Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	483,569	C→T	A279T (GCA→ACA)	acrB ←	multidrug efflux pump RND permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	483,569	0	C	T	100.0%	182.2 / NA	53	A279T (GCA→ACA)	acrB	multidrug efflux pump RND permease AcrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/31);  total (22/31)

AACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGC  >  NC_000913/483432‑483698                                                                                                                                          |                                                                                                                                  aaCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTga                                                                                                                                 >  2:577345/1‑140 (MQ=255)  aCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTgat                                                                                                                                >  1:980837/1‑140 (MQ=255)   cAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTgatg                                                                                                                               <  2:980837/140‑1 (MQ=255)             aCCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGt                                                                                                                     <  1:577345/140‑1 (MQ=255)                cGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCAGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTtct                                                                                                                  >  1:759652/1‑140 (MQ=255)                   cGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTcac                                                                                                               >  2:16768/1‑140 (MQ=255)                         gaaCGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCa                                                                                                         <  1:1094352/140‑1 (MQ=255)                            cGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCt                                                                                                      <  1:783167/140‑1 (MQ=255)                             ggTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTc                                                                                                     <  2:578639/140‑1 (MQ=255)                               ttCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCaa                                                                                                   >  2:161429/1‑140 (MQ=255)                                tCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAAt                                                                                                  >  1:372401/1‑140 (MQ=255)                                 ccATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATc                                                                                                 >  2:628221/1‑140 (MQ=255)                                      ttCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTcgc                                                                                            <  2:1234930/140‑1 (MQ=255)                                       tCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTcgcg                                                                                           <  2:143273/140‑1 (MQ=255)                                         gCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGTTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTcgcg                                                                                           <  2:286565/138‑1 (MQ=255)                                         gCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTcgcg                                                                                           >  1:286565/1‑138 (MQ=255)                                         gCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGAc                                                                                         >  2:59860/1‑140 (MQ=255)                                          ccAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACg                                                                                        <  1:161429/140‑1 (MQ=255)                                          ccAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACg                                                                                        <  1:862657/140‑1 (MQ=255)                                              ttCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCAc                                                                                    >  2:1040831/1‑140 (MQ=255)                                                  gCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcag                                                                                <  1:805771/140‑1 (MQ=255)                                                    aCGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcagca                                                                              <  1:59860/140‑1 (MQ=255)                                                     cGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcagcag                                                                             <  2:847383/140‑1 (MQ=255)                                                          tGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCAcgc                                                                        <  1:628221/140‑1 (MQ=255)                                                                  cGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCa                                                                >  2:1123395/1‑140 (MQ=255)                                                                            cgcgTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCgg                                                                      <  1:1240388/124‑1 (MQ=255)                                                                            cgcgTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCgg                                                                      >  2:1240388/1‑124 (MQ=255)                                                                            cgcgTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCa                                                      >  2:1239504/1‑140 (MQ=255)                                                                            cgcgTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCa                                                      >  2:1254309/1‑140 (MQ=255)                                                                              cgTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTTAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGaa                                                                   >  2:955849/1‑125 (MQ=255)                                                                              cgTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTTAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGaa                                                                   <  1:955849/125‑1 (MQ=255)                                                                                   gCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTcag                                               <  1:1239504/140‑1 (MQ=255)                                                                                         gTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGAt                                         >  2:1187393/1‑140 (MQ=255)                                                                                             ccAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTg                                     <  2:1208829/140‑1 (MQ=255)                                                                                             ccAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTg                                     <  1:1254309/140‑1 (MQ=255)                                                                                                gCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCg                                  <  2:126061/140‑1 (MQ=255)                                                                                                  ttGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa                                <  2:815475/140‑1 (MQ=255)                                                                                                  ttGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa                                <  2:217664/140‑1 (MQ=255)                                                                                                  ttGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGaa                                <  2:51002/140‑1 (MQ=255)                                                                                                   tGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCACCCTGATTCACTTTCAGCAGGATTTTGCCGAAc                               >  1:112999/1‑140 (MQ=255)                                                                                                     aTCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAActc                             <  1:1123395/140‑1 (MQ=255)                                                                                                            gACCGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTa                      >  1:1008242/1‑140 (MQ=255)                                                                                                              ccGGAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTaga                    >  1:156000/1‑140 (MQ=255)                                                                                                                 gAAGCCGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGt                 >  1:1013442/1‑140 (MQ=255)                                                                                                                     ccGGTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGa             <  1:1040831/140‑1 (MQ=255)                                                                                                                       ggTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAActc                             <  1:1350539/122‑1 (MQ=255)                                                                                                                       ggTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAActc                             >  2:686695/1‑122 (MQ=255)                                                                                                                       ggTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAActc                             >  2:1350539/1‑122 (MQ=255)                                                                                                                       ggTTGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAActc                             <  1:686695/122‑1 (MQ=255)                                                                                                                          tGGCCGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGt        <  2:372401/140‑1 (MQ=255)                                                                                                                             ccGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTg     <  1:1187393/140‑1 (MQ=255)                                                                                                                             ccGTTAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTg     <  1:16768/140‑1 (MQ=255)                                                                                                                                ttAAACTCTGTGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCACCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGc  <  2:112999/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  AACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGC  >  NC_000913/483432‑483698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑