Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 77,407 A→G noncoding (41/227 nt)
H7R (CAC→CGC) 		 sgrS →
sgrT →		 small regulatory RNA SgrS
putative inhibitor of the PtsG glucose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091377,4070AG100.0% 309.4 / NA 86noncoding (41/227 nt)
H7R (CAC→CGC) 		sgrS
sgrT		small regulatory RNA SgrS
putative inhibitor of the PtsG glucose transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/43);  total (43/43)

GTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGA  >  NC_000913/77278‑77539
                                                                                                                                 |                                                                                                                                    
gttgCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTAcc                                                                                                                            >  1:448400/1‑140 (MQ=255)
    cAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGa                                                                                                                        <  2:674819/140‑1 (MQ=255)
      gACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACa                                                                                                                      >  2:282646/1‑140 (MQ=255)
        cGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGc                                                                                                                    >  1:187952/1‑140 (MQ=255)
        cGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGc                                                                                                                    >  1:1255855/1‑140 (MQ=255)
         gAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCg                                                                                                                   <  1:317999/140‑1 (MQ=255)
         gAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCg                                                                                                                   <  1:1181197/140‑1 (MQ=255)
           gCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAA‑‑GGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGt                                                                                                               >  2:1083712/1‑140 (MQ=255)
            cAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAg                                                                                                                >  2:649406/1‑140 (MQ=255)
                  gCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGc                                                                                                          <  1:282646/140‑1 (MQ=255)
                   cATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGaa                                                                                                                 <  2:494289/132‑1 (MQ=255)
                   cATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGaa                                                                                                                 >  1:494289/1‑132 (MQ=255)
                   cATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCt                                                                                                         <  2:1233859/140‑1 (MQ=255)
                     tAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTgg                                                                                                       >  1:531396/1‑140 (MQ=255)
                        aaGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTg                                                                                                    <  1:649406/140‑1 (MQ=255)
                             gAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTg                                                                                               >  1:1127568/1‑140 (MQ=255)
                                  ccTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTaa                                                                                          >  1:388361/1‑140 (MQ=255)
                                      tgCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGt                                                                                      >  2:726850/1‑140 (MQ=255)
                                       gCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTc                                                                                     <  2:448400/140‑1 (MQ=255)
                                          aaaGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCca                                                                                  <  2:444503/140‑1 (MQ=255)
                                          aaaGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCca                                                                                  <  2:478740/140‑1 (MQ=255)
                                           aaGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCcac                                                                                 <  2:388361/140‑1 (MQ=255)
                                           aaGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCcac                                                                                 >  1:463286/1‑140 (MQ=255)
                                            aGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCcaca                                                                                <  2:346765/140‑1 (MQ=255)
                                             gACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACaa                                                                               >  1:648128/1‑140 (MQ=255)
                                                aGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGa                                                                            >  1:46093/1‑140 (MQ=255)
                                                  cAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGAtt                                                                          >  2:189953/1‑140 (MQ=255)
                                                      tttattttCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAAcc                                                                      <  2:531396/140‑1 (MQ=255)
                                                              cccTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGa                                                              <  1:1117394/140‑1 (MQ=255)
                                                                cTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAg                                                            <  2:187952/140‑1 (MQ=255)
                                                                 tataTTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTGGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAgg                                                           >  2:1264764/1‑140 (MQ=255)
                                                                  atatTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGa                                                          <  1:859165/140‑1 (MQ=255)
                                                                   tatTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGAc                                                         <  1:631901/140‑1 (MQ=255)
                                                                    atTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAAc                                                                       >  2:73436/1‑125 (MQ=255)
                                                                    atTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAAc                                                                       <  1:73436/125‑1 (MQ=255)
                                                                     ttAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTg                                                       <  2:463286/140‑1 (MQ=255)
                                                                      tAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGa                                                      <  1:1264764/140‑1 (MQ=255)
                                                                      tAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGa                                                      <  2:1127568/140‑1 (MQ=255)
                                                                        aGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATg                                                    >  1:240617/1‑140 (MQ=255)
                                                                         gTCAATAATTCCTAACGATGATGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTg                                                       >  2:965555/1‑136 (MQ=255)
                                                                         gTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTg                                                       <  1:965555/136‑1 (MQ=255)
                                                                             ataatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGa                                                              >  2:884463/1‑125 (MQ=255)
                                                                             ataatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGa                                                              <  1:884463/125‑1 (MQ=255)
                                                                             ataatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAg                                                 >  1:72118/1‑138 (MQ=255)
                                                                             ataatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAg                                                 <  2:72118/138‑1 (MQ=255)
                                                                             ataatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTg                                               >  2:1346634/1‑140 (MQ=255)
                                                                             ataatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTg                                               >  1:565922/1‑140 (MQ=255)
                                                                             ataatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTg                                               >  2:1059059/1‑140 (MQ=255)
                                                                             ataatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTg                                               >  1:369033/1‑140 (MQ=255)
                                                                             ataatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTg                                               >  1:1363725/1‑140 (MQ=255)
                                                                             ataatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTg                                               >  2:893431/1‑140 (MQ=255)
                                                                              taatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGt                                                >  1:861265/1‑138 (MQ=255)
                                                                              taatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGt                                                >  1:252066/1‑138 (MQ=255)
                                                                              taatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGt                                                <  2:861265/138‑1 (MQ=255)
                                                                              taatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGt                                                <  2:252066/138‑1 (MQ=255)
                                                                               aatTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTggg                                             <  1:339827/140‑1 (MQ=255)
                                                                                    cTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGAc                                        >  2:675233/1‑140 (MQ=255)
                                                                                      aaCGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCg                                      <  1:675233/140‑1 (MQ=255)
                                                                                      aaCGATGAAGCAA‑‑GGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATACAGTGGGATGACCGCa                                    <  1:1083712/140‑1 (MQ=255)
                                                                                       aCGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGc                                     <  1:726850/140‑1 (MQ=255)
                                                                                           tGAAGCAAGGGGGTGCCCCATGCGTCAGTTCTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAAtt                                 >  1:1333817/1‑140 (MQ=255)
                                                                                            gAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGc                                     >  2:724676/1‑135 (MQ=255)
                                                                                            gAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGc                                     <  1:724676/135‑1 (MQ=255)
                                                                                             aaGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCt                               <  1:1059059/140‑1 (MQ=255)
                                                                                              aGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTg                              >  1:1152340/1‑140 (MQ=255)
                                                                                                    ggggTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGtt                        <  2:419143/140‑1 (MQ=255)
                                                                                                      ggTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGa                      <  1:1346634/140‑1 (MQ=255)
                                                                                                      ggTGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGa                      <  2:648128/140‑1 (MQ=255)
                                                                                                        tGCCCCATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACt                    >  2:1123972/1‑140 (MQ=255)
                                                                                                          ccccATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACt                    <  2:493608/138‑1 (MQ=255)
                                                                                                          ccccATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACt                    >  1:493608/1‑138 (MQ=255)
                                                                                                          ccccATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTg                  >  1:892344/1‑140 (MQ=255)
                                                                                                          ccccATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTg                  <  2:1363725/140‑1 (MQ=255)
                                                                                                           cccATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGc                 >  1:1174021/1‑140 (MQ=255)
                                                                                                            ccATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGGCTTGcc                <  2:565922/140‑1 (MQ=255)
                                                                                                             cATGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCt               >  2:115975/1‑140 (MQ=255)
                                                                                                              aTGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTg              >  2:1273885/1‑140 (MQ=255)
                                                                                                               tGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGcc                >  1:323623/1‑137 (MQ=255)
                                                                                                               tGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGcc                <  2:323623/137‑1 (MQ=255)
                                                                                                               tGCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGc             >  1:863388/1‑140 (MQ=255)
                                                                                                                gCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGca            <  1:189953/140‑1 (MQ=255)
                                                                                                                gCGTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGca            <  2:369033/140‑1 (MQ=255)
                                                                                                                  gTCAGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGcatc          <  2:1333817/140‑1 (MQ=255)
                                                                                                                     aGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCATTTCTGAAAGTTGACTTGCCTGCATCAtg       >  2:579432/1‑140 (MQ=255)
                                                                                                                     aGTTTTATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCAtg       >  1:398577/1‑140 (MQ=255)
                                                                                                                          tATCAGCGCTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGa  <  1:893431/140‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                    
GTTGCAGACGAGCAGATGGCATAAAAGGGGAACTCCTGTGCAAAAGACAGCAATTTTATTTTCCCTATATTAAGTCAATAATTCCTAACGATGAAGCAAGGGGGTGCCCCATGCGTCAGTTTTATCAGCACTATTTTACCGCGACAGCGAAGTTGTGCTGGTTGCGTTGGTTAAGCGTCCCACAACGATTAACCATGCTTGAAGGACTGATGCAGTGGGATGACCGCAATTCTGAAAGTTGACTTGCCTGCATCATGTGTGA  >  NC_000913/77278‑77539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑