Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	117,102	C→T	intergenic (‑3/+7)	hofB ← / ← ppdD	T2SSE family protein/prepilin peptidase‑dependent protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	117,102	0	C	T	100.0%	269.4 / NA	76	intergenic (‑3/+7)	hofB/ppdD	T2SSE family protein/prepilin peptidase‑dependent protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/33);  total (43/33)

GGTAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCGTTATCCCAACCCGGTGTCATGACGAC  >  NC_000913/116965‑117237                                                                                                                                          |                                                                                                                                        ggtAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCg                                                                                                                                       <  2:222385/140‑1 (MQ=255)   tAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCt                                                                                                                                     >  1:42647/1‑140 (MQ=255)   tAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCt                                                                                                                                     <  2:536577/140‑1 (MQ=255)     gCGAAATGCGATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTc                                                                                                                                    <  2:1166407/139‑1 (MQ=255)     gCGAAATGCGATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTc                                                                                                                                    >  1:1166407/1‑139 (MQ=255)     gCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTcc                                                                                                                                   <  1:224826/140‑1 (MQ=255)     gCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTcc                                                                                                                                   >  2:1089309/1‑140 (MQ=255)         aatgcaatgcGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAg                                                                                                                               <  1:298233/140‑1 (MQ=255)         aatgcaatgcGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACCCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAg                                                                                                                               <  2:978412/140‑1 (MQ=255)              aatgcGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGc                                                                                                                          <  2:318463/140‑1 (MQ=255)                tgcGTCCAGTAGCTCATGCGAAGGGGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGt                                                                                                                        >  2:208702/1‑140 (MQ=255)                   gTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACATGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGtcat                                                                                                                     <  1:963755/140‑1 (MQ=255)                     ccAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCGTCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGtcatca                                                                                                                   >  1:535283/1‑140 (MQ=255)                     ccAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGtcatca                                                                                                                   >  2:38297/1‑140 (MQ=255)                       aGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCaaa                                                                                                                 >  2:218397/1‑140 (MQ=255)                        gTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAg                                                                                                                <  2:594890/140‑1 (MQ=255)                            cTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGa                                                                                                            >  2:527115/1‑140 (MQ=255)                              cATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAg                                                                                                          >  2:1053613/1‑140 (MQ=255)                              cATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAg                                                                                                          >  1:1137773/1‑140 (MQ=255)                               aTGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGa                                                                                                         >  1:555490/1‑140 (MQ=255)                                   gAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATc                                                                                                     >  2:1325908/1‑140 (MQ=255)                                        tGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGc                                                                                                <  1:769073/140‑1 (MQ=255)                                           aTCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGGCATCTTCGCAgg                                                                                             >  2:1010352/1‑140 (MQ=255)                                           aTCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAgg                                                                                             >  1:1124090/1‑140 (MQ=255)                                           aTCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAgg                                                                                             >  1:779533/1‑140 (MQ=255)                                            tCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGc                                                                                            <  1:1053613/140‑1 (MQ=255)                                            tCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGc                                                                                            >  2:1068371/1‑140 (MQ=255)                                             cGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCt                                                                                           <  1:1077068/140‑1 (MQ=255)                                               aCTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTtg                                                                                         >  2:46737/1‑140 (MQ=255)                                               aCTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTtg                                                                                         >  1:1088492/1‑140 (MQ=255)                                                 tACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTtgct                                                                                       >  1:950510/1‑140 (MQ=255)                                                     gCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCaa                                                                                   <  2:815050/140‑1 (MQ=255)                                                       aaCATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATg                                                                                 >  2:997439/1‑140 (MQ=255)                                                         cATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATgcg                                                                               <  1:218397/140‑1 (MQ=255)                                                           tGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCt                                                                             <  1:1325908/140‑1 (MQ=255)                                                             aaCCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGt                                                                           >  1:878562/1‑140 (MQ=255)                                                                caccTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCAc                                                                        <  2:878562/140‑1 (MQ=255)                                                                 accTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACt                                                                       <  1:527115/140‑1 (MQ=255)                                                                   ctctTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACttt                                                                     >  1:975354/1‑140 (MQ=255)                                                                   ctctTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGCGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACttt                                                                     >  1:1205925/1‑140 (MQ=255)                                                                     ctTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGa                                                                   >  2:1130702/1‑140 (MQ=255)                                                                      ttCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGaa                                                                  <  1:1068371/140‑1 (MQ=255)                                                                      ttCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGaa                                                                  <  2:950510/140‑1 (MQ=255)                                                                      ttCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGaa                                                                  <  2:616317/140‑1 (MQ=255)                                                                       tCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAat                                                                 >  2:763690/1‑140 (MQ=255)                                                                         gCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAatat                                                               <  1:38297/140‑1 (MQ=255)                                                                             gCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCa                                                           >  2:1021524/1‑140 (MQ=255)                                                                                     cAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGcgcg                                                   >  1:646620/1‑140 (MQ=255)                                                                                     cAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGcgcg                                                   <  2:646620/140‑1 (MQ=255)                                                                                           tCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGc                                             >  1:620477/1‑140 (MQ=255)                                                                                            ccATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGcc                                            >  2:686781/1‑140 (MQ=255)                                                                                            ccATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGcc                                            >  2:646362/1‑140 (MQ=255)                                                                                             cATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCg                                           >  1:405396/1‑140 (MQ=255)                                                                                             cATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCg                                           >  2:728945/1‑140 (MQ=255)                                                                                             cATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGCCCAGCCg                                           >  2:858466/1‑140 (MQ=255)                                                                                              aTGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCgg                                          >  2:1295485/1‑140 (MQ=255)                                                                                                gATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTg                                        >  2:1256365/1‑140 (MQ=255)                                                                                                 aTAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGa                                       <  1:686781/140‑1 (MQ=255)                                                                                                 aTAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGa                                       <  1:646362/140‑1 (MQ=255)                                                                                                     cGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGcc                                   >  1:757783/1‑140 (MQ=255)                                                                                                       cAGACACAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGt                                 >  2:385111/1‑140 (MQ=255)                                                                                                          acacAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTg                              >  1:128242/1‑140 (MQ=255)                                                                                                          acacAGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAACCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTg                              >  1:1287120/1‑140 (MQ=255)                                                                                                            acaGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCg                            <  2:405396/140‑1 (MQ=255)                                                                                                            acaGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCg                            <  1:385111/140‑1 (MQ=255)                                                                                                            acaGGGCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCg                            <  1:1089309/140‑1 (MQ=255)                                                                                                               gggCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCGTTa                         <  2:1124090/140‑1 (MQ=255)                                                                                                               gggCAGTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCGTTa                         <  2:779533/140‑1 (MQ=255)                                                                                                                    gTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCGTTATCCCa                    >  1:564953/1‑140 (MQ=255)                                                                                                                    gTGAGCTGTGGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCGTTATCCCa                    <  2:1205925/140‑1 (MQ=255)                                                                                                                          tgtgGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCGTTATCCCAACCCgg              >  1:264030/1‑140 (MQ=255)                                                                                                                          tgtgGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCGTTATCCCAACCCgg              <  2:264030/140‑1 (MQ=255)                                                                                                                            tgGAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGCCGCCGCTTGCGTTATCCCAACCCGgtg            >  2:901405/1‑140 (MQ=255)                                                                                                                             ggAATATTCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCGTTATCCCAACCCGgtgt           <  2:42647/140‑1 (MQ=255)                                                                                                                                    tCATTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCGTTATCCCAACCCGGTGTCATgacg    <  2:1088492/140‑1 (MQ=255)                                                                                                                                      aTTGTCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCGTTATCCCAACCCGGTGTCATgacgac  >  2:671782/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GGTAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGTTTGCGTTATCCCAACCCGGTGTCATGACGAC  >  NC_000913/116965‑117237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑