Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,116,428	(T)6→7	coding (700/1221 nt)	wcaL ←	putative colanic biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,116,422	1	.	T	100.0%	222.9 / NA	63	G236S (GGC→AGC)	wcaL	putative colanic biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (35/28);  total (35/28)

ACATCTTCCAGTTGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCC‑TTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTCATATCTACGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGCAG  >  NC_000913/2116289‑2116549                                                                                                                                       |                                                                                                                                acaTCTTCCAGTTGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCC‑tttttt                                                                                                                           <  2:184566/140‑1 (MQ=255)   aTCTTCCAGTTGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCC‑tttttttc                                                                                                                         >  1:447457/1‑138 (MQ=255)    tCTTCCAGTTGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCC‑tttttttct                                                                                                                        <  2:1073909/140‑4 (MQ=255)    tCTTCCAGTTGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCC‑tttttttct                                                                                                                        >  1:151466/1‑137 (MQ=255)    tCTTCCAGTTGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCC‑tttttttct                                                                                                                        <  2:1346368/140‑4 (MQ=255)       tCCAGTTGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCgg                                                                                                                      >  1:595373/1‑140 (MQ=255)        ccAGTTGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGt                                                                                                                     <  1:1060462/140‑1 (MQ=255)          aGTTGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCATGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCC‑tttt                                                                                                                             <  1:159518/129‑1 (MQ=255)          aGTTGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCATGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCC‑tttt                                                                                                                             >  2:159518/1‑129 (MQ=255)            ttGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAg                                                                                                                 >  2:634859/1‑140 (MQ=255)              gATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCg                                                                                                               >  2:898137/1‑140 (MQ=255)               atatTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGt                                                                                                              >  1:519455/1‑140 (MQ=255)                tatTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGt                                                                                                              >  1:456484/1‑139 (MQ=255)                tatTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGt                                                                                                              <  2:456484/139‑1 (MQ=255)                tatTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTg                                                                                                             >  2:1029791/1‑140 (MQ=255)                 atTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGc                                                                                                            >  2:641846/1‑140 (MQ=255)                   tGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGa                                                                                                          <  1:634859/140‑1 (MQ=255)                   tGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGa                                                                                                          <  1:1029791/140‑1 (MQ=255)                   tGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGa                                                                                                          <  2:151466/140‑1 (MQ=255)                     ttCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACg                                                                                                        >  2:738926/1‑140 (MQ=255)                     ttCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACg                                                                                                        >  1:416133/1‑140 (MQ=255)                     ttCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACg                                                                                                        >  2:176105/1‑140 (MQ=255)                     ttCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACg                                                                                                        >  2:678280/1‑140 (MQ=255)                      tCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACgg                                                                                                       <  1:525743/140‑1 (MQ=255)                          tGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTg                                                                                                             >  1:468786/1‑130 (MQ=255)                          tGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTg                                                                                                             <  2:468786/130‑1 (MQ=255)                          tGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAaat                                                                                                   >  2:230685/1‑140 (MQ=255)                              ggTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACgg                                                                                                       <  2:1194317/132‑1 (MQ=255)                              ggTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACgg                                                                                                       >  1:1194317/1‑132 (MQ=255)                               gTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAAtct                                                                                              >  2:564046/1‑140 (MQ=255)                               gTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAAtct                                                                                              >  1:384494/1‑140 (MQ=255)                                     aGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAgcg                                                                                        <  2:77201/140‑1 (MQ=255)                                       gCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAgcggc                                                                                      >  1:955973/1‑140 (MQ=255)                                          tCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGtt                                                                                   >  1:799353/1‑140 (MQ=255)                                            tttCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGc                                                                                 >  1:596519/1‑140 (MQ=255)                                                ccACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGgc                                                                             <  1:176105/140‑1 (MQ=255)                                                 cACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGgcg                                                                            <  2:416133/140‑1 (MQ=255)                                                     ggCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCttt                                                                        >  2:362331/1‑140 (MQ=255)                                                      gCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTc                                                                       >  2:1172704/1‑140 (MQ=255)                                                             ccGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGAc                                                                <  2:447457/140‑1 (MQ=255)                                                             ccGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGAc                                                                <  2:384494/140‑1 (MQ=255)                                                                aGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGTCGTTGCGGGCGCTTTCACGGGAcgcg                                                             >  1:26365/1‑140 (MQ=255)                                                                aGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGAcgcg                                                             >  2:504435/1‑140 (MQ=255)                                                                aGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGAc                                                                <  2:716482/137‑1 (MQ=255)                                                                aGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGAc                                                                >  1:716482/1‑137 (MQ=255)                                                                        aTAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAg                                                     >  2:360901/1‑140 (MQ=255)                                                                        aTAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAg                                                     <  1:724156/140‑1 (MQ=255)                                                                         tAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAgc                                                    <  1:230685/140‑1 (MQ=255)                                                                         tAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAgc                                                    <  1:405018/140‑1 (MQ=255)                                                                            cGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGcgcg                                                 >  2:189649/1‑140 (MQ=255)                                                                            cGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGcgcg                                                 >  1:1333218/1‑140 (MQ=255)                                                                            cGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGcgcg                                                 >  1:924274/1‑140 (MQ=255)                                                                            cGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTAAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGcgcg                                                 <  2:1112754/140‑1 (MQ=255)                                                                              aaaTGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTc                                               <  2:634519/140‑1 (MQ=255)                                                                              aaaTGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTc                                               <  2:1333218/140‑1 (MQ=255)                                                                              aaaTGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTc                                               <  2:450899/140‑1 (MQ=255)                                                                              aaaTGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTc                                               <  1:898137/140‑1 (MQ=255)                                                                                     aCGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTCATATCTa                                        <  1:360901/140‑1 (MQ=255)                                                                                         ccTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTCATATCTACGcc                                    >  2:1144190/1‑140 (MQ=255)                                                                                          cTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTCATATCTACGccc                                   <  1:189649/140‑1 (MQ=255)                                                                                           tGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGt                                                <  1:983976/126‑1 (MQ=255)                                                                                           tGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGt                                                >  2:983976/1‑126 (MQ=255)                                                                                            gCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAgcg                                                   <  2:1286806/122‑1 (MQ=255)                                                                                            gCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAgcg                                                   >  1:1286806/1‑122 (MQ=255)                                                                                             ctctTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGTCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTCATATCTACGCCCATg                                <  2:26365/140‑1 (MQ=255)                                                                                                  tCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTCATATCTACGCCCATGCGCGa                           >  1:1004369/1‑140 (MQ=255)                                                                                                        gACGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTCATATCTACGCCCATGCGCGATACGGc                     <  1:504435/140‑1 (MQ=255)                                                                                                         aCGGCAGGCTTCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTCATATCTACGCCCATGCGCGATACGGCg                    >  2:216724/1‑140 (MQ=255)                                                                                                                   tCGATCGCCACATGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTCATATCTACGCCCATGCGCGATACGGCAATTTTTTccc          >  2:836364/1‑140 (MQ=255)                                                                                                                           cacaTGCAGGCCTTTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTCATATCTACGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGCAg  >  2:55969/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                ACATCTTCCAGTTGATATTGTTCGATGAGGGTGCGCAGGCGTCTTTCCCACGGGCCAATGCCGAGGATGCGATAGCGAAATGCCACGCCCTGCTCTTTCAACTGACGGCAGGCTTCGATCGCCACATGCAGGCC‑TTTTTTCTCGGTTAAGCGTGCGACGGAAATAATCTCCAGCGGCGTTGCGGGCGCTTTCACGGGACGCGGGCTAAAGCGCGTCATATCTACGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGCAG  >  NC_000913/2116289‑2116549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑