BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,880,097	G→A	S8F (TCC→TTC)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,880,097	0	G	A	99.0%	333.6 / ‑4.9	97	S8F (TCC→TTC)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (40/55); total (41/56)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGC  >  NC_000913/3879961‑3880233

cgcgAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgaagg                                                                                                                                       <  1:1246504/140‑1 (MQ=255)

     gcgcTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCa                                                                                                                                  >  1:231317/1‑140 (MQ=255)

         ttCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAg                                                                                                                              >  2:94905/1‑140 (MQ=255)

           cgtcgATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGaa                                                                                                                            >  2:365119/1‑140 (MQ=255)

              cgATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTc                                                                                                                         <  1:98427/140‑1 (MQ=255)

              cgATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGCCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTc                                                                                                                         <  1:193623/140‑1 (MQ=255)

                  aGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGCAGGAGTCATAAGAATTCGACa                                                                                                                     <  2:963549/140‑1 (MQ=255)

                     gTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCa                                                                                                                  <  2:761978/140‑1 (MQ=255)

                          cTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGtt                                                                                                             >  1:659732/1‑140 (MQ=255)

                           tACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGttt                                                                                                            >  2:1028338/1‑140 (MQ=255)

                             ccaccTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTtct                                                                                                          <  2:231317/140‑1 (MQ=255)

                              caccTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTtctc                                                                                                         >  1:858686/1‑140 (MQ=255)

                               cccTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                                                                                                        <  2:373997/139‑1 (MQ=255)

                               accTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                                                                                                        <  2:858686/140‑1 (MQ=255)

                                ccTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGc                                                                                                       <  1:740304/140‑1 (MQ=255)

                                ccTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGc                                                                                                       <  1:365119/140‑1 (MQ=255)

                                 cTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCt                                                                                                      <  2:1210441/140‑1 (MQ=255)

                                 cTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCt                                                                                                      <  2:659732/140‑1 (MQ=255)

                                 cTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCt                                                                                                      <  2:867524/140‑1 (MQ=255)

                                 cTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCt                                                                                                      >  2:854365/1‑140 (MQ=255)

                                   cGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCa                                                                                                    >  2:1322503/1‑140 (MQ=255)

                                    gAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCAt                                                                                                   <  2:1048242/140‑1 (MQ=255)

                                     aaTACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCAtt                                                                                                  <  2:835888/140‑1 (MQ=255)

                                     aaTACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCAtt                                                                                                  <  1:614552/140‑1 (MQ=255)

                                     aaTACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCAtt                                                                                                  <  2:200461/140‑1 (MQ=255)

                                           aTGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                                                                                                        <  2:109534/128‑1 (MQ=255)

                                           aTGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                                                                                                        >  1:109534/1‑128 (MQ=255)

                                           aTGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACt                                                                                            <  1:1158763/140‑1 (MQ=255)

                                            tgtgGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGttt                                                                                                            >  1:613633/1‑123 (MQ=255)

                                            tgtgGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGttt                                                                                                            <  2:613633/123‑1 (MQ=255)

                                            tgtgGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACtt                                                                                           >  2:592/1‑140 (MQ=255)

                                              tggtgCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTgg                                                                                         >  1:1307688/1‑140 (MQ=255)

                                              tggtgCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTgg                                                                                         >  1:340492/1‑140 (MQ=255)

                                               ggtgCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTggg                                                                                        <  2:371727/140‑1 (MQ=255)

                                                gtgCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATATTTGGGt                                                                                       <  2:1129639/140‑1 (MQ=255)

                                                 tgCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTACACTTGGGtt                                                                                      >  2:1079512/1‑140 (MQ=255)

                                                 tgCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGtt                                                                                      <  1:706778/140‑1 (MQ=255)

                                                 tgCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGtt                                                                                      >  1:234654/1‑140 (MQ=255)

                                                 tgCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGtt                                                                                      <  2:340492/140‑1 (MQ=255)

                                                  gCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTTGCTCATTTATACTTGGGt                                                                                       <  1:630342/138‑1 (MQ=255)

                                                  gCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTTGCTCATTTATACTTGGGt                                                                                       >  2:630342/1‑138 (MQ=255)

                                                  gCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTa                                                                                     <  1:858333/140‑1 (MQ=255)

                                                  gCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTa                                                                                     <  2:260998/140‑1 (MQ=255)

                                                     gACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATc                                                                                  <  1:854365/140‑1 (MQ=255)

                                                      aCCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGt                                                                                       <  1:328745/134‑1 (MQ=255)

                                                      aCCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGt                                                                                       >  2:328745/1‑134 (MQ=255)

                                                      aCCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATcc                                                                                 <  2:1307688/140‑1 (MQ=255)

                                                       ccGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCg                                                                                <  1:1028338/140‑1 (MQ=255)

                                                       ccGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCg                                                                                <  1:592/140‑1 (MQ=255)

                                                        cGGTGCCGTCATCCGTGTCGCCGATATACATGCCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGt                                                                               >  2:1189040/1‑140 (MQ=255)

                                                        cGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGt                                                                               >  2:626232/1‑140 (MQ=255)

                                                          gTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGt                                                                                       <  1:158973/130‑1 (MQ=255)

                                                          gTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGt                                                                                       >  2:158973/1‑130 (MQ=255)

                                                           tGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTAt                                                                            >  1:1304808/1‑140 (MQ=255)

                                                            gCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTAtt                                                                           <  2:234654/140‑1 (MQ=255)

                                                             ccGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTGCGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTAttt                                                                          <  2:515987/140‑1 (MQ=255)

                                                                tCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTAc                                                                       >  1:673550/1‑140 (MQ=255)

                                                                 cATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTAcc                                                                      <  1:94905/140‑1 (MQ=255)

                                                                       tgtCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTc                                                                >  2:631331/1‑140 (MQ=255)

                                                                         tCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCa                                                              <  1:778608/140‑1 (MQ=255)

                                                                            ccGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTAttt                                                                          <  1:1257564/125‑1 (MQ=255)

                                                                            ccGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTAttt                                                                          >  2:667590/1‑125 (MQ=255)

                                                                            ccGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTAttt                                                                          <  1:667590/125‑1 (MQ=255)

                                                                            ccGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTAttt                                                                          >  2:1257564/1‑125 (MQ=255)

                                                                                       acCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTc                                                >  2:1198194/1‑140 (MQ=255)

                                                                                            ggCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAAtt                                           >  1:990106/1‑140 (MQ=255)

                                                                                            ggCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAAtt                                           >  1:1282177/1‑140 (MQ=255)

                                                                                              cgcTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAAtttt                                         >  1:756182/1‑140 (MQ=255)

                                                                                               gcTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTCTTCCACGGTAAACATCTTCGAATTTTc                                        >  1:1323533/1‑140 (MQ=255)

                                                                                                 ttACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGt                                      <  1:1322503/140‑1 (MQ=255)

                                                                                                 ttACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTACACGGTAAACATCTTCGAATTTTCGt                                      >  1:951885/1‑140 (MQ=255)

                                                                                                  tACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTc                                     >  2:775916/1‑140 (MQ=255)

                                                                                                  tACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTc                                     >  2:737096/1‑140 (MQ=255)

                                                                                                     gCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGa                                  <  2:990106/140‑1 (MQ=255)

                                                                                                     gCACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGa                                  <  2:1304808/140‑1 (MQ=255)

                                                                                                      cACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGAc                                 <  2:951885/140‑1 (MQ=255)

                                                                                                      cACCGCATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTCTTCCACGGTAAACATCTTCGAATTTTCGTCCGAc                                 <  2:1323533/140‑1 (MQ=255)

                                                                                                           cATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTcc                                    >  1:1194771/1‑132 (MQ=255)

                                                                                                           cATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTcc                                    <  1:723952/132‑1 (MQ=255)

                                                                                                           cATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTcc                                    >  2:723952/1‑132 (MQ=255)

                                                                                                           cATCCAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGATAAACATCTTCGAATTTTCGGcc                                    <  2:1194771/132‑1 (MQ=255)

                                                                                                               cAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGAc                                 >  1:906592/1‑131 (MQ=255)

                                                                                                               cAGCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGAc                                 <  2:906592/131‑1 (MQ=255)

                                                                                                                 gCCCTTTCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAAc                      <  2:582347/140‑1 (MQ=255)

                                                                                                                      ttCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAAc                      <  2:1246821/135‑1 (MQ=255)

                                                                                                                      ttCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAAc                      >  1:1246821/1‑135 (MQ=255)

                                                                                                                      ttCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGtt                 <  2:673550/140‑1 (MQ=255)

                                                                                                                       tCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGAc                                 <  1:1042212/123‑1 (MQ=255)

                                                                                                                       tCAGGACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGAc                                 >  2:1042212/1‑123 (MQ=255)

                                                                                                                          ggACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGc             <  1:1198194/140‑1 (MQ=255)

                                                                                                                          ggACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGc             <  1:737096/140‑1 (MQ=255)

                                                                                                                           gACTTTGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCa            <  1:775916/140‑1 (MQ=255)

                                                                                                                              tttGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTg         >  2:799643/1‑140 (MQ=255)

                                                                                                                                tGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGAt       >  1:174241/1‑140 (MQ=255)

                                                                                                                                tGATACTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGAt       >  2:1010111/1‑140 (MQ=255)

                                                                                                                                    aCTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATcgcg   <  1:626232/140‑1 (MQ=255)

                                                                                                                                    aCTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATcgcg   <  1:1189040/140‑1 (MQ=255)

                                                                                                                                     cTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCgcgc  <  2:261776/140‑1 (MQ=255)

                                                                                                                                     cTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCgcgc  <  2:174241/140‑1 (MQ=255)

                                                                                                                                     cTGAAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCgcgc  <  1:1010111/140‑1 (MQ=255)

CGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGC  >  NC_000913/3879961‑3880233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`