Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	48,498	G→A	G244S (GGC→AGC)	kefC →	K(+) : H(+) antiporter KefC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	48,498	0	G	A	100.0%	166.1 / NA	48	G244S (GGC→AGC)	kefC	K(+) : H(+) antiporter KefC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/22);  total (26/22)

TGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAT‑CGGTGTTGGCATGTCGATAGACTTTGGCACG  >  NC_000913/48361‑48632                                                                                                                                          |                                                                                                                                         tGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAgc                                                                                                                                        >  1:911316/1‑140 (MQ=255) tGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAgc                                                                                                                                        >  1:150507/1‑140 (MQ=255)     gcgcTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGt                                                                                                                                    >  1:28801/1‑140 (MQ=255)      cgcTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGtt                                                                                                                                   >  2:1121845/1‑140 (MQ=255)        cTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTc                                                                                                                                 >  1:1210814/1‑140 (MQ=255)           tGTCACGCGTCCGGCGCCGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTgg                                                                                                                              <  2:1210814/140‑1 (MQ=255)               aCGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTggcggg                                                                                                                          >  2:391371/1‑140 (MQ=255)                cgcgTCCGGCGCGGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTggcgggc                                                                                                                         >  2:1002267/1‑140 (MQ=255)                 gcgTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTggcgggcg                                                                                                                        <  1:391371/140‑1 (MQ=255)                   gTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑a                                                                                                                     >  1:221482/1‑140 (MQ=255)                     ccGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑Act                                                                                                                   >  1:945579/1‑140 (MQ=255)                       ggCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑Actgc                                                                                                                 >  2:553375/1‑140 (MQ=255)                          gcTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTgg                                                                                                              <  1:1121845/140‑1 (MQ=255)                                  ttGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCg                                                                                                      <  1:1002267/140‑1 (MQ=255)                                  ttGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCg                                                                                                      <  1:670200/140‑1 (MQ=255)                                        cccGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGc                                                                                                             >  1:962102/1‑127 (MQ=255)                                        cccGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGc                                                                                                             <  2:962102/127‑1 (MQ=255)                                        cccGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATAc                                                                                                >  1:27496/1‑140 (MQ=255)                                          cGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCg                                                                                              >  1:540875/1‑140 (MQ=255)                                            ctctGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTc                                                                                            <  1:183774/140‑1 (MQ=255)                                              ctGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCAt                                                                                          <  2:1364215/140‑1 (MQ=255)                                                 gCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTTGCAAGCTCCGGAAACCGTCATtcg                                                                                       >  2:183774/1‑140 (MQ=255)                                                 gCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATgcg                                                                                       <  1:553375/140‑1 (MQ=255)                                                  cTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATgcgc                                                                                      <  2:757993/140‑1 (MQ=255)                                                    tGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTg                                                                                    <  1:757755/140‑1 (MQ=255)                                                      cGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGG‑TGTGCTTGCCAGCTCCGGAAAACGCCATGCGCTGga                                                                                  >  2:606423/1‑140 (MQ=255)                                                       gggAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGgag                                                                                 >  2:851685/1‑140 (MQ=255)                                                                  aGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGGATACCGTCATGCGCTGGAGGGCGGTATTGa                                                                      >  2:926128/1‑140 (MQ=255)                                                                   gTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGaa                                                                     <  2:28801/140‑1 (MQ=255)                                                                                 ttCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGttt                                                       <  1:341195/140‑1 (MQ=255)                                                                                      cGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGG‑ACTGCTGGGCAGCTCGGGATACCGTCATGCGCTGGAGAGGGATATCGAACCATTTTAAAGGTTGCTGt                                                  >  2:341195/1‑140 (MQ=255)                                                                                        tgtTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGG‑ACTGGTGGCGAGCTCGGAATAACGCCAAGCGCTGGAGAGCGATATCGAAGCATTTTAAGGATTTCTGTTg                                                >  2:121361/1‑140 (MQ=255)                                                                                           ttGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTgggg                                             <  1:823669/140‑1 (MQ=255)                                                                                            tGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGc                                            <  1:851685/140‑1 (MQ=255)                                                                                                 ttGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGttt                                       <  2:150507/140‑1 (MQ=255)                                                                                                      ttGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAt                                  <  2:540875/140‑1 (MQ=255)                                                                                                         ctgctgGAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAT‑Cgg                              <  2:221482/140‑1 (MQ=255)                                                                                                              ggAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGGTTGCTGTTGGGGCTGTTTTTTAT‑TGGGGTTg                         >  1:645648/1‑140 (MQ=255)                                                                                                               gAAGAGGTCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGGTTGCTGTTGGGGCTGTTTTTCAT‑CGGGGTTgg                        >  1:885848/1‑140 (MQ=255)                                                                                                                      tCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGGCATGCGCTGGGGAGGGGTATCGCAACACTTTAAGGTTTGCTGTTGGGGCTGTTTTTCATCCGGGGTTggatttcg                  >  2:1303783/1‑134 (MQ=255)                                                                                                                      tCGGCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGG‑ACTGCTGGCAAGCTCGGGATACCGTCATGCGCTGGGGAGCGATATCGAACCCTTTAAAGGGTTGCTGTTGGGGGTGTTTTTCAT‑CGGGGTTGGCATGTCg                 >  2:410243/1‑140 (MQ=255)                                                                                                                        ggCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCTCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAT‑CGGTGTTGGCATGTCGAt               <  2:945579/140‑1 (MQ=255)                                                                                                                        ggCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAT‑CGGTGTTGGCATGTCGAt               <  1:606423/140‑1 (MQ=255)                                                                                                                         gCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGGGTGGTTTCTGGCCAGCTCGGAATAACGTCATGCGCTGGGGAGCGATATCGAACCATTTTAAGGTTTGCTGTTGGGGCTGGTTTTCAT‑CGGGGTTGGCATGTCGAt               >  2:464990/1‑140 (MQ=255)                                                                                                                         gCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAT‑CGGTGTTGGCATGTCGAt               <  2:34660/139‑1 (MQ=255)                                                                                                                         gCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAT‑CGGTGTTGGCATGTCGAt               >  1:34660/1‑139 (MQ=255)                                                                                                                         gCTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAT‑CGGTGTTGGCATGTCGATa              <  1:1303783/140‑1 (MQ=255)                                                                                                                          cTTGTCGATGGCGATGAGCGCGTTTCTGGCGGGCGG‑ACTGCTGGCAAGCTCGGGATACCGTCATGGGCTGGGGAGCGATATCGAACCATTTTAAGGTTTGCTGTTGGGGGTGTTTTTCAT‑CGGGGTTGGCATGTCGATAg             >  2:784811/1‑140 (MQ=255)                                                                                                                                   ggcgatgaGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAT‑CGGTGTTGGCATGTCGATAGACTTTGGCa    <  1:213976/140‑1 (MQ=255)                                                                                                                                     cgatgaGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAT‑CGGTGTTGGCATGTCGATAGACTTTGGCACg  <  1:629700/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TGGGGCGCTATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGTGCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCGATGGCGATGGGCGCGTTTCTGGCGGGCGT‑ACTGCTGGCAAGCTCGGAATACCGTCATGCGCTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCAT‑CGGTGTTGGCATGTCGATAGACTTTGGCACG  >  NC_000913/48361‑48632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑