Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	554,473	C→T	intergenic (‑36/‑138)	ppiB ← / → cysS	peptidyl‑prolyl cis‑trans isomerase B/cysteine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	554,473	0	C	T	100.0%	259.6 / NA	73	intergenic (‑36/‑138)	ppiB/cysS	peptidyl‑prolyl cis‑trans isomerase B/cysteine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/41);  total (32/41)

CCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCAATTACCCACACATGTCTAAACG  >  NC_000913/554337‑554601                                                                                                                                         |                                                                                                                                 ccTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAg                                                                                                                               >  2:603747/1‑140 (MQ=255) ccTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAg                                                                                                                               >  2:174672/1‑140 (MQ=255)  cTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGt                                                                                                                              >  2:802265/1‑140 (MQ=255)  cTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGt                                                                                                                              >  2:808985/1‑140 (MQ=255)   ttCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGtt                                                                                                                             >  2:1118065/1‑140 (MQ=255)    tCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTc                                                                                                                            <  1:652612/140‑1 (MQ=255)    tCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTc                                                                                                                            <  1:1305143/140‑1 (MQ=255)     cgcgGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCg                                                                                                                           >  2:224145/1‑140 (MQ=255)      gcgGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCgg                                                                                                                          <  2:1095227/140‑1 (MQ=255)       cgGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCggg                                                                                                                         <  2:698548/140‑1 (MQ=255)       cgGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCACTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCggg                                                                                                                         <  2:935293/140‑1 (MQ=255)        ggCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGt                                                                                                                        <  2:194033/140‑1 (MQ=255)         gCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTc                                                                                                                       <  2:154802/140‑1 (MQ=255)         gCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTc                                                                                                                       <  1:259043/140‑1 (MQ=255)         gCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTc                                                                                                                       <  2:871280/140‑1 (MQ=255)            gtagtCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCata                                                                                                                    >  2:372468/1‑140 (MQ=255)                 ccAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAg                                                                                                               <  1:174672/140‑1 (MQ=255)                 ccAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAg                                                                                                               <  1:603747/140‑1 (MQ=255)                   aGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAggg                                                                                                             <  2:1119054/140‑1 (MQ=255)                    ggAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAggg                                                                                                             >  2:390755/1‑139 (MQ=255)                    ggAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAggg                                                                                                             <  1:390755/139‑1 (MQ=255)                    ggAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGggt                                                                                                            <  1:808985/140‑1 (MQ=255)                      aaGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGggtgg                                                                                                          <  1:356317/140‑1 (MQ=255)                      aaGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGggtgg                                                                                                          >  1:211869/1‑140 (MQ=255)                         tttttAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGgtgt                                                                                                       <  1:224145/140‑1 (MQ=255)                          ttttAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGtt                                                                                                      >  1:366462/1‑140 (MQ=255)                           tttAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTta                                                                                                     <  1:372468/140‑1 (MQ=255)                           tttAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTta                                                                                                     >  1:856146/1‑140 (MQ=255)                            ttAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTtat                                                                                                    >  1:712349/1‑140 (MQ=255)                                     tCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAAcc                                                                                           >  1:766030/1‑140 (MQ=255)                                      cAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCg                                                                                          <  1:1114629/140‑1 (MQ=255)                                            cTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGAt                                                                                    >  1:26548/1‑140 (MQ=255)                                             tttATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATc                                                                                   >  2:1080923/1‑140 (MQ=255)                                             tttATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATc                                                                                   >  1:759859/1‑140 (MQ=255)                                              ttATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCg                                                                                  <  2:371298/140‑1 (MQ=255)                                                  cGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGatc                                                                              >  1:95329/1‑140 (MQ=255)                                                    tCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGGGGTGTTATAGCATAACCGCACGATCGGatcat                                                                            >  2:369117/1‑140 (MQ=255)                                                       aaaGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCAc                                                                         <  1:1080923/140‑1 (MQ=255)                                                       aaaGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCAc                                                                         <  2:466638/140‑1 (MQ=255)                                                       aaaGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCAc                                                                         <  1:641792/140‑1 (MQ=255)                                                        aaGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACg                                                                        <  2:1284517/140‑1 (MQ=255)                                                        aaGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACg                                                                        <  2:211869/140‑1 (MQ=255)                                                                      atatCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGAt                                                          <  1:1118065/140‑1 (MQ=255)                                                                           gCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTcgcg                                                     <  2:80278/140‑1 (MQ=255)                                                                             cGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTcgcgcg                                                   <  2:856146/140‑1 (MQ=255)                                                                                   tGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAat                                             <  2:1346387/140‑1 (MQ=255)                                                                                    ggTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAata                                            <  2:1859/140‑1 (MQ=255)                                                                                       gtgGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATgg                                         <  2:865755/140‑1 (MQ=255)                                                                                       gtgGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATgg                                         >  2:1046268/1‑140 (MQ=255)                                                                                        tgGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTCCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATggg                                        >  2:1199536/1‑140 (MQ=255)                                                                                             aGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGtatta                                   >  2:259661/1‑140 (MQ=255)                                                                                             aGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGtatta                                   >  2:1150694/1‑140 (MQ=255)                                                                                               tAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATtata                                 <  1:1046268/140‑1 (MQ=255)                                                                                               tAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATtata                                 >  2:687989/1‑140 (MQ=255)                                                                                               tAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATtata                                 <  2:26548/140‑1 (MQ=255)                                                                                                  ccATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGc                              >  2:953089/1‑140 (MQ=255)                                                                                                  ccATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGc                              >  1:1151656/1‑140 (MQ=255)                                                                                                  ccATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCACACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGc                              >  1:762834/1‑140 (MQ=255)                                                                                                      ttttGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACt                          <  1:259661/140‑1 (MQ=255)                                                                                                       tttGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTc                         >  1:145965/1‑140 (MQ=255)                                                                                                        ttGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCa                        <  1:687989/140‑1 (MQ=255)                                                                                                         tGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCaa                       <  2:1322394/140‑1 (MQ=255)                                                                                                         tGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCaa                       >  1:1322394/1‑140 (MQ=255)                                                                                                         tGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCaa                       <  2:619800/140‑1 (MQ=255)                                                                                                         tGCATCCTGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCaa                       <  1:953089/140‑1 (MQ=255)                                                                                                                tGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCAATTACCca                >  2:378087/1‑140 (MQ=255)                                                                                                                tGTTCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCAATTACCca                >  1:634762/1‑140 (MQ=255)                                                                                                                  ttCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCAATTACCcaca              >  2:919356/1‑140 (MQ=255)                                                                                                                   tCCGTTTGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCAATTACCcacac             <  2:581699/140‑1 (MQ=255)                                                                                                                         tGATTGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCAATTACCCACACATGTCt       >  1:1009354/1‑140 (MQ=255)                                                                                                                            ttGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCAATTACCCACACATGTCTaaa    <  2:366462/140‑1 (MQ=255)                                                                                                                             tGGTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCAATTACCCACACATGTCTAAAc   <  2:1009354/140‑1 (MQ=255)                                                                                                                              ggTGCTTCAACTCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCACACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCAATTACCCACACATGTCTAAACg  <  2:762834/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 CCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGTCATATATAGGGTGGTGTTATAGCATAACCGCACGATCGGATCATCACGCAATGTATGCTGATTCGCGCGGGAAATATGGGTATTATACGCAACTCAATTACCCACACATGTCTAAACG  >  NC_000913/554337‑554601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑