Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 490,933 A→G T18A (ACG→GCG)  ybaN → conserved inner membrane protein YbaN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913490,9330AG100.0% 168.6 / NA 50T18A (ACG→GCG) ybaNconserved inner membrane protein YbaN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/17);  total (33/17)

CGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACG  >  NC_000913/490803‑491063
                                                                                                                                   |                                                                                                                                  
cGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCA‑TTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCg                                                                                                                                  >  1:670082/1‑132 (MQ=255)
     ttCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTggta                                                                                                                      >  1:905870/1‑140 (MQ=255)
       cACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTggtatt                                                                                                                    <  1:860566/140‑1 (MQ=255)
       cACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTggtatt                                                                                                                    <  2:152730/140‑1 (MQ=255)
               gACCTCAATGAAAATGATAATTGTTATGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTggtattaccggtat                                                                                                            >  1:787149/1‑140 (MQ=255)
                    cAATGAAAATGATAATTGTTATGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGgtattaccggtattaccg                                                                                                       >  1:508240/1‑140 (MQ=255)
                       tGAAAATGATAATTGTTATGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACcgacg                                                                                                    >  1:49705/1‑140 (MQ=255)
                                      ttATGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCctg                                                                                     >  2:1182796/1‑140 (MQ=255)
                                        aTGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCTTGG‑AATAACGGGATTACCGGCGACGCCGGTTTTCctgctg                                                                                  >  1:835906/1‑140 (MQ=255)
                                        aTGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCctgct                                                                                   >  2:1043937/1‑140 (MQ=255)
                                          tcTAAAGTTAGCCACTCTGTAAGTTGACAACCCTCATTATGCAACGAACCTTTTTAACCACCATTGGCGGGTTGGCGGTAGTGTGGGGTGCG‑TGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTgg                                                                                 <  2:835906/139‑1 (MQ=255)
                                              aaG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTggcggc                                                                             >  2:1105172/1‑140 (MQ=255)
                                              aaG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTggcggc                                                                             >  1:125333/1‑140 (MQ=255)
                                               aG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGcc                                                                            >  2:1162749/1‑140 (MQ=255)
                                                    gCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGt                                                                        >  2:144971/1‑140 (MQ=255)
                                                         tctGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCttt                                                                   >  1:797190/1‑140 (MQ=255)
                                                           tGTAAGCTGACAACACTCTATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGc                                                                 <  2:49705/140‑1 (MQ=255)
                                                              aaGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCg                                                              >  2:828412/1‑140 (MQ=255)
                                                                   gACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGttctt                                                         >  1:289791/1‑140 (MQ=255)
                                                                   gACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCCGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGc                                                                 >  2:740905/1‑132 (MQ=255)
                                                                   gACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCCGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGc                                                                 <  1:740905/132‑1 (MQ=255)
                                                                    acaacaCTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGttcttc                                                        >  1:525004/1‑140 (MQ=255)
                                                                    acaacaCTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGttcttc                                                        >  1:139645/1‑140 (MQ=255)
                                                                    acaacaCTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGttcttc                                                        >  2:449342/1‑140 (MQ=255)
                                                                       acacTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTcccc                                                     >  2:141582/1‑140 (MQ=255)
                                                                       acacTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTcccc                                                     >  2:627403/1‑140 (MQ=255)
                                                                                    aaCGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCgcgc                                                 <  1:513590/131‑1 (MQ=255)
                                                                                    aaCGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCgcgc                                                 >  2:513590/1‑131 (MQ=255)
                                                                                     aCGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCt                                       <  2:73002/140‑1 (MQ=255)
                                                                                     aCGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCt                                       <  2:727607/140‑1 (MQ=255)
                                                                                     aCGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCt                                       <  1:828412/140‑1 (MQ=255)
                                                                                     aCGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCt                                       >  1:727607/1‑140 (MQ=255)
                                                                                     aCGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCt                                       <  1:1182796/140‑1 (MQ=255)
                                                                                     aCGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCt                                       <  1:1043937/140‑1 (MQ=255)
                                                                                         aTCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCcgc                                                   <  1:866621/124‑1 (MQ=255)
                                                                                         aTCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCcgc                                                   >  2:866621/1‑124 (MQ=255)
                                                                                               ttAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTa                             <  2:787149/140‑1 (MQ=255)
                                                                                                 aaTCATCATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTAcc                           <  1:141582/140‑1 (MQ=255)
                                                                                                  atcatcATTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCg                          >  2:544954/1‑140 (MQ=255)
                                                                                                   tcatcaTTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGc                         >  2:202443/1‑140 (MQ=255)
                                                                                                     atcatTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTc                       >  1:1332051/1‑140 (MQ=255)
                                                                                                        atTGGCTGGCTGGCGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATg                    >  2:481729/1‑140 (MQ=255)
                                                                                                                gctggcGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCa            >  2:868945/1‑140 (MQ=255)
                                                                                                                gctggcGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCa            <  2:1332051/140‑1 (MQ=255)
                                                                                                                 ctggcGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAg           <  2:125333/140‑1 (MQ=255)
                                                                                                                 ctggcGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAg           >  2:865514/1‑140 (MQ=255)
                                                                                                                 ctggcGGTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAg           <  1:1105172/140‑1 (MQ=255)
                                                                                                                      ggTAGTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATc      >  1:331631/1‑140 (MQ=255)
                                                                                                                          gTGCTGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACg  >  2:1032192/1‑140 (MQ=255)
                                                                                                                              tGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCt     >  2:1300991/1‑133 (MQ=255)
                                                                                                                              tGGGTGCGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCt     <  1:1300991/133‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                  
CGGTTTTCACGATAAGACCTCAATGAAAATGATAATTGTTATGCTAAAG‑TAGCCACTCTGTAAGCTGACAACACTCAATATGCAACGAATCATTTTAATCATCATTGGCTGGCTGGCGGTAGTGCTGGGTACGCTGGGCGTGGTATTACCGGTATTACCGACGACGCCGTTTATCCTGCTGGCGGCCTGGTGCTTTGCCCGTTCTTCCCCGCGCTTTCACGCCTGGTTGCTGTACCGCTCATGGTTTGGCAGCTATCTACG  >  NC_000913/490803‑491063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑