BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,465,428	G→A	R116H (CGC→CAC)	gspM →	Type II secretion system protein GspM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,465,428	0	G	A	100.0%	845.8 / NA	234	R116H (CGC→CAC)	gspM	Type II secretion system protein GspM
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (86/148); total (86/148)

Only 100 of 237 total aligned reads displayed.

TCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCT  >  NC_000913/3465291‑3465562

tCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACt                                                                                                                                      >  2:1922787/1‑140 (MQ=255)

  cTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTgg                                                                                                                                    <  2:224877/140‑1 (MQ=255)

  cTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTgg                                                                                                                                    >  1:2354673/1‑140 (MQ=255)

    gcgcTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCt                                                                                                                                  <  1:895240/140‑1 (MQ=255)

     cgcTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTc                                                                                                                                 >  1:2826346/1‑140 (MQ=255)

      gcTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCa                                                                                                                                <  1:1214235/140‑1 (MQ=255)

         tACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTa                                                                                                                             >  2:2338759/1‑140 (MQ=255)

          aCGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTAc                                                                                                                            >  2:1596828/1‑140 (MQ=255)

           cGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACg                                                                                                                           >  2:1452694/1‑140 (MQ=255)

             cAGCCGATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGc                                                                                                                         <  1:1742709/140‑1 (MQ=255)

              aGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGca                                                                                                                        <  2:945475/140‑1 (MQ=255)

               gCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGcac                                                                                                                       <  1:2882285/140‑1 (MQ=255)

               gCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGcac                                                                                                                       <  2:3319635/140‑1 (MQ=255)

               gCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGcac                                                                                                                       <  1:2609494/140‑1 (MQ=255)

               gCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGcac                                                                                                                       >  1:3319635/1‑140 (MQ=255)

               gCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGcac                                                                                                                       <  2:1644266/140‑1 (MQ=255)

               gCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGcac                                                                                                                       <  2:3457702/140‑1 (MQ=255)

                cccATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGcaca                                                                                                                      >  2:2022138/1‑140 (MQ=255)

                cccATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGc                                                                                                                         >  1:2157587/1‑137 (MQ=255)

                cccATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGc                                                                                                                         <  2:2157587/137‑1 (MQ=255)

                 ccATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACgg                                                                                                                          <  2:2047187/135‑1 (MQ=255)

                 ccATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAg                                                                                                                     <  1:2022138/140‑1 (MQ=255)

                  cATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGTCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAgg                                                                                                                    <  1:495493/140‑1 (MQ=255)

                  cATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGca                                                                                                                        >  1:2176638/1‑136 (MQ=255)

                  cATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGca                                                                                                                        <  2:2176638/136‑1 (MQ=255)

                  cATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAgg                                                                                                                    <  2:458851/140‑1 (MQ=255)

                  cATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAgg                                                                                                                    <  1:2233744/140‑1 (MQ=255)

                  cATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAgg                                                                                                                    <  2:2543747/140‑1 (MQ=255)

                  cATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAgg                                                                                                                    <  1:750767/140‑1 (MQ=255)

                    tCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGcac                                                                                                                       >  1:777084/1‑135 (MQ=255)

                    tCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTa                                                                                                                  <  1:1551485/140‑1 (MQ=255)

                         aaTATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATa                                                                                                             <  1:3486372/140‑1 (MQ=255)

                          atatCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAgg                                                                                                                    >  1:5306/1‑132 (MQ=255)

                          atatCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAgg                                                                                                                    <  2:5306/132‑1 (MQ=255)

                          atatCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATAc                                                                                                            >  2:793481/1‑140 (MQ=255)

                           tatCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACg                                                                                                           <  1:2539608/140‑1 (MQ=255)

                             tCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATAc                                                                                                            <  1:3392079/137‑1 (MQ=255)

                             tCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATAc                                                                                                            >  2:3392079/1‑137 (MQ=255)

                              ccTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTa                                                                                                        <  2:620095/140‑1 (MQ=255)

                               cTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTAt                                                                                                       <  2:2783407/140‑1 (MQ=255)

                               cTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACAGCACAGGTAACATACGGAAt                                                                                                       >  2:1014086/1‑140 (MQ=255)

                                          aGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAg                                                                                            <  2:2473687/140‑1 (MQ=255)

                                          aGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAg                                                                                            >  1:1378818/1‑140 (MQ=255)

                                          aGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAg                                                                                            <  1:196018/140‑1 (MQ=255)

                                              aaaCGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCt                                                                                        <  2:1793799/140‑1 (MQ=255)

                                              aaaCGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCt                                                                                        <  1:1922787/140‑1 (MQ=255)

                                                 cGTGAGAATTTGGTTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCa                                                                                     >  1:2812901/1‑140 (MQ=255)

                                                      gaATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGAc                                                                                          >  1:2857059/1‑130 (MQ=255)

                                                      gaATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGAc                                                                                          >  1:2296644/1‑130 (MQ=255)

                                                      gaATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGAc                                                                                          <  2:2857059/130‑1 (MQ=255)

                                                                 tCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGa                                                                     <  1:1452694/140‑1 (MQ=255)

                                                                 tCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGa                                                                     >  1:911756/1‑140 (MQ=255)

                                                                       tGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGaa                                                               <  1:626365/140‑1 (MQ=255)

                                                                       tGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGaa                                                               <  1:2430856/140‑1 (MQ=255)

                                                                            aaCGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGAt                                                          <  1:1596828/140‑1 (MQ=255)

                                                                               ggCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGG‑AACGAAGAGATCAcc                                                      >  1:401694/1‑140 (MQ=255)

                                                                                 cccGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCAccc                                                     >  1:650055/1‑140 (MQ=255)

                                                                                 cccGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCAccc                                                     >  2:1522999/1‑140 (MQ=255)

                                                                                 cccGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCAccc                                                     >  2:486208/1‑140 (MQ=255)

                                                                                      aCAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAgg                                                <  1:2046416/140‑1 (MQ=255)

                                                                                       cAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGc                                               <  2:650055/140‑1 (MQ=255)

                                                                                       cAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGc                                               <  1:1025456/140‑1 (MQ=255)

                                                                                           aCTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCAttt                                           <  2:1187997/140‑1 (MQ=255)

                                                                                               tGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCAt                                             <  2:2440527/134‑1 (MQ=255)

                                                                                               tGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTcc                                       >  2:1445229/1‑140 (MQ=255)

                                                                                                gACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCt                                      >  2:2682778/1‑140 (MQ=255)

                                                                                                    aTTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTc                                        >  1:2982417/1‑134 (MQ=255)

                                                                                                    aTTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAg                                  >  2:675706/1‑140 (MQ=255)

                                                                                                    aTTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAg                                  >  1:764779/1‑140 (MQ=255)

                                                                                                     ttCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAgg                                 >  2:1703149/1‑140 (MQ=255)

                                                                                                      tCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAggg                                <  1:941466/140‑1 (MQ=255)

                                                                                                       cATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGG‑AACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAg                              <  2:401694/140‑1 (MQ=255)

                                                                                                        aTCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAg                              <  1:1042607/140‑1 (MQ=255)

                                                                                                        aTCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAg                              <  2:764779/140‑1 (MQ=255)

                                                                                                        aTCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAg                              <  1:2338759/140‑1 (MQ=255)

                                                                                                         tCCTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGc                             >  2:10798/1‑140 (MQ=255)

                                                                                                          ccTGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGca                            <  2:2992984/140‑1 (MQ=255)

                                                                                                            tGTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGcaac                          <  1:176610/140‑1 (MQ=255)

                                                                                                             gTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCa                                   <  1:3333277/130‑1 (MQ=255)

                                                                                                             gTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCa                                   >  2:3333277/1‑130 (MQ=255)

                                                                                                             gTCACAGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGcaaca                         >  1:1285836/1‑140 (MQ=255)

                                                                                                               cacaGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACaat                       <  1:675706/140‑1 (MQ=255)

                                                                                                               cacaGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACaat                       >  1:2106521/1‑140 (MQ=255)

                                                                                                                acaGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAggg                                >  2:3194744/1‑130 (MQ=255)

                                                                                                                acaGCTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACaata                      <  2:911756/140‑1 (MQ=255)

                                                                                                                    cTCCCCTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGca                            <  1:2647084/130‑1 (MQ=255)

                                                                                                                       cccTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGca                            >  2:287052/1‑127 (MQ=255)

                                                                                                                       cccTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACaa               >  1:3097587/1‑140 (MQ=255)

                                                                                                                       cccTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACaa               >  1:2450174/1‑140 (MQ=255)

                                                                                                                       cccTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACaa               >  1:135954/1‑140 (MQ=255)

                                                                                                                        ccTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACaa               >  1:365865/1‑139 (MQ=255)

                                                                                                                        ccTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACaa               <  2:365865/139‑1 (MQ=255)

                                                                                                                        ccTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAAt              >  1:1431483/1‑140 (MQ=255)

                                                                                                                        ccTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAAt              >  1:2949249/1‑140 (MQ=255)

                                                                                                                         cTCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATg             >  2:1324673/1‑140 (MQ=255)

                                                                                                                          tCGAAAACGTCTCCTACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGc            >  1:1725765/1‑140 (MQ=255)

                                                                                                                          tCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGc            <  2:1285836/140‑1 (MQ=255)

                                                                                                                          tCGAAAACGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGc            <  1:1445229/140‑1 (MQ=255)

                                                                                                                                 cGTCTCCTACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGcc     <  2:1725765/140‑1 (MQ=255)

                                                                                                                                 cGTCTCCCACTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGcc     >  1:3192649/1‑140 (MQ=255)

TCCTGCGCTTACGCAGCCCATCAAAAATATCCTTCTCGAGGAAGCAAAACGTGAGAATTTGGCTATCACGCTGGAAAACGGCCCGGACAATACTCTGACAATTCATCCTGTCACAGCTCCCCTCGAAAACGTCTCCCGCTGGCTCACTACGGCACAGGTAACATACGGTATTGTTATTGAAGACCTTCAGTTTACTCTTGCCGGAAACGAAGAGATCACCCTCAGGCATTTGTCCTTCAGGGAGCAACAATAATGACAATGCTACTGCCGCT  >  NC_000913/3465291‑3465562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`