Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,948,205	T→C	E143G (GAA→GGA)	nudB ←	dihydroneopterin triphosphate diphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,948,205	0	T	C	100.0%	234.0 / NA	66	E143G (GAA→GGA)	nudB	dihydroneopterin triphosphate diphosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/29);  total (37/29)

ATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAACCATGATT  >  NC_000913/1948069‑1948334                                                                                                                                         |                                                                                                                                  aTTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCaa                                                                                                                                >  2:724630/1‑140 (MQ=255)    ttAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCg                                                                                                                             >  2:496493/1‑140 (MQ=255)        tGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTg                                                                                                                         >  2:122066/1‑140 (MQ=255)        tGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTg                                                                                                                         >  1:352184/1‑140 (MQ=255)        tGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTg                                                                                                                         <  1:167960/140‑1 (MQ=255)             aTTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGt                                                                                                                    <  1:286805/140‑1 (MQ=255)                  aCCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTc                                                                                                               >  2:893615/1‑140 (MQ=255)                  aCCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTc                                                                                                               >  1:520585/1‑140 (MQ=255)                   ccGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGCCCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTcc                                                                                                              <  2:352184/140‑1 (MQ=255)                   ccGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTcc                                                                                                              <  2:281737/140‑1 (MQ=255)                   ccGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTcc                                                                                                              <  1:999758/140‑1 (MQ=255)                   ccGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTcc                                                                                                              <  1:68940/140‑1 (MQ=255)                     gcgcTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAg                                                                                                            <  2:820711/140‑1 (MQ=255)                       gcTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGa                                                                                                          >  2:299144/1‑140 (MQ=255)                             cATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGt                                                                                                    >  2:89819/1‑140 (MQ=255)                                  tGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAgcg                                                                                               <  2:18789/140‑1 (MQ=255)                                  tGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAgcg                                                                                               >  2:987931/1‑140 (MQ=255)                                     gcAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGcgccg                                                                                            >  1:1205216/1‑140 (MQ=255)                                     gcAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCAGTTGCTCCAGGACTTAGTGAGcgccg                                                                                            >  1:950043/1‑140 (MQ=255)                                              cGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGcagcag                                                                                   <  1:299144/140‑1 (MQ=255)                                               gATGTCTGGTGTTGGCCCTTTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAgg                                                                                  <  2:1347616/140‑1 (MQ=255)                                                  ttgtGGTGTGGCCCTTTTTACTATGCCCTGCAATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGgcgc                                                                               <  2:857683/137‑1 (MQ=255)                                                   tCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCgcag                                                                                      >  2:454551/1‑132 (MQ=255)                                                   tCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCgcag                                                                                      <  1:454551/132‑1 (MQ=255)                                                   tCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCa                                                                              >  2:172296/1‑140 (MQ=255)                                                   tCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATACCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGgcgcc                                                                              >  2:1007701/1‑139 (MQ=255)                                                       gtgtTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCaa                                                                          >  1:543010/1‑140 (MQ=255)                                                          ttGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGcc                                                                       <  1:987931/140‑1 (MQ=255)                                                             gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACt                                                                    >  1:857683/1‑140 (MQ=255)                                                             gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACt                                                                    >  2:994731/1‑140 (MQ=255)                                                             gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACt                                                                    >  2:378592/1‑140 (MQ=255)                                                             gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACt                                                                    >  1:460161/1‑140 (MQ=255)                                                             gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACt                                                                    >  2:971681/1‑140 (MQ=255)                                                             gCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACt                                                                    <  1:89819/140‑1 (MQ=255)                                                               ccttttACTATGCCCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTg                                                                  <  2:233437/137‑1 (MQ=255)                                                                  tttACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTaa                                                               <  2:520585/140‑1 (MQ=255)                                                                           aCCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATg                                                       <  2:102841/139‑1 (MQ=255)                                                                           aCCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATg                                                       >  1:102841/1‑139 (MQ=255)                                                                               gCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAg                                                  >  1:111862/1‑140 (MQ=255)                                                                               gCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAg                                                  >  1:127881/1‑140 (MQ=255)                                                                                          atCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGGTc                                       >  2:436749/1‑140 (MQ=255)                                                                                          atCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATc                                       >  1:753086/1‑140 (MQ=255)                                                                                          atCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATc                                       >  2:236917/1‑140 (MQ=255)                                                                                                     gCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTg                            <  1:236917/140‑1 (MQ=255)                                                                                                      cccTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGc                           >  2:177142/1‑140 (MQ=255)                                                                                                      cccTGCCTGTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGc                           >  2:244030/1‑140 (MQ=255)                                                                                                        ctgcctgTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCNNATGTTCAGTGAAAACGATCTGCCGCTCGTGCgg                         <  1:324716/140‑1 (MQ=255)                                                                                                        ctgcctgTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCgg                         <  2:178715/140‑1 (MQ=255)                                                                                                         tgcctgTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGa                        >  2:1120337/1‑140 (MQ=255)                                                                                                           cctgTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGGTCTGCCGCTCGTGCGGAAg                      <  1:436749/140‑1 (MQ=255)                                                                                                           cctgTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAg                      >  2:649845/1‑140 (MQ=255)                                                                                                            ctgTTCAGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCAGAAgc                     >  1:608624/1‑140 (MQ=255)                                                                                                                  aGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGa               <  1:1051902/140‑1 (MQ=255)                                                                                                                  aGGCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGa               <  1:994731/140‑1 (MQ=255)                                                                                                                   ggCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGAc              <  2:363921/140‑1 (MQ=255)                                                                                                                    gCAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACa             >  1:655917/1‑140 (MQ=255)                                                                                                                     cAGCGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAg            >  2:1030508/1‑140 (MQ=255)                                                                                                                        cGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGTAAGCGCAAGACAGAAc         >  2:303875/1‑140 (MQ=255)                                                                                                                        cGTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAAc         >  1:52802/1‑140 (MQ=255)                                                                                                                         gTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAAcc        >  2:333068/1‑140 (MQ=255)                                                                                                                         gTTAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAAcc        >  1:1335610/1‑140 (MQ=255)                                                                                                                          ttAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAACCa       <  2:460161/140‑1 (MQ=255)                                                                                                                          ttAATTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAACCa       <  2:543010/140‑1 (MQ=255)                                                                                                                            aaTTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAACCATg     <  1:724630/140‑1 (MQ=255)                                                                                                                            aaTTACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAACCATg     <  1:1120337/140‑1 (MQ=255)                                                                                                                               tACAAACTGTCCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAACCATGAtt  <  2:111862/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  ATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAAGACAGAACCATGATT  >  NC_000913/1948069‑1948334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑