Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,449,018	A→G	L109L (TTA→TTG)	chpB →	endoribonuclease toxin ChpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,449,018	0	A	G	100.0%	266.7 / NA	75	L109L (TTA→TTG)	chpB	endoribonuclease toxin ChpB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/38);  total (37/38)

CGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAA  >  NC_000913/4448883‑4449153                                                                                                                                        |                                                                                                                                        cGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCt                                                                                                                                     >  2:260292/1‑140 (MQ=255)  gTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCtt                                                                                                                                    >  2:887734/1‑140 (MQ=255)   ttCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTg                                                                                                                                   <  1:260292/140‑1 (MQ=255)       tttACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAgg                                                                                                                               <  1:887734/140‑1 (MQ=255)       tttACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAgg                                                                                                                               <  1:99919/140‑1 (MQ=255)        ttACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGc                                                                                                                              <  2:66384/140‑1 (MQ=255)          aCATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCgg                                                                                                                            <  1:573499/140‑1 (MQ=255)           cATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCggt                                                                                                                           >  1:858579/1‑140 (MQ=255)            aTTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCggtg                                                                                                                          <  2:888224/140‑1 (MQ=255)            aTTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCggtg                                                                                                                          <  2:771707/140‑1 (MQ=255)            aTTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCggtg                                                                                                                          <  1:1152706/140‑1 (MQ=255)              tGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCggtggt                                                                                                                        <  2:357449/140‑1 (MQ=255)              tGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCggtggg                                                                                                                        >  1:1291301/1‑139 (MQ=255)              tGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCggtggg                                                                                                                        >  2:385776/1‑139 (MQ=255)                cGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTGCACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGtggtgg                                                                                                                      >  1:416476/1‑140 (MQ=255)                cGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGtggtgg                                                                                                                      <  2:858579/140‑1 (MQ=255)                   agaagGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAgg                                                                                                                               >  1:760598/1‑128 (MQ=255)                   agaagGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAgg                                                                                                                               <  2:760598/128‑1 (MQ=255)                     aagGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATaa                                                                                                                 >  1:224561/1‑140 (MQ=255)                       ggCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAgg                                                                                                               <  2:718529/140‑1 (MQ=255)                       ggCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAgg                                                                                                               <  2:1291301/140‑1 (MQ=255)                        gCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAg                                                                                                                <  2:1249345/138‑1 (MQ=255)                        gCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAg                                                                                                                >  1:1249345/1‑138 (MQ=255)                        gCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTACTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAggg                                                                                                              >  2:1283070/1‑139 (MQ=255)                               gCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGGGGAATAAGGGGTGtttt                                                                                                       >  2:730469/1‑139 (MQ=255)                                   ggCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGtttattta                                                                                                   >  1:664830/1‑140 (MQ=255)                                      gtggtgCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATcg                                                                                                <  2:224561/140‑1 (MQ=255)                                      gtggtgCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATcg                                                                                                >  2:479019/1‑140 (MQ=255)                                        ggtgCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGGGGAATAAGGTGTGTTTTTTTTTcgcg                                                                                              >  2:1032567/1‑140 (MQ=255)                                            cTGGTGAATCAGGTGCGGATGATGGATCTGCACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCa                                                                                          <  2:416476/140‑1 (MQ=255)                                            cTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCa                                                                                          <  2:664830/140‑1 (MQ=255)                                            cTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCa                                                                                          <  1:1032567/140‑1 (MQ=255)                                               gTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGGGGAATAAGGTGGGTTTATTTATCGCGGGCATaa                                                                                       >  2:863054/1‑140 (MQ=255)                                               gTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGGGGAATAAGGGTTGTTTATTTATCGCGGGCATaa                                                                                       >  2:950933/1‑140 (MQ=255)                                                 gAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaa                                                                                     <  1:730469/140‑1 (MQ=255)                                                  aaTCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaa                                                                                    <  1:1301976/140‑1 (MQ=255)                                                    tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                  <  1:1128511/140‑1 (MQ=255)                                                    tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                  >  1:1345792/1‑140 (MQ=255)                                                    tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                  <  1:1105914/140‑1 (MQ=255)                                                    tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                  >  1:440309/1‑140 (MQ=255)                                                    tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                  <  1:459251/140‑1 (MQ=255)                                                    tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                  <  2:1345792/140‑1 (MQ=255)                                                    tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                  >  2:1128511/1‑140 (MQ=255)                                                    tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                  >  1:895787/1‑140 (MQ=255)                                                    tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                  >  1:757029/1‑140 (MQ=255)                                                    tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                  <  1:1041063/140‑1 (MQ=255)                                                    tCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGGGTGTTTATTTATCGCGGGCATaaaaaaa                                                                                  >  1:815604/1‑140 (MQ=255)                                                       ggTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAAccc                                                                               <  1:950933/140‑1 (MQ=255)                                                         tGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCtt                                                                             >  1:486914/1‑140 (MQ=255)                                                         tGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCtt                                                                             >  1:1101583/1‑140 (MQ=255)                                                         tGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCtt                                                                             >  2:753657/1‑140 (MQ=255)                                                           cGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTAc                                                                           <  1:479019/140‑1 (MQ=255)                                                                gatgGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGGATAAGGTGTGTTTATTTATCGCGGGCGTTAAAAAACCCTTACTTAcc                                                                      >  2:484033/1‑140 (MQ=255)                                                                        tACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCg                                                              <  1:484033/140‑1 (MQ=255)                                                                        tACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCg                                                              <  2:757029/140‑1 (MQ=255)                                                                        tACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCg                                                              <  2:440309/140‑1 (MQ=255)                                                                           acGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCg                                                           <  1:151317/140‑1 (MQ=255)                                                                              cccGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTc                                                        >  1:196802/1‑140 (MQ=255)                                                                               ccGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCg                                                              >  2:1020114/1‑133 (MQ=255)                                                                               ccGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCg                                                              <  1:1020114/133‑1 (MQ=255)                                                                               ccGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGtt                                                         >  2:723639/1‑138 (MQ=255)                                                                               ccGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGtt                                                         <  1:723639/138‑1 (MQ=255)                                                                               ccGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCa                                                       >  2:457858/1‑140 (MQ=255)                                                                                cGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAg                                                      <  1:457858/140‑1 (MQ=255)                                                                                 ggCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAgg                                                     <  1:753657/140‑1 (MQ=255)                                                                                  gCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAggg                                                    >  1:772171/1‑140 (MQ=255)                                                                                              tATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCag                                        <  2:196802/140‑1 (MQ=255)                                                                                                      tGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACtt                                >  2:1345952/1‑140 (MQ=255)                                                                                                        gCTGCGGATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACttat                              >  2:419799/1‑140 (MQ=255)                                                                                                             ggATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa                                   <  2:809700/130‑1 (MQ=255)                                                                                                             ggATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAgaagaa                                   >  1:809700/1‑130 (MQ=255)                                                                                                             ggATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTtatttatt                         >  2:214180/1‑140 (MQ=255)                                                                                                             ggATGAGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGGTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTtatttatt                         >  1:976833/1‑140 (MQ=255)                                                                                                                  aGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTg                    >  1:948634/1‑140 (MQ=255)                                                                                                                  aGGTGGTGGAAGAGGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTg                    <  2:948634/140‑1 (MQ=255)                                                                                                                              agGCGTTATTGCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAagg        >  1:296599/1‑140 (MQ=255)                                                                                                                                   ttattgCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCa   <  2:895787/140‑1 (MQ=255)                                                                                                                                    tattgCGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCaa  <  2:486914/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTGAATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGCGTTATTACGCTTGCAGGCGGTGGTGGAATAAGGTGTGTTTATTTATCGCGGGCATAAAAAAACCCTTACTAACCGAAGCCCGGCGTTCAGGGTTATTACGCCAGAAGAACTTATTTATTCTTTGCGCGCTCGAAGGAGGCAA  >  NC_000913/4448883‑4449153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑