Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,736	A→G	T141A (ACC→GCC)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,736	0	A	G	100.0%	284.5 / NA	79	T141A (ACC→GCC)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/42);  total (37/42)

GCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGG  >  NC_000913/3931601‑3931866                                                                                                                                        |                                                                                                                                   gcgcGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCt                                                                                                                                 <  2:652666/139‑1 (MQ=255) gcgcGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCt                                                                                                                                 >  1:652666/1‑139 (MQ=255) gcgcGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTg                                                                                                                                <  2:1115875/140‑1 (MQ=255)  cgcgAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTgg                                                                                                                               >  1:1291375/1‑140 (MQ=255)  cgcgAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTgg                                                                                                                               >  1:191552/1‑140 (MQ=255)    cgAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGAt                                                                                                                             >  1:1216068/1‑140 (MQ=255)     gAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATa                                                                                                                            >  1:1269090/1‑140 (MQ=255)        ccACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGtt                                                                                                                         <  1:1104186/140‑1 (MQ=255)        ccACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGtt                                                                                                                         <  1:776677/140‑1 (MQ=255)           caTCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTccc                                                                                                                      >  1:1273054/1‑140 (MQ=255)           caTCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTccc                                                                                                                      <  1:1351502/140‑1 (MQ=255)             tCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCct                                                                                                                    <  2:448050/140‑1 (MQ=255)               aaTGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCctct                                                                                                                  >  2:587778/1‑140 (MQ=255)               aaTGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCctct                                                                                                                  <  2:1269090/140‑1 (MQ=255)                   cTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAAt                                                                                                              >  2:979965/1‑140 (MQ=255)                      gTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTGAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTAt                                                                                                           >  2:1181662/1‑140 (MQ=255)                      gTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTAt                                                                                                           >  1:899784/1‑140 (MQ=255)                          ttATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGtt                                                                                                       <  2:899784/140‑1 (MQ=255)                           tATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTtc                                                                                                      >  1:1106959/1‑140 (MQ=255)                           tATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTtc                                                                                                      <  1:42970/140‑1 (MQ=255)                            aTGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTtct                                                                                                     <  2:191552/140‑1 (MQ=255)                            aTGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTtct                                                                                                     <  1:979965/140‑1 (MQ=255)                            aTGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTtct                                                                                                     <  1:911115/140‑1 (MQ=255)                                   tAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTtta                                                                                              >  1:29941/1‑140 (MQ=255)                                   tAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTtta                                                                                              <  2:1291375/140‑1 (MQ=255)                                   tAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTtta                                                                                              <  2:116324/140‑1 (MQ=255)                                   tAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTtta                                                                                              <  2:1058328/140‑1 (MQ=255)                                    aaTCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTttat                                                                                             >  1:930153/1‑140 (MQ=255)                                     aTCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTa                                                                                                            >  2:1232065/1‑124 (MQ=255)                                     aTCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTa                                                                                                            <  1:1232065/124‑1 (MQ=255)                                     aTCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCa                                                                                                   <  2:141860/133‑1 (MQ=255)                                     aTCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCa                                                                                                   >  1:141860/1‑133 (MQ=255)                                     aTCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTttatt                                                                                            >  2:1184587/1‑140 (MQ=255)                                          cggcAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTAttattta                                                                                       >  1:1358016/1‑140 (MQ=255)                                          cggcAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTAttattta                                                                                       <  1:587778/140‑1 (MQ=255)                                           ggcAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTAttatttat                                                                                      <  1:484345/140‑1 (MQ=255)                                                 tttttCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCa                                                                                <  1:1181662/140‑1 (MQ=255)                                                    ttCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTAttatttat                                                                                      <  2:330230/131‑1 (MQ=255)                                                    ttCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTAttatttat                                                                                      >  1:330230/1‑131 (MQ=255)                                                            tGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCa                                                                     <  2:1216068/140‑1 (MQ=255)                                                              aaGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACg                                                                   >  1:779797/1‑140 (MQ=255)                                                              aaGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACg                                                                   >  1:37348/1‑140 (MQ=255)                                                                gtcgtcATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGc                                                                 >  1:635935/1‑140 (MQ=255)                                                                   gtcATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTAt                                                              <  1:1353583/140‑1 (MQ=255)                                                                    tcATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATg                                                             >  2:1200539/1‑140 (MQ=255)                                                                     cATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATgg                                                            >  1:871149/1‑140 (MQ=255)                                                                     cATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATgg                                                            >  2:824751/1‑140 (MQ=255)                                                                           acCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTa                                                      >  2:1095166/1‑140 (MQ=255)                                                                             ccGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATgg                                                            >  1:1286579/1‑132 (MQ=255)                                                                             ccGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATgg                                                            <  2:1286579/132‑1 (MQ=255)                                                                               gCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCt                                                  >  2:426912/1‑140 (MQ=255)                                                                                     tCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTgc                                            <  2:37348/140‑1 (MQ=255)                                                                                     tCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTgc                                            <  2:625808/140‑1 (MQ=255)                                                                                      cGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTgcg                                           <  2:1276379/140‑1 (MQ=255)                                                                                      cGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTgcg                                           <  2:930153/140‑1 (MQ=255)                                                                                                  ccATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTg                               >  2:362761/1‑140 (MQ=255)                                                                                                    aTTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGAc                             <  1:362761/140‑1 (MQ=255)                                                                                                     ttGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGAcc                            <  1:1176832/140‑1 (MQ=255)                                                                                                     ttGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGAcc                            <  2:1329490/140‑1 (MQ=255)                                                                                                      tGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCt                           >  1:908842/1‑140 (MQ=255)                                                                                                       gAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTg                          <  1:1200539/140‑1 (MQ=255)                                                                                                           gTCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGttt                      >  2:1327774/1‑140 (MQ=255)                                                                                                            tCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGtttt                     >  2:1011404/1‑140 (MQ=255)                                                                                                            tCTGGAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGtttt                     >  2:575186/1‑140 (MQ=255)                                                                                                                gAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGAt                 >  2:599166/1‑140 (MQ=255)                                                                                                                gAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGAt                 <  2:263341/140‑1 (MQ=255)                                                                                                                gAAATCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGAt                 <  2:92910/140‑1 (MQ=255)                                                                                                                 aaaTCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGAtt                <  2:1106959/140‑1 (MQ=255)                                                                                                                  aaTCGTCGCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTc               <  1:689700/140‑1 (MQ=255)                                                                                                                     cgtcgCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTgg            >  1:1015587/1‑140 (MQ=255)                                                                                                                     cgtcgCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTgg            <  1:426912/140‑1 (MQ=255)                                                                                                                     cgtcgCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTgg            <  1:52878/140‑1 (MQ=255)                                                                                                                      gtcgCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCCCGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACATGGTTTTTGATTCTGGc           >  2:1355780/1‑140 (MQ=255)                                                                                                                      gtcgCCCCGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGc           >  1:1355308/1‑140 (MQ=255)                                                                                                                           cccGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGAc      <  1:1184587/140‑1 (MQ=255)                                                                                                                           cccGCAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGAc      <  1:824751/140‑1 (MQ=255)                                                                                                                               cAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTggg  <  2:1358016/140‑1 (MQ=255)                                                                                                                               cAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTggg  <  2:871149/140‑1 (MQ=255)                                                                                                                               cAGCTGGATGCCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTggg  >  1:873271/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   GCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGG  >  NC_000913/3931601‑3931866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑