Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,865,871	A→G	L201P (CTG→CCG)	mipA ←	scaffolding protein that interacts with murein polymerase and murein hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,865,871	0	A	G	100.0%	193.2 / NA	54	L201P (CTG→CCG)	mipA	scaffolding protein that interacts with murein polymerase and murein hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/24);  total (30/24)

TGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTC  >  NC_000913/1865734‑1866005                                                                                                                                          |                                                                                                                                       tGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTTAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGc                                                                                                                                      >  2:1100455/1‑140 (MQ=255) tGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGc                                                                                                                                      >  1:1091260/1‑140 (MQ=255)   tAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTc                                                                                                                                    <  2:795612/140‑1 (MQ=255)   tAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTc                                                                                                                                    >  1:567172/1‑140 (MQ=255)       tGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAgg                                                                                                                                >  2:323866/1‑140 (MQ=255)           cccGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAg                                                                                                                            >  2:803819/1‑140 (MQ=255)                   aaaTCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAg                                                                                                                    >  2:1180137/1‑140 (MQ=255)                    aaTCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGc                                                                                                                   <  1:603195/140‑1 (MQ=255)                     aTCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCt                                                                                                                  >  2:307857/1‑140 (MQ=255)                         ggCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCg                                                                                                              >  1:1041147/1‑140 (MQ=255)                                       ttATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGcc                                                                                                >  2:792626/1‑140 (MQ=255)                                         aTCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCAc                                                                                              >  1:303983/1‑140 (MQ=255)                                         aTCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCAc                                                                                              >  2:707517/1‑140 (MQ=255)                                          tCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACg                                                                                             >  2:389819/1‑140 (MQ=255)                                                    ggCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTg                                                                                   >  1:275554/1‑140 (MQ=255)                                                    ggCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTg                                                                                   <  2:567172/140‑1 (MQ=255)                                                         tCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGc                                                                              >  2:879927/1‑140 (MQ=255)                                                           aGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCgg                                                                            >  1:167249/1‑140 (MQ=255)                                                              aaCTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGAct                                                                         <  1:307857/140‑1 (MQ=255)                                                                   catcaGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTg                                                                    >  2:554199/1‑140 (MQ=255)                                                                        gacagacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGa                                                               <  2:167249/140‑1 (MQ=255)                                                                        gacagacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGa                                                               <  1:803819/140‑1 (MQ=255)                                                                          cagacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATa                                                             <  1:1180137/140‑1 (MQ=255)                                                                           agacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATAc                                                            >  1:307525/1‑140 (MQ=255)                                                                              cGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGcc                                                         <  1:707517/140‑1 (MQ=255)                                                                               gggTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGTTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCa                                                        <  1:382595/140‑1 (MQ=255)                                                                               gggTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCa                                                        <  1:554199/140‑1 (MQ=255)                                                                               gggTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCa                                                        <  1:879927/140‑1 (MQ=255)                                                                                ggTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCat                                                       <  2:275554/140‑1 (MQ=255)                                                                                      gCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGt                                                 <  1:751564/140‑1 (MQ=255)                                                                                       cGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTa                                                >  2:1101668/1‑140 (MQ=255)                                                                                        gTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTAt                                               <  2:307525/140‑1 (MQ=255)                                                                                         tGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATTGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTAtt                                              >  2:1186546/1‑140 (MQ=255)                                                                                           cGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCg                                            >  1:1057534/1‑140 (MQ=255)                                                                                           cGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCg                                            >  1:482917/1‑140 (MQ=255)                                                                                           cGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCg                                            >  1:310302/1‑140 (MQ=255)                                                                                              tACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTc                                         >  1:264775/1‑140 (MQ=255)                                                                                                  gTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGt                                     <  1:649279/140‑1 (MQ=255)                                                                                                  gTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGt                                     <  1:1263835/140‑1 (MQ=255)                                                                                                   tAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGtt                                    >  2:71979/1‑140 (MQ=255)                                                                                                       cacTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCg                                <  1:1101668/140‑1 (MQ=255)                                                                                                          tCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTg                             >  1:658642/1‑140 (MQ=255)                                                                                                          tCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTg                             >  2:1057731/1‑140 (MQ=255)                                                                                                               tCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGt                                     <  2:887009/127‑1 (MQ=255)                                                                                                               tCGCCGAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGt                                     >  1:887009/1‑127 (MQ=255)                                                                                                                    gAGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGca                   <  2:1057534/140‑1 (MQ=255)                                                                                                                     aGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGtt                           >  2:1223064/1‑131 (MQ=255)                                                                                                                     aGGAAGTTGTAGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGtt                           <  1:1223064/131‑1 (MQ=255)                                                                                                                               aGCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAccc        >  1:421837/1‑140 (MQ=255)                                                                                                                                gCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCg       <  2:264775/140‑1 (MQ=255)                                                                                                                                gCTGGCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCg       <  1:1057731/140‑1 (MQ=255)                                                                                                                                   ggCGCTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAg    <  2:303983/140‑1 (MQ=255)                                                                                                                                    gcgcTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGt   <  2:571317/140‑1 (MQ=255)                                                                                                                                     cgcTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTc  >  1:1079604/1‑140 (MQ=255)                                                                                                                                     cgcTCGGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTc  >  2:1210250/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTC  >  NC_000913/1865734‑1866005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑