Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,462,060	T→C	S29S (AGT→AGC)	gspI →	Type II secretion system protein GspI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,462,060	0	T	C	100.0%	251.7 / NA	70	S29S (AGT→AGC)	gspI	Type II secretion system protein GspI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/36);  total (34/36)

GTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGTATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAAT  >  NC_000913/3461925‑3462195                                                                                                                                        |                                                                                                                                        gTTCAGGCTCATGGCCCCTGGATCAAACGCTCGACAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGc                                                                                                                                     <  1:162664/140‑1 (MQ=255)  ttCAGGCTCATGACCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCa                                                                                                                                    >  1:316041/1‑140 (MQ=255)       gCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGc                                                                                                                               >  2:902554/1‑140 (MQ=255)       gCTCATGGCCCCTGGATCAAACGCTCACCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCa                                                                                                                                    >  2:302011/1‑135 (MQ=255)       gCTCATGGCCCCTGGATCAAACGCTCACCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCa                                                                                                                                    <  1:302011/135‑1 (MQ=255)            tGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGa                                                                                                                          >  2:35769/1‑140 (MQ=255)                 ccTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGc                                                                                                                     >  2:1046969/1‑140 (MQ=255)                   tGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCa                                                                                                                   >  2:451824/1‑140 (MQ=255)                        cAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAg                                                                                                              <  2:1055820/140‑1 (MQ=255)                        cAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAg                                                                                                              <  2:760567/140‑1 (MQ=255)                         aaaCGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGc                                                                                                             <  1:1324929/140‑1 (MQ=255)                          aaCGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCg                                                                                                            <  1:1046969/140‑1 (MQ=255)                           aCGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGa                                                                                                           <  2:912366/140‑1 (MQ=255)                           aCGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGa                                                                                                           <  1:572689/140‑1 (MQ=255)                             gCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAAt                                                                                                         <  1:742890/140‑1 (MQ=255)                              cTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATg                                                                                                        <  2:812615/140‑1 (MQ=255)                               tCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGc                                                                                                       >  2:829585/1‑140 (MQ=255)                               tCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGc                                                                                                       >  1:467866/1‑140 (MQ=255)                                      agaTACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTCAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACga                                                                                                <  2:1270125/140‑1 (MQ=255)                                        aTACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACgaga                                                                                              <  1:451824/140‑1 (MQ=255)                                             cGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTg                                                                                         <  1:835440/140‑1 (MQ=255)                                               cccATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGc                                                                                       >  1:1158884/1‑140 (MQ=255)                                                  aTGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCAtt                                                                                    <  2:316041/140‑1 (MQ=255)                                                    gAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATtgt                                                                                  >  1:384135/1‑140 (MQ=255)                                                        aaacaaTCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGAt                                                                              <  2:1306601/140‑1 (MQ=255)                                                        aaacaaTCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGAt                                                                              <  2:1077542/140‑1 (MQ=255)                                                        aaacaaTCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGAt                                                                              <  2:384135/140‑1 (MQ=255)                                                             aTCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCgg                                                                         <  2:31644/140‑1 (MQ=255)                                                              tCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCggt                                                                        >  2:459761/1‑139 (MQ=255)                                                              tCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTCTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGa                                                                        >  1:795639/1‑140 (MQ=255)                                                               cAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGAt                                                                       >  1:306635/1‑140 (MQ=255)                                                                 gggATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGAt                                                                              <  2:347228/131‑1 (MQ=255)                                                                 gggATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGAt                                                                              >  1:347228/1‑131 (MQ=255)                                                                        cacTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCtt                                                              >  2:440267/1‑140 (MQ=255)                                                                        cacTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCtt                                                              >  2:36514/1‑140 (MQ=255)                                                                            gCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGt                                                          >  2:533450/1‑140 (MQ=255)                                                                            gCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGt                                                          >  2:538245/1‑140 (MQ=255)                                                                             cTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTc                                                         >  1:870730/1‑140 (MQ=255)                                                                             cTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTc                                                         >  1:577319/1‑140 (MQ=255)                                                                              ttGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTca                                                        >  2:305868/1‑140 (MQ=255)                                                                                 aaGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACaa                                                     >  1:593569/1‑140 (MQ=255)                                                                                     cTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGatt                                                 >  1:790067/1‑140 (MQ=255)                                                                                       tACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGattca                                               >  1:351831/1‑140 (MQ=255)                                                                                         cTGGCGATGAGTATTCTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGattcatt                                             <  2:795639/140‑1 (MQ=255)                                                                                           ggCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCg                                           <  1:440267/140‑1 (MQ=255)                                                                                           ggCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCg                                           >  2:487309/1‑140 (MQ=255)                                                                                           ggCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCg                                           >  2:526655/1‑140 (MQ=255)                                                                                           ggCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCg                                           <  1:231878/140‑1 (MQ=255)                                                                                           ggCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCg                                           <  1:36514/140‑1 (MQ=255)                                                                                            gCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCgg                                          >  2:760711/1‑140 (MQ=255)                                                                                             cGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGc                                         >  2:440231/1‑140 (MQ=255)                                                                                              gATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGt                                                          <  2:393470/122‑1 (MQ=255)                                                                                              gATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGt                                                          >  1:393470/1‑122 (MQ=255)                                                                                              gATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCg                                        >  1:354581/1‑140 (MQ=255)                                                                                               aTGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGa                                       >  2:433384/1‑140 (MQ=255)                                                                                                 gAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGTTAACCAGCTTCAGTCACAAGATTCATTCGGCGAgg                                     <  1:459761/140‑1 (MQ=255)                                                                                                    tATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGaaa                                  >  1:20475/1‑140 (MQ=255)                                                                                                     aTTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGaaaa                                 <  2:593569/140‑1 (MQ=255)                                                                                                            ccGCTGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTcc                          <  1:682931/140‑1 (MQ=255)                                                                                                                tGTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGtt                      <  1:538245/140‑1 (MQ=255)                                                                                                                 gTTGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTc                     <  2:467866/140‑1 (MQ=255)                                                                                                                  ttGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCt                    >  2:45287/1‑140 (MQ=255)                                                                                                                  ttGCTCTGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCt                    >  1:201283/1‑140 (MQ=255)                                                                                                                     ctctGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCTGGc                 <  1:902554/140‑1 (MQ=255)                                                                                                                      tctGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGTCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCTGCGAGGAAAACCCGTCCAGATCTGGCa                <  2:117795/140‑1 (MQ=255)                                                                                                                      tctGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCTGGCa                <  1:526655/140‑1 (MQ=255)                                                                                                                      tctGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCTGGCa                <  1:487309/140‑1 (MQ=255)                                                                                                                       ctGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCTGGCaa               <  1:440231/140‑1 (MQ=255)                                                                                                                       ctGACTTTAATGAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCTGGCaa               <  1:433384/140‑1 (MQ=255)                                                                                                                                  gAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTtaataa    <  1:305868/140‑1 (MQ=255)                                                                                                                                  gAGCAGCATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTtaataa    <  1:533450/140‑1 (MQ=255)                                                                                                                                    gcagcaTGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAAt  >  2:685739/1‑140 (MQ=255)                                                                                                                                    gcagcaTGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAAt  >  1:1276314/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATGACACTGCTTGAAGTCTTACTGGCGATGAGTATTTTCACCGCTGTTGCTCTGACTTTAATGAGCAGTATGCAAGGGCAACGAAATGCCATCGAGCGAATGCGTAACGAGACGCTGGCATTGTGGATAGCGGATAACCAGCTTCAGTCACAAGATTCATTCGGCGAGGAAAACACGTCCAGTTCTGGCAAAGAGTTAATAAAT  >  NC_000913/3461925‑3462195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑