BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,525,052	T→C	F728L (TTC→CTC)	mrcA →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,525,052	0	T	C	100.0%	463.2 / NA	129	F728L (TTC→CTC)	mrcA	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/101); total (28/101)

Only 100 of 165 total aligned reads displayed.

GATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGC  >  NC_000913/3524916‑3525188

 aTTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCtct‑c                                                                                                                                      <  1:415374/140‑1 (MQ=255)

 aTTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCtct‑c                                                                                                                                      <  1:1072614/140‑1 (MQ=255)

  ttAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGGAAAACCGGACCACCAACAAGTCGAAAAATGCGTGGGTCT‑Cg                                                                                                                                     >  2:2435889/1‑140 (MQ=255)

      gagTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACCGGGCCACCAACAATTCGAAAGATGCGGGGGTCT‑Cggggt                                                                                                                                 >  1:100961/1‑138 (MQ=255)

       agTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTa                                                                                                                                <  1:2157313/140‑1 (MQ=255)

       agTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTa                                                                                                                                <  1:1213541/140‑1 (MQ=255)

       agTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACGGGGCCACCAACAATTCGAAAAAAGCGTGGGTCT‑Cggggtt                                                                                                                                >  1:39250/1‑137 (MQ=255)

        gTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGGAAAACCGGG‑CCCCCAAAAATTCGAAAAAAGCGGGGGTCTCCGGGttg                                                                                                                                >  1:132121/1‑139 (MQ=255)

        gTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACCGGGCCCCCAAAAATTCGAAAAAAGCGGGGGTCT‑Cgggggtc                                                                                                                               >  2:3301863/1‑136 (MQ=255)

           cTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACGGGGACACTAACAATTCGAAAGAAGCGGGGCTCT‑Cggggtggggt                                                                                                                            >  2:343532/1‑133 (MQ=255)

           agttGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGt                                                                                                                            <  1:17681/138‑1 (MQ=255)

              tGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGGAAAAAGGGGCCCCCCACAATTTGAAAGAAGCGGGGGTCT‑CGGGGTACGGTGCg                                                                                                                         >  2:941932/1‑140 (MQ=255)

                       atatCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACGGGGGCACCAACAAGTCGAAAAAAGCGGGGGCCT‑CGGGGTACGGTTCGGGCGGtgtg                                                                                                                >  2:1194024/1‑140 (MQ=255)

                       atatCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACCGGGCCACCAACAAATCGGAAGAAGGGTGGGTCT‑CCGGGTTCGGGCCGGGCGGtggg                                                                                                                >  2:2041041/1‑138 (MQ=255)

                        tatCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGGAAAACGGGAACACCAAAAACTCGAAAAAAGCGGGGGTCC‑CGGGGTACGGGCCCGGCGGtgtgg                                                                                                               >  2:2849654/1‑139 (MQ=255)

                        tatCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGa                                                                                                               <  2:2864935/140‑1 (MQ=255)

                          tCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGAcc                                                                                                             <  2:1488289/140‑1 (MQ=255)

                           cTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCt                                                                                                            <  1:809100/140‑1 (MQ=255)

                            tttGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTc                                                                                                           <  2:3090182/140‑1 (MQ=255)

                             ttGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGGAAAACCGGGCCCCCAAAAAATT‑GAAAAAAGCGGGGGGCT‑CGGGGTAAGGGCCGGGGGGtgtgggcccg                                                                                                          >  2:837932/1‑134 (MQ=255)

                              tGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCgg                                                                                                         <  1:852124/140‑1 (MQ=255)

                               ggTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGGAAAACCGGG‑GCACCAACAAGTTGAAAAAAGCGCGGGGCG‑CGGGGTACGGTCCGGGCGGTGTGGGCTCggg                                                                                                        >  2:1323520/1‑139 (MQ=255)

                               ggTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGt                                                                                                        <  1:2664677/140‑1 (MQ=255)

                                  gAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGGAAAACGGGGCCACCAACAATTCGAAAAAAGCGGGGGTCT‑CGGGGTTCGGGCCCGGCGGTGTGGCCCCGGGCTg                                                                                                     >  2:3087118/1‑140 (MQ=255)

                                   aGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGGAAAACGGGGCCCCCAAAAGATTCAAAAAAGGGTGGGTCT‑CCGGGTTCGGGCCGGGGGGTGTGGGCTCGGGCttg                                                                                                    >  2:33348/1‑138 (MQ=255)

                                    gCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGGAAACCCGGGCCCCCAACAATTCGAAAAATGCGGGGCGCT‑CGGGGTACCGTGCGGGCGGTGTGGCCTCGGTCTggg                                                                                                   >  2:2500181/1‑139 (MQ=255)

                                    gCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACAGGGCCCCCAAAAAGTCGAAAAAAGCGGGGGGCT‑CCGGGTACGGGCCCGGCGGTGTGGCCTCGGGCTggg                                                                                                   >  2:1977668/1‑139 (MQ=255)

                                     ccAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGGAAAACCGGCGCC‑CCCACAGATGCACAGATAGGGGGGTCG‑CGGGGTACGGGCCGGGGGTTGTGGCCCCCGGCTgggg                                                                                                  >  2:199513/1‑138 (MQ=255)

                                     ccAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGAt                                                                                                  <  1:2041041/140‑1 (MQ=255)

                                        ggCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGGAAAAACGGG‑CCCCCAAAAGATTGAAAAATGCGGGGCGCT‑CGGGGTTCGGGCCGGGCGGTGTGGCCCCGGGCTGGGtttg                                                                                               >  2:2581885/1‑138 (MQ=255)

                                        ggCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTgg                                                                                               <  1:33348/140‑1 (MQ=255)

                                         gCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACCGGAGCCCCCACAAATTGAAAAAAGCGGGGGTCT‑CGGGGTTCGGGCCGGGCGGTGTGGCCTCCGGCTGGATTggg                                                                                              >  2:2750969/1‑139 (MQ=255)

                                          cTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCt                                                                                             <  1:1031704/140‑1 (MQ=255)

                                          cTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCt                                                                                             <  1:1613273/140‑1 (MQ=255)

                                           tGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCtt                                                                                            <  1:8791/140‑1 (MQ=255)

                                             gCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTtg                                                                                          <  1:251801/140‑1 (MQ=255)

                                             gCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTtg                                                                                          <  1:1279585/140‑1 (MQ=255)

                                                    aCTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCa                                                                                   <  1:533419/140‑1 (MQ=255)

                                                     ctggctggCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCAc                                                                                  <  1:3272672/140‑1 (MQ=255)

                                                     ctggctggCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCAc                                                                                  <  1:1489220/140‑1 (MQ=255)

                                                     ctggctggCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACCGGGGCACCCAAAGATCGAAAAAAGCGGGGGGCT‑CGGGGTACGGGCCGGGCGGTGTGGCCTCGGGCTGGGTTGGCTTTGTTGATCAc                                                                                  >  2:155935/1‑140 (MQ=255)

                                                         ctggcGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACcgtc                                                                              <  2:445133/140‑1 (MQ=255)

                                                          tggcGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACcgtcg                                                                             <  1:1543322/140‑1 (MQ=255)

                                                            gcGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCCCCAAAAGTTCGAAAAAGGCGGGGGCCT‑CCGGTTACGGGCCGGGCGGGGGGGCCCCGGGCTGGGTTGGGTTTGTTGATCAccccccta                                                                           >  2:1736615/1‑134 (MQ=255)

                                                            gcGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTa                                                                           <  1:2119210/140‑1 (MQ=255)

                                                             cGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGGAAAACCGGG‑CCCCTAACAAGTCCAAAGAGGAGGGGGTCT‑CCGGGTTCGGGCGGGGGGGTGTGGACTCGGGCTGGGTTGGCTTTTGTGATCACCCTCGGaa                                                                          >  2:1128176/1‑140 (MQ=255)

                                                                gCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAAtct                                                                       <  2:2935131/140‑1 (MQ=255)

                                                                gCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAAtct                                                                       <  1:2056443/140‑1 (MQ=255)

                                                                gCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAAtct                                                                       <  1:2771791/140‑1 (MQ=255)

                                                                 cAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAAtctc                                                                      <  2:1400198/140‑1 (MQ=255)

                                                                  aGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCg                                                                     <  1:1603146/140‑1 (MQ=255)

                                                                   ggTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCgg                                                                    <  2:3206968/140‑1 (MQ=255)

                                                                    gTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGt                                                                   <  1:3275450/140‑1 (MQ=255)

                                                                    gTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGt                                                                   <  2:87352/140‑1 (MQ=255)

                                                                      cGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAAACCGGGCCCCCCACAGATCGAAAAAAGCGGGGGGCT‑CGGGGTACGGTCCCGGCGGTGTGGCCCCGGGCTGGATTGGGCTTGATGATCACCCCCGGAAACTCGGGCa                                                                 >  2:2270095/1‑140 (MQ=255)

                                                                        tGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATa                                                               <  1:175132/140‑1 (MQ=255)

                                                                          aTTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACa                                                             <  2:100961/140‑1 (MQ=255)

                                                                            ttGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAAc                                                           <  1:1977668/140‑1 (MQ=255)

                                                                            ttGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAAc                                                           <  1:2548618/140‑1 (MQ=255)

                                                                            ttGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAAc                                                           <  1:1471744/140‑1 (MQ=255)

                                                                                       cgATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAg                                                <  2:39250/140‑1 (MQ=255)

                                                                                        gATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGc                                               <  1:1356894/140‑1 (MQ=255)

                                                                                          tatCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGa                                             <  1:3087118/140‑1 (MQ=255)

                                                                                           atCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGAt                                            <  1:343532/140‑1 (MQ=255)

                                                                                             cggc‑ggGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTa                                          <  1:3168017/140‑1 (MQ=255)

                                                                                               gc‑ggGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTaaa                                        <  1:2592119/140‑1 (MQ=255)

                                                                                                  gggAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAaga                                      <  2:1941132/140‑1 (MQ=255)

                                                                                                   ggAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAagat                                     <  2:636497/140‑1 (MQ=255)

                                                                                                        aCCGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAagatcagat                                <  1:914524/140‑1 (MQ=255)

                                                                                                         ccGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAagatcagatc                               <  2:636295/140‑1 (MQ=255)

                                                                                                          cGGGACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGAtct                              <  1:1906613/140‑1 (MQ=255)

                                                                                                           gggACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGAtctc                             <  1:604417/140‑1 (MQ=255)

                                                                                                           gggACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGAtctc                             >  1:700267/1‑140 (MQ=255)

                                                                                                            ggACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCa                            >  1:1585240/1‑140 (MQ=255)

                                                                                                            ggACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCa                            <  1:2487159/140‑1 (MQ=255)

                                                                                                             gACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAg                           <  2:2476978/140‑1 (MQ=255)

                                                                                                             gACCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAg                           <  1:2860672/140‑1 (MQ=255)

                                                                                                              aCCACTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAgg                          <  1:341504/140‑1 (MQ=255)

                                                                                                                  cTAACAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTAc                      >  1:189417/1‑140 (MQ=255)

                                                                                                                    aaCAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAg                           <  2:2129573/133‑1 (MQ=255)

                                                                                                                      cAGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAg                  >  2:3160811/1‑140 (MQ=255)

                                                                                                                       aGTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAgg                 >  1:3059297/1‑140 (MQ=255)

                                                                                                                        gTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGc                >  2:2815421/1‑140 (MQ=255)

                                                                                                                        gTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGc                <  1:837932/140‑1 (MQ=255)

                                                                                                                        gTTCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGc                <  1:2116831/140‑1 (MQ=255)

                                                                                                                          tCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGc                >  1:1800362/1‑138 (MQ=255)

                                                                                                                          tCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGc                <  2:1800362/138‑1 (MQ=255)

                                                                                                                          tCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCgg              >  2:1573420/1‑140 (MQ=255)

                                                                                                                          tCGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCgg              >  2:457058/1‑140 (MQ=255)

                                                                                                                           cGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACACCGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGt             >  1:2314181/1‑140 (MQ=255)

                                                                                                                           cGAAAGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGt             >  2:1750718/1‑140 (MQ=255)

                                                                                                                             aaaGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGc                <  2:2399626/135‑1 (MQ=255)

                                                                                                                             aaaGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGc                >  1:2399626/1‑135 (MQ=255)

                                                                                                                             aaaGATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGc           <  1:1128176/140‑1 (MQ=255)

                                                                                                                                gATGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCaa        <  2:189417/140‑1 (MQ=255)

                                                                                                                                 aTGCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAag       >  2:314260/1‑140 (MQ=255)

                                                                                                                                   gCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAagag     >  2:1641519/1‑140 (MQ=255)

                                                                                                                                   gCGTGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAagag     >  1:946558/1‑140 (MQ=255)

                                                                                                                                      tGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGc  <  2:700267/140‑1 (MQ=255)

                                                                                                                                      tGGCTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGc  <  2:2191388/140‑1 (MQ=255)

GATTAAGAGTGCTTTGAACACCAATATCTTTGGTGAGCCAGGCTGGCAGGGTACTGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGC‑GGGAAAACCGGGACCACTAACAGTTCGAAAGATGCGTGGTTCT‑CGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCGTCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGC  >  NC_000913/3524916‑3525188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`