Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,133,367	C→T	S265F (TCC→TTC)	flgE →	flagellar hook protein FlgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,133,367	0	C	T	100.0%	283.7 / NA	80	S265F (TCC→TTC)	flgE	flagellar hook protein FlgE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/41);  total (39/41)

GCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGCAACTATTCCAACGAACA  >  NC_000913/1133232‑1133493                                                                                                                                        |                                                                                                                               gCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATg                                                                                                                            <  2:942012/140‑1 (MQ=255)  cATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATgc                                                                                                                           <  2:543029/140‑1 (MQ=255)    ttGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCa                                                                                                                              >  1:54667/1‑135 (MQ=255)    ttGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATgcag                                                                                                                         >  1:443952/1‑140 (MQ=255)    ttGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATgcag                                                                                                                         >  2:901016/1‑140 (MQ=255)    ttGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATgcag                                                                                                                         >  1:866903/1‑140 (MQ=255)    ttGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATgcag                                                                                                                         >  2:1291284/1‑140 (MQ=255)    ttGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATgcag                                                                                                                         >  2:1295695/1‑140 (MQ=255)          aGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATgc                                                                                                                           <  2:1302344/132‑1 (MQ=255)          aGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATgc                                                                                                                           >  1:1302344/1‑132 (MQ=255)            aCAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATAc                                                                                                                 >  2:970659/1‑140 (MQ=255)                cGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCACCCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGgc                                                                                                             <  1:1176088/140‑1 (MQ=255)                cGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGgc                                                                                                             <  2:78937/140‑1 (MQ=255)                cGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCCGAGCTTCCTCAACTTCATGCAGCAAAATACCGgc                                                                                                             <  2:908712/140‑1 (MQ=255)                 gACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGgcg                                                                                                            <  2:1043003/140‑1 (MQ=255)                  acaacaCTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGgcgc                                                                                                           <  1:1099988/140‑1 (MQ=255)                   caacaCTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCt                                                                                                          <  2:1097217/140‑1 (MQ=255)                     acacTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTaa                                                                                                        <  2:443952/140‑1 (MQ=255)                     acacTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTaa                                                                                                        >  2:1090976/1‑140 (MQ=255)                     acacTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTaa                                                                                                        <  1:749102/140‑1 (MQ=255)                          ggAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAata                                                                                                   >  1:1165752/1‑140 (MQ=255)                          ggAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAata                                                                                                   <  1:560717/140‑1 (MQ=255)                              tttAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATtgt                                                                                               <  1:990841/140‑1 (MQ=255)                                tAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTgg                                                                                             >  1:1032946/1‑140 (MQ=255)                                 aaTGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGc                                                                                            <  1:1079798/140‑1 (MQ=255)                                   tGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCaa                                                                                          <  1:966898/140‑1 (MQ=255)                                     cTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAacc                                                                                        >  2:14919/1‑140 (MQ=255)                                             acaTTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGaa                                                                                <  1:1090976/140‑1 (MQ=255)                                                  aGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCt                                                                           >  2:399134/1‑140 (MQ=255)                                                   gTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTa                                                                          <  1:968928/140‑1 (MQ=255)                                                    tGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTAc                                                                         >  1:334651/1‑140 (MQ=255)                                                    tGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTAc                                                                         >  1:638625/1‑140 (MQ=255)                                                         ggTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAAcc                                                                    >  2:1197958/1‑140 (MQ=255)                                                         ggTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAAcc                                                                    >  1:263578/1‑140 (MQ=255)                                                         ggTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAAcc                                                                    >  1:112839/1‑140 (MQ=255)                                                          gTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCg                                                                   >  2:748748/1‑140 (MQ=255)                                                           tGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCgg                                                                  >  2:533955/1‑140 (MQ=255)                                                           tGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCgg                                                                  <  2:638625/140‑1 (MQ=255)                                                           tGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCgg                                                                  >  2:1337472/1‑140 (MQ=255)                                                           tGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCgg                                                                  >  1:809746/1‑140 (MQ=255)                                                           tGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCgg                                                                  >  1:1060179/1‑140 (MQ=255)                                                            gCGATGGCGAATAATATCGCAACCGGGGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCggg                                                                 <  1:520840/140‑1 (MQ=255)                                                             cGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGc                                                                >  1:43907/1‑140 (MQ=255)                                                             cGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGc                                                                <  1:14919/140‑1 (MQ=255)                                                             cGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGc                                                                <  1:1337472/140‑1 (MQ=255)                                                             cGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGc                                                                >  1:552907/1‑140 (MQ=255)                                                             cGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGc                                                                <  2:576846/140‑1 (MQ=255)                                                             cGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGACAACCACCCAGAACGGCTACAAACCGGGc                                                                <  1:901016/140‑1 (MQ=255)                                                              gATGGCGAATAATATCGCACCCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCg                                                               <  2:1109569/140‑1 (MQ=255)                                                               agggCAAATAATATCGCAACCGGCGAAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGa                                                              <  2:809746/138‑1 (MQ=255)                                                                tGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGAt                                                             >  1:47156/1‑140 (MQ=255)                                                                tGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGAt                                                             >  1:1311106/1‑140 (MQ=255)                                                                tGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGAt                                                             <  1:954181/140‑1 (MQ=255)                                                                   cGAATAATAACCCAGCCGGAACAATTACCGGTGCAAAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTg                                                          <  2:263578/140‑1 (MQ=255)                                                                     aataatATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGt                                                        <  1:1295695/140‑1 (MQ=255)                                                                       taataTCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGa                                                      <  2:552907/140‑1 (MQ=255)                                                                            tCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTat                                                 >  1:1146773/1‑140 (MQ=255)                                                                            tCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTat                                                 <  2:1032946/140‑1 (MQ=255)                                                                             cGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTatc                                                >  1:814646/1‑140 (MQ=255)                                                                               cAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTatcaa                                              >  2:864172/1‑140 (MQ=255)                                                                               cAACCGGCGAAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTatcaa                                              <  2:866903/140‑1 (MQ=255)                                                                                aaCCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTatcaaa                                             >  2:1281212/1‑140 (MQ=255)                                                                                  ccGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTatcaaatc                                           >  1:955364/1‑140 (MQ=255)                                                                                        cAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAatgat                                     <  1:970659/140‑1 (MQ=255)                                                                                            tAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGacg                                 <  2:47156/140‑1 (MQ=255)                                                                                            tAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGacg                                 <  2:1311106/140‑1 (MQ=255)                                                                                               cGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGacggta                              <  2:334651/140‑1 (MQ=255)                                                                                               cGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGacggta                              <  2:549774/140‑1 (MQ=255)                                                                                                ggTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGacggtac                             >  1:1332224/1‑140 (MQ=255)                                                                                                ggTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGATGAGTTATCAAATCAATGATGacggtac                             >  1:1082395/1‑140 (MQ=255)                                                                                                  tGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGacggtacgg                           >  2:581618/1‑140 (MQ=255)                                                                                                    cAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGtt                         >  2:259233/1‑140 (MQ=255)                                                                                                       aaCCCGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTc                      >  1:182814/1‑140 (MQ=255)                                                                                                          ccGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGc                   <  1:810266/140‑1 (MQ=255)                                                                                                          ccGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGc                   <  1:259233/140‑1 (MQ=255)                                                                                                          ccGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGc                   <  2:94774/140‑1 (MQ=255)                                                                                                          ccGCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGATGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGc                   <  2:1082395/140‑1 (MQ=255)                                                                                                            gCCACGTTTAGTCTGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGCaa                 >  2:1020752/1‑140 (MQ=255)                                                                                                                         tGAGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGCAACTATTCCAACGaa    <  2:182814/140‑1 (MQ=255)                                                                                                                           aGCTTCCTCAACTTCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTTGGCAACTATTCCAACGAACa  >  1:1137347/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                               GCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGCAACTATTCCAACGAACA  >  NC_000913/1133232‑1133493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑