Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,399,266	A→G	V238A (GTC→GCC)	mreC ←	cell shape determining protein MreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,399,266	0	A	G	100.0%	229.7 / NA	65	V238A (GTC→GCC)	mreC	cell shape determining protein MreC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/30);  total (35/30)

CGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAAT  >  NC_000913/3399133‑3399402                                                                                                                                      |                                                                                                                                         cGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACcg                                                                                                                                    <  1:877193/140‑1 (MQ=255)   gCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACcgcg                                                                                                                                  >  2:829941/1‑140 (MQ=255)   gCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACcgcg                                                                                                                                  <  2:346310/140‑1 (MQ=255)    cGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGa                                                                                                                                 <  1:574149/140‑1 (MQ=255)    cGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGa                                                                                                                                 <  2:406729/140‑1 (MQ=255)      tCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGAcc                                                                                                                               <  1:79803/140‑1 (MQ=255)        aTCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCgg                                                                                                                             >  2:690351/1‑140 (MQ=255)         tCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGa                                                                                                                            <  2:333892/140‑1 (MQ=255)             gTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGc                                                                                                                        <  1:690351/140‑1 (MQ=255)             gTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGc                                                                                                                        <  1:538379/140‑1 (MQ=255)                 gcgcCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTc                                                                                                                    >  1:322868/1‑140 (MQ=255)                 gcgcCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTc                                                                                                                    <  1:829941/140‑1 (MQ=255)                   gcCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCg                                                                                                                  >  2:995650/1‑140 (MQ=255)                      gTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGATGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGa                                                                                                               <  1:145702/140‑1 (MQ=255)                       ttACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATa                                                                                                                          <  1:356099/128‑1 (MQ=255)                       ttACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATa                                                                                                                          >  2:356099/1‑128 (MQ=255)                       ttACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGaa                                                                                                              >  1:177642/1‑140 (MQ=255)                        tACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGaaa                                                                                                             >  2:707870/1‑140 (MQ=255)                              cTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCg                                                                                                       <  1:36597/140‑1 (MQ=255)                                gCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGcc                                                                                                     <  1:995650/140‑1 (MQ=255)                                    cccACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGa                                                                                                 >  2:547399/1‑140 (MQ=255)                                      cacaGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGAcc                                                                                               >  1:1080842/1‑140 (MQ=255)                                       acaGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCg                                                                                              <  2:322868/140‑1 (MQ=255)                                           cagcagcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAg                                                                                          >  1:285663/1‑140 (MQ=255)                                           cagcagcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAg                                                                                          >  2:16610/1‑140 (MQ=255)                                              cagcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCa                                                                                       >  1:689910/1‑140 (MQ=255)                                              cagcagATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCa                                                                                       >  2:296826/1‑140 (MQ=255)                                                        cGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATc                                                                             <  2:1080842/140‑1 (MQ=255)                                                           aaaCGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCAcc                                                                          >  1:585038/1‑140 (MQ=255)                                                             aCGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCaa                                                                        >  1:989358/1‑140 (MQ=255)                                                              cGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAg                                                                                   <  2:921160/128‑1 (MQ=255)                                                              cGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAg                                                                                   >  1:921160/1‑128 (MQ=255)                                                                  gCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGa                                                                   >  1:1252982/1‑140 (MQ=255)                                                                   cAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGaa                                                                  >  1:258948/1‑140 (MQ=255)                                                                   cAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGaa                                                                  <  1:144767/140‑1 (MQ=255)                                                                     gCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAata                                                                >  1:1175864/1‑140 (MQ=255)                                                                         tGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAGGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCg                                                            >  2:1232304/1‑140 (MQ=255)                                                                           cAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTa                                                          <  2:830774/140‑1 (MQ=255)                                                                                ggACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGc                                                     >  1:759905/1‑140 (MQ=255)                                                                                   cgcgCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCgg                                                  >  2:813991/1‑140 (MQ=255)                                                                                     cgcCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCa                                                <  1:990048/140‑1 (MQ=255)                                                                                     cgcCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCa                                                <  2:606741/140‑1 (MQ=255)                                                                                           aaTCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCt                                          <  2:619810/140‑1 (MQ=255)                                                                                             tCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCa                                        <  1:139641/140‑1 (MQ=255)                                                                                             tCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCa                                        <  1:547399/140‑1 (MQ=255)                                                                                                caGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGc                                     >  1:862302/1‑140 (MQ=255)                                                                                                      aaGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCaaa                               >  2:63168/1‑140 (MQ=255)                                                                                                       aGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCt                                          <  2:1330236/128‑1 (MQ=255)                                                                                                       aGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCt                                          >  1:1330236/1‑128 (MQ=255)                                                                                                       aGCGCGCTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAat                              <  2:658838/140‑1 (MQ=255)                                                                                                         cgcgcTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAatca                            >  1:377317/1‑140 (MQ=255)                                                                                                          gcgcTGGGTATCGAGTTTTACGGAGGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAatcat                           <  1:1232304/140‑1 (MQ=255)                                                                                                           cgcTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAatcatc                          >  1:565502/1‑140 (MQ=255)                                                                                                             cTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCg                        >  2:582065/1‑140 (MQ=255)                                                                                                             cTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCg                        >  2:518786/1‑140 (MQ=255)                                                                                                             cTGGGTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCg                        >  2:461611/1‑140 (MQ=255)                                                                                                               gggTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTa                      >  2:806521/1‑140 (MQ=255)                                                                                                               gggTATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTa                      >  2:1151122/1‑140 (MQ=255)                                                                                                                  tATCGAGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACaa                   <  1:461611/140‑1 (MQ=255)                                                                                                                       aGTTTTACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGtt              <  2:1187754/140‑1 (MQ=255)                                                                                                                          tttACGGAAGAGGCAACCGCGTCCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAcc           >  2:965947/1‑140 (MQ=255)                                                                                                                          tttACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAcc           <  1:518786/140‑1 (MQ=255)                                                                                                                          tttACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAcc           <  2:285663/140‑1 (MQ=255)                                                                                                                            tACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCgg         >  2:927872/1‑140 (MQ=255)                                                                                                                            tACGGAAGAGGCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCgg         >  2:997023/1‑140 (MQ=255)                                                                                                                                   gaggCAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAAt  <  2:862302/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAAT  >  NC_000913/3399133‑3399402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑