Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,232,933	C→T	A101T (GCG→ACG)	lysC ←	aspartate kinase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,232,933	0	C	T	100.0%	340.5 / NA	96	A101T (GCG→ACG)	lysC	aspartate kinase III
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/47);  total (49/47)

CACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTT  >  NC_000913/4232799‑4233067                                                                                                                                       |                                                                                                                                       cACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTg                                                                                                                                   >  1:740313/1‑140 (MQ=255)  aCTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGc                                                                                                                                  <  2:946018/140‑1 (MQ=255)  aCTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGc                                                                                                                                  <  2:341969/140‑1 (MQ=255)  aCTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCACCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGc                                                                                                                                  <  1:289233/140‑1 (MQ=255)   cTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGcc                                                                                                                                 >  2:149467/1‑140 (MQ=255)     ttACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAg                                                                                                                               <  2:218473/140‑1 (MQ=255)     ttACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAg                                                                                                                               <  2:828481/140‑1 (MQ=255)        cGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTg                                                                                                                                   >  1:23289/1‑133 (MQ=255)        cGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTg                                                                                                                                   <  2:23289/133‑1 (MQ=255)        cGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAAc                                                                                                                            >  2:849462/1‑140 (MQ=255)           aCATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGt                                                                                                                         >  1:1172159/1‑140 (MQ=255)             aTCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCt                                                                                                                                    <  1:625396/127‑1 (MQ=255)             aTCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCt                                                                                                                                    >  2:625396/1‑127 (MQ=255)             aTCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAg                                                                                                                               >  2:134950/1‑132 (MQ=255)             aTCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAg                                                                                                                               <  1:134950/132‑1 (MQ=255)                  aCCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTtc                                                                                                                  >  1:6831/1‑140 (MQ=255)                     aCTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTcc                                                                                                               <  2:1108779/140‑1 (MQ=255)                       tgtgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGGACAGTAATGTTCTCcag                                                                                                             >  2:1300427/1‑140 (MQ=255)                       tgtgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCcag                                                                                                             >  2:534197/1‑140 (MQ=255)                       tgtgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCcag                                                                                                             <  2:6831/140‑1 (MQ=255)                       tgtgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCcag                                                                                                             >  2:149854/1‑140 (MQ=255)                         tgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTGGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTACCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCcagca                                                                                                           >  2:935807/1‑140 (MQ=255)                         tgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCcagca                                                                                                           <  1:935807/140‑1 (MQ=255)                         tgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCcagca                                                                                                           <  1:1300427/140‑1 (MQ=255)                           ccTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCcagcag                                                                                                          <  2:1248203/139‑1 (MQ=255)                           ccTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCcagcag                                                                                                          >  1:1248203/1‑139 (MQ=255)                           ccTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGa                                                                                                         >  2:667841/1‑140 (MQ=255)                            cTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGAc                                                                                                        >  1:331286/1‑140 (MQ=255)                            cTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGAc                                                                                                        >  1:434960/1‑140 (MQ=255)                               aaCATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGtt                                                                                                     >  1:1328546/1‑140 (MQ=255)                                 cATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCATCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCa                                                                                                   >  2:976185/1‑140 (MQ=255)                                   tCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAAt                                                                                                 >  2:1192715/1‑140 (MQ=255)                                   tCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAAt                                                                                                 >  2:1158380/1‑140 (MQ=255)                                   tCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAAt                                                                                                 <  2:622034/140‑1 (MQ=255)                                   tCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAAt                                                                                                 <  1:667841/140‑1 (MQ=255)                                   tCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAAt                                                                                                 >  2:743814/1‑140 (MQ=255)                                   tCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAAt                                                                                                 <  2:745414/140‑1 (MQ=255)                                    cgcgTTCGCGCAGTATCTCAACAAACAGCCGGCTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAAtc                                                                                                >  1:508344/1‑140 (MQ=255)                                         tCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCAGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTc                                                                                           >  1:260371/1‑140 (MQ=255)                                          cgcgCAGGATCTCAACAAACAGCAGGGTCGACATCATCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCa                                                                                          <  1:976185/140‑1 (MQ=255)                                          cgcgCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCtt                                                                                            <  2:64619/138‑1 (MQ=255)                                          cgcgCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCtt                                                                                            >  1:64619/1‑138 (MQ=255)                                          cgcgCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCa                                                                                          <  2:1172159/140‑1 (MQ=255)                                          cgcgCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCa                                                                                          <  1:1333780/140‑1 (MQ=255)                                          cgcgCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCa                                                                                          <  1:556838/140‑1 (MQ=255)                                          cgcgCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCAGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCa                                                                                          <  2:260371/140‑1 (MQ=255)                                           gcgcAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCAc                                                                                         >  1:198565/1‑140 (MQ=255)                                               aGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGAt                                                                                     <  1:72495/140‑1 (MQ=255)                                               aGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGAt                                                                                     <  1:1158380/140‑1 (MQ=255)                                                ggATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATa                                                                                    >  1:38879/1‑140 (MQ=255)                                                 gATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATaa                                                                                   <  1:836591/140‑1 (MQ=255)                                                   tctcAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAAtct                                                                                               >  1:1350000/1‑126 (MQ=255)                                                   tctcAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAAtct                                                                                               <  2:1350000/126‑1 (MQ=255)                                                     tcAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCa                                                                                          <  2:465694/129‑1 (MQ=255)                                                     tcAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCa                                                                                          >  1:465694/1‑129 (MQ=255)                                                        acaaacaGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCgg                                                                            <  1:882549/140‑1 (MQ=255)                                                         caaacaGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACg                                                                                 >  1:178922/1‑134 (MQ=255)                                                         caaacaGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACg                                                                                 <  2:178922/134‑1 (MQ=255)                                                           aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGa                                                                                      <  1:1010273/127‑1 (MQ=255)                                                           aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGa                                                                                      >  2:1010273/1‑127 (MQ=255)                                                           aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTa                                                                         >  2:1097752/1‑140 (MQ=255)                                                           aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTa                                                                         >  2:606011/1‑140 (MQ=255)                                                           aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTa                                                                         >  2:177718/1‑140 (MQ=255)                                                             cagcagGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAAc                                                                       <  1:743814/140‑1 (MQ=255)                                                              agcagGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGGACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACg                                                                      >  2:5012/1‑140 (MQ=255)                                                              agcagGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACg                                                                      >  1:409940/1‑140 (MQ=255)                                                                cagGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCa                                                                    <  1:1192715/140‑1 (MQ=255)                                                                 agGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAg                                                                   <  2:1328546/140‑1 (MQ=255)                                                                  gggTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGa                                                                  <  2:113965/140‑1 (MQ=255)                                                                   ggTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGAc                                                                 <  1:149467/140‑1 (MQ=255)                                                                     tCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGt                                                               >  1:975693/1‑140 (MQ=255)                                                                          aTCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAg                                                          >  2:840732/1‑140 (MQ=255)                                                                             aGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAAt                                                       <  1:177718/140‑1 (MQ=255)                                                                                tCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGc                                                    <  1:849462/140‑1 (MQ=255)                                                                                tCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGc                                                    <  2:331286/140‑1 (MQ=255)                                                                                tCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTCCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGc                                                    <  2:409940/140‑1 (MQ=255)                                                                                   ccGTGGCTGACCAGCTCATCTGTCAGCGCCTGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCaaa                                                 >  1:204094/1‑140 (MQ=255)                                                                                   ccGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCaaa                                                 >  2:406781/1‑140 (MQ=255)                                                                                   ccGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCaaa                                                 >  2:154564/1‑140 (MQ=255)                                                                                       ggCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTgg                                             <  1:1320580/140‑1 (MQ=255)                                                                                         cTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGAt                                           >  2:625948/1‑140 (MQ=255)                                                                                            aCCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGtt                                        >  2:319886/1‑140 (MQ=255)                                                                                            aCCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGtt                                        >  1:1212393/1‑140 (MQ=255)                                                                                                 cTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGa                                   <  1:534197/140‑1 (MQ=255)                                                                                                 cTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGa                                   <  1:149854/140‑1 (MQ=255)                                                                                                   cATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATa                                 <  2:559667/140‑1 (MQ=255)                                                                                                       tGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGt                             <  1:1097752/140‑1 (MQ=255)                                                                                                        gTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTc                            >  1:454265/1‑140 (MQ=255)                                                                                                        gTCAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTc                            >  1:798/1‑140 (MQ=255)                                                                                                          cAGCGCCGGAGACGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGa                          <  1:840732/140‑1 (MQ=255)                                                                                                                   agaCGTTGCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGa                 >  1:415176/1‑140 (MQ=255)                                                                                                                          gCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAAtcgctc          >  1:1208105/1‑140 (MQ=255)                                                                                                                          gCCAGCGCCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAAtcgctc          >  1:206292/1‑140 (MQ=255)                                                                                                                              gcgcCGCCGTTTCTGCCCGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCGGACGTTCCAGAATGGCAATCTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCa      <  2:1212393/140‑1 (MQ=255)                                                                                                                              gcgcCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAAtcgctcgc        <  1:16461/138‑1 (MQ=255)                                                                                                                              gcgcCGCCGTTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAAtcgctcgc        >  2:16461/1‑138 (MQ=255)                                                                                                                               cgccgccgtTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAg     >  2:395361/1‑140 (MQ=255)                                                                                                                                  cgccgtTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGtt  >  2:141691/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       CACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTT  >  NC_000913/4232799‑4233067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑