Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,372	T→C	V226A (GTT→GCT)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,372	0	T	C	100.0%	174.1 / NA	49	V226A (GTT→GCT)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/27);  total (22/27)

AGCGGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTG  >  NC_000913/4637237‑4637508                                                                                                                                        |                                                                                                                                         agcgGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCct                                                                                                                                      <  1:288991/140‑1 (MQ=255)       gAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTcccc                                                                                                                                <  1:1206680/140‑1 (MQ=255)        aaTTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCg                                                                                                                               >  1:675198/1‑140 (MQ=255)        aaTTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCg                                                                                                                               <  1:520143/140‑1 (MQ=255)            ttATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGAtct                                                                                                                           <  2:630890/140‑1 (MQ=255)                  gCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGAt                                                                                                                             <  2:307948/132‑1 (MQ=255)                  gCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGAt                                                                                                                             >  1:307948/1‑132 (MQ=255)                  gCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGgtag                                                                                                                     >  2:1325440/1‑140 (MQ=255)                       cgcCATTTTGTTGGGGATTGCACTGGTTATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCg                                                                                                                >  1:1147025/1‑140 (MQ=255)                       cgcCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCg                                                                                                                >  2:812953/1‑140 (MQ=255)                       cgcCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCg                                                                                                                >  2:984586/1‑140 (MQ=255)                         ccATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAg                                                                                                              <  1:1325440/140‑1 (MQ=255)                                         tGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCgcgc                                                                                              >  1:448264/1‑140 (MQ=255)                                         tGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCgcgc                                                                                              >  2:540078/1‑140 (MQ=255)                                           cACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAg                                                                                            <  2:663120/140‑1 (MQ=255)                                            aCTGGTTATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAgg                                                                                           <  2:1147025/140‑1 (MQ=255)                                               ggTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGgcgc                                                                                        <  2:448264/140‑1 (MQ=255)                                                          cAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGgcgc                                                                                        <  1:468344/129‑1 (MQ=255)                                                          cAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGgcgc                                                                                        >  2:468344/1‑129 (MQ=255)                                                           aGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATgc                                                                            >  1:770154/1‑140 (MQ=255)                                                           aGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATgc                                                                            >  1:1014088/1‑140 (MQ=255)                                                               aTGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGt                                                                        <  2:405541/140‑1 (MQ=255)                                                                  gTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGaa                                                                     >  2:595825/1‑140 (MQ=255)                                                                            tCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAggg                                                           <  2:1350033/140‑1 (MQ=255)                                                                            tCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAggg                                                           <  2:779433/140‑1 (MQ=255)                                                                            tCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAggg                                                           >  2:860370/1‑140 (MQ=255)                                                                             cAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGa                                                          >  1:335368/1‑140 (MQ=255)                                                                              aaCCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGaa                                                         <  1:1182148/140‑1 (MQ=255)                                                                                 cGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGaaaaa                                                      <  2:675198/140‑1 (MQ=255)                                                                                 cGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGaaaaa                                                      <  2:1014088/140‑1 (MQ=255)                                                                                  gCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGaa                                                              >  2:106198/1‑131 (MQ=255)                                                                                  gCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGaa                                                              <  1:106198/131‑1 (MQ=255)                                                                                           cccGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGaa                                                         <  1:767919/127‑1 (MQ=255)                                                                                           cccGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGaa                                                         >  2:767919/1‑127 (MQ=255)                                                                                           cccGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAg                                            <  1:860370/140‑1 (MQ=255)                                                                                           cccGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAg                                            <  1:127833/140‑1 (MQ=255)                                                                                            ccGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGc                                           <  2:885473/140‑1 (MQ=255)                                                                                                cTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAg                                            <  2:165873/135‑1 (MQ=255)                                                                                                cTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAg                                            >  1:165873/1‑135 (MQ=255)                                                                                                    cTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGtt                                   <  1:984586/140‑1 (MQ=255)                                                                                                          aTGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAg                                            >  1:978189/1‑125 (MQ=255)                                                                                                          aTGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAg                                            <  2:978189/125‑1 (MQ=255)                                                                                                          aTGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGt                                        >  2:150996/1‑129 (MQ=255)                                                                                                          aTGCTGATTCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGt                                        <  1:150996/129‑1 (MQ=255)                                                                                                                 ttCCGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTc                      >  1:857698/1‑140 (MQ=255)                                                                                                                   ccGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGtt                                   >  2:246860/1‑125 (MQ=255)                                                                                                                   ccGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGtt                                   <  1:246860/125‑1 (MQ=255)                                                                                                                   ccGTCACTGACAATAAGCCCGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCAt                    >  2:698541/1‑140 (MQ=255)                                                                                                                                     ccGCTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTg  <  1:698541/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         AGCGGCGAATTTTATGGGCCAGCGCCATTTTGTTGGGGATTGCACTGGTGATTGGCGCAGGCATGGTTTGGTGGATCAACCGCTCTATTGCCCGGCTCACTCGCTATGCTGATTCCGTCACTGACAATAAGCCCGTTCCTCTCCCCGATCTCGGTAGTAGCGAGTTGCGTAAACTCGCGCAGGCGCTGGAAAGTATGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTG  >  NC_000913/4637237‑4637508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑