Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	265,949	T→G	K47Q (AAG→CAG)	ykfB ←	CP4‑6 prophage; protein YkfB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	265,949	0	T	G	100.0%	281.1 / NA	79	K47Q (AAG→CAG)	ykfB	CP4‑6 prophage; protein YkfB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (44/35);  total (44/35)

GATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTG  >  NC_000913/265814‑266083                                                                                                                                        |                                                                                                                                       gATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCg                                                                                                                                    <  1:128608/140‑1 (MQ=255)    ccATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTg                                                                                                                                 >  2:455493/1‑140 (MQ=255)      aTCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGc                                                                                                                               >  2:26585/1‑140 (MQ=255)         aCTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTc                                                                                                                            >  1:681521/1‑140 (MQ=255)         aCTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTc                                                                                                                            >  1:331278/1‑140 (MQ=255)         aCTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTc                                                                                                                            >  1:150382/1‑140 (MQ=255)           tACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCgg                                                                                                                          <  1:455493/140‑1 (MQ=255)            aCGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGt                                                                                                                         >  1:556267/1‑140 (MQ=255)            aCGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGt                                                                                                                         >  1:818678/1‑140 (MQ=255)            aCGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGt                                                                                                                         <  2:1267594/140‑1 (MQ=255)             cGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTc                                                                                                                        >  1:55493/1‑140 (MQ=255)              ggTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCa                                                                                                                       >  1:1090774/1‑140 (MQ=255)              ggTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCa                                                                                                                       >  2:739264/1‑140 (MQ=255)              ggTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCa                                                                                                                       <  1:915685/140‑1 (MQ=255)                tGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACg                                                                                                                     <  1:1244750/140‑1 (MQ=255)                tGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACg                                                                                                                     <  2:818678/140‑1 (MQ=255)                tGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACg                                                                                                                     <  2:556267/140‑1 (MQ=255)                 gATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTc                                                                                                                        >  2:451668/1‑136 (MQ=255)                 gATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTc                                                                                                                        <  1:451668/136‑1 (MQ=255)                  aTAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACg                                                                                                                     <  2:1325289/138‑1 (MQ=255)                  aTAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACg                                                                                                                     >  1:1325289/1‑138 (MQ=255)                  aTAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCt                                                                                                                   <  1:739264/140‑1 (MQ=255)                  aTAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCt                                                                                                                   <  2:331278/140‑1 (MQ=255)                    agtagtGTCGTCATCGTGCGAACTGACCATCTT‑TCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTa                                                                                                                >  1:213855/1‑140 (MQ=255)                      tagtGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTAt                                                                                                               >  1:877272/1‑140 (MQ=255)                        gtgtCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTc                                                                                                             >  1:119679/1‑140 (MQ=255)                         tgtCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACg                                                                                                                     >  1:935795/1‑131 (MQ=255)                         tgtCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACg                                                                                                                     <  2:935795/131‑1 (MQ=255)                         tgtCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTcc                                                                                                            >  2:299146/1‑140 (MQ=255)                         tgtCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTcc                                                                                                            >  1:235801/1‑140 (MQ=255)                         tgtCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTcc                                                                                                            >  1:471456/1‑140 (MQ=255)                         tgtCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTcc                                                                                                            >  2:825832/1‑140 (MQ=255)                           tcgtcATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGt                                                                                                          <  1:825832/140‑1 (MQ=255)                            cgtcATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTa                                                                                                         >  1:1033266/1‑140 (MQ=255)                            cgtcATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTa                                                                                                         >  1:1109844/1‑140 (MQ=255)                            cgtcATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTa                                                                                                         <  2:1090774/140‑1 (MQ=255)                              tcATCGTGCGAACTGACCTTCTTGTCATCGCCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGCGTTCCCTGCGCCGTTCATCGTAAACGTCTGTCCGCTGGCCTCCGTCACCCTGTATTCCGCAtc                                                                                                       >  2:943836/1‑139 (MQ=255)                              tcATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTAtt                                                                                                       >  2:1166795/1‑140 (MQ=255)                                 tCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGcc                                                                                                    <  2:150382/140‑1 (MQ=255)                                 tCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGcc                                                                                                    <  1:380258/140‑1 (MQ=255)                                 tCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGcc                                                                                                    <  2:681521/140‑1 (MQ=255)                                 tCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGcc                                                                                                    <  1:1166795/140‑1 (MQ=255)                                   gTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCt                                                                                                  >  2:1332420/1‑140 (MQ=255)                                   gTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCt                                                                                                  <  1:299146/140‑1 (MQ=255)                                    tGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTg                                                                                                 >  1:163543/1‑140 (MQ=255)                                     gCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCt                                                                                                  <  2:177219/138‑1 (MQ=255)                                     gCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCt                                                                                                  >  1:177219/1‑138 (MQ=255)                                     gCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGa                                                                                                >  2:1079341/1‑140 (MQ=255)                                           tGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTaa                                                                                          >  1:915847/1‑140 (MQ=255)                                                 tCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCctgc                                                                                    <  2:55493/140‑1 (MQ=255)                                                 tCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCctgc                                                                                    >  2:279376/1‑140 (MQ=255)                                                  cTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCctgct                                                                                   >  1:49627/1‑140 (MQ=255)                                                   ttGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCct                                                                                      >  1:158883/1‑136 (MQ=255)                                                   ttGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCct                                                                                      <  2:158883/136‑1 (MQ=255)                                                    tGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCtgctgc                                                                                 <  2:306877/140‑1 (MQ=255)                                                     gTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGcc                                                                                <  2:877272/140‑1 (MQ=255)                                                       cATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAg                                                                              >  2:1274113/1‑140 (MQ=255)                                                            gCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAAccc                                                                         >  2:57300/1‑140 (MQ=255)                                                                gggTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCggg                                                                     <  1:1079341/140‑1 (MQ=255)                                                                       aaagaCTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCag                                                              <  1:57300/140‑1 (MQ=255)                                                                           aCTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAAccc                                                                         >  1:106641/1‑125 (MQ=255)                                                                           aCTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAAccc                                                                         <  2:106641/125‑1 (MQ=255)                                                                                 tGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTaaa                                                    >  2:704719/1‑140 (MQ=255)                                                                                     gATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTc                                                >  2:290036/1‑140 (MQ=255)                                                                                     gATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTc                                                <  1:704719/140‑1 (MQ=255)                                                                                        aGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTcgcg                                             <  1:319054/140‑1 (MQ=255)                                                                                             ccATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAg                                        >  2:433843/1‑140 (MQ=255)                                                                                                   tGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGACCCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACAcc                                  <  1:926013/140‑1 (MQ=255)                                                                                                     gTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCg                                >  1:1347783/1‑140 (MQ=255)                                                                                                     gTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCg                                <  1:26585/140‑1 (MQ=255)                                                                                                     gTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCg                                >  1:690533/1‑140 (MQ=255)                                                                                                      tCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGc                               >  1:93515/1‑140 (MQ=255)                                                                                                             tCCCTGTGCAGTTGATGGTAAACGTCTGCCCGCTGGCTCCGGCCACGCTGTATCCCGTATTGCCCTGACCGTACCCCTGCCGCCAGACCCCCGGGATAGCAGAGGCATTAAAGCCCGCGAGCAGTACACCCGCCAGCATa                        <  2:163543/140‑1 (MQ=25)                                                                                                                   tgCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGCCCGTAACCCTGCTGCCAGACCCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACCCCCGCCAGCATAAACCGa                  <  2:471456/140‑1 (MQ=255)                                                                                                                   tgCAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGGGGCATTAAAGCTCGCGAGCAGTACCCCCGCCAGCATAAACCGa                  <  2:235801/140‑1 (MQ=255)                                                                                                                     cAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGACCCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCa                          <  1:264660/130‑1 (MQ=255)                                                                                                                     cAGTTGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCa                          >  2:264660/1‑130 (MQ=255)                                                                                                                        ttGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGACCCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAg             <  1:206124/140‑1 (MQ=255)                                                                                                                        ttGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAg             <  1:433843/140‑1 (MQ=255)                                                                                                                        ttGATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAg             >  1:163114/1‑140 (MQ=255)                                                                                                                          gATGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTg           >  2:1039786/1‑140 (MQ=255)                                                                                                                           aTGGTAACCGTCTGTCCGCTGGTTTCGTTCTCGCTGTATCCCGTATTGCCCGGCCCGTACCCCTCCTCCCAGACCCCCGGGATAGCAGGGGCATTAAAGCGCGCGAGCAGTCCACCCGCCAGCATAAACCGACTTAGTGa          <  2:1033266/140‑1 (MQ=255)                                                                                                                           aTGGTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGACCCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTGa          <  1:1274113/140‑1 (MQ=255)                                                                                                                             ggTAAACGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTGaaa        >  1:336316/1‑140 (MQ=255)                                                                                                                                aaaCGTCTGTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTCCCAGACCCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTGAAAGTa     <  2:119679/140‑1 (MQ=255)                                                                                                                                   cGTCTGTCCGCTGGCTTCGGTCCCGCTGTATTCCGTATTGCCCTGCCCGTAACCCTGCTGCCAGAACCCGGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTg  <  2:213855/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCCGGGTTCCCTGTGCAGTTGATGGTAAACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGGATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTG  >  NC_000913/265814‑266083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑