Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,338,527	T→C	H241R (CAC→CGC)	mlaE ←	intermembrane phospholipid transport system, integral membrane subunit MlaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,338,527	0	T	C	100.0%	311.9 / NA	86	H241R (CAC→CGC)	mlaE	intermembrane phospholipid transport system, integral membrane subunit MlaE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/41);  total (45/41)

AACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCAT  >  NC_000913/3338390‑3338660                                                                                                                                          |                                                                                                                                      aacaaacaGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCgg                                                                                                                                     >  1:25621/1‑140 (MQ=255)   caaacaGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGAc                                                                                                                                   <  2:9391/140‑1 (MQ=255)   caaacaGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGAc                                                                                                                                   <  1:985809/140‑1 (MQ=255)            ccgccAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCg                                                                                                                          >  2:196190/1‑140 (MQ=255)              gccAGCAGTGCTGCTAATAAAAAGATACCCA‑CCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGt                                                                                                                       >  2:443992/1‑140 (MQ=255)               ccAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGt                                                                                                                       <  1:101921/140‑1 (MQ=255)                        ctgctAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCg                                                                                                              >  2:767213/1‑140 (MQ=255)                                aaaaaGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATcc                                                                                                      >  2:937517/1‑140 (MQ=255)                                  aaaGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCg                                                                                                    >  1:894506/1‑140 (MQ=255)                                   aaGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCgg                                                                                                   >  1:1155944/1‑140 (MQ=255)                                   aaGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCgg                                                                                                   <  1:185928/140‑1 (MQ=255)                                     gATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCa                                                                                                 >  1:987720/1‑140 (MQ=255)                                      aTACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAg                                                                                                >  2:770655/1‑140 (MQ=255)                                       tACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGa                                                                                               >  1:945076/1‑140 (MQ=255)                                        acccacccAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGAc                                                                                              >  2:179812/1‑140 (MQ=255)                                         cccacccaAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACg                                                                                             >  2:847942/1‑140 (MQ=255)                                          cccaccaAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGt                                                                                            <  1:443992/136‑1 (MQ=255)                                          ccacccaAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGt                                                                                            >  1:699552/1‑140 (MQ=255)                                             cccaAATTTCATTTTTTTTTGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCgg                                                                                         >  1:762678/1‑140 (MQ=255)                                             cccaAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCgg                                                                                         >  2:1061207/1‑140 (MQ=255)                                             cccaAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCgg                                                                                         >  1:1247959/1‑140 (MQ=255)                                              ccaAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCggg                                                                                        <  2:514615/140‑1 (MQ=255)                                               caAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGa                                                                                       <  2:699552/140‑1 (MQ=255)                                               caAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGa                                                                                       <  1:847942/140‑1 (MQ=255)                                                 aaTTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATg                                                                                     <  2:549401/140‑1 (MQ=255)                                                 aaTTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATg                                                                                     <  2:764723/140‑1 (MQ=255)                                                        ttttttttCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGcgtcgt                                                                              >  1:398924/1‑140 (MQ=255)                                                        ttttttttCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGcgtcgt                                                                              >  2:409368/1‑140 (MQ=255)                                                                  tttGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTaaa                                                                    <  2:371303/140‑1 (MQ=255)                                                                      cATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAaacaac                                                                >  2:587210/1‑140 (MQ=255)                                                                         gAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGaa                                                             <  2:945076/140‑1 (MQ=255)                                                                               aaTTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTaaa                                                                    <  2:181835/127‑1 (MQ=255)                                                                               aaTTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTaaa                                                                    >  1:181835/1‑127 (MQ=255)                                                                                aTTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACg                                                      >  2:231410/1‑140 (MQ=255)                                                                                 ttCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGt                                                     >  2:413868/1‑140 (MQ=255)                                                                                  tCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTc                                                    <  1:731432/140‑1 (MQ=255)                                                                                   cccAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCa                                                   <  2:894506/140‑1 (MQ=255)                                                                                      aaaCATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCg                                                >  2:181991/1‑140 (MQ=255)                                                                                       aaCATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGt                                               >  2:418754/1‑140 (MQ=255)                                                                                         cATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGa                                             >  2:622392/1‑140 (MQ=255)                                                                                           tCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGATGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATg                                           >  1:1053753/1‑140 (MQ=255)                                                                                            cAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATgg                                          >  2:1079436/1‑140 (MQ=255)                                                                                                     cAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAAcacca                                 <  2:82697/140‑1 (MQ=255)                                                                                                     cAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAAcacca                                 <  2:398924/140‑1 (MQ=255)                                                                                                     cAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAAcacca                                 <  2:987720/140‑1 (MQ=255)                                                                                                     cAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAAcacca                                 <  1:937517/140‑1 (MQ=255)                                                                                                      aGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAAcaccac                                >  2:404259/1‑140 (MQ=255)                                                                                                      aGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAAcaccac                                >  1:443229/1‑140 (MQ=255)                                                                                                       gCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACg                               >  2:1356819/1‑140 (MQ=255)                                                                                                        cacaAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGc                              >  2:488178/1‑140 (MQ=255)                                                                                                        cacaAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGc                              >  2:254507/1‑140 (MQ=255)                                                                                                         acaAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGct                             <  1:792641/140‑1 (MQ=255)                                                                                                         acaAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGct                             <  1:413868/140‑1 (MQ=255)                                                                                                         acaAAATCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGct                             <  2:1155944/140‑1 (MQ=255)                                                                                                            aaaTCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCtt                          <  1:587210/140‑1 (MQ=255)                                                                                                             aaTCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGct                             >  2:150966/1‑136 (MQ=255)                                                                                                             aaTCCAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGct                             <  1:150966/136‑1 (MQ=255)                                                                                                                 cAGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCa                     <  2:226721/140‑1 (MQ=255)                                                                                                                  aGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAg                    >  1:277065/1‑140 (MQ=255)                                                                                                                  aGCCCCAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAg                    >  2:848030/1‑140 (MQ=255)                                                                                                                      ccAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAg                <  2:762678/140‑1 (MQ=255)                                                                                                                      ccAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAg                <  1:622392/140‑1 (MQ=255)                                                                                                                      ccAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAg                <  1:196190/140‑1 (MQ=255)                                                                                                                      ccAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAg                <  1:848030/140‑1 (MQ=255)                                                                                                                      ccAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAg                <  2:25621/140‑1 (MQ=255)                                                                                                                      ccAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAg                <  1:941546/140‑1 (MQ=255)                                                                                                                       cAGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGt               >  1:47522/1‑140 (MQ=255)                                                                                                                        aGAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGtt              >  2:190216/1‑140 (MQ=255)                                                                                                                         gAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTg             >  2:894258/1‑140 (MQ=255)                                                                                                                         gAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTg             >  1:1233356/1‑140 (MQ=255)                                                                                                                         gAACAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTg             <  1:1061207/140‑1 (MQ=255)                                                                                                                           aCAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGAc           <  1:894258/140‑1 (MQ=255)                                                                                                                           aCAGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGAc           <  1:1153279/140‑1 (MQ=255)                                                                                                                             aGCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCa         <  1:254507/140‑1 (MQ=255)                                                                                                                              gCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAg        <  1:1356819/140‑1 (MQ=255)                                                                                                                              gCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAg        <  1:1079436/140‑1 (MQ=255)                                                                                                                              gCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAg        <  2:443229/140‑1 (MQ=255)                                                                                                                              gCCAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAg        <  1:231410/140‑1 (MQ=255)                                                                                                                               ccAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGa       >  1:499351/1‑140 (MQ=255)                                                                                                                               ccAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGa       >  2:377077/1‑140 (MQ=255)                                                                                                                               ccAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGa       >  1:1207725/1‑140 (MQ=255)                                                                                                                               ccAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGa       >  2:1329995/1‑140 (MQ=255)                                                                                                                               ccAGAGACGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGa       >  2:1365084/1‑140 (MQ=255)                                                                                                                                   agaCGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCa   <  2:277065/140‑1 (MQ=255)                                                                                                                                   agaCGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCa   <  2:1233356/140‑1 (MQ=255)                                                                                                                                    gaCGAGCGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCAt  >  1:425776/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      AACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCAT  >  NC_000913/3338390‑3338660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑