BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,845,936	T→C	N179S (AAC→AGC)	hycE ←	formate hydrogenlyase subunit HycE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,845,936	0	T	C	100.0%	501.6 / NA	140	N179S (AAC→AGC)	hycE	formate hydrogenlyase subunit HycE
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (64/76); total (64/76)

Only 100 of 144 total aligned reads displayed.

CCGCCAGTTTTTCCATGCCGCGATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGTTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCATCCGGCAGCACCAGA  >  NC_000913/2845799‑2846070

ccGCCAGTTTTTCCATGCCGCGATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGctt                                                                                                                                      >  2:2638903/1‑140 (MQ=255)

 cGCCAGTTTTTCCATGCCGCGATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGcttt                                                                                                                                     >  1:2139767/1‑140 (MQ=255)

      gTTTTTCCATGCCGCGATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTg                                                                                                                                <  1:2932114/140‑1 (MQ=255)

        ttttCCATGCCGCGATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTc                                                                                                                              <  1:1813969/140‑1 (MQ=255)

           tCCATGCCGCGATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGcc                                                                                                                           <  1:2492763/140‑1 (MQ=255)

           tCCATGCCGCGATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGcc                                                                                                                           <  2:168437/140‑1 (MQ=255)

           tCCATGCCGCGATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGcc                                                                                                                           <  1:164854/140‑1 (MQ=255)

             cATGCCGCGATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCa                                                                                                                         <  1:3010340/140‑1 (MQ=255)

              aTGCCGCGATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAg                                                                                                                        >  1:1100416/1‑140 (MQ=255)

                  cgcgATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCg                                                                                                                    >  2:928593/1‑140 (MQ=255)

                    cgATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGCCCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGtt                                                                                                                  >  2:769421/1‑140 (MQ=255)

                    cgATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGtt                                                                                                                  >  2:1582229/1‑140 (MQ=255)

                    cgATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGtt                                                                                                                  >  2:2146986/1‑140 (MQ=255)

                       tGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTtgat                                                                                                               <  2:2056347/140‑1 (MQ=255)

                        ggACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTtgatg                                                                                                              <  1:3305502/140‑1 (MQ=255)

                         gACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTtgatga                                                                                                             >  2:1982881/1‑140 (MQ=255)

                           cGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAAc                                                                                                           <  1:714509/140‑1 (MQ=255)

                           cGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAAc                                                                                                           <  1:3282978/140‑1 (MQ=255)

                              aGAACAGACGTTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCg                                                                                                        <  2:3045414/140‑1 (MQ=255)

                              aGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCg                                                                                                        >  1:3483458/1‑140 (MQ=255)

                                 aCAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAg                                                                                                     <  2:2563981/140‑1 (MQ=255)

                                   aGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGt                                                                                                   <  1:1582229/140‑1 (MQ=255)

                                   aGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGt                                                                                                   >  1:2690276/1‑140 (MQ=255)

                                    gACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGtt                                                                                                  >  1:2637713/1‑140 (MQ=255)

                                     aCGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGttt                                                                                                 >  2:884119/1‑140 (MQ=255)

                                      cGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTc                                                                                                >  1:143110/1‑140 (MQ=255)

                                          aGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGt                                                                                                   <  1:1045800/133‑1 (MQ=255)

                                          aGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCa                                                                                            <  1:769421/140‑1 (MQ=255)

                                              ggCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCgg                                                                                        >  2:3102205/1‑140 (MQ=255)

                                                 gTCGATAATGTTTTCGCCATCGACGAATAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCggtgg                                                                                     >  2:180007/1‑140 (MQ=255)

                                                 gTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCggtgg                                                                                     >  2:2295561/1‑140 (MQ=255)

                                                 gTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCggtgg                                                                                     >  2:3326570/1‑140 (MQ=255)

                                                  tCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCggtggt                                                                                    <  2:2690276/140‑1 (MQ=255)

                                                  tCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCggtggt                                                                                    <  1:379976/140‑1 (MQ=255)

                                                  tCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCggtggt                                                                                    <  1:345363/140‑1 (MQ=255)

                                                   cGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAg                                                                                                     <  2:2467314/122‑1 (MQ=255)

                                                    gATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCg                                                                                  <  2:1932681/140‑1 (MQ=255)

                                                     aTAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCggtggt                                                                                    >  1:2654003/1‑137 (MQ=255)

                                                     aTAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCggtggt                                                                                    <  2:2654003/137‑1 (MQ=255)

                                                          gTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCg                                                                            <  1:674947/140‑1 (MQ=255)

                                                             ttCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCgg                                                                           <  1:3362319/138‑1 (MQ=255)

                                                             ttCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGAc                                                                         >  2:3124554/1‑140 (MQ=255)

                                                              tCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACg                                                                        <  1:2146986/140‑1 (MQ=255)

                                                              tCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACg                                                                        <  2:2139767/140‑1 (MQ=255)

                                                              tCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACg                                                                        <  1:2295561/140‑1 (MQ=255)

                                                              tCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACg                                                                        >  1:2625129/1‑140 (MQ=255)

                                                               cGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGc                                                                       <  1:3326570/140‑1 (MQ=255)

                                                               cGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGc                                                                       <  1:3102205/140‑1 (MQ=255)

                                                               cGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGc                                                                       <  2:2625129/140‑1 (MQ=255)

                                                               cGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGc                                                                       >  2:965881/1‑140 (MQ=255)

                                                                gCCATCGACGAATAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCt                                                                      <  1:180007/140‑1 (MQ=255)

                                                                gCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCt                                                                      <  2:3206281/140‑1 (MQ=255)

                                                                gCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCt                                                                      <  2:2862598/140‑1 (MQ=255)

                                                                    tCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACg                                                                  <  2:598701/140‑1 (MQ=255)

                                                                     cgacgaACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCt                                                                      <  1:1535034/135‑1 (MQ=255)

                                                                     cgacgaACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCt                                                                      >  2:1535034/1‑135 (MQ=255)

                                                                     cgacgaACAGACGGAAGTGGCCCGGTTCATCAGAAGTAACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTc                                                                                   <  1:67516/122‑1 (MQ=255)

                                                                      gacgaACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGAt                                                                <  2:1475188/140‑1 (MQ=255)

                                                                        cgaACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATaa                                                              <  2:661955/140‑1 (MQ=255)

                                                                         gaACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAAt                                                             <  2:1007590/140‑1 (MQ=255)

                                                                               aCGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGc                                                       <  2:1450881/140‑1 (MQ=255)

                                                                               aCGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGc                                                       <  2:1100416/140‑1 (MQ=255)

                                                                               aCGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGc                                                       <  2:243115/140‑1 (MQ=255)

                                                                               aCGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGc                                                       <  2:2502743/140‑1 (MQ=255)

                                                                               aCGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCACCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGc                                                       <  2:2529784/140‑1 (MQ=255)

                                                                                 ggAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTg                                                     >  1:1825040/1‑140 (MQ=255)

                                                                                    aGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCt                                                  >  2:1368397/1‑140 (MQ=255)

                                                                                     gTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCtt                                                 <  1:3124554/140‑1 (MQ=255)

                                                                                       ggCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTa                                               >  1:529324/1‑140 (MQ=255)

                                                                                       ggCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTa                                               >  1:266060/1‑140 (MQ=255)

                                                                                         cccGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACg                                             >  1:1280419/1‑140 (MQ=255)

                                                                                           cGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCa                                           <  2:3483458/140‑1 (MQ=255)

                                                                                           cGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCa                                           <  2:1280419/140‑1 (MQ=255)

                                                                                                  tcaGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTc                                                   <  2:3313027/125‑1 (MQ=255)

                                                                                                       aGTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGtt                               >  2:3208755/1‑140 (MQ=255)

                                                                                                        gTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTc                              >  1:1784402/1‑140 (MQ=255)

                                                                                                        gTGACGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTc                              >  1:275592/1‑140 (MQ=255)

                                                                                                           aCGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATc                           >  2:1101969/1‑140 (MQ=255)

                                                                                                           aCGTGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATc                           >  2:337722/1‑140 (MQ=255)

                                                                                                              tGCAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCgg                        >  2:544495/1‑140 (MQ=255)

                                                                                                                cAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGcc                      >  1:2310052/1‑140 (MQ=255)

                                                                                                                cAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGcc                      <  1:970925/140‑1 (MQ=255)

                                                                                                                cAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGcc                      <  1:825706/140‑1 (MQ=255)

                                                                                                                cAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGcc                      <  2:529324/140‑1 (MQ=255)

                                                                                                                cAGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGcc                      >  2:3287527/1‑140 (MQ=255)

                                                                                                                 aGCGGACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCa                     <  1:1553153/140‑1 (MQ=255)

                                                                                                                     gACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCa                 >  2:969729/1‑140 (MQ=255)

                                                                                                                     gACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCa                 <  1:1674264/140‑1 (MQ=255)

                                                                                                                     gACCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCa                 >  2:1867025/1‑140 (MQ=255)

                                                                                                                      aCCAATCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCAt                >  2:908209/1‑140 (MQ=255)

                                                                                                                          aTCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTTGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCATCCgg            >  2:3378055/1‑140 (MQ=255)

                                                                                                                          aTCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCATCCgg            <  1:3208755/140‑1 (MQ=255)

                                                                                                                          aTCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCATCCgg            >  2:1380558/1‑140 (MQ=255)

                                                                                                                          aTCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCATCCgg            <  2:1784402/140‑1 (MQ=255)

                                                                                                                           tCGGCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCATCCGgc           >  2:2322063/1‑140 (MQ=255)

                                                                                                                              gCACGACGTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCATCCGgcagc        <  2:2942114/140‑1 (MQ=255)

                                                                                                                                acgacgTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCATCCGGCAGCAc      <  1:320593/140‑1 (MQ=255)

                                                                                                                                  gacgTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCATCCGGCAGCACCa    >  1:1445898/1‑140 (MQ=255)

                                                                                                                                    cgTTGCTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCATCCGGCAGCACCAga  >  1:2376198/1‑140 (MQ=255)

                                                                                                                                     gttgctTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGt                   <  1:2514908/122‑1 (MQ=255)

CCGCCAGTTTTTCCATGCCGCGATGGACGTAGAACAGACGGTAGTCGGCGTCGATAATGTTTTCGCCATCGACGAACAGACGGAAGTGGCCCGGTTCATCAGAAGTGACGTGCAGCGGACCAATCGGCACGACGTTGTTTTTCTTGTCGCCCAGTTCGTTGATGAACTCGTAGGTTTCAGCATCGGTGGTCGGTGCCGGACGCTGACGATAATCCATGCTGTCTTTACGCAGCGGATAAAGTTCATCCGGCCAGTCATCCGGCAGCACCAGA  >  NC_000913/2845799‑2846070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`