Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,114,688	(C)5→6	coding (4425/4563 nt)	yghJ ←	putative lipoprotein YghJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,114,683	1	.	C	100.0%	151.0 / NA	43	A1477G (GCG→GGG)	yghJ	putative lipoprotein YghJ
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (22/21);  total (22/21)

TCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCG‑CCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAG  >  NC_000913/3114554‑3114806                                                                                                                                   |                                                                                                                            tCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCG‑ccccccggca                                                                                                                   <  2:924679/140‑6 (MQ=255)         cATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTaa                                                                                                            >  1:196672/1‑140 (MQ=255)          aTCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAg                                                                                                           >  2:1268978/1‑140 (MQ=255)                    cGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGcc                                                                                                 >  1:1050277/1‑140 (MQ=255)                    cGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGcc                                                                                                 >  2:296506/1‑140 (MQ=255)                    cGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGcc                                                                                                 >  1:593105/1‑140 (MQ=255)                      gTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCg                                                                                               >  1:409186/1‑140 (MQ=255)                      gTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCg                                                                                               >  2:220969/1‑140 (MQ=255)                          ccTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCAtt                                                                                                       >  2:693072/1‑128 (MQ=255)                          ccTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCAtt                                                                                                       <  1:693072/128‑1 (MQ=255)                           cTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTc                                                                                          >  1:717791/1‑140 (MQ=255)                            tGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTcc                                                                                         <  2:359164/140‑1 (MQ=255)                             gATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCa                                                                                        <  1:296506/140‑1 (MQ=255)                               ttAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTcc                                                                                         <  1:132282/137‑1 (MQ=255)                               ttAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTcc                                                                                         >  2:132282/1‑137 (MQ=255)                               ttAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCAtt                                                                                      <  2:16706/140‑1 (MQ=255)                               ttAATGGTTTCAGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCAtt                                                                                      >  1:1178380/1‑140 (MQ=255)                                     gTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTaa                                                                                >  1:1270486/1‑140 (MQ=255)                                        tCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGa                                                                             >  2:517378/1‑140 (MQ=255)                                        tCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGa                                                                             >  1:377320/1‑140 (MQ=255)                                        tCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGa                                                                             >  2:1030160/1‑140 (MQ=255)                                        tCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGa                                                                             >  2:361345/1‑140 (MQ=255)                                        tCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGa                                                                             >  2:354446/1‑140 (MQ=255)                                          cGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAAc                                                                           <  2:409186/140‑1 (MQ=255)                                                    tCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGAt                                                                 <  1:517378/140‑1 (MQ=255)                                                    tCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGAt                                                                 <  2:196672/140‑1 (MQ=255)                                                         tttCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTc                                                            >  1:814008/1‑140 (MQ=255)                                                           tCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTg                                                          >  2:608037/1‑140 (MQ=255)                                                                   tCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGAccc                                                  <  2:1270486/140‑1 (MQ=255)                                                                   tCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGAccc                                                  <  1:361345/140‑1 (MQ=255)                                                                      aGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCagg                                               <  2:377320/140‑1 (MQ=255)                                                                           cgcgAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGca                                          <  2:814008/140‑1 (MQ=255)                                                                                         aaGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCgt                            <  1:1030160/140‑1 (MQ=255)                                                                                         aaGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCgt                            <  1:354446/140‑1 (MQ=255)                                                                                            ccGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTc                         <  2:1178380/140‑1 (MQ=255)                                                                                            ccGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTc                         >  2:1105904/1‑140 (MQ=255)                                                                                              gACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCg                       <  2:717791/140‑1 (MQ=255)                                                                                               aCTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGc                      >  2:570462/1‑140 (MQ=255)                                                                                               aCTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGc                      <  1:485296/140‑1 (MQ=255)                                                                                               aCTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGc                      >  1:184213/1‑140 (MQ=255)                                                                                                    cTCACACCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGt                 <  1:570462/140‑1 (MQ=255)                                                                                                         acCGCCCTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCg            <  2:593105/140‑1 (MQ=255)                                                                                                               cTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCg                  >  2:197880/1‑128 (MQ=255)                                                                                                               cTCGAAGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCg                  <  1:197880/128‑1 (MQ=255)                                                                                                                   aaGCTCATCTCGCTCGCCCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAg  <  2:1050277/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                            TCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGAAGCTCATCTCGCTCG‑CCCCCGGCAGCTGGTAAGCATTCGCGCCCGGATTCCATTTCTTAAAGAACTCCGAAAGATCCGTCTGGGCGACCCAGGAGGCACACAGCATCAGCGTGTCCGCTGCGTTACCGTTGGATTCAG  >  NC_000913/3114554‑3114806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑