                                        <  1:65472/140‑1 (MQ=255)
                                                                                               agaAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTa                                       >  2:604547/1‑140 (MQ=255)
                                                                                                         ttttCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTggc                             >  1:1195930/1‑140 (MQ=255)
                                                                                                         ttttCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTggc                             >  2:3394001/1‑140 (MQ=255)
                                                                                                           ttCTCTCGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTggcgg                           >  1:1392454/1‑140 (MQ=255)
                                                                                                             ctctcGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg                         <  1:3394001/140‑1 (MQ=255)
                                                                                                              tctcGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGgcgc                        >  2:2036893/1‑140 (MQ=255)
                                                                                                               ctcGTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCa                       <  2:3471555/140‑1 (MQ=255)
                                                                                                                  gTTTCTTTCCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc                    >  2:1772773/1‑140 (MQ=255)
                                                                                                                   tttctttcCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCg                   <  1:1617600/140‑1 (MQ=255)
                                                                                                                     tctttcCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGaa                 >  2:601348/1‑140 (MQ=255)
                                                                                                                     tctttcCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGaa                 >  2:1907017/1‑140 (MQ=255)
                                                                                                                       tttcCCAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAaag               >  2:2560433/1‑140 (MQ=255)
                                                                                                                          cccAGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAaagaag            >  2:1840661/1‑140 (MQ=255)
                                                                                                                             aGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCa         <  1:1772773/140‑1 (MQ=255)
                                                                                                                             aGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCa         <  2:2540723/140‑1 (MQ=255)
                                                                                                                             aGTCGTCATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCa         <  2:817174/140‑1 (MQ=255)
                                                                                                                                 gtcATCAAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGa     >  1:1604423/1‑140 (MQ=255)
                                                                                                                                  tcatcaAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAg    >  1:2757289/1‑140 (MQ=255)
                                                                                                                                   catcaAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGc   >  2:1869639/1‑140 (MQ=255)
                                                                                                                                    atcaAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCa  >  2:1932020/1‑140 (MQ=255)
                                                                                                                                    atcaAGCTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCa  >  1:2616190/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GGGTTATCTGCAAGAGAAAGAGAAGCAGGCTACAGAAGCGGCGATGCAACTGGCGCAGGATTATCCGGCGGTATCGCCTACGGAGTTGGGCAAAGAGAAATTTCCTTTTCTCTCGTTTCTTTCCCAGTCGTCATCAAACTATTTGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGTCACTGAGCA  >  NC_000913/2697156‑2697427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑