Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	812,758	A→G	I163M (ATA→ATG)	bioD →	dethiobiotin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	812,758	0	A	G	100.0%	320.3 / NA	88	I163M (ATA→ATG)	bioD	dethiobiotin synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/39);  total (49/39)

GCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCA  >  NC_000913/812621‑812893                                                                                                                                          |                                                                                                                                        gCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATgca                                                                                                                                       >  1:606995/1‑140 (MQ=255)  cTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATgcaa                                                                                                                                      >  1:1234806/1‑140 (MQ=255)      cggcTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACg                                                                                                                                  >  1:7686/1‑140 (MQ=255)          tGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCgg                                                                                                                              >  1:1037839/1‑140 (MQ=255)          tGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCgg                                                                                                                              >  1:1333547/1‑140 (MQ=255)             tttACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGgact                                                                                                                           <  2:11259/140‑1 (MQ=255)               tACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGgactga                                                                                                                         <  2:7686/140‑1 (MQ=255)                    cgcTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTg                                                                                                                    <  1:1212730/140‑1 (MQ=255)                    cgcTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTg                                                                                                                    <  1:1105858/140‑1 (MQ=255)                         tCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCggg                                                                                                               >  1:1123039/1‑140 (MQ=255)                               actttcactttTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGt                                                                                                         <  2:282499/140‑1 (MQ=255)                                   tcactttTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGActct                                                                                                                     <  2:1219890/124‑1 (MQ=255)                                   tcactttTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGActct                                                                                                                     >  1:1219890/1‑124 (MQ=255)                                   tcactttTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGt                                                                                                              <  2:291890/131‑1 (MQ=255)                                   tcactttTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGt                                                                                                              >  1:291890/1‑131 (MQ=255)                                     actttTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGaa                                                                                                   <  1:679780/140‑1 (MQ=255)                                       ttttGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACg                                                                                                 >  2:577753/1‑140 (MQ=255)                                             aGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTa                                                                                           <  2:245386/140‑1 (MQ=255)                                                         acaGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGcc                                                                                       <  2:192159/132‑1 (MQ=255)                                                         acaGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGcc                                                                                       >  1:192159/1‑132 (MQ=255)                                                           aGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGaaaa                                                                             >  2:1134921/1‑140 (MQ=255)                                                           aGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGaaaa                                                                             <  2:1123039/140‑1 (MQ=255)                                                           aGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGaaaa                                                                             >  2:1321715/1‑140 (MQ=255)                                                           aGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGaaaa                                                                             >  2:157424/1‑140 (MQ=255)                                                             gAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGa                                                                                >  2:524044/1‑135 (MQ=255)                                                             gAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGa                                                                                <  1:524044/135‑1 (MQ=255)                                                             gAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACg                                                                           >  2:1148124/1‑140 (MQ=255)                                                             gAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACg                                                                           >  2:100883/1‑140 (MQ=255)                                                             gAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACg                                                                           >  1:889954/1‑140 (MQ=255)                                                             gAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACg                                                                           >  2:984001/1‑140 (MQ=255)                                                             gAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACg                                                                           <  1:1188574/140‑1 (MQ=255)                                                                caacTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCa                                                                        <  1:1134921/140‑1 (MQ=255)                                                                caacTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCa                                                                        <  2:138809/140‑1 (MQ=255)                                                                caacTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCa                                                                        <  1:984001/140‑1 (MQ=255)                                                                  acTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACg                                                                      <  2:1333547/140‑1 (MQ=255)                                                                  acTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACg                                                                      <  1:577753/140‑1 (MQ=255)                                                                   cTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGc                                                                     >  1:757408/1‑140 (MQ=255)                                                                   cTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGc                                                                     >  2:197506/1‑140 (MQ=255)                                                                    tGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCt                                                                    <  1:100883/140‑1 (MQ=255)                                                                    tGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCt                                                                    <  2:757408/140‑1 (MQ=255)                                                                    tGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCt                                                                    <  2:486393/140‑1 (MQ=255)                                                                     gCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTg                                                                   >  2:702169/1‑140 (MQ=255)                                                                       cGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACg                                                                      <  1:353486/135‑1 (MQ=255)                                                                       cGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACg                                                                      >  2:353486/1‑135 (MQ=255)                                                                          tGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAatat                                                              <  1:1148124/140‑1 (MQ=255)                                                                           gATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAatata                                                             <  1:157424/140‑1 (MQ=255)                                                                            aTACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAatatat                                                            >  2:1341320/1‑140 (MQ=255)                                                                                   tAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACg                                                     >  2:164737/1‑140 (MQ=255)                                                                                    aGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAatat                                                              <  1:162672/130‑1 (MQ=255)                                                                                    aGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAatat                                                              >  2:162672/1‑130 (MQ=255)                                                                                     gTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCt                                                   <  1:805170/140‑1 (MQ=255)                                                                                     gTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCt                                                   <  2:1234806/140‑1 (MQ=255)                                                                                        ggTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCt                                                   >  1:1109118/1‑137 (MQ=255)                                                                                        ggTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCt                                                   <  2:1109118/137‑1 (MQ=255)                                                                                         gtgtGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCAcc                                               >  2:1015521/1‑140 (MQ=255)                                                                                          tgtgAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCAccc                                              >  2:257136/1‑140 (MQ=255)                                                                                          tgtgAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCAccc                                              >  1:384629/1‑140 (MQ=255)                                                                                            tgAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGc                                            >  1:638470/1‑140 (MQ=255)                                                                                            tgAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGc                                            >  1:1041976/1‑140 (MQ=255)                                                                                            tgAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGc                                            >  2:767077/1‑140 (MQ=255)                                                                                             gAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCa                                           >  2:57443/1‑140 (MQ=255)                                                                                               aaCTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATg                                         >  1:873205/1‑140 (MQ=255)                                                                                                aCTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATGTAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGa                                        >  2:1075116/1‑140 (MQ=255)                                                                                                      cTGTATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCcg                                  >  2:303992/1‑140 (MQ=255)                                                                                                         tATTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCgcgc                               <  1:702169/140‑1 (MQ=255)                                                                                                          aTTAATCACGCGATGTTGACTGCACAGGTAATGTAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGcgcc                              <  1:1075116/140‑1 (MQ=255)                                                                                                          aTTAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGcgcc                              <  1:57443/140‑1 (MQ=255)                                                                                                           ttAATCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCTGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGcgccg                             <  1:257136/140‑1 (MQ=255)                                                                                                              aTCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCgctg                          >  2:442163/1‑140 (MQ=255)                                                                                                              aTCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCgctg                          >  2:1121419/1‑140 (MQ=255)                                                                                                              aTCACGCGATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCgctg                          <  2:889954/140‑1 (MQ=255)                                                                                                                  cgcgATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTgg                      <  2:1241134/140‑1 (MQ=255)                                                                                                                   gcgATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTggg                     >  1:1274581/1‑140 (MQ=255)                                                                                                                   gcgATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTggg                     >  1:955126/1‑140 (MQ=255)                                                                                                                   gcgATGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTggg                     >  2:571470/1‑140 (MQ=255)                                                                                                                       tGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGgagag                 <  2:1274581/140‑1 (MQ=255)                                                                                                                       tGTTGACTGCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGgagag                 <  1:769349/140‑1 (MQ=255)                                                                                                                               gCACAGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTg         >  2:1231040/1‑140 (MQ=255)                                                                                                                                cacaGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCgctg                          <  2:259160/122‑1 (MQ=255)                                                                                                                                cacaGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCgctg                          >  1:259160/1‑122 (MQ=255)                                                                                                                                cacaGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTgg        >  1:816737/1‑140 (MQ=255)                                                                                                                                 acaGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGc       >  1:294545/1‑140 (MQ=255)                                                                                                                                 acaGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGc       >  2:779845/1‑140 (MQ=255)                                                                                                                                   aGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGgag                   >  2:510231/1‑126 (MQ=255)                                                                                                                                   aGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGgag                   <  1:510231/126‑1 (MQ=255)                                                                                                                                   aGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCtt     <  1:571470/140‑1 (MQ=255)                                                                                                                                   aGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCtt     >  2:281796/1‑140 (MQ=255)                                                                                                                                   aGGTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCtt     >  2:161317/1‑140 (MQ=255)                                                                                                                                     gTAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGc   >  2:1085542/1‑140 (MQ=255)                                                                                                                                      tAATGCAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCa  >  1:167346/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GCTGGCGGCTGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCA  >  NC_000913/812621‑812893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑