Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,569,408	C→T	G361D (GGC→GAC)	gadC ←	L‑glutamate:4‑aminobutyrate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,569,408	0	C	T	100.0%	340.1 / NA	96	G361D (GGC→GAC)	gadC	L‑glutamate:4‑aminobutyrate antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/46);  total (50/46)

AGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACAT  >  NC_000913/1569272‑1569544                                                                                                                                         |                                                                                                                                         aGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCAc                                                                                                                                       <  2:1359209/140‑1 (MQ=255) aGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCAc                                                                                                                                       <  2:260549/140‑1 (MQ=255) aGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCAc                                                                                                                                       <  2:587498/140‑1 (MQ=255)  gggATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCAcc                                                                                                                                      >  1:761317/1‑140 (MQ=255)   ggATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCg                                                                                                                                     >  1:882184/1‑140 (MQ=255)    gATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCAc                                                                                                                                       <  1:624493/137‑1 (MQ=255)    gATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCAc                                                                                                                                       >  2:624493/1‑137 (MQ=255)     atatTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGt                                                                                                                                   <  1:1099188/140‑1 (MQ=255)     atatTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGt                                                                                                                                   <  2:59947/140‑1 (MQ=255)      tatTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTa                                                                                                                                  >  2:993834/1‑140 (MQ=255)         tAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTg                                                                                                                               <  2:761317/140‑1 (MQ=255)          aaaTGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTgg                                                                                                                              <  1:993834/140‑1 (MQ=255)             tgtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCAc                                                                                                                                       >  2:289513/1‑128 (MQ=255)             tgtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCAc                                                                                                                                       >  2:130919/1‑128 (MQ=255)             tgtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCAc                                                                                                                                       <  1:289513/128‑1 (MQ=255)             tgtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCAc                                                                                                                                       <  1:130919/128‑1 (MQ=255)              gtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAg                                                                                                                          >  1:1006068/1‑140 (MQ=255)              gtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAg                                                                                                                          >  1:609139/1‑140 (MQ=255)              gtgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAg                                                                                                                          >  2:164497/1‑140 (MQ=255)               tgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAgg                                                                                                                         >  2:594092/1‑140 (MQ=255)               tgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAgg                                                                                                                         >  1:1025845/1‑140 (MQ=255)               tgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAgg                                                                                                                         >  2:71297/1‑140 (MQ=255)               tgCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAgg                                                                                                                         >  2:571504/1‑140 (MQ=255)                 cGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTAt                                                                                                                                 >  2:1192374/1‑130 (MQ=255)                 cGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTAt                                                                                                                                 <  1:1192374/130‑1 (MQ=255)                 cGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGgat                                                                                                                       >  2:29571/1‑140 (MQ=255)                  gTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGgatg                                                                                                                      <  2:1006068/140‑1 (MQ=255)                       aaGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCaa                                                                                                                 <  1:29571/140‑1 (MQ=255)                       aaGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCaa                                                                                                                 <  2:1076924/140‑1 (MQ=255)                            aGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGa                                                                                                            <  2:882184/140‑1 (MQ=255)                              gATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGa                                                                                                            >  2:110956/1‑138 (MQ=255)                              gATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGa                                                                                                            <  1:110956/138‑1 (MQ=255)                              gATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATa                                                                                                          >  1:1210611/1‑140 (MQ=255)                                       gAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATc                                                                                                 <  2:609139/140‑1 (MQ=255)                                        aaCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCa                                                                                                >  2:153430/1‑140 (MQ=255)                                        aaCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCa                                                                                                >  1:310089/1‑140 (MQ=255)                                        aaCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCa                                                                                                >  1:1066024/1‑140 (MQ=255)                                              cacaATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCt                                                                                          <  2:558210/140‑1 (MQ=255)                                              cacaATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCt                                                                                          >  1:99761/1‑140 (MQ=255)                                                 aaTGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCg                                                                                       >  1:241595/1‑140 (MQ=255)                                                   tGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGaa                                                                                     <  2:104487/140‑1 (MQ=255)                                                        ccAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGAc                                                                                <  1:704133/140‑1 (MQ=255)                                                        ccAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGAc                                                                                >  1:883166/1‑140 (MQ=255)                                                        ccAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGAc                                                                                >  1:272872/1‑140 (MQ=255)                                                         cAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGAcc                                                                               <  2:241595/140‑1 (MQ=255)                                                         cAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGAcc                                                                               <  2:99761/140‑1 (MQ=255)                                                           aTAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAg                                                                             >  1:982768/1‑140 (MQ=255)                                                           aTAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAg                                                                             >  2:942770/1‑140 (MQ=255)                                                             aaaCAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCg                                                                           >  1:345916/1‑140 (MQ=255)                                                              aaCAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGt                                                                          <  1:164497/140‑1 (MQ=255)                                                                  gCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTAcc                                                                      >  1:1062113/1‑140 (MQ=255)                                                                   cATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGAcc                                                                               >  2:135986/1‑130 (MQ=255)                                                                   cATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGAcc                                                                               <  1:135986/130‑1 (MQ=255)                                                                   cATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCg                                                                     >  1:704583/1‑140 (MQ=255)                                                                     tGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCgg                                                                    >  2:1264424/1‑139 (MQ=255)                                                                     tGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCgg                                                                    <  1:1264424/139‑1 (MQ=255)                                                                      gAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTa                                                                  >  2:138944/1‑140 (MQ=255)                                                                      gAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTa                                                                  <  1:153430/140‑1 (MQ=255)                                                                      gAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTa                                                                  <  1:228857/140‑1 (MQ=255)                                                                                 acaGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTg                                                       >  1:328302/1‑140 (MQ=255)                                                                                 acaGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTg                                                       >  1:723048/1‑140 (MQ=255)                                                                                  caGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGt                                                      <  2:704583/140‑1 (MQ=255)                                                                                       aaaTCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCAtt                                                 >  2:616523/1‑140 (MQ=255)                                                                                              caccGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGa                                          <  2:982768/140‑1 (MQ=255)                                                                                               accGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGaa                                         >  1:1229169/1‑140 (MQ=255)                                                                                               accGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGaa                                         >  1:582216/1‑140 (MQ=255)                                                                                               accGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGaa                                         >  2:890772/1‑140 (MQ=255)                                                                                                 cGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATg                                       >  1:300853/1‑140 (MQ=255)                                                                                                    cAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGa                                          >  2:1023193/1‑134 (MQ=255)                                                                                                    cAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGa                                          <  1:1023193/134‑1 (MQ=255)                                                                                                          cAGTGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGgca                              <  1:571504/140‑1 (MQ=255)                                                                                                             tGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGgcagca                           >  2:1305815/1‑140 (MQ=255)                                                                                                             tGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGgcagca                           >  2:1097532/1‑140 (MQ=255)                                                                                                             tGCGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGgcagca                           >  1:736856/1‑140 (MQ=255)                                                                                                               cGATCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAgg                         <  2:310089/140‑1 (MQ=255)                                                                                                                 aTCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGtt                       >  1:288683/1‑140 (MQ=255)                                                                                                                 aTCAGGAAGGACATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGtt                       >  1:232707/1‑140 (MQ=255)                                                                                                                       aaggaCATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTg                 <  2:232707/140‑1 (MQ=255)                                                                                                                       aaggaCATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTg                 <  2:328302/140‑1 (MQ=255)                                                                                                                       aaggaCATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTg                 <  1:594092/140‑1 (MQ=255)                                                                                                                       aaggaCATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTg                 <  1:1097532/140‑1 (MQ=255)                                                                                                                         ggaCATGTTGTTACCGTCAGCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTgcg               <  2:1025845/140‑1 (MQ=255)                                                                                                                         ggaCATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTgcg               <  2:288683/140‑1 (MQ=255)                                                                                                                         ggaCATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTgcg               <  1:1272591/140‑1 (MQ=255)                                                                                                                         ggaCATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTgcg               <  2:345916/140‑1 (MQ=255)                                                                                                                         ggaCATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTgcg               <  1:794633/140‑1 (MQ=255)                                                                                                                           aCATGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCt             <  1:890772/140‑1 (MQ=255)                                                                                                                             aTGTTGTTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGt           <  1:138944/140‑1 (MQ=255)                                                                                                                              tgttgtTACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGtt          >  1:1212387/1‑140 (MQ=255)                                                                                                                                ttgttACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTg                 >  2:74357/1‑131 (MQ=255)                                                                                                                                ttgttACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTg                 <  1:74357/131‑1 (MQ=255)                                                                                                                                ttgttACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTtac        >  2:2339/1‑140 (MQ=255)                                                                                                                                ttgttACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTtac        >  2:108002/1‑140 (MQ=255)                                                                                                                                    tACCGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTtacatac    >  2:1331842/1‑140 (MQ=255)                                                                                                                                      ccGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTtacataca   >  2:882555/1‑139 (MQ=255)                                                                                                                                      ccGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTtacataca   <  1:882555/139‑1 (MQ=255)                                                                                                                                      ccGTCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTacatacat  >  2:776264/1‑140 (MQ=255)                                                                                                                                      ccGTCACCGGTATTGGTCAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTacatacat  <  2:1212387/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGATAGACGTAATCACCAGCTGCGAAATGACCAGCGTTACCGGTACGCCATTTTTGTTCATTTTAGCGAATGCCGCTGGCAGCAGGTTTTTCTGCGCTGTTACATACAT  >  NC_000913/1569272‑1569544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑