Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	915,074	C→G	intergenic (‑217/+278)	lysO ← / ← aqpZ	L‑lysine exporter/water channel AqpZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	915,074	0	C	G	100.0%	171.0 / NA	54	intergenic (‑217/+278)	lysO/aqpZ	L‑lysine exporter/water channel AqpZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (27/27);  total (27/27)

TAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGG  >  NC_000913/914939‑915207                                                                                                                                        |                                                                                                                                      tAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGcaca                                                                                                                                   <  1:908420/140‑1 (MQ=255) tAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGcaca                                                                                                                                   <  2:1333372/140‑1 (MQ=255)     aGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGa                                                                                                                               <  2:669337/140‑1 (MQ=255)                   aTAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGgc                                                                                                                 <  1:1204587/140‑1 (MQ=255)                    tAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGgcg                                                                                                                <  2:183454/140‑1 (MQ=255)                      aGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAACTCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGcgcg                                                                                                              >  2:1190647/1‑140 (MQ=255)                      aGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGcgcg                                                                                                              <  2:789379/140‑1 (MQ=255)                      aGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGcgcg                                                                                                              <  2:1094574/140‑1 (MQ=255)                       ggAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGt                                                                                                             >  2:618404/1‑140 (MQ=255)                           cAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTa                                                                                                         >  2:743375/1‑140 (MQ=255)                            aGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTAc                                                                                                        >  1:1171196/1‑140 (MQ=255)                            aGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTAc                                                                                                        >  1:250862/1‑140 (MQ=255)                              tAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAg                                                                                                      <  1:743375/140‑1 (MQ=255)                                 agTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGa                                                                                                   >  1:942473/1‑140 (MQ=255)                                 agTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGa                                                                                                   >  1:315594/1‑140 (MQ=255)                                   tATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAg                                                                                                      >  2:344317/1‑135 (MQ=255)                                   tATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAg                                                                                                      <  1:344317/135‑1 (MQ=255)                                     ttCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGtt                                                                                               >  2:398671/1‑140 (MQ=255)                                     ttCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGtt                                                                                               >  1:114648/1‑140 (MQ=255)                                      tCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTAc                                                                                                        >  2:21903/1‑130 (MQ=255)                                      tCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTAc                                                                                                        <  1:21903/130‑1 (MQ=255)                                                  aaaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGa                                                                                  <  1:398671/140‑1 (MQ=255)                                                  aaaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGa                                                                                  <  2:1023583/140‑1 (MQ=255)                                                  aaaGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGa                                                                                  >  1:1023583/1‑140 (MQ=255)                                                       tatTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCgg                                                                             <  1:1039567/140‑1 (MQ=255)                                                        atTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGt                                                                            <  2:250862/140‑1 (MQ=255)                                                              gcgcTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAg                                                                          <  2:929248/136‑1 (MQ=255)                                                              gcgcTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAg                                                                          >  1:929248/1‑136 (MQ=255)                                                                gcTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTaa                                                                    <  1:960566/140‑1 (MQ=255)                                                                 cTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACAAGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAg                                                                   >  2:719797/1‑140 (MQ=255)                                                                    tAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGa                                                                >  1:1038293/1‑140 (MQ=255)                                                                     aaTTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAt                                                               <  2:114648/140‑1 (MQ=255)                                                                                 cACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCa                                                   >  1:980965/1‑140 (MQ=255)                                                                                 cACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCa                                                   >  2:732895/1‑140 (MQ=255)                                                                                 cACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCa                                                   <  1:732895/140‑1 (MQ=255)                                                                                  aCCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAt                                                               <  1:792684/127‑1 (MQ=255)                                                                                  aCCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGAt                                                               >  2:792684/1‑127 (MQ=255)                                                                                         ttGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCgcgc                                             >  1:607015/1‑138 (MQ=25)                                                                                         ttGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCgcgc                                             <  2:607015/138‑1 (MQ=25)                                                                                              ggCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAgcggcg                                      >  1:406305/1‑140 (MQ=25)                                                                                              ggCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAgcggcg                                      >  1:272399/1‑140 (MQ=21)                                                                                              ggCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAgcggcg                                      >  2:366944/1‑140 (MQ=25)                                                                                                           ccGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCa                         <  1:366944/140‑1 (MQ=17)                                                                                                           ccGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCa                         >  1:341043/1‑140 (MQ=17)                                                                                                           ccGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCa                         <  2:1171196/140‑1 (MQ=17)                                                                                                           ccGAGGCGTTCCCTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTAGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCa                         <  1:1190647/140‑1 (MQ=17)                                                                                                                     cccTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCgggg               <  1:242839/140‑1 (MQ=17)                                                                                                                       cTGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGATGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGt             <  1:180123/140‑1 (MQ=17)                                                                                                                        tGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTg            >  1:1099332/1‑140 (MQ=17)                                                                                                                        tGAAGGCCGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTg            >  1:881455/1‑140 (MQ=17)                                                                                                                               cGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCg                  <  2:1216723/127‑1 (MQ=17)                                                                                                                               cGGGGCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCg                  >  1:1216723/1‑127 (MQ=17)                                                                                                                                ggggCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggc    >  2:49160/1‑140 (MQ=17)                                                                                                                                  ggCAGCGCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggcgg  <  2:517962/140‑1 (MQ=17)                                                                                                                                        |                                                                                                                                      TAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGGCCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCACCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGCGG  >  NC_000913/914939‑915207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑