Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	75,380	(C)5→4	coding (101/984 nt)	thiB ←	thiamine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	75,376	0	C	.	100.0%	274.9 / NA	65	coding (105/984 nt)	thiB	thiamine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (28/37);  total (28/37)

GCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGAC  >  NC_000913/75243‑75510                                                                                                                                      |                                                                                                                                       gCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGccccagt                                                                                                                                  <  1:883120/140‑4 (MQ=255)  caccacATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGccccagtc                                                                                                                                 >  2:569496/1‑136 (MQ=255)    ccacATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTcgg                                                                                                                              <  2:561006/140‑1 (MQ=255)      acaTCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTcggcg                                                                                                                            >  1:7573/1‑140 (MQ=255)       caTCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTcggcgg                                                                                                                           >  1:519906/1‑140 (MQ=255)       caTCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTcggcgg                                                                                                                           >  2:883692/1‑140 (MQ=255)            gCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGa                                                                                                                        >  2:759865/1‑138 (MQ=255)            gCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGa                                                                                                                        <  1:759865/138‑1 (MQ=255)            gCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAg                                                                                                                      <  1:347381/140‑1 (MQ=255)            gCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAg                                                                                                                      <  2:285562/140‑1 (MQ=255)                tACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAAt                                                                                                                  <  2:569596/140‑1 (MQ=255)                tACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAAt                                                                                                                  <  1:790079/140‑1 (MQ=255)                  cTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCg                                                                                                                <  1:883692/140‑1 (MQ=255)                  cTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCg                                                                                                                <  2:666486/140‑1 (MQ=255)                    gTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTa                                                                                                              <  1:842037/140‑1 (MQ=255)                    gTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTa                                                                                                              <  2:1271111/140‑1 (MQ=255)                       tttGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGt                                                                                                           >  1:536016/1‑140 (MQ=255)                          gCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGtata                                                                                                        <  1:971969/140‑1 (MQ=255)                           ccTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATaa                                                                                                       <  2:7573/140‑1 (MQ=255)                               ccATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAgg                                                                                                            >  1:496692/1‑131 (MQ=255)                               ccATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAgg                                                                                                            <  2:496692/131‑1 (MQ=255)                               ccATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAg                                                                                                   >  1:803209/1‑140 (MQ=255)                                 aTCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTc                                                                                                 <  2:312504/140‑1 (MQ=255)                                 aTCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTc                                                                                                 <  2:791582/140‑1 (MQ=255)                                 aTCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTc                                                                                                 <  2:1117036/140‑1 (MQ=255)                                        gACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACgg                                                                                          >  1:235497/1‑140 (MQ=255)                                            gTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGttt                                                                                      >  1:1157683/1‑140 (MQ=255)                                             ttGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTa                                                                                     >  1:851997/1‑140 (MQ=255)                                             ttGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTa                                                                                     >  1:968184/1‑140 (MQ=255)                                             ttGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTa                                                                                     >  2:1234868/1‑140 (MQ=255)                                                agaCGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCg                                                                                  >  1:364029/1‑140 (MQ=255)                                                agaAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCg                                                                                  >  2:795231/1‑140 (MQ=255)                                                       aaaCGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGaa                                                                                             >  1:1183619/1‑122 (MQ=255)                                                       aaaCGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGaa                                                                                             <  2:1183619/122‑1 (MQ=255)                                                       aaaCGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCg                                                                                  >  1:473300/1‑133 (MQ=255)                                                       aaaCGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCg                                                                                  <  2:473300/133‑1 (MQ=255)                                                       aaaCGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACgg                                                                           >  2:1235215/1‑140 (MQ=255)                                                       aaaCGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACgg                                                                           <  1:1234868/140‑1 (MQ=255)                                                       aaaCGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACgg                                                                           >  1:1327036/1‑140 (MQ=255)                                                       aaaCGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACgg                                                                           >  1:251592/1‑140 (MQ=255)                                                       aaaCGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACgg                                                                           <  1:795231/140‑1 (MQ=255)                                                       aaaCGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACgg                                                                           <  2:364029/140‑1 (MQ=255)                                                          cGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGgcg                                                                        <  2:1157683/140‑1 (MQ=255)                                                                 ttCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGca                                                                 <  2:968184/140‑1 (MQ=255)                                                                     aGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCacagc                                                             <  2:235497/140‑1 (MQ=255)                                                                     aGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCacagc                                                             <  1:569496/140‑1 (MQ=255)                                                                      gcgcCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCacagca                                                            <  1:1235215/140‑1 (MQ=255)                                                                         ccaccaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCacagcaaca                                                         >  2:756433/1‑140 (MQ=255)                                                                           accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGgcg                                                                        <  2:524050/123‑1 (MQ=255)                                                                           accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGgcg                                                                        >  1:524050/1‑123 (MQ=255)                                                                           accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCacagcaacagc                                                       <  2:536016/140‑1 (MQ=255)                                                                                    aGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGa                                              <  1:472673/140‑1 (MQ=255)                                                                                     gTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGAc                                             <  2:519906/140‑1 (MQ=255)                                                                                     gTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGAc                                             <  2:1327036/140‑1 (MQ=255)                                                                                       tCGCCAATACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTACACAGCAACAGCAGGGGCAGACAt                                           <  2:741164/140‑1 (MQ=255)                                                                                        cGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACAtt                                          >  1:344541/1‑140 (MQ=255)                                                                                          cAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACAtttt                                        >  2:586070/1‑140 (MQ=255)                                                                                          cAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACAtttt                                        >  2:1297330/1‑140 (MQ=255)                                                                                              tACAGTCGGCTTCAAAGGCTTTTTTAACCCCCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTa                                    <  2:920693/140‑1 (MQ=255)                                                                                                 aGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAaca                                 <  2:903663/140‑1 (MQ=255)                                                                                                       cTTCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGc                           >  1:61792/1‑140 (MQ=255)                                                                                                        ttCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                          <  2:251592/140‑1 (MQ=255)                                                                                                        ttCAAAGGCTTTTTTAACCACCGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                          >  2:1350488/1‑140 (MQ=255)                                                                                                                          caccGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCa        <  2:1033861/140‑1 (MQ=255)                                                                                                                           accGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa       <  1:405023/140‑1 (MQ=255)                                                                                                                           accGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa       >  2:405023/1‑140 (MQ=255)                                                                                                                             cGGACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAg     >  1:919343/1‑140 (MQ=255)                                                                                                                               gACCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGa   <  2:851997/140‑1 (MQ=255)                                                                                                                                aCCAGG‑CCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGAc  <  2:344541/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       GCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGAC  >  NC_000913/75243‑75510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑