Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,385,729	A→G	G73G (GGA→GGG)	ycjF →	conserved inner membrane protein YcjF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,385,729	0	A	G	100.0%	183.0 / NA	52	G73G (GGA→GGG)	ycjF	conserved inner membrane protein YcjF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/32);  total (20/32)

CCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGGCGCTGGCGTAGGTTC  >  NC_000913/1385593‑1385863                                                                                                                                         |                                                                                                                                       ccTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGgggggg                                                                                                                                     <  2:1342927/140‑1 (MQ=255)    ttGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGggctg                                                                                                                                  >  2:879609/1‑140 (MQ=255)     tGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGggctgg                                                                                                                                 >  1:2364/1‑140 (MQ=255)       aCGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGggctggct                                                                                                                               >  1:376082/1‑140 (MQ=255)       aCGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGggctggct                                                                                                                               <  2:1056173/140‑1 (MQ=255)          aaaaTCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGggctggct                                                                                                                               <  2:761783/137‑1 (MQ=255)          aaaaTCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGggctggct                                                                                                                               >  1:761783/1‑137 (MQ=255)           aaaTCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGt                                                                                                                           >  2:477663/1‑140 (MQ=255)                 ggCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGgcg                                                                                                                     >  2:187120/1‑140 (MQ=255)                 ggCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCACAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGgcg                                                                                                                     <  2:1035260/140‑1 (MQ=255)                  gcgcAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATCGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGgcgc                                                                                                                    <  2:946034/140‑1 (MQ=255)                            ttGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGTAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTc                                                                                                          <  1:256950/140‑1 (MQ=255)                                cccGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGc                                                                                                      <  1:879609/140‑1 (MQ=255)                                cccGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGc                                                                                                      <  1:635271/140‑1 (MQ=255)                                cccGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGc                                                                                                      <  1:519891/140‑1 (MQ=255)                                cccGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGc                                                                                                      <  1:477663/140‑1 (MQ=255)                                 ccGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCa                                                                                                     <  2:376082/140‑1 (MQ=255)                                  cGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAg                                                                                                    <  2:2912/140‑1 (MQ=255)                                  cGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAg                                                                                                    >  2:694831/1‑140 (MQ=255)                                     ccACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGgt                                                                                                 <  1:91531/140‑1 (MQ=255)                                        cGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTa                                                                                              <  2:527586/140‑1 (MQ=255)                                         gCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTAc                                                                                             <  1:694831/140‑1 (MQ=255)                                              acgaAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTgg                                                                                        >  2:95855/1‑140 (MQ=255)                                              acgaAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTgg                                                                                        <  1:1279097/140‑1 (MQ=255)                                              acgaAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTgg                                                                                        >  1:216105/1‑140 (MQ=255)                                               cgaAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGa                                                                                       >  1:800974/1‑140 (MQ=255)                                               cgaAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGa                                                                                       <  1:1090908/140‑1 (MQ=255)                                                    cgcAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACaatg                                                                                  >  2:891873/1‑140 (MQ=255)                                                     gcAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACaatga                                                                                 <  2:1194852/140‑1 (MQ=255)                                                              agagGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGc                                                                        <  1:95855/140‑1 (MQ=255)                                                              agagGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGc                                                                        <  2:517109/140‑1 (MQ=255)                                                                    gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAAc                                                                    >  2:556139/1‑138 (MQ=255)                                                                    gCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAAc                                                                    <  1:556139/138‑1 (MQ=255)                                                                       aGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAgg                                                               >  1:715893/1‑140 (MQ=255)                                                                             aGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTggg                                                         <  2:715893/140‑1 (MQ=255)                                                                                ggTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTgg                                                      <  2:937116/140‑1 (MQ=255)                                                                                ggTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTgg                                                      <  2:216105/140‑1 (MQ=255)                                                                                  tAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAAc                                                                    >  1:881433/1‑124 (MQ=255)                                                                                  tAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAAc                                                                    <  2:881433/124‑1 (MQ=255)                                                                                            aGCGTTACGTCCGAAACGCAGGCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACGATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGAt                                          >  2:556607/1‑140 (MQ=255)                                                                                             gCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGAtg                                         <  2:2364/140‑1 (MQ=255)                                                                                                      ccGAAACGCAGCCTGTGGCGCAATTTGGTGATGGGGGGGGTGGCTCTCTTTGGCGCAAGCGCTGCCGGGCAGGGCGTACAGTGGACAATGATTGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGCGGCTGTGCCGCTgg                                >  2:611470/1‑140 (MQ=255)                                                                                                        gAAACGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTgggg                              >  2:180601/1‑140 (MQ=255)                                                                                                          aaCGCAGCCTGTGGCGCAAAATGGTGATGGGGGGACTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAGCCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCa                            <  1:611470/140‑1 (MQ=255)                                                                                                            cGCAGCCTGTGGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCAtt                          <  1:187120/140‑1 (MQ=255)                                                                                                                   tgtgGCGCAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATc                   <  2:800974/140‑1 (MQ=255)                                                                                                                       gcgcAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGgcg               >  2:157238/1‑140 (MQ=255)                                                                                                                          cAAAATGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGGCGCTg            <  1:157238/140‑1 (MQ=255)                                                                                                                           aaaaTGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGgcg               >  2:480334/1‑136 (MQ=255)                                                                                                                           aaaaTGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGgcg               <  1:480334/136‑1 (MQ=255)                                                                                                                               tGGTGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGGCGCTGGCGTa       <  1:335193/140‑1 (MQ=255)                                                                                                                                  tGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACGATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGGCGCTGGCGTAGGt    <  1:556607/140‑1 (MQ=255)                                                                                                                                  tGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGGCGCTGGCGTAGGt    >  1:95095/1‑140 (MQ=255)                                                                                                                                  tGATGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGGCGCTGGCGTAGGt    >  1:62297/1‑140 (MQ=255)                                                                                                                                    gtGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGGCGCTGGCGTAGGTTc  <  2:162823/139‑1 (MQ=255)                                                                                                                                    aTGGGGGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGGCGCTGGCGTAGGTTc  <  1:180601/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CCTTTGACGAAAATCAGGCGCAAAATTTTGCCCCGGCCACGCTCGACGAAGCGCAGGAAGAAGAGGGGCAAGTCGAAGCGGTAATGGACGCAGCGTTACGTCCGAAACGCAGCCTGTGGCGCAAAATGGTGATGGGAGGGCTGGCTCTGTTTGGCGCAAGCGTTGTCGGGCAGGGTGTACAGTGGACAATGAATGCCTGGCAAACCCAGGACTGGGTGGCGCTGGGTGGATGTGCCGCTGGGGCATTGATTATCGGCGCTGGCGTAGGTTC  >  NC_000913/1385593‑1385863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑