Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 916,237 A→G intergenic (‑190/‑236) aqpZ ← / → ybjD water channel AqpZ/DUF2813 domain‑containing protein YbjD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913916,2370AG100.0% 163.8 / NA 46intergenic (‑190/‑236)aqpZ/ybjDwater channel AqpZ/DUF2813 domain‑containing protein YbjD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/17);  total (29/17)

CGTCGTAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGACAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCATTTTT  >  NC_000913/916101‑916370
                                                                                                                                        |                                                                                                                                     
cgtcgtAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCa                                                                                                                                     >  1:1205754/1‑139 (MQ=255)
  tcgtAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAgtg                                                                                                                                  >  2:890339/1‑140 (MQ=255)
  tcgtAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAgtg                                                                                                                                  >  1:1150066/1‑140 (MQ=255)
  tcgtAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAgtg                                                                                                                                  >  1:138110/1‑140 (MQ=255)
       aaaaaGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATTAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTg                                                                                                                             <  1:674503/140‑1 (MQ=255)
               acgacgGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCaaa                                                                                                                     <  1:1349409/140‑1 (MQ=255)
                      aaaTAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTa                                                                                                              <  2:802737/140‑1 (MQ=255)
                                cgGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAgg                                                                                                    >  2:1335411/1‑140 (MQ=255)
                                cgGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAgg                                                                                                    >  1:604250/1‑140 (MQ=255)
                                cgGCTATTTTAAAAACGAAGGCGAGTCATTCA‑CAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGc                                                                                                   >  2:875766/1‑140 (MQ=255)
                                  gCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCg                                                                                                  <  2:870855/140‑1 (MQ=255)
                                      ttttAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGtaat                                                                                              >  2:650004/1‑140 (MQ=255)
                                                gAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAaataat                                                                                    >  2:1117189/1‑140 (MQ=255)
                                                    gCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTAt                                                                                <  1:1335411/140‑1 (MQ=255)
                                                     cGAGTCATTCA‑CAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCa                                                                              <  1:875766/140‑1 (MQ=255)
                                                                 cAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAattatt                                                                   <  2:604250/140‑1 (MQ=255)
                                                                    ataaataaATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGa                                                                >  2:657202/1‑140 (MQ=255)
                                                                    ataaataaATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGa                                                                <  1:650004/140‑1 (MQ=255)
                                                                     taaataaaTCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGAt                                                               >  2:1032113/1‑140 (MQ=255)
                                                                        ataaatCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGAtttt                                                            >  1:1263496/1‑140 (MQ=255)
                                                                        ataaatCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGAtttt                                                            >  2:563510/1‑140 (MQ=255)
                                                                         taaatCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTttg                                                           <  1:563510/140‑1 (MQ=255)
                                                                         taaatCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTttg                                                           >  2:501812/1‑140 (MQ=255)
                                                                           aatCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTttgtt                                                         >  1:715240/1‑140 (MQ=255)
                                                                             tCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTtgttgt                                                       >  1:478286/1‑140 (MQ=255)
                                                                             tCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTtgttgt                                                       >  1:1115480/1‑140 (MQ=255)
                                                                                  tAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTg                                                  >  2:111203/1‑140 (MQ=255)
                                                                                    aaTTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCa                                                <  1:1117189/140‑1 (MQ=255)
                                                                                      tttGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGa                                              <  1:364502/140‑1 (MQ=255)
                                                                                       ttGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGAc                                             <  1:890339/140‑1 (MQ=255)
                                                                                                ggCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCa                                    <  1:612079/140‑1 (MQ=255)
                                                                                                ggCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCa                                    <  1:1032113/140‑1 (MQ=255)
                                                                                                    aCAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAagag                                >  2:1242391/1‑140 (MQ=255)
                                                                                                            ttGCCCCATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGtata                        <  2:1263496/140‑1 (MQ=255)
                                                                                                               ccccATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAAcg                     >  1:658146/1‑140 (MQ=255)
                                                                                                                cccATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCa                                    <  1:1183530/124‑1 (MQ=255)
                                                                                                                cccATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCa                                    >  2:1183530/1‑124 (MQ=255)
                                                                                                                  cATATAGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGa                  >  1:405094/1‑140 (MQ=255)
                                                                                                                    tataGTCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGatt                >  1:718856/1‑140 (MQ=255)
                                                                                                                         tCATTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCg           >  2:28114/1‑140 (MQ=255)
                                                                                                                           aTTTGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCt         >  2:774187/1‑140 (MQ=255)
                                                                                                                            tttGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTc        >  1:763557/1‑140 (MQ=255)
                                                                                                                              tGTCTGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCAt      >  2:700088/1‑140 (MQ=255)
                                                                                                                                  tGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCAttttt  >  1:489779/1‑140 (MQ=255)
                                                                                                                                  tGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCAttttt  <  1:202740/140‑1 (MQ=255)
                                                                                                                                  tGATTGGCAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCAttttt  >  1:1047002/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                     
CGTCGTAAAAAAGTGACGACGGAAATAATGCGCGGCTATTTTAAAAACGAAGGCGAGTCATTCACCAGATAAATAAATCCAGTAAATTTGATTTAGGGCAACAGCGGGTTGCCCCATATAGTCATTTGTCTGATTGACAGTGTAGTGCACGCAAAAGATTTAATCCTTTAGGCGTAATAAAAAATAATTTATCATGCTAATTATTTGATTTTGTTGTTTTTGCAGACTTATCAGCAAGAGGGAGTATAACGCGATTATTCGCTCATTTTT  >  NC_000913/916101‑916370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑