Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,743,004	T→C	D80G (GAT→GGT)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,743,004	0	T	C	100.0%	225.3 / NA	64	D80G (GAT→GGT)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/30);  total (34/30)

GACTTTAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCC  >  NC_000913/1742868‑1743140                                                                                                                                         |                                                                                                                                         gACTTTAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAAcccc                                                                                                                                       <  2:888568/140‑1 (MQ=255)  aCTTTAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACcccc                                                                                                                                      <  2:1238527/140‑1 (MQ=255)      tAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACt                                                                                                                                  >  2:805155/1‑140 (MQ=255)      tAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACt                                                                                                                                  >  2:450655/1‑140 (MQ=255)      tAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACt                                                                                                                                  >  2:190084/1‑140 (MQ=255)              aTCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATc                                                                                                                          >  1:997932/1‑140 (MQ=255)                 tGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGcc                                                                                                                       <  1:450655/140‑1 (MQ=255)                 tGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGcc                                                                                                                       <  1:19468/140‑1 (MQ=255)                 tGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGcc                                                                                                                       <  1:190084/140‑1 (MQ=255)                  ggCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCa                                                                                                                      >  1:1187398/1‑140 (MQ=255)                      attattTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGAt                                                                                                                  <  1:805155/140‑1 (MQ=255)                                     tAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACg                                                                                                   <  2:997932/140‑1 (MQ=255)                                      aaTATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGt                                                                                                  >  1:118230/1‑140 (MQ=255)                                             tCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGtttctttc                                                                                           >  2:422594/1‑140 (MQ=255)                                               gCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTtcat                                                                                         <  1:150456/140‑1 (MQ=255)                                                  aCTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAg                                                                                      >  2:1182589/1‑140 (MQ=255)                                                    ttCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACg                                                                                                   <  2:50089/125‑1 (MQ=255)                                                    ttCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACg                                                                                                   >  1:50089/1‑125 (MQ=255)                                                       agcagTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCaa                                                                                 <  1:279448/140‑1 (MQ=255)                                                       agcagTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCaa                                                                                 <  2:1187398/140‑1 (MQ=255)                                                        gcagTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCaaa                                                                                <  1:86566/140‑1 (MQ=255)                                                              gTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGAc                                                                          >  1:309914/1‑140 (MQ=255)                                                                   aataTGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCACCGTTTATCCAACCCCCCTCTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGt                                                                     <  2:309914/140‑1 (MQ=255)                                                                        gACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCg                                                                >  2:1194198/1‑140 (MQ=255)                                                                        gACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCg                                                                >  1:620805/1‑140 (MQ=255)                                                                        gACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCg                                                                >  1:411534/1‑140 (MQ=255)                                                                        gACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCg                                                                >  1:801884/1‑140 (MQ=255)                                                                        gACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCg                                                                >  1:993464/1‑140 (MQ=255)                                                                         aCCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGt                                                               >  1:1002772/1‑140 (MQ=255)                                                                          ccTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGtt                                                              >  2:71298/1‑140 (MQ=255)                                                                               cATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAAttt                                                         >  2:315457/1‑140 (MQ=255)                                                                                aTTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTc                                                        >  1:176789/1‑140 (MQ=255)                                                                                   aaGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGt                                                     <  2:1002772/140‑1 (MQ=255)                                                                                     gtgtCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCa                                                   >  1:168188/1‑140 (MQ=255)                                                                                      tgtCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCAc                                                  >  1:452861/1‑140 (MQ=255)                                                                                       gtCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACg                                                 <  2:452861/140‑1 (MQ=255)                                                                                        tCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGcc                                                                 <  2:1360070/123‑1 (MQ=255)                                                                                        tCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGcc                                                                 >  1:1360070/1‑123 (MQ=255)                                                                                        tCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAAttt                                                         >  2:228584/1‑131 (MQ=255)                                                                                        tCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAAttt                                                         <  1:228584/131‑1 (MQ=255)                                                                                        tCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGt                                                     >  1:822058/1‑135 (MQ=255)                                                                                        tCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGt                                                     <  2:822058/135‑1 (MQ=255)                                                                                        tCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACgg                                                >  1:529381/1‑140 (MQ=255)                                                                                        tCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACgg                                                >  2:915037/1‑140 (MQ=255)                                                                                        tCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACgg                                                >  1:481526/1‑140 (MQ=255)                                                                                           gCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCa                                             <  2:411534/140‑1 (MQ=255)                                                                                                tttCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGgcag                                        <  2:993464/140‑1 (MQ=255)                                                                                                tttCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGgcag                                        <  2:801884/140‑1 (MQ=255)                                                                                                  tcatcaCTGAATTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGgcagca                                      <  2:176789/140‑1 (MQ=255)                                                                                                           aaTTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATg                             <  1:1194198/140‑1 (MQ=255)                                                                                                           aaTTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATg                             >  1:826055/1‑140 (MQ=255)                                                                                                            aTTTCGCCGCACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATgc                            <  1:71298/140‑1 (MQ=255)                                                                                                                   cgcACGCTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACgg                     <  2:100161/140‑1 (MQ=255)                                                                                                                         cTCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCAc               >  1:314208/1‑140 (MQ=255)                                                                                                                          tCAACGTTTACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCAcc              >  1:157813/1‑140 (MQ=255)                                                                                                                                  tACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCAcc              >  1:904319/1‑132 (MQ=255)                                                                                                                                  tACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCAcc              <  2:904319/132‑1 (MQ=255)                                                                                                                                  tACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCa      >  2:63347/1‑140 (MQ=255)                                                                                                                                  tACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCa      >  2:1034788/1‑140 (MQ=255)                                                                                                                                  tACCCAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCa      <  2:168188/140‑1 (MQ=255)                                                                                                                                    cccAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAgg    <  1:1182589/140‑1 (MQ=255)                                                                                                                                    cccAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAgg    <  1:63347/140‑1 (MQ=255)                                                                                                                                    cccAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAgg    <  2:826055/140‑1 (MQ=255)                                                                                                                                    cccAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAgg    >  1:1057958/1‑140 (MQ=255)                                                                                                                                     ccAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGc   <  2:157813/140‑1 (MQ=255)                                                                                                                                      cAACCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGACAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGcc  <  2:1242468/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GACTTTAAACCAGATCTGGCGATTATTTTCTGAGGTTAATATTTTCGCCACTTCAGCAGTGGTCATAATATGACCTGACATTAAGTGTCCGCCAATTTCATCACTGAATTTCGCCGCACGCTCAACGTTTACCCAATCCCCCACTTTTAAATCGCCAAGATTGGTAATGCGTAACGTTTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCC  >  NC_000913/1742868‑1743140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑